Production of the monoclonal antibody against tylosin for immunoassay of macrolide antibiotics in milk

The objective of the present study was to develop an immunoassay for multi-determination of the residues of four macrolide antibiotics in milk. Tylosin was derivatized with 6-aminohexanoic acid to synthesize a hapten that was used to produce the monoclonal antibody. The obtained monoclonal antibody simultaneously recognized tylosin, acetylisovaleryltylosin, tilmicosin and desmycosin with crossreactivities of 100%, 91%, 49% and 76%, respectively. Then an indirect competitive immunoassay was developed to determine the four analytes in milk simultaneously. The limits of detection for the four analytes were in the range of 5.5–11.7 ng/mL depending on the compound. The recoveries of the four analytes from fortified blank milk were in the range of 78%–96% with coefficients of variation lower than 11.4%. The developed immunoassay could be used as a practical tool for rapid screening the residues of the four anlytes in milk.     

Co-metabolic transformation of the neonicotinoid insecticide imidacloprid by the new soil isolate Pseudoxanthomonas indica CGMCC 6648

A new imidacloprid (IMI) degrading bacterium Z-9 (deposited number CGMCC 6648) was isolated and identified as Pseudoxanthomonas indica by 16S rRNA gene analysis. Two metabolites were identified as olefin and 5-hydroxy IMI by liquid chromatography-mass spectrometry and nuclear magnetic resonance analysis. P. indica CGMCC 6648 degraded 70.1% of IMI (1.22 mmol L^?1) and formed 0.93 mmol L^?1 5-hydroxy IMI and 0.05 mmol L^?1 olefin IMI in 6 days and in the presence of 100 mmol L^?1 glucose. The half-life of IMI degradation was 3.6 days. P. indica CGMCC 6648 transforms IMI via a co-metabolism mechanism and different carbohydrates have significant effects on 5-hydroxy IMI formation, whereas different organic acids have substantial effects on olefin IMI production. Lactose is the best co-substrate for IMI degradation and 5-hydroxy IMI formation with 0.77 mmol L^?1 degraded and 0.67 mmol L^?1 formed in 48 h, respectively. Pyruvate is the best co-substrate for olefin IMI formation with 0.17 mmol L^?1 produced in 96 h for all carbon sources tested. Pyruvate significantly stimulates the conversion of 5-hydroxy IMI to olefin IMI, whereas glucose slightly inhibits this reaction. P. indica CGMCC 6648 rapidly degrades IMI and forms olefin IMI, which may enhance its potential for biodegradation of IMI and increase its insecticidal activity, which can decrease the IMI dosage required.     

火焰原子吸收光度法测定水中铝的方法改进

研究了在盐酸介质中，壬基酚聚氧乙烯-7醚（NP-7）活化下，火焰原子吸收光度法测定环境水体中铝的方法改进。在25mL容量瓶中，加入5．0mL体积分数为50％的盐酸、2．0mL质量浓度为0．01g／mL NP-7和4．0mL质量浓度为75．0μg/mL的铝标准溶液，在原子吸收分光光度计的最佳测定条件下测定吸光度。根据吸光度与铝质量浓度绘制了工作曲线，线性范围3．0～24．0μg/mL，检出限1．32μg/mL。该法用于环境水体中铝含量的测定，加标回收率为94．4％～101．4％，最大相对标准偏差5．8％，方法对比最大相对误差4．1％。     

浙江拔茅银矿成矿地球化学条件及成矿物质来源

拔茅银矿为一赋存于中生代陆相火山岩中的浅成热液矿床。本文通过该矿床矿物中的气—液包裹体,S、Pb、H、O等稳定同位素组成,矿物共生组合稳定存在的热力学条件,稀土元素地球化学特征,岩石的含矿性等方面的研究,阐述了其成矿地球化学条件和成矿物质来源。     

Effects of plant density on the photosynthetic and chloroplast characteristics of maize under high-yielding conditions

The Science of Nature - Knowledge on animal personality has provided new insights into evolutionary biology and animal ecology, as behavioural types have been shown to affect fitness. Animal...     

废活性炭微波再生新工艺的研究

提出用微波加热一二氧化碳活化法再生乙酸乙烯合成用触媒载体废活性炭工艺。采用条件实验法研究了活化时间、二氧化碳流量和微波功率对活性炭碘吸附值，亚甲基蓝吸附值和再生得率的影响，得到微波辐射加热二氧化碳活化再生乙酸乙烯用触媒载体废活性炭的最佳工艺条件为活化时间25min，二氧化碳流量0．2L／min，微波功率700w。在此条件下制得的活性炭碘吸附值为1158．02mg／g、亚甲基蓝吸附值为240mv,／g、得率为74．19％。并对活性炭进行了比表面积的测定和孔结构的分析，活性炭的比表面积为1308．13m^2／g，总孔容为0．76mL／g。     

催化光度法测定桂林风景点水中痕量铁(Ⅲ)

本文以过氧化氢-甲基红,在活化剂α、α~1-联吡啶存在下,建立了催化光度法测定痕量铁(Ⅲ)的方法,其灵敏度为2.68×10~(-11)gFe(Ⅲ)/ml,线性范围为0～0.6μgFe(Ⅲ)/25ml。用于桂林风景点水中痕量铁测定,结果令人满意。     

重大工业危险源(罐区)监控预警系统

系统建立了管理软件和硬件监控相结合的安全生产监控预警系统，在安全管理的基 础上，把罐区诸多的危险参数给予实时监测、报警和控制。本系统实现了生产过程控制与安全监控 相结合，安全监测报警与事故预案相结合，既满足了生产控制又实现了动态的安全管理，对安全生产 具有较大意义。     

制定粉尘卫生标准的新趋势——介绍欧洲的经验并提出几点建议

介绍了近年来欧洲国家新的粉尘卫生标准及其监测、管理方法,分析其制定粉尘卫生标准的经验,并与我国的粉尘卫生标准情况做了初步的比较,提出了建立我国新分级分类粉尘卫生标准体系的建议。     

Annual average radon concentrations in California residences.

A study was conducted to determine the annual average radon concentrations in California residences, to determine the approximate fraction of the California population regularly exposed to radon concentrations of 4 pCi/l or greater, and to the extent possible, to identify regions of differing risk for high radon concentrations within the state. Annual average indoor radon concentrations were measured with passive (alpha track) samplers sent by mail and deployed by home occupants, who also completed questionnaires on building and occupant characteristics. For the 310 residences surveyed, concentrations ranged from 0.10 to 16 pCi/l, with a geometric mean of whole-house (bedroom and living room) average concentrations of 0.85 pCi/l and a geometric standard deviation of 1.91. A total of 88,000 California residences (0.8 percent) were estimated to have radon concentrations exceeding 4 pCi/l. When the state was divided into six zones based on geology, significant differences in geometric mean radon concentrations were found between several of the zones. Zones with high geometric means were the Sierra Nevada mountains, the valleys east of the Sierra Nevada, the central valley (especially the southern portion), and Ventura and Santa Barbara Counties. Zones with low geometric means included most coastal counties and the portion of the state from Los Angeles and San Bernardino Counties south.