Induction of steroidal hydroxylase activity by plant defence compounds in the filamentous fungus Cochliobolus lunatus

We investigated the hypothesis that the endogenous role of the commercially important inducible steroid hydroxylase cytochrome P450s of fungi was in defense against plant toxophores/secondary metabolites. Two plant defense compounds, the aglycones tomatidine and solanidine, the steroidal glycoalkaloid alpha-tomatine and the triterpene saponin beta-escin were tested as inducers of 11beta/14alpha-steroid hydroxylase in the filamentous fungus Cochliobolus lunatus. The extracts of saponins from the roots of Primula veris and green oat leaves were also tested as inducers of 11beta/14alpha-hydroxylation activity in progesterone biotransformation with the same fungus. Induction of steroid hydroxylase and inhibition of activity in some cases support our hypothesis that their endogenous function is in biochemical defence against secondary metabolites. 4-Pregnene-3,11,20-trione was added as a substrate for biotransformation with C. lunatus. We isolated from culture broth 14alpha-hydroxy-4-pregnene-3,11,20-trione, and the hitherto unreported compounds, 7alpha,14alpha-dihydroxy-4-pregnene-3,11,20-trione and 7alpha-hydroxy-pregna-4,8(14)-diene-3,11,20-trione.     

Will Limits of the Earth's Resources Control Human Numbers?

The current world population is 6 billion people. Even if we adopted a worldwide policy resulting in only 2.1 children born per couple, more than 60 years would pass before the world population stabilized at approximately 12 billion. The reason stabilization would take more than 60 years is the population momentum – the young age distribution – of the world population. Natural resources are already severely limited, and there is emerging evidence that natural forces already starting to control human population numbers through malnutrition and other severe diseases. At present, more than 3 billion people worldwide are malnourished; grain production per capita has been declining since 1983; irrigation per capita has declined 12% during the past decade; cropland per capita has declined 20% during the past decade; fish production per capita has declined 7% during the past decade; per capita fertilizer supplies essential for food production have declined 23% during the past decade; loss of food to pests has not decreased below 50% since 1990; and pollution of water, air, and land has increased, resulting in a rapid increase in the number of humans suffering from serious, pollution-related diseases. Clearly, human numbers cannot continue to increase.     

Landscape Patterns of Florida Scrub Jay Habitat Use and Demographic Success

Remote sensing and geographical information systems are used to analyze landscapes important to species of conservation concern. The accuracy of the methods depends on how closely habitat mapping classes are linked to population demography. Habitat use by Florida Scrub Jays ( Aphelocoma c. coemlescens ) was quantified using circular plots. Habitat variation was mapped using high-resolution aerial photography on a site where all Florida Scrub Jays were color-banded. Nest site selection, nest success, yearling production, and breeder survival were measured within Florida Scrub Jay territories. Habitat use was lowest in areas without scrub oaks or areas within 136 m from forests. Open oak, dominated by scrub oaks and open sandy areas, had the highest use and nest success among habitats. Open oak occurred as narrow patches ( <20 m wide) in landscapes dominated by matrix habitat (palmetto-lyonia and swale marshes). Most wide patches (>50 m) of open oak were potential population sources, where reproduction exceeded mortality. Areas with patches of open oak of less than 1 ha were usually population sinks. Open oak occurred as less flammable patches in a landscape subject to frequent fires. Population sources varied temporally and spatially with fires and site potential to support scrub oaks (soils). Analyses of landscape patterns and dynamics indicated that habitat mapping should not only include patches of currently optimal habitat but should also include landscapes associated with open oak. The influences of landscape patterns on habitat use, reproductive success, survival, and territory size can be quantified at different scales starting with attributes associated with habitat patches, nest sites, and territories. Potential mapping errors occur, however, when habitats are used to quantify the areal extent of sources and sinks and similar population attributes important for species persistence.     

Prenatal measurement of the fetal cerebellum and cisterna cerebellomedullaris by ultrasound

The fetal cerebellum can be visualized with ultrasound throughout the second trimester. We describe a technique for measuring the transverse and anteroposterior cerebellar diameters and the measurement of the cisterna magna in the same plane between 14 and 32 weeks gestation. Nomograms for these measurements against gestational age showed good correlation, and narrow confidence limits for the transverse cerebellar diameter. The transverse cerebellar diameter was also measured directly in 79 fetuses after mid-trimester abortion and the measurements obtained were compared with the ultrasound TCD nomogram. Good correlation, was obtained between the post mortem measurements and the ultrasound TCD nomogram. Routine use of these measurements and nomograms should prove valuable in the diagnosis of congenital abnormality of the posterior fossa and may also be of use in assessing the effect of severe intrauterine growth retardation and other insults on cerebellar growth and development. The narrow confidence limits obtained with the TCD nomogram should enable it to be used with confidence in clinical practice.     

