Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC) granules

The biologic activated carbon (BAC) process is widely used in drinking water treatments. A comprehensive molecular analysis of the microbial community structure provides very helpful data to improve the reactor performance. However, the bottleneck of deoxyribonucleic acid (DNA) extraction from BAC attached biofilm has to be solved since the conventional procedure was unsuccessful due to firm biomass attachment and adsorption capacity of the BAC granules. In this study, five pretreatments were compared, and adding skim milk followed by ultrasonic vibration was proven to be the optimal choice. This protocol was further tested using the vertical BAC samples from the full-scale biofilter of Pinghu Water Plant. The results showed the DNA yielded a range of 40 μg·g^-1 BAC (dry weight) to over 100 μg·g^-1 BAC (dry weight), which were consistent with the biomass distribution. All results suggested that the final protocol could produce qualified genomic DNA as a template from the BAC filter for downstream molecular biology researches.     

Mitigation and remediation technologies for organic contaminated soils

Organic contaminated soils have become a widespread environmental problem, which may lead to a great threat to the quality of agricultural production and to human health. Physical, chemical, and biological technologies have been employed for the mitigation and remediation of organic contaminated soils. This paper reviews the progress of mitigation and remediation technologies for organic contaminated soils and suggests two different strategies for the mitigation of ’slightly-contaminated’ agricultural soils and the remediation of ‘heavily-contaminated’ soils/sites, respectively. On this basis, directions for future research in this field are suggested.     

Perception of people for the risk of Tianwan nuclear power plant

A questionnaire survey of residents’ risk perceptions related to Taiwan nuclear power plant in China was carried out to explore the determining factors that affect individual risk perception. This study proposed to pursue a more comprehensive understanding of factors that affected individual risk perception to nuclear power plants. Covariance structure analysis was conducted using risk perceptions of nuclear power as dependent variable and including interest and knowledge levels of nuclear power, acceptability, benefit perception, trust in nuclear power operation, and trust in government as independent variables. The use of the hypothesis of Elaboration likelihood model (ELM) was also proposed. The results showed that persons with higher levels of interest and knowledge of nuclear power had their own perceptions of risk closely associated with acceptability and potential benefits of nuclear power. In contrast, persons with no interest in and knowledge of nuclear power would have risk perceptions related to their trust in nuclear operation and the government, which partially supported the ELM hypothesis. All these results indicated that the government in China plays an important role in rational risk perceptions, and well-designed communication of risks will help the public to be involved in risk management and improve people’s rational acceptance of risk.     

Development of Micropore Mufflers

Research on micropore mufflers was started in the middle of the 1970s. In this article we have summarized and systematized what has been done in the past 20 years. Some expressions, derivations, and calculation curves have been formally modified to make them easier to understand and apply. Some new considerations have been added. The main purpose of this article is to introduce the fundamentals, theories, and calculations that are useful for the design of a micropore muffler. Therefore, we have not included any experiments. Direct and indirect experimental proofs can be found in the given relevant references. Micropore mufflers are grouped into two classes, simple micropore mufflers and expansion micropore mufflers. The latter have additional noise reduction due to flow expansion. Noise reduction and flow are two important aspects that have been discussed in detail.     

基于交通冲突技术的交织区交通安全评价

为了分析城市快速路多个交织区之间的相对交通安全状况,及比选同一个交织区的多个设计方案,建立基于交通冲突技术的交织区交通安全评价方法。首先,使用微观交通仿真软件模拟一定道路交通条件下车流运行状态及其随时空变化的过程,并生成车辆轨迹文件;然后,运用交通安全替代评价模型(SSAM)分析车辆轨迹文件,识别和统计交通仿真过程中"发生"的交通冲突;最后,以交织区交通冲突数与交织区交通量、交织区长度的比值作为评价指标,评价交织区的交通安全状况。案例分析表明,交织区交通冲突率越高,其服务水平往往越低;交织区的交通冲突率有随交织区交通量增加而增加,随交织区长度、交织区车道数的增加而降低的趋势。     

宏观环境督察信息获取机制研究

本文对信息获取过程中的信息来源分析方法、信息采集方法、信息检索方法、信息加工方法进行了简要介绍,并将上述信息获取方法用于宏观环境督察信息获取过程中,研究了宏观环境督察信息获取机制,并对宏观环境督察信息获取过程进行了详细地分析,为后续的宏观环境督察信息发布提供了基础。     

石油污染对土壤生态的影响及修复技术分析

土壤修复现已逐渐成为环保领域新的热点。石油类污染物在土壤中迁移转化,使土壤的pH、有机质、含水率、生物群落结构等发生了改变。研究土壤理化性质、非生物因子和生物因子的动态关系是选择最佳修复技术的依据。介绍了目前主要的石油污染土壤修复技术及其优缺点和适用范围,提出理化一生物联用的原位修复技术是未来发展方向;石油污染物复杂多样,可通过构建复合高效菌群提高其生物修复效率。     

Modeling Monthly Fluctuations in Submersion Area of a Dammed River Reservoir: A Case Study1

Lu, Haorong, S. Samuel Li, and Jinsong Guo, 2012. Modeling Monthly Fluctuations in Submersion Area of a Dammed River Reservoir: A Case Study. Journal of the American Water Resources Association (JAWRA) 1‐13. DOI: 10.1111/jawr.12003 Abstract: Fluctuations in water submersion of the Three Gorges Reservoir in China have not been explored in spite of their important implications for shoreline erosion and other undesirable consequences. This article aims to quantify the monthly fluctuations in response to changing hydraulic parameters and regional climatic factors. Flow velocity and water levels distributed along the 609‐km long dammed river reservoir are calculated with a one‐dimensional hydrodynamics model. Evaporation of water from the surface of the reservoir is determined using mass transfer‐based methods. Calculated flow velocities and water levels compare well with field data. We show that the water surface slope decreases with rising water level at the dam, and decreases to almost zero during the winter months of water storage when the downstream water level reaches the normal pool level. The submersion area varies between 830 and 1,070 km² over the year or over 20% of the reservoir zone will experience the annual cycle of dry land and partial or complete submersion. These fluctuations are of relevance to shoreline management and to the prevention and restoration of shoreline erosion. Evaporation is estimated to fluctuate between 1,240 and 26,110 tons of water per month per kilometer length of reservoir channel; this can possibly affect the hydrological budget of the reservoir region. The simple methodologies discussed in this article can easily be applied to other dammed river reservoirs for submersion estimates.     

四川省开展环境权益交易的对策建议

2011年成都市获得四川省政府批复开始排污权交易的试点工作,这标志着四川省以市场化手段解决环境问题向前迈进一大步。排污权是典型的环境权益,因此本文主要以排污权交易为视角,借鉴各试点省成熟的经验,提出四川省开展环境权益交易的几点建议。     

西部旅游城市入境旅游流与饭店耦合协调度对比研究——以西安、成都为例

选取西安和成都两个西部典型旅游城市为样本,借鉴物理学耦合协调度函数,建立入境旅游流与星级饭店的耦合度模型和协调度模型,研究两城市入境旅游流与星级饭店的耦合状况,对其进行定量对比分析,探寻入境旅游需求与饭店供给的内在关系、耦合协调时序演变规律.研究发现,利用耦合协调度模型可以较好地评价两者的耦合协调状况;西安、成都的入境旅游流与星级饭店综合发展水平总体呈上升态势;两城市的入境旅游流与星级饭店系统协调度都经历了从失调到协调的演变过程,成都两系统的耦合协调度低于西安;大事件对于入境旅游和饭店业的发展都有明显的影响.