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91.
92.
Infestation by leeches on adult freshwater turtles Phrynops geoffroanus in two areas of the Uberabinha River, in Uberlandia, was investigated during the summer (January 2000). Fifty eight turtles were trapped: 26 (12 males, 14 females) in an agricultural area and 32 (16 males, 16 females) in an area with a more urban environment. The females present larger length and body mass (291.77+/-29.18 mm; 2233.30+/-511.40 g) than males (259.71+/-33.15 mm; 1488.68+/-529.35 g). Blood samples were drawn from the retrorbital sinus using heparinized micro-hematocrit capillary tubes, for microscopic and direct examination for intracellular parasites. Ectoparasitism by leeches, Placobdella bistriata, on young and adult animals was observed in the limb cavities of 28.1% of the urban area turtles. No leeches were found on the turtles in the agricultural area. Hemogregarine erythrocytic gametocytes were found in 15.4% of the agricultural area turtles, and in 37.5% of the urban area turtles. Concurrent parasitism by leeches and hemogregarines on the same individuals was seen on six turtles. Infections with microorganisms (bacteria and fungus) facilitated by the bites of leeches were not observed in another study on the same sample of infested turtles. However, 19% of the turtles collected in the agricultural area and 15% of the turtles from the urban area presented normocytic acute anemia. The occupation of the soil apparently interfered in the aquatic ecosystem favoring parasitism in the urban area. 相似文献
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94.
Andrea Battistone Gabriele Buttinelli Paolo Bonomo Stefano Fiore Concetta Amato Pietro Mercurio Antonella Cicala Josef Simeoni Adelheid Foppa Maria Triassi Francesca Pennino Lucia Fiore 《Food and environmental virology》2014,6(1):13-22
This study evaluated the presence and seasonal distribution of polio and other enteroviruses in four wastewater treatment plants in three cities in Italy, using different treatment systems. Detection of enteroviruses was carried out by virus isolation in cell cultures after concentration of water samples collected at both inlet and outlet of the treatment plants, following the methods described in the WHO guidelines. Viral serotypes isolated before and after water treatment were compared. Forty-eight non-polio enteroviruses were isolated from 312 samples collected at the inlet of the four wastewater treatment plants, 35 of which were Coxsackievirus type B (72.9 %) and 13 Echovirus (27.1 %). After treatment, 2 CVB3, 1 CVB5, and 1 Echo 6 were isolated. CVB3 and Echo 6 serotypes were also detected in samples collected at the inlet of the TP, in the same month and year. The high rate of detection of infectious enteroviruses in inlet sewage samples (30.1 %) indicates wide diffusion of these viruses in the populations linked to the collectors. The incomplete removal of infectious viruses following sewage treatment highlights possible risks for public health relate to treated waters discharge into the environment. 相似文献
95.
Seasonal differences in the locomotor activity pattern of Podarcis sicula held in constant conditions in the laboratory were found to be associated with systematic differences in both the freerunning period of locomotor rhythms () and the circadian activity time (). In order to establish whether the pineal played a role in the control of seasonal changes in circadian parameters, we examined the effects of pinealectomy in constant conditions on the locomotor behavior of lizards displaying the bimodal activity pattern typical of summer. In most lizards pinealectomy lengthened , shortened and abolished the bimodal activity pattern. Pinealectomy induced a sudden transition from the typical locomotor behavior of summer, characterized by a marked bimodal pattern, short and long , to the typical locomotor behavior of autumn, characterized by a unimodal pattern, a long and short . These results demonstrate that the pineal plays a central role in the seasonal reorganization of the circadian system that occurs in P. sicula. 相似文献
96.
