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851.
852.
Development of an ELISA for the detection of bromoxynil in water 总被引:4,自引:0,他引:4
For development of an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the nitrile herbicide bromoxynil, the polyclonal antibodies raised against 2,6-dibromo-4-cyano-phenoxyacetic acid (hapten) conjugated to bovine serum albumin (BSA) by the N-hydroxysuccinimide-activated ester method. Antiserum with a sufficiently high titer to provide the determinations of targeted compounds was obtained only 77 days after the primary immunization. Antiserum A2 was applied to the residual analysis of some water samples, under optimized ELISA condition, the quantitative working range was from 10 to 500 ppb with a limit of detection of 5 ppb. Cross-reactivity to structurally similar agrochemicals and related chemicals was determined. The antiserum showed little cross-reactivity with 2,6-dibromophenol and bromoxynil octanoate ester which have a dibromophenol group as common structure, but showed no cross-reactivity with other herbicides. Each water sample (river water, tap water, purified water, and bottled water) had a matrix effect and was investigated by adding Tween20 in the assay buffer. These four kinds of water samples were fortified with bromoxynil at several concentration levels and were directly analyzed with only dilution with an equal volume of antiserum solution, the mean recovery was 102.3%, and the mean coefficient of variation was 5.96%. The proposed ELISA turned out to be a powerful tool for monitoring of residual bromoxynil in water samples at trace level. 相似文献
853.
Analysis of chemical compositions contributable to chemical oxygen demand (COD) of oilfield produced water 总被引:1,自引:0,他引:1
This work was to give a comprehensive estimation for the chemical compositions contributable to COD of the produced water treatment system. For this purpose, the wastewater samples were collected from an onshore wastewater treatment plant. The chemical compositions of the wastewater were investigated, and the COD contributed by each component was estimated. The results showed that the COD levels of O&G and SS presented decreasing trends during the whole process and achieved total removal percentages of 95.1% and 62.3%, respectively. The final COD of organic acids and low-molecular-weight carbonyl compounds were respectively lowered to nearly 64% and 35% of their initial levels, and no regular trends were found for the COD of these chemicals during the whole treatment process. The COD of inorganic components presented minor variations at all sampling spots. The majority of COD was originated from O&G in raw wastewater. The COD contributed by O&G decreased greatly with continuous treatment and finally was lower than 17% of measured COD. At each sampling spot, the ratios of COD contributed by SS did not exceed 7.6% of measured COD. Other measured chemicals, including organic acids, carbonyl compounds, volatile phenols, reductive anions, metals and TDP were not the main sources of COD during the whole treatment process, and the ratio of COD was below 9% at each sampling spot. Most of the soluble components contributable to residual COD were still unknown after biological treatment, and the COD contributed by these components was greater than 57% of measured COD. 相似文献
854.
Shu HY Lu HC Fan HJ Chang MC Chen JC 《Journal of the Air & Waste Management Association (1995)》2006,56(6):852-858
In the past decade, the treatment amount of municipal solid waste (MSW) by incineration has increased significantly in Taiwan. By year 2008, approximately 70% of the total MSW generated will be incinerated. The energy content (usually expressed by lower heating value [LHV]) of MSW is an important parameter for the selection of incinerator capacity. In this work, wastes from 55 sampling sites, including villages, towns, cities, and remote islands in the Taiwan area, were sampled and analyzed once a season from April 2002 to March 2003 to determine the waste characteristics. The LHV of MSW in Taiwan was predicted by the multilayer perceptron (MLP) neural networks model using the input parameters of elemental analysis and dry- or wet-base physical compositions. Although all three of the models predicted LHV values rather accurately, the elemental analysis model provided the most accurate prediction of LHV values. Additionally, the wet-base physical composition model was the easiest and most economical. Therefore, the waste treatment operators can choose the more appropriate analysis method considering situations themselves, such as time, equipment, technology, and cost. 相似文献
855.
856.
Viable contribution of Tibetan sacred mountains in southwestern China to forest conservation 下载免费PDF全文
The Tibetan sacred mountains (TSMs) cover a large area and may represent a landscape‐scale conservation opportunity. We compared the conservation value of forests in these mountains with the conservation value of government‐established nature reserves and unmanaged open‐access areas in Danba County, southwestern China. We used Landsat satellite images to map forest cover and to estimate forest loss in 1974–1989, 1989–1999, and 1999–2013. The TSMs (n = 41) and nature reserves (n = 4) accounted for 21.6% and 29.7% of the county's land area, respectively. Remaining land was open‐access areas (i.e., areas without any restrictions on resource use) (56.2%) and farmlands (2.2%). Within the elevation range suitable for forests, forest cover did not differ significantly between nature reserves (58.8%) and open‐access areas (58.4%), but was significantly higher in TSMs (65.5%) after controlling for environmental factors such as aspect, slope, and elevation. The TSMs of great cultural importance had higher forest cover, but patrols by monastery staff were not necessarily associated with increased forest cover. The annual deforestation rate in nonsacred areas almost tripled in 1989–1999 (111.4 ha/year) relative to 1974–1989 (40.4 ha/year), whereas the rate in TSMs decreased in the later period (19.7 ha/year vs. 17.2 ha/year). The reduced forest loss in TSMs in 1989–1999 was possibly due to the renaissance of TSM worship and strengthened management by the local Buddhist community since late 1980s. The annual deforestation rate in Danba decreased dramatically to 4.4 ha/year in 1999–2013, which coincided with the implementation of a national ban on logging in 1998. As the only form of protected area across the Tibetan region during much of its history, TSMs have positively contributed to conserving forest at a landscape scale. Conservation of TSM forests largely relied on the strength of local religious institutions. Integrating community‐based conservation of TSMs within the government conservation network would benefit the conservation of the Tibetan region. 相似文献
857.
858.
859.
苯酚对两种微囊藻生长的作用效应 总被引:1,自引:0,他引:1
用不同浓度的苯酚处理无毒铜绿微囊藻和有毒铜绿微囊藻。结果显示不加苯酚处理时,有毒藻生长量明显高于无毒藻;100μg/mL的苯酚促进无毒藻生长而抑制有毒藻的生长;苯酚浓度增至200μg/mL,无毒藻的生长才受到抑制。高浓度苯酚胁迫下的藻细胞:光合速率大幅度降低;可溶性蛋白含量减少;细胞膜透性增大;超氧阴离子(O2^-)产生速率和丙二醛(MDA)相对含量升高;超氧化物歧化酶(SOD)活性降低。这些变化有毒藻较无毒藻更显著。表明:有毒微囊藻是水华中的优势种群;两种藻中有毒藻对苯酚的敏感性更大。 相似文献
860.
Summary. Phytotoxic activity of single and combined application of water soluble and volatile compounds of Cistus ladanifer on germination and early root growth of subterranean clover was investigated. Total germination, lag and speed of germination
were both inhibited and stimulated, with the activity of volatiles on total germination depending upon the presence of water
solubles. Antagonism between water solubles and volatiles was always found, resulting in a reduction of inhibition or a shift
from inhibition to stimulation. It is suggested that the simultaneous presence of water solubles and volatiles might result
in changes of the chemical nature of metabolites released by C. ladanifer. Early root growth was always inhibited but only by water solubles, and no interaction was found. The ecological implications
of these results are discussed in terms of the exhaustion of competitors seedbanks by a two-step process in which germination
is less inhibited or even stimulated by water solubles and volatiles, followed by a stronger and volatiles-independent inhibition
of early root growth.
Received 13 June 2001; accepted 13 Dezember 2001. 相似文献