Concentration statistics for mixing-controlled reactive transport in random heterogeneous media

Uncertainty in the distribution of hydraulic parameters leads to uncertainty in flow and reactive transport. Traditional stochastic analysis of solute transport in heterogeneous media has focused on the ensemble mean of conservative-tracer concentration. Studies in the past years have shown that the mean concentration often is associated with a high variance. Because the range of possible concentration values is bounded, a high variance implies high probability weights on the extreme values. In certain cases of mixing-controlled reactive transport, concentrations of conservative tracers, denoted mixing ratios, can be mapped to those of constituents that react with each other upon mixing. This facilitates mapping entire statistical distributions from mixing ratios to reactive-constituent concentrations. In perturbative approximations, only the mean and variance of the mixing-ratio distribution are used. We demonstrate that the second-order perturbative approximation leads to erroneous or even physically impossible estimates of mean reactive-constituent concentrations when the variance of the mixing ratio is high and the relationship between the mixing ratio and the reactive-constituent concentrations strongly deviates from a quadratic function. The latter might be the case in biokinetic reactions or in equilibrium reactions with small equilibrium constant in comparison to the range of reactive-constituent concentrations. When only the mean and variance of the mixing ratio is known, we recommend assuming a distribution that meets the known bounds of the mixing ratio, such as the beta distribution, and mapping the assumed distribution of the mixing ratio to the distributions of the reactive constituents.     

Use of sulfadimethoxine in intensive calf farming: evaluation of transfer to stable manure and soil

After prophylactic treatment of 50 calves with 62mgkg(-1)day(-1) of sulfadimethoxine (SDM) for five days, the levels of the drug over time were followed in feces, bedding and stable manure, and then in the soil of a manured field and surrounding drainage courses. Analysis were done by HPLC after applying to the different matrices a quick and simple extraction procedure. The half-life of the drug in bedding was very short (24h). In stable manure the degradation rate of the drug slowed down and the calculated half-life was 64 days, with 390microgkg(-1) of SDM still detectable after three months maturation. However, in a five months matured stable manure obtained from other groups of calves subjected to the same prophylactic treatment, levels of SDM were <50muicrokg(-1) (LOD of the analytical method). After field fertilization with this manure, no traces of SDM were found in soil (LOD 10microgkg(-1)) or in the water (LOD 2microgl(-1)) from the surrounding drainage courses. Using the internationally recognised DAPHTOXKIT-Ftrade mark, a SDM toxicity test toward Daphnia magna was performed in the range 10-100mgl(-1). The test gave negative results both after 24 and 48h, confirming that microcrustaceans are less sensitive than other models to the toxicity of antibacterials. However, based on data from other authors, concerning algal toxicity and microbial inhibition, and on the analytical results from the current field study, the calculated worst-case EC50/PEC ratio for SDM both in freshwater and in soil was still >1000.     

Nonpoint-Source Agricultural Hazard Index: A Case Study of the Province of Cremona, Italy

This paper reports the results of a study aimed at the evaluation of the hazard level of farming activities in the province of Cremona, Italy, with particular reference to groundwater. The applied methodology employs a parametric approach based on the definition of potential hazard indexes (nonpoint-source agricultural hazard indexes, NPSAHI). Two categories of parameters were considered: the hazard factors (HF), which represent all farming activities that cause or might cause an impact on groundwater (use of fertilizers and pesticides, application of livestock and poultry manure, food industry wastewater, and urban sludge), and the control factors (CF), which adapt the hazard factor to the characteristics of the site (geographical location, slope, agronomic practices, and type of irrigation). The hazard index (HI) can be calculated multiplying the hazard factors by the control factors and, finally, the NPSAHI are obtained dividing HI into classes on a percentile basis using a scale ranging from 1 to 10. Organization, processing, and display of all data layers were performed using the geographical information system (GIS) ArcView and its Spatial Analyst extension. Results show that the potential hazard of groundwater pollution by farming activities in the province of Cremona falls mainly in the fifth class (very low hazard).     

The Arctic sea butterfly<Emphasis Type="Italic"> Limacina helicina</Emphasis>: lipids and life strategy