Lucia Barra Maria Valeria Ruggiero Jixin Chen Wiebe H. C. F. Kooistra 《Environmental science and pollution research international》2014,21(1):548-557
In the scope of the development of a microarray PhyloChip for the detection of toxic phytoplankton species, we designed a large series of probes specific against targets in the nuclear large subunit (LSU) rRNA of a range of Pseudo-nitzschia species and spotted these onto the microarray. Hybridisation with rRNA extracted from monoclonal cultures and from plankton samples revealed many cross-reactions. In the present work, we tested the functionality and specificity of 23 of these probes designed against ten of the species, using a dot-blot procedure. In this case, probe specificity is tested against the target region in PCR products of the LSU rRNA gene marker region blotted on nitrocellulose filters. Each filter was incubated with a species-specific oligoprobe. Eleven of the tested probes showed specific responses, identifying seven Pseudo-nitzschia species. The other probes showed non-specific responses or did not respond at all. Results of dot-blot hybridisations are more specific than those obtained with the microarray approach and the possible reasons for this are discussed. 相似文献
97.
Camila Maia-Silva Michael Hrncir Dirk Koedam Renato Jose Pires Machado Vera Lucia Imperatriz-Fonseca 《Die Naturwissenschaften》2013,100(1):101-105
Between April and June of 2012 mantisflies (Plega hagenella) were found to be extensively parasitizing the nests of two groups of managed colonzies of eusocial stingless bees (Melipona subnitida) in the semi-arid region of northeastern Brazil. The mantisfly larvae developed inside closed brood cells of the bee comb, where each mantispid larva fed on the bee larva or pupa present in a single brood cell. Mature mantispid larvae pupated inside silken cocoons spun in place within their hosts' brood cells then emerged as pharate adults inside the bee colony. Pharate adults were never attacked and killed by host colony workers. Instead, colony workers picked up the pharates and removed them from the nest unharmed, treating them similar to the way that the general refuse is removed from the nest. Adult mantispids subsequently eclosed from their pupal exuviae outside the nest. Manipulative experiments showed that post-eclosion adult mantispids placed back within active bee colonies were quickly attacked and killed. These observations demonstrate that pharate and post-eclosion adults of P. hagenella are perceived differently by colony workers and that delayed adult eclosion is an important functional element in the parasitic life strategy of P. hagenella, allowing adults to escape without injury from the bee colonies they parasitize. 相似文献
98.
Pavoni Enrico Bertasi Barbara Galuppini Elisa Mangeri Lucia Meletti Francesca Tilola Michela Carta Valentina Todeschi Silvia Losio Marina-Nadia 《Food and environmental virology》2022,14(1):69-76
Food and Environmental Virology - To observe the prevalence of contamination by hepatitis A virus (HAV) and norovirus (NoV) in different food types, 9242 samples were analyzed over a 6-year period... 相似文献
99.
100.
Viñas L Franco A Blanco X Bargiela J Soriano JA Perez-Fernandez B Gonzalez JJ 《Environmental science and pollution research international》2012,19(2):529-539
The aim of this study was to establish the temporal trends and spatial distribution of Polycyclic Aromatic Hydrocarbons (PAHs)
in Ria de Vigo by means of studying the PAH burden in wild mussels. The samples were collected in eight sites along the margins
of the Ria de Vigo during November from 1998 to 2008 and Polycyclic Aromatic Hydrocarbon contents were determined using high
performance liquid chromatography–fluorescence derivatization (HPLC–FLD). Quality of chemical analysis was guaranteed by participation
in QUASIMEME intercalibration exercises carried out during the period of analysis. The concentrations for the sum of 13 PAHs
were in the range 24–480 μg/kg dw, typical of an urban and industrialized area (50–500 μg/kg dw) except for a punctual input
in La Guía in 1998 that led to a concentration above 1,000 μg/kg dw. In general, the sites in the outermost section of the
estuary (ría) showed the lowest values, with an increasing gradient in the medium and inner parts of the ría. Temporal patterns
show, in general, a downward trend. The relative proportion of 3 and 4 ring PAHs also varies with the site, the former being
predominant in the outermost area and the latter in the medium and inner parts of the ría. This is consistently related with
the sources and processes taking place in different areas of the estuary. 相似文献