Effects of pig manure containing copper and zinc on microbial community assessed via phospholipids in soils

Pig manure (PM) is widely used as an organic fertilizer to increase yields of crops. Excessive application of compost containing relatively great concentrations of copper (Cu) and zinc (Zn) can change soil quality. To clarify the effects of different rates of application and to determine the optimal rate of fertilization, PM containing 1,115 mg Cu kg^?1, dry mass (dm) and 1,497 mg Zn kg^?1, dm was applied to alkaline soil at rates of 0, 11, 22, 44, 88, and 222 g PM kg^?1, dm. Phospholipid fatty acids (PLFAs) were used to assess soil microbial community composition. Application of PM resulted in greater concentrations of total nitrogen (TN), NH₄ ⁺-N, NO₃ ^?-N, total carbon (TC), soil organic matter (SOM) but lesser pH values. Soils with application rates of 88–222 g PM kg^?1, dm had concentrations of total and EDTA-extractable Cu and Zn significantly greater than those in soil without PM, and concentrations of T-Cu and T-Zn in these amended soils exceeded maximum limits set by standards in china. Except in the soil with a rate of 11 g PM kg^?1, dm, total bacterial and fungal PLFAs were directly proportional to rate of application of PM. Biomasses of bacteria and fungi were significantly greater in soils with application rates of 44–222 g PM kg^?1, dm than in the soil without PM. SOM, TC and EDTA-Zn had the most direct influence on soil microbial communities. To improve fertility of soils and maintain quality of soil, rate of application should be 22–44 g PM kg^?1 dm, soil containing Cu and Zn.     

电旋风除尘模型实验研究

本研究试图将电除尘器与旋风除尘器组合为一体,以求取得较高的除尘效率,并利用旋转气流实现自动清灰。本文介绍了切向进气直流式电旋风除尘的模型试验,通过实测不同工况下的除尘效率和压力损失,应用经典除尘理论,建立了相应的数学模型。     

采用两级物化法去除黄磷废水中的氟化物

采用向含氟废水中加入适量石灰和聚合氯化铝，然后投加适量钙盐、磷酸和聚丙烯酰胺的二级处理工艺除氟，通过控制合适的pH、钙盐和磷酸的投加量、反应时间、反应温度、搅拌强度和絮凝剂的投加量，可使黄磷废水中氟的质量浓度降至5mg/L，氟的去除率达到99％以上，出水中氟的质量浓度达到GB8978－1996一级排放标准。     

Acute toxicity and genotoxicity of two novel pesticides on amphibian, Rana N. Hallowell

Imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitro-imidazolidin-2-ylideneamine] and RH-5849 [2'-benzoyl-l'-tert-butylbenzoylhydrazinel] are two pesticides used in China since 1992. In the present study we conducted acute toxicity test, micronucleus (MN) test and comet assay of the two pesticides on amphibian, Rana N. Hallowell, a sensitive organism suitable for acting as the bio-indicator of aquatic and agricultural ecosystems. The values of LC50-48 h of imidacloprid were found to be 165 mg l(-1) for tadpoles of Rana limnocharis and 219 mg l(-1) for tadpoles of Rana N. Hallowell. On the other hand, RH-5849 showed no acute toxicity to tadpoles during the 96 h exposure even it was saturated in the test solutions. There were significant differences in the MN frequencies between the negative controls and the treated groups at the dose of 8 mg l(-1) for imidacloprid (p < 0.05) and 40 mg l(-1) for RH-5849 (p < 0.01). Comet assay found significant differences (p < 0.01) in the distributions of DNA damage grades between the negative controls and groups treated in vitro with 0.05, 0.1, 0.2 and 0.5 mg l(-1) of imidacloprid and 5, 25, 50 and 100 mg l(-1) of RH-5849, respectively. DNA damage scores increased with the exposure levels of the two pesticides and dose-effect relationships were observed for both imidacloprid (r2 = 0.92) and RH-5849 (r2 = 0.98). The MN test and comet assay revealed potential adverse effects of the two pesticides on DNA in the erythrocytes of amphibians in aquatic and agricultural ecosystems.     

Analysis of microcystins in cyanobacteria blooms and surface water samples from Meiliang Bay,Taihu Lake,China

Taihu Lake is the third largest freshwater lake in China. In recent years, the water pollution of cyanobacteria blooms has become a severe problem in this area. Microcystins (MCs) are an important group of toxic compounds mainly produced by some cyanobacteria species and have both acute and chronic hepatotoxic effects on animals and humans. This paper presents the first data on the identification and detection of MCs in both natural occurring cyanobacteria blooms and surface water samples (0-0.5 m), collected from Meiliang Bay, Taihu Lake, China. A conventional method for extraction and isolation of MCs from cyanobacteria blooms was applied. High-performance liquid chromatography (HPLC) analysis showed that the main toxic component in the cyanobacteria materials was MC-LR. The monoclonal antibody (mAb) against MC-LR produced by hybridoma technique was employed for direct competitive ELISA to detect the concentrations of MCs in bloom and water samples collected in 2001. The results not only revealed the presence of MCs but also temporal variations of MCs levels of three sampling stations in Meiliang Bay in 1 year. It is obvious that the MC contents were relatively higher during warm months and related with the status of eutrophication. Our study indicates the threat associated with MCs in water body of Taihu Lake. To prevent the MCs potential hazard on public health in this area, some necessary measures of monitoring and control of growth of cyanobacteria are urgently needed.     

