The use of leeches and logit log-linear contingency models to assess and monitor aquatic PCB contamination originating from mid-Canada radar line site 050

Abandoned military sites in northern North America are relics of the Cold War and sources of polychlorinated biphenyls (PCBs). In the late 1990s, the Canadian federal and provincial governments began the cleanup of the mid-Canada radar line in Ontario, Canada. The first site to be remediated was Site 050 (Fort Albany First Nation) in 2001; however, the community remains concerned that contaminants may have moved prior to, during, and after remediation into the Albany River directly adjacent to Site 050. Thus, the Albany River was monitored (1999, 2001, 2002) during the remediation process to determine if the cleanup itself further contaminated the aquatic compartment, using leeches (Haemopis spp.) as bioindicators. Few organochlorines were found in leeches at levels higher than the detection limit, aside from PCBs. Leech data from the present study indicated that PCB levels were significantly higher near Site 050 than the control site upstream, indicating point source contamination from Site 050. The temporal trend in leech contaminant data indicated an increase in PCB contaminant load from 1999 (pre-remediation) to 2001 (immediately post-remediation), but this difference was not statistically significant due to high variances. Nevertheless, logit log-linear contingency modeling did reveal that immediately after cleanup (2001), contaminants (CBs 99, 118, 128, 156, 170, 183) in leeches were detected significantly more frequent than expected. When taken together, leech body burden and frequency of detection data suggest that the remediation process itself further contaminated the aquatic environment, if only temporarily. Lastly, the removal of the terrestrial source of PCBs during remediation did remove the source of aquatic contaminants in that body burden of contaminants in leeches were significantly lower a year after cleanup.     

A study on the self-assembly behavior of dark materials from molasses

We have previously demonstrated that dark materials (DM) in acidified molasses are effectively adsorbed to Amberlite XAD7HP resin and are eluted from the resin with 0.1 M sodium hydroxide. In this paper, we have characterized the self-assembly behavior of molasses DM by using dynamic and static light scattering in combination with isoelectric focusing and infrared absorption spectroscopy in order to better understand the resin adsorption mechanism. One of DM derivatives, X-G2, contained carboxyl and hydroxyl groups and had a weight-average molar mass of 9.39?×?10³ to 4.42?×?10⁴ at pH 2.1–11.5. The aggregates retained their spherical shape over the full pH range and the large gyration radius (66.4–80.0 nm) indicated that the inner structure was loosely packed. Furthermore, X-G2 had an isoelectric point of 1.8, and its density increased sharply at pH 5.9 and then approached a nearly constant value under alkaline conditions. In summary, the self-assembly processes of DM are controlled by intermolecular hydrogen-bonding and hydrophobic interactions. The aggregates adsorb to the resin through hydrophobic interactions and are eluted when excess carboxylate anions are generated.     

Fixation of methylene-blue-treated nervous tissue for histological and ultrastructural studies

The Science of Nature -     

Hydrocarbon and elemental carbon signatures in a tropical wetland: biogeochemical evidence of forest fire and vegetation changes

Evidence of changing vegetation in the tropical wetland (Rawa Danau, west Java, Indonesia) over the past 7428 years is illustrated by elemental (soot) carbon (EC) and n-alkane composition of sedimentary geolipids. In this study, vegetation changes and relevant controlling factors (e.g. forest fire and climate change) were documented on a decadal to centennial scale. The n-alkane composition that changes with depth might record changes in sources of organic matter (OM) in the wetland. The presence of EC (0.01–0.24% of organic carbon: OC) during late (0–1700 cal. year BP) and mid (3500–4500 cal. year BP) Holocene (at depths 0–50 cm, and 160–210 cm) indicated that large-scale forest fires severely affected the tropical vegetation. The hydrocarbon indices (CPI: carbon preference index, MCN: mean carbon number, and HVI: hydrocarbon vegetation index) significantly correlated with one another while a comparison of EC profile with the profiles of hydrocarbon indices indicated that n-alkane composition of the geolipid in lake sediment could record signatures of changes in catchment vegetation. Forest fire and vegetation changes might be related to regional climatic shifts relating to ENSO activity as well as being influenced by human influences.     

Concentrations of dioxins and related compounds in the blood of Fukuoka residents

Blood samples of 152 residents (male 75 and female 77) aged 20-60 years in Fukuoka, Japan, were analyzed for dioxin toxic compounds of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), non-ortho polychlorinated biphenyls (non-ortho PCBs) and mono-ortho polychlorinated biphenyls (mono-ortho PCBs) as well as 35 PCB congeners and 12 chlorinated pesticides by high-resolution gas chromatography/high-resolution mass spectrometry. Average concentrations of dioxin toxic equivalents (TEQ) from PCDDs, PCDFs, non-ortho PCBs, mono-ortho PCBs and their total in the blood of 152 residents were 10.28, 5.56, 7.75, 4.57 and 28.15 ppt in lipid, respectively. These total TEQ levels were comparable to the blood TEQ levels of normal Japanese (n=735). Lipid basis total TEQ levels progressively increased in the males from 17.1 ppt at age 20s to 47.5 ppt at age 50s and in the females from 19.5 ppt at age 20s to 54.5 ppt at age 50s. Dioxin toxic contribution of PCBs to total TEQ was increased from 31% at age 20s to 55% at age 50s. Average concentrations of total 35 PCBs and total 12 pesticides in the blood of 151 residents were 386 and 1079 ppb in lipid, respectively. Average concentrations (ppb in lipid) of total PCBs and total pesticides also progressively increased in the males from 213 and 444 at age 20s to 868 and 2140 at age 50s, respectively, and in the females from 199 and 588 at age 20s to 550 and 1977 at age 50s, respectively. The levels of TEQ, PCBs, hexachlorobenzene, beta-hexachlorocyclohexane, dieldrin, p,p'-dichlorodiphenyldichloroethylene, p,p'-dichlorodiphenyltrichloroethane, trans-nonachlor and cis-nonachlor were positively correlated with each other in most of the combinations, and the highest correlations (r>0.8) were observed between total TEQ and PCBs in both sexes.     

