Selection of microalgae for high CO2 fixation efficiency and lipid accumulation from ten Chlorella strains using municipal wastewater

As significant differences in cellular physiology, metabolic potential and genetics occur among strains with morphological similarity, the screening of appropriate microalgae species for effective CO₂ fixation and biodiesel production is extremely critical. In this study, ten strains of Chlorella were cultivated in municipal wastewater influent (MWI) and their tolerance for MWI, CO₂ fixation efficiency and lipid productivity were assessed. The results showed that the biomass concentrations of four strains (Chlorella vulgaris, Chlorella 64.01, Chlorella regularis var. minima and Chlorella sp.) were significantly higher than other strains. When the cultivation systems were aerated with 10% CO₂, Chlorella sp. showed the highest CO₂ fixation efficiency (35.51%), while the highest lipid accumulation (58.48%) was observed with C. vulgaris. Scanning electron microscopy images revealed that the cells of both Chlorella sp. and C. vulgaris kept their normal morphologies after 15 day batch culture. These findings indicated that Chlorella sp. and C. vulgaris have fairly good tolerance for MWI, and moreover, Chlorella sp. was appropriate for CO₂ fixation while C. vulgaris represented the highest potential for producing biodiesel.     

一体式膜-生物反应器中膜污染过程的动态分析

膜污染过程的动态研究对于有效地控制膜污染意义重大。结合膜过滤压差的上升和污染膜表面微观形态的变化 ,对不同污泥浓度和膜通量条件下 ,一体式膜 生物反应器中膜污染过程进行了动态分析。结果表明 ,膜污染初期主要是水中溶解性物质在膜表面附着 ,随后污泥在膜表面沉积。溶解性有机物在膜面的附着 ,对膜过滤压差即膜过滤阻力的变化影响不大 ,而活性污泥在膜表面的大量沉积将导致膜过滤压差迅速上升。污泥浓度愈高 ,膜通量愈大时 ,活性污泥颗粒愈易在膜面沉积。通过停止进出水维持空曝气、降低反应器内污泥浓度或延长膜的停抽时间可以使沉积在膜表面的悬浮污泥脱离膜表面 ,从而使膜过滤能力得到很好的恢复。采用经典的膜污染模型对各运行阶段膜污染模式进行了分析 ,模型拟合结果与电镜观察结果基本吻合。     

解决压载水微生物入侵性传播研究的国际动态

船舶压载水排放造成了地理性隔离水体间的生物入侵，是有害微生物传播的最主要途径。有害水生生物和病原体通过压载水传播，严重威胁侵入国的经济、环境及人类健康，已引起世界范围的广泛关注，许多国家都已开始着手调查外来生物入侵现状，研究管理措施、治理对策及方法。文中对此作一综述。     

护栏伤人事故的行为原因研究

为有效预防护栏卡脖伤人事故的再次发生,统计研究了2008—2016年发生的8起护栏卡脖伤人事故,以事故致因"2-4"模型为分析工具对事故发生的行为原因进行分析,结果表明:事故发生的直接原因包括直接不安全动作即在护栏边休息等,其它不安全动作和不安全物态即形状、间隙大小不合理的护栏等;间接原因是受害者、行人、护栏设计生产者、管理运营者、标准制定者的安全知识、意识、习惯、生理、心理存在问题;根本原因是家庭、社区、公司、生产厂家、管理运营单位、国家标准制定单位等组织安全管理体系的缺欠;根源原因是上述组织的安全文化缺欠。最后,针对事故所涉及的组织分别提出预防对策。     

膜-生物反应器运行条件对膜过滤特性的影响

试验采用膜抽吸压力的上升速率表征运行过程中膜过滤性能的变化。正交试验结果表明,缩短抽吸时间或延长暂停时间和增加曝气量均有利于减缓膜污染,但过短的抽吸时间、过长的暂停时间和过大曝气量不能进一步地减轻膜污染,因此应在保证一定产水量的前提下确定适宜的抽、停时间和曝气量。在污泥龄４０ｄ,污泥浓度稳定在５ｇ／Ｌ左右的条件下,最佳组合操作条件为抽吸１０ｍｉｎ,停抽５ｍｉｎ,曝气量４ｍ＾３／ｈ。３个因素中抽吸时     

Real-world gaseous emission characteristics of natural gas heavy-duty sanitation trucks