The sea butterfly Limacina helicina was collected from May to September 2001 in Kongsfjorden, Spitsbergen, to investigate population structure and body and lipid composition with regard to life cycle and reproductive strategy. Veligers and juveniles were only found in late autumn and spring, whereas females occurred from July to September. The size of the females increased until mid-August and decreased in September. Dry and lipid mass were closely related to size; dry mass increased exponentially and lipids linearly with size. The lipid content was highest in veligers (31.5% of dry mass) and juveniles (23.6%) but low in females (<10%). Phospholipids were the dominating lipid class followed by triacylglycerols. Females, veligers, and egg ribbons, all from September, were richest in phospholipids. Juveniles contained the highest amounts of triacylglycerols, whereas females had low levels in July and the beginning of August. In mid-August, levels of triacylglycerols were variable and higher. This suggests that females were in their main spawning period and the high variability in triacylglycerols points to different stages within the spawning cycle. Enhanced amounts of free fatty acids in females from July may be related to gonad development. The 16:1(n-7) fatty acid was more dominant in spring whereas 18:4(n-3) increased in summer and autumn, which reflects a change in diet from diatom-dominated food items in spring to dinoflagellates in summer/autumn. Small amounts of long-chain monounsaturated fatty acids suggest ingestion of copepods, and the fatty acid composition of veligers feeding on particulate matter. L. helicina has a one-year life cycle with peak spawning in August and over-winters as veligers that may grow to juveniles during the winter period. They metamorphose into juveniles during spring, develop to males in early summer, and mature into females in July and August.Communicated by M. Kühl, Helsingør     

A soil alteration index based on phospholipid fatty acids

Phospholipid fatty acid (PLFA) analysis has gained great importance in the study of soil microbial community structure. This structure can give indication of the soil status. Purpose of the present paper is to analyse PLFA patterns in altered agricultural soils in order to develop a soil status alteration index. Soils subjected either to intensive agricultural exploitation, or to overflow by municipal and industrial wastes, or to irrigation with saline waters were analysed for PLFA content and compared to adjacent untreated soils by means of different statistical techniques.

Principal component analysis separated PLFAs in three groups: unsaturated PLFAs (first axis, 48% of total variance), monounsaturated and cyclopropane PLFAs (second axis, 28% of total variance) and polyunsaturated PLFAs (third axis, 24% of total variance).

By means of canonical discriminant analysis, a soil alteration index (SAI) was produced from 15 PLFAs using two data sets. A third data set was used to test the SAI general validity together with other data sets reported in literature. The index validity was confirmed in most cases: SAI gave higher scores for control soils and was generally able to classify soils according to their reported degree of alteration.     

Trade and recycling of used tyres in Western and Eastern Europe

Truck tyres can cause significant environmental pressure through the life cycle. The main aim of this paper is investigate to what extent international policy measures on foreign trade, international recycling and harmonisation of legislation can contribute in effectively reducing environmental pressure caused in the truck tyre life cycle. A two-region simulation model, representing Western and Eastern Europe, is developed that integrates the complete life cycle, incorporates environmental impacts in its economic analysis, is technically dynamic by accounting for learning-by-doing effects, and allows for variations in trade of new and old truck tyres. In this study the economic, environmental and social effectiveness of harmonisation and trade measures in the European life cycle for truck tyre is tested. Several conclusions can be drawn from the model simulations. First, the environmental effects caused by the trade of used tyres from Western to Eastern Europe are of limited impact on the overall environmental damage caused by truck tyres. The consumption stage is by far the main contributor to environmental damage. Within the marginal analysis of trade, harmonisation of disposal fees illustrated to generate very limited positive results. The private and external costs in the solid waste management (SWM) stage are too limited to have a notable impact on the overall configuration of the European tyre life cycle. The introduction of strict laws on tread depth in Eastern Europe has a much stronger impact on material flows than the harmonisation scenario. This suggests that domestic policy measures should be the primarily focus on interventions in this stage of the life cycle, for instance, by improving the management of tyre pressure. Because trade of used tyres has little impact on the consumption stage, this issue should not get priority in European environmental programs.     

Rhythmic vertical migration of the gastropod Cerithidea decollata in a Kenyan mangrove forest

In Mida Creek, Kenya (3°20′S, 40°5′E), at high water, the snail Cerithidea decollata dwells on the trunks of mangrove trees (Avicennia marina), while during low water it migrates to the ground, foraging at various distances from the trunk, where it aggregates again well before the incoming tide. Snails from the upper shore level are 150–200 m distant from those living at the lower shore level and they cluster at lower heights on trunks. In any case, sufficient height is usually attained to avoid being submersed. An experiment was designed (February and October 2005), exchanging individuals from different shore levels subject to different tide regimes, in order to test whether snails rely on internal information or on external, direct cues, to adapt their behaviour to local conditions. Results show that C. decollata mostly rely on internal information, presumably based on an internal clock. When individuals from upper and lower shore levels were exchanged, their internal clocks continued to govern when to ascend the home trunk and how high to climb for five to six successive tides, after which the behaviour was reset to the new local conditions.     

