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22.
Koji Yachiguchi Noriko Matsumoto Yuki Haga Motoharu Suzuki Chisato Matsumura Masahiro Tsurukawa Toshihiro Okuno Takeshi Nakano Kimi Kawabe Kei-ichiro Kitamura Akira Toriba Kazuichi Hayakawa Vishwajit S. Chowdhury Masato Endo Atsuhiko Chiba Toshio Sekiguchi Masaki Nakano Yoshiaki Tabuchi Takashi Kondo Shigehito Wada Hiroyuki Mishima Atsuhiko Hattori Nobuo Suzuki 《Environmental science and pollution research international》2014,21(10):6365-6372
To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments. 相似文献
23.
Keiko Gion Hideyuki Inui Hideaki Sasaki Yasushi Utani Susumu Kodama Hideo Ohkawa 《Journal of environmental science and health. Part. B》2013,48(7):599-607
Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of β-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige–Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g?1 PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps. 相似文献
24.
Kojima H Takeuchi S Tsutsumi T Yamaguchi K Anezaki K Kubo K Iida M Takahashi T Kobayashi S Jin K Nagai T 《Chemosphere》2011,83(6):753-759
There is a strong need for the development of relatively rapid and low-cost bioassays for the determination of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and dioxin-like polychlorinated biphenyls (dl-PCBs) in environmental and food samples. In this study, we applied a reporter gene assay using DR-EcoScreen cells (DR-cell assay), which is highly sensitive to dioxins, to the determination of PCDD/Fs and dl-PCBs in fish and seafood samples. The PCDD/Fs and dl-PCBs were extracted from homogenated samples (10 g) of 30 fish and shellfish, purified by clean-up procedure using a multilayered silica gel column and an alumina column, and applied to DR-cell assay. Interestingly, the bioanalytical equivalent (BEQ) values obtained from the DR-cell assay [<0.1 ∼ 5.4 pg BEQ g−1 wet weight (ww)] were closely correlated with the toxicity equivalent (TEQ) values from conventional high-resolution gas chromatography/high-resolution mass spectrometry (HRGC-HRMS) analysis (r2 = 0.912), and the slope of regression line was 0.913. Therefore, we multiplied the BEQ values from the DR-cell assay by a conversion coefficient (1.095, the reciprocal of 0.913) to approximate the TEQ values from the HRGC-HRMS analysis. Furthermore, we used this DR-cell assay to perform a prescreening test of PCDD/Fs and dl-PCBs in 16 fish and seafood samples purchased from a supermarket, revealing that a sample from the fatty flesh of a bluefin tuna exceeded 8 pg TEQ g−1 ww (the European Union-tolerance limit). Taken together, these results suggest that the DR-cell assay might be applicable as a rapid and low-cost prescreening method to determine dioxin levels in fish and seafood samples. 相似文献
25.
A method for regional-scale material flow and decoupling analysis: A demonstration case study of Aichi prefecture, Japan 总被引:6,自引:0,他引:6
Junzo Tachibana Keiko Hirota Naohiro Goto Koichi Fujie 《Resources, Conservation and Recycling》2008,52(12):1382-1390
We have developed a method to analyse the annual material flow in a prefecture and have calculated environmental indicators for a prefecture. Material flow analysis (MFA) is important to clarify the structure of a regional society and obtain environmental indicators for a circular society. However, MFA has not advanced in local governments because of few local statistics. We have developed a method to analyse the annual material flow in Aichi prefecture from 1980 to 2000 using an input–output (I–O) table and statistics of Aichi. We have verified the accuracy of this method by comparing its results for 2000 which calculated on the basis of official I–O table for 1995 with the I–O table data for 2000; the correlation coefficient obtained in this case is greater than 0.95. Moreover, by performing MFA, we have estimated the resource consumption and decoupling indicator of each industry in Aichi prefecture from 1980 to 2000. We could obtain more detailed and accurate environmental indicators by using our method. From these results, we could estimate the progress of Aichi prefecture towards a circular society. 相似文献
26.
Ken Inoue Makoto Kanai Yuzo Tanabe Takeo Kubota Catherine D. Kashork Keiko Wakui Yoshimitsu Fukushima James R. Lupski Lisa G. Shaffer 《黑龙江环境通报》2001,21(13):1133-1136
A submicroscopic genomic duplication in Xq22.2 that contains the entire proteolipid protein 1 gene (PLP1) is responsible for the majority of Pelizaeus–Merzbacher disease (PMD) patients. We previously developed an interphase FISH assay to screen for PLP1 duplications in PMD patients using peripheral blood and lymphoblastoid cell lines. This assay has been utilized as a clinical diagnostic test in our cytogenetics laboratory. To expand usage of the interphase FISH assay to prenatal diagnosis of PLP1 duplications, we examined three PMD families with PLP1 duplications utilizing aminiotic fluid samples. In two families the FISH assay revealed fetuses with PLP1 duplications, whereas the other fetus showed a normal copy number of PLP1. Haplotype analyses, as well as an additional FISH analysis using postnatal blood samples, confirmed the results of the prenatal analyses. Our study demonstrates utility of the interphase FISH assay in the prenatal diagnosis of PLP1 duplications in PMD. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
27.