Feeding habits of marine cladocerans in the Inland Sea of Japan

Natural food items of five species of marine cladocerans, Evadne nordmanni, E. tergestina, Penilia avirostris, Podon leuckarti and P. polyphemoides, were investigated in the Inland Sea of Japan between April 1986 and May 1987. Gut content examination with SEM (scanning electron microscopy) revealed that feeding was largely limited to centric diatoms and a few exceptions of pennate diatoms and dinoflagellates. No animal remains were detected, and some unidentified materials were also found. Phytoplankton smaller than 35 m in size (cell diameter in centric diatoms and longest dimension in others) was found most frequently in the gut of cladocerans. The role of grazing of marine cladocerans in trophodynamic pathways of the pelagic realm is discussed.     

Incubation time effects on imazaquin desorption as determined by nonequilibrium thin-soil disc flow

Because organic sorption in soil may never reach equilibrium, a thin-disc flow nonequilibrium method may be helpful in understanding herbicide-soil interactions. This research was conducted to (i) determine the influence of incubation time on imazaquin [2-(4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl)-3-quinolinecarboxylic acid] desorption from soil, (ii) examine the influence of solution flow velocities on desorption, and (iii) elucidate the most appropriate kinetic model to describe imazaquin leaching. Soil at 7.5% moisture w/w was treated with imazaquin and incubated for 24, 72, and 168 h. Treated soil was sealed in an in-line filter apparatus and rinsed with 5.0 mM CaCl2 at 0.33, 0.67, or 1.0 mL min(-1). Effluent was collected as 1.0-mL fractions for a total of 50 mL. Flow was stopped for 24 h. When flow resumed, fractions were collected for an additional 15 mL. After the initial desorption, 79% of the imazaquin incubated for 24 h was leached. Increasing incubation time beyond 24 h reduced imazaquin leaching. After both desorption events, 13% of the initially applied imazaquin remained in the soil incubated for 168 h, compared with 7% with soil incubated for 24 h. Elovich and Freundlich kinetics accounted for 98% of the variance observed in the imazaquin desorption curves. First-order and diffusion kinetics accounted for 91% of the variance. Incubating soil for 72 h before desorption reduced the rate of imazaquin desorption by approximately 12%, compared with the 24-h incubation treatment. Imazaquin desorption was not affected by wash solution flow rate. These data suggest that the kinetics of desorption in prolonged desorption events are limited by transport phenomena (i.e., particle and film diffusion).     

Removal of organic matter and nitrogen from river water in a model floodplain

A significant improvement in river water quality cannot be expected unless nonpoint-source contaminants are treated in addition to the further treatment of point-source contaminants. If river water is sprayed over a floodplain, the consequent water filtration through the sediment profile can simultaneously remove organic matter and nitrogen in the water through aerobic and denitrifying reactions. This hypothesis was tested using lysimeters constructed from polyvinyl chloride (PVC) pipe (150 cm long, 15 cm in diameter) packed with loamy sand floodplain sediment. Water was applied to the top of the lysimeters at three different flow rates (48, 54, and 68 mm d(-1)). Concentrations of NO3 and dissolved oxygen (DO), chemical oxygen demand (COD), and redox potential (Eh) in the water were measured as functions of depth after the system reached steady states for both water flow and reactions. At the rate of 68.0 mm d(-1), a reducing condition for denitrification developed below the 5-cm depth due to the depletion of O2 by organic matter degradation in the surface oxidizing layer; Eh and DO were below 205 mV and 0.4 mg L(-1), respectively. At a depth of 70 cm, COD and NO3-N concentration decreased to 5.2 and 3.8 mg L(-1) from the respective influent concentrations of 17.1 and 6.2 mg L(-1). Most biodegradable organic matter was removed during flow and further removal of NO3 was limited by the lack of an electron donor (i.e., organic matter). These results indicate that the floodplain filtration technique has great promise for treatment of contaminated river water.     

Using Science to Assess Environmental Vulnerabilities

Beginning in 1995, the U.S. Environmental Protection Agency (U.S. EPA), Office of Research and Development has focused much of its ecological research in the Mid-Atlantic as part of the Mid-Atlantic Integrated Assessment (MAIA). The goal of MAIA is to improve the assessability of scientific information in environmental decision-making. Following the Environmental Monitoring and Assessment Program (EMAP) whose goal is to guide monitoring that effectively reflects current ecosystem condition and trends, MAIA's second, current, phase of research under the Regional Vulnerability Assessment (ReVA) program is designed to target risk management activities using available data and models. The papers presented here are from a conference held in May 2003 that presented results of research in this second phase of MAIA. The conference was organized into the following topics: 1. Assessing Current Impacts and Vulnerabilities 2. Forecasting Environmental Condition and Vulnerabilities 3. Developing Management Strategies to Optimize the Future, and 4. Assessing and Responding to Environmental Vulnerability.