Retention of functional chloroplasts in some sacoglossans from the Indo-Pacific and Mediterranean

Previous studies on kleptoplasty in sacoglossans have used different methodology to investigate how long the sacoglossans are able to keep photosynthetically active (functional) chloroplasts. In this study we have used Pulse Amplitude Modulated Fluorometry to measure the quantum yield of charge separation in photosystem II in dark acclimated cells (Φ_IIe) to detect the status of photosynthetic activity. Seven species of sacoglossa, Plakobranchus ocellatus, Elysia timida, Elysia sp, Elysia tomentosa, Thuridilla carlsoni, T. lineolata and Elysiella pusilla, were investigated regarding their ability to retain functional chloroplasts (RFC). The results show three different levels of RFC’s where P. ocellatus has the longest RFC for more than 11 months, E. timida with a RFC 1/4 than P. ocellatus (almost 3 months) and the rest with RFC’s down to 1/22 of P. ocellatus (up to 15 days). Based on these results, and compared to previous studies, eight different levels of retention abilities of non-functional and functional chloroplasts in sacoglossans are proposed. As far as we know, this is a novel method studying chloroplast functionality in sacoglossans.     

Reef coral reproduction in the eastern Pacific: Costa Rica, Panamá and Galápagos Islands (Ecuador). IV. Agariciidae, recruitment and recovery of Pavona varians and Pavona sp.a

The reproductive ecology of two eastern Pacific zooxanthellate coral species was examined as part of a continuing series of studies relating bleaching/mortality events caused by the El Niño–Southern Oscillation disturbance, and is described for study sites in Costa Rica, Panamá, and the Galápagos Islands (Ecuador). This study deals with the sibling agariciid species Pavona varians and Pavona sp.a over a 13?yr period (1985 to 1997). Both Pavona species are broadcast-spawners with some gonochoric, but mostly sequential hermaphroditic colonies. Minimum colony sizes (and ages) at first reproduction were 5?cm (5?yr) and 3?cm (2 to 3?yr), respectively, in P. varians and Pavona sp.a. In the Panamá and Galápagos populations, gonochoric colonies spawn eggs or sperm at least monthly. Six fecundity attributes were not significantly different in the two species, but the eggs of P. varians are white to beige and positively buoyant, and those of Pavona sp.a are dark green and neutrally to negatively buoyant. Eggs of both species lack zooxanthellae. Both species are reproductively active year-round, with maximum activity in the dry season in the nonupwelling Gulf of Chiriquí, and in the wet season in the upwelling Gulf of Panamá. Spawning is predominantly during full moon, and possibly also at new moon at most study sites. Spawning in P. varians and Pavona sp.a is 12?h out of phase, with the former species spawning ～1?h before sunrise and the latter about 1?h after sunset. The fecundity of Pavona spp. at Caño and the Galápagos Islands was much greater (19?900 to 27?900 eggs cm^?2?yr^?1) than at all Panamá sites (14?800 to 19?800 eggs cm^?2?yr^?1). Intraspecific crosses in both species resulted in swimming planula larvae after 25 to 36?h. Recruitment of P. varians was highest in Panamá, moderate in Costa Rica, and nil in the Galápagos Islands, matching, respectively, the contributions of P. varians to the pre-1982/1983 El Niño coral-population abundances in these areas. Recruitment success of P. varians at Uva Island was significantly related to maximum monthly positive sea surface-temperature (SST) anomalies that occurred in the year preceding recruitment over the period 1982 to 1996; recruitment failed when SST anomalies exceeded 1.6 to 1.9?C° during the severe ENSO events of 1982/1983 and 1997/1998.     

AN EXAMINATION OF THE MECHANISMS CONTROLLING GROUNDWATER GRADIENTS IN HYPER-ARID REGIONAL SEDIMENTARY BASINS1

ABSTRACT: Although evidence of modern recharge in the North African and Arabian sedimentary basin aquifers exists, it is difficult to determine the volume of recharge. Also, from the evidence of regional groundwater gradients, the flow within the aquifers seems to be appreciably greater than one would intuitively expect. A hypotehtical model embodying the characteristics of the aquifers has been used to investigate the likely significance of various possible flow mechanisms. It is shown that while dewatering in the unconfined area can possibly contribute to flows for a considerable period of time, the maintenance of water levels in the unconfined zone must be the result of modern recharge. It is also shown that recharge depths of less than 10 mm per annum are sufficient given suitable aquifer parameters. Results for various combinations of aquifer parameters and configurations are given, including layered aquifers and the effects of restricted oufflows. Comparisons are made using a “bench mark” example. The work indicates that there is little point in carrying out conventional hydrological balance studies in hyper-arid areas and that, instead, more emphasis should be placed upon good groundwater hydrographic data and modeling.     

INTERSTATE AND INTERNATIONAL AQUIFERS1

Many important groundwater aquifers cross state and national boundaries. The flow of water in these aquifers is not influenced by the boundaries but may be materially influenced by mans activities on one or both sides of a boundary. Interstate and international problems may develop because of excessive groundwater lowering on one side of a boundary affecting water users on the opposite side of the line. Similarly, intensive groundwater development along a surface stream may influence the amount of surface water that flows across a boundary. A third type of problem may develop when pumping on one side of the boundary induces poor quality water into an aquifer on the other side of the boundary. Several specific interstate and international aquifer problems are briefly described.     

Die Lösung von Nutzungskonflikten aufgrund flächenhafter Bodenkontaminationen mit Hilfe Geografischer Informationssysteme und Mitteln der Geostatistik

Goal and Scope

By means of Geographical Information Systems (GIS) and other geostatistical methods, the extensive heterogeneous data materials of large area soil contaminations are re-evaluated to eliminate exploitation conflicts and investment obstacles.

Methods

The mapping of relevant frame criteria generates the basis to solve the conflicts, such as types of exploitation of field areas, development plans, protected areas, flooded areas, etc. as well as their geo-referencing in the Geographical Information System. The critical examination of data age and quality as well as the geostatistical evaluation by means of semivariogram analysis and Kriging technique are essential prerequisites for the determination of areas related to utility and exceeding test values.

Results and Conclusions

As a result, one can modify the hazardous situation and can provide recommendations for an optimal elimination of hazards, mostly in the framework of scheduled measures in terms of town planning.

Outlook

On the basis of the results obtained, further exploitation or monitoring work can clearly be optimized.