UVR-induced photoinhibition of summer marine phytoplankton communities from Patagonia

During austral summer 2006, experiments were carried out to evaluate the effects of ultraviolet radiation (UVR, 280–400 nm) on carbon fixation of natural phytoplankton assemblages from Patagonia (Argentina). Surface water samples were collected (ca. 100 m offshore) at mid morning using an acid-cleaned (1 N HCl) dark container. The short-term impact of UVR (measured as radiocarbon incorporation) was immediately assessed by exposing samples to three artificial illumination treatments: PAR (400–700 nm), PAR + UV-A (320–700 nm), and PAR + UV-A + UV-B (280–700 nm). Pico-nanoplankton characterized the assemblages, and taxon-specific pigment fingerprinting combined with CHEMTAX and supplemented with microscopic observations showed varied proportions of diatoms, chlorophytes, and cyanobacteria throughout January–February 2006. Photosynthetic efficiency, as assessed through assimilation numbers, was high [between 4.4 and 10.4 μg C (μg chl-a)⁻¹ h⁻¹], and it was probably favored by the supply of inorganic nutrients from the Chubut River. UVR-induced photoinhibition appeared to be related to the taxonomic composition: in general, higher photoinhibition was observed when diatoms dominated, whereas this was lower when samples were dominated by chlorophytes. Our data suggest that xanthophyll pigments might have provided only limited protection in these already highlighted acclimated assemblages.     

Mass balanced and dynamic simulations of trophic models of kelp ecosystems near the Mejillones Peninsula of northern Chile (SE Pacific): Comparative network structure and assessment of harvest strategies

Mass balanced trophic models for kelp ecosystems which include subsystems dominated by Macrocystis integrifolia, Lessonia trabeculata and areas of barren ground (BG) were constructed for subtidal areas near the Mejillones Peninsula (SE Pacific), Chile. Information on biomass, P/B ratios, catches, food spectrum, consumption and dynamics of commercial and non-commercial populations was obtained and examined using Ecopath with Ecosim software analyses. The biomass of blades of L. trabeculata and M. integrifolia represented the compartments most relevant to the subsystems studied. Within the herbivores, the sea urchin Tetrapigus niger was dominant, followed by the snails Turritella sp. and Tegula sp. The fishes Pinguipes chilensis and Cheilodactylus variegatus were the dominant predators, followed by the asteroids Heliaster helianthus and Meyenaster gelatinosus. The highest system throughput (72,512 g wet weight m⁻² year⁻¹) was calculated for the subsystem dominated by M. integrifolia. The mean trophic level of the catch ranged from 1.1 (subsystem dominated by L. trabeculata) to 1.3 (subsystem dominated by M. integrifolia) to 3.2 (barren ground subsystem), showing that harvesting in each system was concentrated either on primary producers (blades of kelp species) or top predator fishes. Although the values for the Relative Ascendency (A/C) fluctuated from 36.5 to 45%, suggesting that all the systems were immature, the subsystem dominated by M. integrifolia emerged as the least resistant to external disturbances (e.g. fisheries). This result agreed with the high value of the system recovery time (SRT) for the M. integrifolia subsystem as a response to combined fisheries scenarios. The results obtained using mixed trophic impact (MTI) and Ecosim [increasing the fishing mortality F_i by 4×] showed that in most of the cases the predictions had the same qualitative tendencies. One of the most important results obtained in this study was that exploitation of kelp blades as an alternative strategy to harvesting the whole plants appeared to be ecologically sustainable, since harvesting the blades propagated only small effects on the entire subsystem. The fish P. chilensis may be considered as a top predator species with a strong top-down control since an increase in its fishing mortality in the subsystem dominated by M. integrifolia produced a high SRT value, and the F_MSY was less than the originally entered F_i in Ecopath. Based on the results obtained, it was concluded that the trophic mass balanced models and simulated management scenarios offered good possibilities for the planning of interventions and manipulations or the planning of more sustainable management strategies in highly disturbed natural systems.     

Contribution of fishery discards to the diet of the Black-browed albatross (Thalassarche melanophris) during the non-breeding season: an assessment through stable isotope analysis

Black-browed albatrosses (Thalassarche melanophris) disperse over the Argentinean Continental Shelf and neighboring waters during their non-breeding season. It is one of the most frequent seabirds attending fishing vessels and also the most common Procellariform in the bycatch of longliners and trawlers in the area. Understanding the use of fishery discards by this species is an important issue when assessing the potential effect of strategic discard management in decreasing the abundance, interactions, and mitigating mortality. In the present study, we analyzed carbon and nitrogen stable isotope compositions in the blood of Black-browed albatrosses to assess the relative contribution of discards from different fisheries to the diet of this species in winter. Samples were obtained in winter 2011 from fishing vessels operating between 41–43°S and 57–59°W. No sex differences in δ¹³C and δ¹⁵N were observed. Results indicate that during their non-breeding season, isotopic signatures of Black-browed albatrosses are closer to discards and offal generated by fisheries and in particular by trawlers. The large fishing effort of trawl fisheries in Argentina highlights the urgency of an exhaustive analysis to find practical and effective ways to reduce the number of seabirds attending trawlers.