An In Situ Bioreactor for the Treatment of Petroleum Hydrocarbons in Groundwater

Two pilot tests of an aerobic in situ bioreactor (ISBR) have been conducted at field sites contaminated with petroleum hydrocarbons. The two sites differed with respect to hydrocarbon concentrations. At one site, concentrations were low but persistent, and at the other site concentrations were high enough to be inhibitory to biodegradation. The ISBR unit is designed to enhance biodegradation of hydrocarbons by stimulating indigenous microorganisms. This approach builds on existing Bio‐Sep^® bead technology, which provides a matrix that can be rapidly colonized by the active members of the microbial community and serves to concentrate indigenous degraders. Oxygen and nutrients are delivered to the bioreactor to maintain conditions favorable for growth and reproduction, and contaminated groundwater is treated as it is circulated through the bed of Bio‐Sep^® beads. Groundwater moving through the system also transports degraders released from Bio‐Sep^® beads away from the bioreactor, potentially increasing biodegradation rates throughout the aquifer. Groundwater sampling, Bio‐Traps, and molecular biological tools were used to assess ISBR performance during the two pilot tests. Groundwater monitoring indicated that contaminant concentrations decreased at both sites, and the microbial data suggested that these decreases were due to degradation by indigenous microorganisms rather than dilution or dispersion mechanisms. Taken together, these lines of evidence showed that the ISBR system effectively increased the number and activity of indigenous microbial degraders and enhanced bioremediation at the test sites. © 2013 Wiley Periodicals, Inc.     

Plant nutrient issues for sustainable land application

Interest in plant nutrient issues for sustainable land application of residuals is increasingly driven by environmental concerns. The indicators of concern are P and N in surface waters, nitrate leaching, and emissions of ammonia and greenhouse gases. Federal regulations require residual application rates to be on a N basis at most, and on a P basis when risk of P loss in surface runoff is high. Modeling of mineralization offers the potential for more accurate determinations of potentially available nitrogen (PAN) and quick tests could allow the determination of PAN on residuals immediately before land application. Methods for reducing ammonia emissions from livestock operations and new techniques for quantifying emissions under field conditions are being developed. Calibration and validation of P loss assessment tools is an ongoing concern and the interpretation of edge of field P losses warrants further attention. The solubility of P in residuals and soils can be influenced by various amendments or treatment processes. High available P grains or phytase enzyme supplementation can reduce total and soluble P in animal manures by reducing the need for diet supplementation with inorganic P. The use of synchrotron-based X-ray absorption spectroscopy has identified chemical forms of inorganic P. Considerable progress has been made addressing plant nutrient issues for sustainable land application and interest in this topic will remain strong into the foreseeable future.     

The effect of navigational expertise on wayfinding in new environments

Becoming proficient at navigation in urban environments is something that we all aspire to. Here we asked whether being an expert at wayfinding in one environment has any effect on learning new spatial layouts. Licensed London taxi drivers are among the most proficient urban navigators, training for many years to find their way around a complex and irregularly-laid out city. We first tested how well they could learn the layout of an unfamiliar town compared with a group of non-taxi drivers. Second, we investigated how effectively taxi drivers could integrate a new district into their existing spatial representation of London. We found that taxi drivers were significantly better than control participants at executing routes through the new town, and representing it at a map-like survey level. However, the benefits of navigational expertise were not universal. Compared with their performance in the new town, taxi drivers were significantly poorer at learning the layout of a new area that had to be integrated with their existing knowledge of London. We consider reasons for this picture of facilitation and limitation, in particular drawing parallels with how knowledge acquisition occurs in the context of expertise in general.     

Wastewater effects on a waterhyacinth marsh and adjacent impoundment

A waterhyacinth (Eichhornia crassipes) marsh occupying two-thirds of the basin of a small Florida impoundment has received sewage effluent for nearly 20 years. Water from the marsh flows into an area that is maintained free of waterhyacinths, and is discharged through wells at the far end of the impoundment. A water budget for the basin was estimated, and phosphorus concentrations were measured monthly at three stations in the marsh and at the discharge wells in the lake. Productivity levels were measured monthly where the marsh joins the lake and at the discharge wells. Only 16% of the phosphorus that enters the basin is stored. Gross primary productivity levels in the open-water areas are very high (22 gO₂m²day), but high respiration rates appear to keep the lake in steady-state.