Proposal for standardized prenatal ultrasound assessment of the fetus with congenital diaphragmatic hernia by the European reference network on rare inherited and congenital anomalies (ERNICA)

Congenital diaphragmatic hernia is a rare disease associated with high mortality and morbidity. Antenatal ultrasound screening identifies more than 70% of cases, providing the opportunity for in utero referral to a tertiary care center for expert assessment and perinatal management. Additional genetic and morphologic assessment may be used to rule out associated anomalies. In isolated cases, the outcome may be predicted prenatally by medical imaging. The combination of lung size and liver herniation is a widely accepted method to stratify fetuses into groups with an increasing degree of pulmonary hypoplasia and corresponding mortality rates. Ultrasound measurement of the observed to expected lung-to-head ratio (o/e LHR) is most widely used. The o/e LHR is an independent predictor of survival and short-term morbidity. Finally, evaluation of stomach position has recently been introduced as an indirect method to estimate severity of the disease in left-sided defects, as it has been shown to correlate with the proportion of intrathoracic liver. Herein, we propose a protocol for the standardized ultrasound assessment of fetuses with isolated CDH and individualized prediction of neonatal outcome.     

Aromatic Copolyester-based Nano-biocomposites: Elaboration, Structural Characterization and Properties

Biodegradable polymers are one of the most promising ways to replace non-degradable polymers. But, to be a real alternative to classical synthetic polymers and find applications, biopolymer (biodegradable polymer) properties have to be enhanced. Nano-biocomposites, which are obtained by incorporation of nanofillers into a biomatrix, are an interesting way to achieve these improvements. Modified and unmodified montmorillonites have been introduced into a biodegradable aromatic copolyester, poly(butylene adipate-co-terephthalate) (PBAT). Structural characterization, thermal and mechanical tests have been carried out to understand better the relations between the nanofillers structuring and the final nano-biocomposite properties. Main results show that clay incorporation and the obtained intercalated structures improve PBAT properties (enhanced thermal stability, increased stiffness) and thus may increase the attractiveness of this biopolymer.     

Prediction of the impact of logging activities on forest cover: a case-study in the east province of Cameroon

The objective of this study was to test the value of the concept of the net commercial value of standing timber in predicting the impact of logging activities on forest-cover modifications. A study area was selected in the East province of Cameroon which contains major primary forests and which contributes strongly to national timber production. A Geographic Information System containing ecological and economic variables was used in combination with remote sensing data to define the net commercial value of standing timber in the East province. Taking account of the potential commercial value of standing timber improves our understanding of the spatial determinants of logging activities and of the resulting forest-cover modifications. The occurrence of logging-induced forest-cover modifications increases with the value of forest rent. In one of the study sites, half of the very high rent areas have already been logged. In that site, therefore, it is mostly the low rent or marginal forest areas that remain unlogged. This was not the case, however, throughout the study area as shown by the observations at another site.     

Metacommunity structure of pond macroinvertebrates: effects of dispersal mode and generation time

A recent challenge in community ecology is to understand under what conditions local and regional processes may be more important in shaping community structure. We investigated the role of dispersal mode and generation time for communities of macroinvertebrates in two sets of connected ponds during three consecutive years. We found no evidence that generation time affected metacommunity structure, possibly because statistical power was limited because the range of generation times present was small. In contrast, we found that the spatial structure of the macroinvertebrate metacommunities differed with dispersal mode in one of two sets of ponds. Passive dispersers showed positive distance-dissimilarity correlations, suggesting mass effects via the pond connections. These correlations did not stretch beyond the first pond downstream suggesting that, in this chain of connected ponds, intervening ponds effectively buffered dispersal. Active dispersers did not show any consistent spatial pattern, possibly because intensive over-land dispersal homogenized the metacommunity. Thus, although pond connections probably equally promoted dispersal of actively and passively dispersing macroinvertebrates, the implications for the structure of their metacommunities may depend on their dispersal mode. We conclude that dispersal mode has the potential to affect the mechanisms that are integral to metacommunity structure.     

Extinction, recolonization, and dispersal through time in a planktonic crustacean

Dormant propagule banks are important reservoirs of biological and genetic diversity of local communities and populations and provide buffering mechanisms against extinction. Although dormant stages of various plant and animal species are known to remain viable for decades and even centuries, little is known about the effective influence of recolonization from such old sources on the genetic continuity of intermittent populations under natural conditions. Using recent and old dormant eggs recovered from a dated lake sediment core in Kenya, we traced the genetic composition of a local population of the planktonic crustacean Daphnia barbata through a sequence of extinction and recolonization events. This was combined with a phylogeographic and population-genetic survey of regional populations. Four successive populations, fully separated in time, inhabited Lake Naivasha from ca. 1330 to 1570 AD, from ca. 1610 to 1720 AD, from ca. 1840 to 1940 AD, and from 1995 to the present (2001 AD). Our results strongly indicate genetic continuity between the 1840-1940 and 1995-2001 populations, which are separated in time by at least 50 years, and close genetic relatedness of them both to the 1330-1580 population. A software tool (Colonize) was developed to find the most likely source population of the refounded 1995-2001 population and to test the number of colonists involved in the recolonization event. The results confirmed that the 1995-2001 population most probably developed out of a limited number of surviving local dormant eggs from the previous population, rather than out of individuals from regional (central and southern Kenya) or more distant (Ethiopia, Zimbabwe) populations that may have immigrated to Lake Naivasha through passive dispersal. These results emphasize the importance of prolonged dormancy for the natural long-term dynamics of crustacean zooplankton in fluctuating environments and suggest an important role of old local dormant egg banks in aquatic habitat restoration.