Rafael Vicente de P��dua Ferreira Solange Kazumi Sakata Vera L��cia Keiko Isiki Hissae Miyamoto Maria Helena Bellini Luis Filipe C. P. de Lima J��lio Takehiro Marumo 《Environmental Chemistry Letters》2011,9(2):209-216
The present study investigated the influence of 241Am on microbial growth and the degradation of organic waste. Leachate samples collected in a lysimeter were periodically analyzed
for bacterial growth, under both aerobic and anaerobic conditions. 241Am inhibited bacterial growth, and the degradation of organic matter was delayed in comparison with the control. Minimal inhibitory
concentration assays and survival curves revealed that it inhibits the growth of Pseudomonas putida F1. The assay also revealed that 241Am is more toxic than 238U, Zn2+ and Cd2+. This study further led to the finding of four new radionuclide-tolerant bacterial strains: Flavobacterium spp., Pseudomonas gladioli, Chryseobacterium indologenes and Ochrobactrum anthropi. The survival curves of P. gladioli, C. indologenes revealed that these bacteria are resistant to metal as consortia. 相似文献
28.
Jun Shishido Keiko Yoshii Yoshihiro Takubo Yoshiaki Okamoto Takeshi Yamamoto Yoshiko Kato Tsutomu Nishihara Masaomi Kondo 《Chemosphere》1984,13(2):339-349
An automated survey system on the chemicals in environments by a gas chromatography-mass spectrometry-computer system has been constructed of Three Dimensional Mass Chromatography, Modified Probability Based Matching method, and Self Training Interpretive and Retrieval method. And it was applied to analyze environmental materials, with interesting results. 相似文献
29.
Shuhei Tomita Shin-Ichi Maekawa Mustafizur Rahman Fumi Saito Ryoichi Kizu Keiko Tohi 《毒物与环境化学》2013,95(1):113-124
Epidemiological studies suggest that diesel exhaust particles (DEP) contribute to an increase in allergic diseases. To assess the effects of DEP on the central immune system, mice were exposed to DEP by intraperitoneal (IP) administration. Exposure to DEP resulted in severe thymic involution accompanied by a reduction in the number of thymocytes, especially in cortical CD4+CD8+ double positive and double negative subsets. Core carbon particles associated with a mixture of chemical compounds in DEP did not appear to be responsible for the DEP-induced thymic involution because carbon graphite does not affect neither the number nor the CD4/CD8 profile of thymocytes. Extraction of DEP by ether, acidic and basic solvents showed that several independent fractions including the neutral ether fraction, which contains polycyclic aromatic hydrocarbons (PAH), induced thymic involution. Among major PAH components of DEP, benzo[b]fluoranthene (BbF), benzo[a]pyrene (BaP), and benzo[k]fluoranthene (BkF) were very potent inducers of thymic involution at an ED50 of less than 100?ng per mouse body. Nonetheless, DEP treatment of mice with targeted disruption of genes encoding the aryl hydrocarbon receptor (AHR), AHR nuclear translocator (ARNT), or microsomal epoxide hydolase (mEH) indicated that DEP produced thymic involution even in the absence of PAH-induced AHR/ARNT signal transduction or mEH-mediated PAH catabolism. On the other hand, BaP-mediated thymic involution was completely dependent on AHR, partially dependent on ARNT in T cells, and independent of mEH. These results indicate that DEP-induced thymic involution is mediated both by PAH-AHR/ARNT-dependent and -independent mechanisms. 相似文献
30.
Ronaldo Sousa Tadeusz Zając Dariusz Halabowski Olga V. Aksenova Yulia V. Bespalaya Francisco Carvalho Paulo Castro Karel Douda Janine P. da Silva Noé Ferreira-Rodríguez Juergen Geist Clemens Gumpinger Anna M. Labecka Jasna Lajtner Iga Lewin Manuel Lopes-Lima Alexandra Meira Keiko Nakamura Joana Garrido Nogueira Paz Ondina Małgorzata Ożgo Joaquim Reis Nicoletta Riccardi Spase Shumka Mikhail O. Son Amílcar Teixeira Frankie Thielen Maria Urbańska Simone Varandas Niklas Wengström Katarzyna Zając Alexandra Zieritz David C. Aldridge 《Conservation biology》2023,37(2):e13994
Europe has a long history of human pressure on freshwater ecosystems. As pressure continues to grow and new threats emerge, there is an urgent need for conservation of freshwater biodiversity and its ecosystem services. However, whilst some taxonomic groups, mainly vertebrates, have received a disproportionate amount of attention and funds, other groups remain largely off the public and scientific radar. Freshwater mussels (Bivalvia, Unionida) are an alarming example of this conservation bias and here we point out six conceptual areas that need immediate and long-term attention: knowledge, threats, socioeconomics, conservation, governance and education. The proposed roadmap aims to advance research, policy and education by identifying the most pressing priorities for the short- and long-term conservation of freshwater mussels across Europe. 相似文献