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As a new threat to environment all through the world, perchlorate (ClO(4)(-)) was predominantly a thyrotoxin, and its toxic manifestations in non-thyroid were also documented. However, little is known about the effects of ClO(4)(-) on cell and organelle. Therefore, the present study was designed to investigate the effects of ClO(4)(-) on hepatocytes and mitochondria isolated from Carassius auratus from the direct viewpoint of energy by using the microcalorimetric method. The metabolic thermogenic curves of hepatocytes and mitochondria at 25°C were obtained. And the thermokinetic parameters, such as growth rate constant (k), inhibitory ratio (I), maximum thermal power (P(max)) and total thermal effect (Q(total)) have been calculated. The results indicated that the toxicity of ClO(4)(-) on hepatocytes was relevant to the concentration of ClO(4)(-). However, 10-100mgL(-1)ClO(4)(-) stimulated the metabolic activity of mitochondria and the toxicity of ClO(4)(-) on mitochondria only occurrenced when treated with higher concentration of ClO(4)(-). This study shown that mitochondria has a major impact on the metabolic thermogenic of hepatocytes, but not the only factor. Meanwhile, it demonstrated that microcalorimetry was a powerful tool for understanding biological processes and studying on the toxic action of environmental contaminants in cell or subcellular level. 相似文献
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利用ICP-AES分析了潞城市采暖期和非采暖期4个不同功能区PM10样品中16种化学元素,对不同元素的时空分布特征进行了研究,并采用富集因子和主成分分析初步研究了潞城市PM10中元素的主要来源。结果表明,潞城市PM10中重金属污染较为严重,且各元素在采暖期的平均浓度均明显高于非采暖期。PM10中Ca、V、Cr、As、Ni、Mn、Cu、Zn、Al和Pb的富集因子EF〉10,主要来源于人为污染;而Na、Mg、Si、Fe和K的EF〈10,除部分来自人为活动外,主要来自土壤风沙等自然来源。主成分分析结果显示,潞城市PM10中元素的主要来源按贡献率大小依次为:煤烟尘和工业粉尘50.39%,自然源34.37%和机动车尾气15.24%。 相似文献
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Kinmen is located in the southwest of Mainland China. Groundwater supplies 50% of the domestic water use on the island. Residents
of Kinmen drink groundwater over the long term because surface water resources are limited. Nitrate–N pollution is found and
distributed primarily in the western part of groundwater aquifer whereas saline groundwater is distributed to the northeastern
Kinmen. This work applied the DRASTIC model to construct the vulnerability map of Kinmen groundwater. MT3D was then used to
evaluate the contamination potential of nitrate–N. The health risk associated with the ingestion of nitrate–N contaminated
groundwater is also assessed. The results from DRASTIC model showed that the upland crop and grass land have high contamination
potential, whereas the forest, reservoir and housing land have low contamination potential. The calibrated MT3D model inversely
determined the high strength sources (0.09–2.74 kg/m2/year) of nitrate contaminant located in the west to the north west area and required 2–5 years travel time to reach the monitoring
wells. Simulated results of MT3D also showed that both the continuous and instantaneous contaminant sources of nitrate–N release
may cause serious to moderate nitrate contamination in the western Kinmen and jeopardize the domestic use of groundwater.
The chronic health hazard quotient (HQ) associated with the potential non-carcinogenic risk of drinking nitrate–N contaminated groundwater showed that the assessed
95th percentile of HQ is 2.74, indicating that exposure to waterborne nitrate poses a potential non-cancer risk to the residents of the island.
Corrective measures, including protecting groundwater recharge zones and reducing the number of agricultural and non-agricultural
nitrogen sources that enters the aquifer, should be implemented especially in the western part of Kinmen to assure a sustainable
use of groundwater resources. 相似文献