As compared to conventional diesel heavy-duty vehicles, natural gas vehicles have been proved to be more eco-friendly due to their lower production of greenhouse gas and pollutant emissions, which are causing enormous adverse effects on global warming and air pollution. However, natural gas vehicles were rarely studied before, especially through on-road measurements. In this study, a portable emission measurement system (PEMS) was employed to investigate the real-world emissions of nitrogen oxides (NOx) (nitrogen monoxide (NO), nitrogen dioxide (NO₂)), total hydrocarbons (THC), carbon monoxide (CO), and carbon dioxide (CO₂) from two liquified natural gas (LNG) China V heavy-duty cleaning sanitation trucks with different weight. Associated with the more aggressive driving behaviors, the vehicle with lower weight exhibited higher CO₂ (3%) but lower NOx (48.3%) (NO₂ (78.2%) and NO (29.4%)), CO (44.8%), and THC (3.7%) emission factors. Aggressive driving behaviors were also favorable to the production of THC, especially those in the medium-speed range but significantly negative to the production of CO and NO₂, especially those in the low-speed range with high engine load. In particular, the emission rate ratio of NO₂/NO decreased with the increase of speed/scaled tractive power in different speed ranges.     

水电建设区的生态环境监测与评价——以向家坝水电站为例

水电工程的建设对区域环境会产生不可逆转的重大扰动,生态环境监测与预警作为大型水电工程建设的重要环节,为工程区域环境问题分析与研究提供详实的数据支撑,对区域生态环境的修复至关重要。以向家坝水电站为例,分析水电工程建设对区域环境产生扰动的特点,结合产生扰动的各环境要素,构建工程施工区环境监测体系。收集、整理相关监测数据,采用环境因子综合评价法对监测的相关数据进行分析和评价,结合各种环境因子的现状以及变化过程、总结区域环境总体状况的变化规律及空间分布。结果表明：自2007年工程大规模动工以来,区域的生态脆弱度大幅度升高,2008年达到顶峰,自2009年起环境开始改善,保持优于2007、2008年的状况;最脆弱区域为主体工程和云天化生产区交接地区,低度脆弱区域为河流及工程量少的加工工程区域;工程扰动最大的区域、当地工业区及交通枢纽集中地带是建设区环境最脆弱的区域,工程建设、工业及交通是建设区的主要扰动因素     

Homology modeling of anti-parathion antibody and its interaction with organophosphorous pesticides and analogues

The mechanism of specific recognition in pesticide immunochemistry was investigated by computer-based strategy, and a rapid method for the identification of antibody specificity was developed. Based on the previously produced anti-parathion monoclonal antibody (mAb), the DNA sequence was analyzed by polymerase chain reaction (PCR). From the translated amino acid sequences, a three-dimensional structure of the antibody was constructed by homology modeling method, and then it was coordinated by 1 ns molecular dynamics under the explicit solvent condition. The stereochemical property and folding quality were further assessed by Procheck and Profile-3D. The self-compatibility score for the antibody model was 98.7, which was greater than the low score 46.2 and close to the top score 102.6. In addition, parathion and several structural analogues were docked to the constructed antibody structure. The docking results showed that the interaction energy (-40.54 kcal/mol) of antibody-parathion complex was the lowest among all the tested pesticides, which accounted for the high specificity of the antibody to parathion and perfectly matched with the experimental data. Moreover, three residues, Phe165, Asp107 and Thr100 were recognized as the most important residues for antibody reacting with parathion. The interaction energy negatively correlated with the antibody specificity.     

DNPH衍生化采样-溶剂解析-高效液相色谱法同时测定木制品中25种醛酮化合物

建立了DNPH衍生化采样-溶剂解析-高效液相色谱测定木制品中醛酮化合物的方法。采样时需在2,4-二硝基苯肼吸附管前串联一只SEP-PAK脱臭氧小柱;采样体积应≤10 L,流量≤400 m L/min。该法对25种醛(酮)化合物对应的衍生物分离效果良好,在0.005~3 mg/L范围内线性良好,相关系数R2≥0.999 6,回收率为85.3%~113.5%,精密度为2.67%~4.56%。采样量为10 L时,25种醛(酮)化合物的检出限和定量限分别≤0.16和0.50μg/m3。     

管流式冲刷腐蚀实验装置的设计及性能试验

研究腐蚀因素对管道内壁腐蚀机理与腐蚀行为的影响,为加速管道内壁腐蚀,自主设计一种管流式冲刷腐蚀实验装置。该装置属于机电一体化产品,包括总体结构设计、控制系统硬件设计和软件设计,其中控制系统PLC为中央处理器,触摸屏为人机控制界面,并对腐蚀因素温度和流速进行调控。另外,对装置进行性能试验。结果显示,温度的控制精度可达到-0.5～+0.8℃,流速的控制精度可达到±0.01 m/s。该装置运行可靠,测试范围较广,装置性能较好,为管道冲刷腐蚀研究奠定实验基础。