丹江口库区湿地自然保护区现状及保护对策

湖北丹江口库区湿地自然保护区是"内陆湿地和水域生态系统类型"自然保护区,但受人为活动的影响,水文情势改变,保护区的湿地生态系统受到一定的威胁。基于丹江口库区湿地自然保护区现状,从合理调整保护区范围、全面加强湿地生态建设、加强环境污染防治、建立有效的湿地保护管理协调机制、加强湿地保护知识的宣传等方面,构建和谐生态,实现人与自然协调发展。     

Quantitative analysis of riparian herbaceous community in Liaohe River Conservation Area and its tributaries北大核心CSCD

Research on community characteristics of riparian herbs is an important scientific basis of riparian vegetation rehabilitation. This study aimed to investigate the species diversity and quantitative characteristics of riparian herbs in Liaohe River Conservation Area and its tributaries. Herbaceous communities were investigated by sample line method. Group average clustering analysis and detrended correspondence analysis (DCA) were used to find the major environmental factors affecting the distribution pattern of riparian herbaceous communities. The result showed altogether 154 herbaceous species, belonging to 40 families and 96 genera. The riparian herbaceous species diversity was found to be correlated to river slope, sinuosity and stream order, being lower in rivers of mountain area than in rivers of plain area, and higher in tributaries than in Liaohe River Conservation Area. Clustering analysis classified the herbaceous communities into 23 types, being dominated by hygrophytes including communities of Echinochloa crusgalli, Oenanthe javanica, Polygonum hydropiper, Murdannia keisak, Artemisia selengensis, Scirpus triqueter, Heleocharis soloniensis, Pycreus sanguinolentus, Cyperus fuscus, Phragmites australis, Polygonum amphibium, Carex diandra + Artemisia selengensis and Carex diandra + Rorippa islandica. Correlation analysis between DCA ordination axes and environmental factors showed that the altitude, river slope, sinuosity and stream level were the major environmental factors affecting the distribution pattern of herbaceous communities in the riparian zone of Liaohe River Conservation Area and its tributaries. The results of CCA showed that the contribution ratio of stream level was the highest, followed by altitude and slope, with sinuosity the last. The results suggested that riparian herbaceous characteristics are affected by the comprehensive force of altitude, river slope, sinuosity and stream order, and that Calamagrostis epigeios, Triarrhena sacchariflora and Phragmites australis are suitable species for riparian vegetation rehabilitation.     

基于esp基因和JCV标记的MST方法在五大流域典型水源地中的应用

非点源污染目前已经成为影响水体环境的重要污染。微生物源示踪技术(microbial source tracking,MST)是解决非点源污染的一项新技术,它可以确定污染的宿主来源。肠球菌esp基因和多瘤病毒JCV可以作为检测水体中人源粪便污染的分子标记,其灵敏度和特异性都很高。为分析五大流域水源地是否受到人源粪便的污染,对辽河、海河、淮河、长江和黄河五大流域典型水源地水样进行采集和检测,选取人源粪便特异病原微生物肠球菌的esp基因和多瘤病毒JCV建立了相应的MST分子检测方法。结果表明,五大流域的典型水源地采样点均有可能受到了人源粪便的污染,可为当地相关部门提供技术支持和数据参考。     

Cu–Mn–Ce ternary mixed-oxide catalysts for catalytic combustion of toluene

Cu–Mn, Cu–Mn–Ce, and Cu–Ce mixed-oxide catalysts were prepared by a citric acid sol–gel method and then characterized by XRD, BET, H₂-TPR and XPS analyses. Their catalytic properties were investigated in the toluene combustion reaction. Results showed that the Cu–Mn–Ce ternary mixed-oxide catalyst with 1:2:4 mole ratios had the highest catalytic activity, and 99% toluene conversion was achieved at temperatures below 220°C. In the Cu–Mn–Ce catalyst, a portion of Cu and Mn species entered into the CeO₂ fluorite lattice, which led to the formation of a ceria-based solid solution. Excess Cu and Mn oxides existed on the surface of the ceria-based solid solution. The coexistence of Cu–Mn mixed oxides and the ceria-based solid solution resulted in a better synergetic interaction than the Cu–Mn and Cu–Ce catalysts, which promoted catalyst reducibility, increased oxygen mobility, and enhanced the formation of abundant active oxygen species.