Current uses of the EPA lead model to assess health risk and action levels for soil

The EPA lead model predicts mean blood lead levels and risk of elevated blood lead levels in children based on lead uptake from multiple sources. In the latest model versions, environmental data from individual homes within a community can be used to predict the overall blood lead distribution and percent risk of exceeding a specific blood lead level (i.e. 10 g dl^–1). Recent criteria used by the EPA to evaluate this information include no more than 5% of houses with a greater than 5% lead risk, and a community weighted-average risk below 5%. Environmental (primarily soil) and blood lead data from a residential community near a smelter were used to illustrate recent uses of the model. Scheduled remediation in the community will remove soil for approximately 60% of the houses (i.e. those with lead levels > 1000 mg kg^–1). After remediation, the model results indicate a relatively low community risk (0.5–1.9%), although the percentage of houses with lead risks above 5% ranged from 3 to as high as 13%, depending on the variation in blood lead and assuming the model's 7 g dl^–1 increase in blood lead with each 1000 mg kg^–1 increase in soil lead level. A comparison of the limited blood lead data with soil lead levels below 1000 mg kg^–1, however, indicated no apparent relationship. Given these uncertainties, less invasive actions than additional soil removal (e.g. exposure intervention, monitoring conditions, and follow-up as necessary) may be appropriate under the new EPA guidance for lead in soil.     

The Mid-Canada Radar Line and First Nations' people of the James Bay region, Canada: an evaluation using log-linear contingency modelling to analyze organochlorine frequency data

Abandoned radar line stations in the North American arctic and sub-arctic regions are point sources of contamination, especially for PCBs. Few data exist with respect to human body burden of organochlorines (OCs) in residents of communities located in close proximity to these radar line sites. We compared plasma OC concentration (unadjusted for total lipids) frequency distribution data using log-linear contingency modelling for Fort Albany First Nation, the site of an abandoned Mid-Canada Radar Line station, and two comparison populations (the neighbouring community of Kashechewan First Nation without such a radar installation, and Hamilton, a city in southern Ontario, Canada). This type of analysis is important as it allows for an initial investigation of contaminant data without imputing any values. The two-state log-linear model (employing both non-detectable and detectable concentration frequencies and applicable to PCB congeners 28 and 105 and cis-nonachlor) and the four-state log-linear model (using quartile concentration frequencies for Aroclor 1260, PCB congeners [99,118,138,153,156,170,180,183,187], beta-HCH, p,p'-DDT +p,p'-DDE, HCB, mirex, oxychlordane, and trans-nonachlor) revealed that the effects of subject gender were inconsequential. Significant differences (p < 0.05) between the groups examined were attributable to the effect of location on the frequency of detection of OCs or on their differential distribution among the concentration quartiles. In general, people from Hamilton had higher frequencies of non-detections and of concentrations in the first quartile (p < 0.05) for most OCs compared to people from Fort Albany and Kashechewan (who consume a traditional diet of wild meats that does not include marine mammals). An unexpected finding was that, for Kashechewan males, the frequency of many OCs was significantly higher (p < 0.05) in the 4th concentration quartile than that predicted by the four-state log-linear model, but significantly lower than expected in the 1st quartile for beta-HCH. The levels of PCBs found for women in Fort Albany and Kashechewan were greater than those reported for Dene (First Nation people) and Métis (mixed heritage) of the western Northwest Territories (NWT) who did not consume marine mammals, and for Inuit living in the central NWT (occasional consumers of marine mammals). Moreover, the levels of total p,p'-DDT were greater for Fort Albany and Kashechewan women compared to these same aboriginal groups.     

Degradation of microcystins using immobilized microorganism isolated in an eutrophic lake

The final purpose of our series of studies is to establish a biological removal method of cyanobacteria and their toxic products using immobilized microorganisms that can lyse cyanobacteria and decompose microcystins. To establish the biological removal method in non-point areas and water purification plants, as the first step, we explored bacteria active against the cyanobacterial hepatotoxin microcystin in the present study. Eleven active bacteria were isolated from samples taken from Lakes Tsukui and Sagami, Japan. Among 3 strains (B-9 to B-11) with degradative activity, strain B-9 exhibited the strongest activity. The 16S rDNA sequence of the strain B-9 showed the highest similarity to that of Sphingomonas sp. Y2 (AB084247, 99% similarity). Microcystins-RR and -LR were completely degraded by strain B-9 (SC16) within 1d, which led to an immobilized microorganism with a polyester resin. The degradation of microcystin-RR in a bioreactor using the immobilized strain B-9 was observed and microcystin-RR (> 90%) was completely degraded after 24 h. Microcystin-RR was added to the lake water at regular intervals and the degradation after 24 h was observed in the bioreactor over a 72-d period. An over 80% removal efficiency continued for 2 months, showing that the life of the immobilized B-9 in terms of activity was at least 2 months under the optimized conditions. From these results, this immobilized B-9 is feasible for the practical treatment of microcystins in non-point areas and water purification plants.