Influence of aerobic and anaerobic conditions on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in Luria-Bertani broth, farm-yard manure and slurry

The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strain of the enteropathogens at 10(7) cells g(-1) dry weight. Survival data was fitted to the Weibull model. The survival curves in aerobic conditions generally showed a concave curvature, while the curvature was convex in anaerobic conditions. The estimated survival times showed that E. coli O157:H7 survived significantly longer under anaerobic than under aerobic conditions. Survival ranged from approximately. 2 weeks for aerobic manure and slurry to more than six months for anaerobic manure at 16 °C. On average, in 56.3% of the samplings, the number of recovered E. coli O157:H7 cells by anaerobic incubation of Petri plates was significantly (p < 0.05) higher in comparison with aerobic incubation. Survival of Salmonella serovar Typhimurium was not different between aerobic and anaerobic storage of LB broth or manure as well as between aerobic and anaerobic incubation of Petri dishes. The importance of changes in microbial community and chemical composition of manure and slurry was distinguished for the survival of E. coli O157:H7 in different oxygen conditions.     

THE RELATIONSHIP BETWEEN COMMERCIAL AND RECREATIONAL USE OF NAVIGATION LOCKS ON THE UPPER MISSISSIPPI RIVER1

ABSTRACT Navigation locks on the Upper Mississippi River currently receive heavy use from both commercial barge traffic and recreational craft. Multiple regression analysis of lockage statistics suggests a critical level may exist, below which barge traffic volume has little impact on recreational lock use, but above which it physically constrains recreational lockage. The capacity of the Upper Mississippi River for lock chambers at Alton, Illinois. This may adversely impact recreational use of locks by raising commercial traffic levels above the critical level at several other locks on the river.     

Integrating information for better environmental decisions

As more is learned about the complex nature and extent of environmental impacts from progressive human disturbance, scientists, policy analysts, decision makers, educators, and communicators are increasingly joining forces to develop strategies for preserving and protecting the environment. The Eco-Informa Foundation is an educational scientific organization dedicated to promoting the collaborative development and sharing of scientific information. The Foundation participated in a recent international conference on environmental informatics through a special symposium on integrating information for better environmental decisions. Presentations focused on four general themes: (1) remote sensing and data interpretation, including through new knowledge management tools; (2) risk assessment and communication, including for radioactively contaminated facilities, introduced biological hazards, and food safety; (3) community involvement in cleanup projects; and (4) environmental education. The general context for related issues, methods and applications, and results and recommendations from those discussions are highlighted here.     

Nitrate Leaching by Atmospheric N Deposition to Upper Groundwater in the Sandy Regions of The Netherlands in 1990

Anthropogenic increase in atmospheric nitrogen (N) deposition in nature areas results in nitrate leaching to groundwater, threatening its quality. Member states of the European Union are obliged to reduce groundwater nitrate concentrations and to monitor this reduction. The relationship between N deposition and groundwater nitrate concentrations is quantified using a field survey and geographical information. Nitrate concentrations of the uppermost metre of groundwater in nature areas in the sandy regions in 1990 were related to geographical data by means of regression analysis. In this way nitrate concentrations could be explained by potential ammonia deposition, soil type, vegetation and land use. We found that about 35% of 54 kg ha(-1) a(-1) atmospheric N deposition was leached to the upper groundwater as nitrate, resulting in a mean NO3 concentration of about 30 mg L(-1). The critical N load for exceeding the EC limit value (50 mg L(-1)) in the sandy regions of The Netherlands composed of natural vegetation will be about 80 kg ha(-1) a(-1). Leaching is less than expected for nature areas but comparable with leaching of N surpluses in pastures in The Netherlands. A reduction in nitrate leaching by 25% or more can currently be detected via a new field survey.     

How honeybees perceive communication dances,studied by means of a mechanical model

Summary A mechanical model of a dancing honeybee was used to investigate the role of various components of the wagging dance in the transfer of information to follower bees. The model simulates the dance, carries a scent, and has an acoustic near-field similar to that of live dancers. The movements of the model are controlled by a computer, and selected components of the dance can be manipulated independently of others. The number of bees approaching scented baits at various distances and directions from the hive was observed, both during simulated normal dances and dances in which different components provided potentially conflicting information about the location of the food. The results indicate that the wagging run is the master component of the dance. The figure-of-eight dance path does not seem to convey information. Both sound and wagging must be present in the dance, but no specific roles were found for these components. Both sound and wagging convey information about distance and direction, and they appear to be largely redundant. Offprint requests to: A. Michelsen     

A WATERSHED-LEVEL ECOLOGICAL RISK ASSESSMENT METHODOLOGY1

ABSTRACT: We present an ecological risk assessment methodology at the watershed level for freshwater ecosystems. The major component is a pollutant transport and fate model (a modified EUTROMOD) with an integrated uncertainty analysis utilizing a two-phase Monte Carlo procedure. The uncertainty analysis methodology distinguishes between knowledge uncertainty and stochastic variability. The model assesses the ecological risk of lentic (lake) ecosystems in response to the stress of excess phosphorus resulting in eutrophication. The methodology and model were tested on the Wister Lake watershed in Oklahoma with the lake and its trophic state as the endpoint for ecological risk assessment. A geographic information system was used to store, manage, and manipulate spatially referenced data for model input.