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91.
de Moura Stéfany G. Dauzakier Ligiane C. L. Pereira Leydiane O. Ramalho Teodorico C. de Oliveira Luiz C. A. Magalhães Fabiano 《Environmental science and pollution research international》2021,28(31):42093-42106
Environmental Science and Pollution Research - Water contamination is a common problem, especially considering dyes and drugs disposal. A possible and effective treatment method to remove these... 相似文献
92.
93.
Simone Guadagnucci Morillo Adriana Luchs Audrey Cilli Cibele Daniel Ribeiro Rita de Cássia Compagnoli Carmona Maria do Carmo Sampaio Tavares Timenetsky 《Food and environmental virology》2017,9(2):142-148
Norovirus (NoV) is recognized as the most common cause of foodborne outbreaks. In 2014, an outbreak of acute gastroenteritis occurred on a cruise ship in Brazil, and NoV became the suspected etiology. Here we present the molecular identification of the NoV strains and the use of sequence analysis to determine modes of virus transmission. Food (cream cheese, tuna salad, grilled fish, orange mousse, and vegetables soup) and clinical samples were analyzed by ELISA, conventional RT-PCR, qRT-PCR, and sequencing. Genogroup GII NoV was identified by ELISA and conventional RT-PCR in fecal samples from 5 of 12 patients tested (41.7%), and in the orange mousse food sample by conventional RT-PCR and qRT-PCR. Two fecal GII NoV samples and the orange mousse GII NoV sample were successfully genotyped as GII.Pe (ORF 1), revealed 98.0–98.8% identities among them, and shared phylogenetically distinct cluster. Establishing the source of a NoV outbreak can be a challenging task. In this report, the molecular analysis of the partial RdRp NoV gene provided a powerful tool for genotyping (GII.Pe) and tracking of outbreak-related samples. In addition, the same fast and simple extraction methods applied to clinical samples could be successfully used for complex food matrices, and have the potential to be introduced in routine laboratories for screening foods for presence of NoV. 相似文献
94.
95.
A comprehensive review of climate adaptation in the United States: more than before, but less than needed 总被引:2,自引:0,他引:2
Rosina Bierbaum Joel B. Smith Arthur Lee Maria Blair Lynne Carter F. Stuart Chapin III Paul Fleming Susan Ruffo Missy Stults Shannon McNeeley Emily Wasley Laura Verduzco 《Mitigation and Adaptation Strategies for Global Change》2013,18(3):361-406
We reviewed existing and planned adaptation activities of federal, tribal, state, and local governments and the private sector in the United States (U.S.) to understand what types of adaptation activities are underway across different sectors and scales throughout the country. Primary sources of review included material officially submitted for consideration in the upcoming 2013 U.S. National Climate Assessment and supplemental peer-reviewed and grey literature. Although substantial adaptation planning is occurring in various sectors, levels of government, and the private sector, few measures have been implemented and even fewer have been evaluated. Most adaptation actions to date appear to be incremental changes, not the transformational changes that may be needed in certain cases to adapt to significant changes in climate. While there appear to be no one-size-fits-all adaptations, there are similarities in approaches across scales and sectors, including mainstreaming climate considerations into existing policies and plans, and pursuing no- and low-regrets strategies. Despite the positive momentum in recent years, barriers to implementation still impede action in all sectors and across scales. The most significant barriers include lack of funding, policy and institutional constraints, and difficulty in anticipating climate change given the current state of information on change. However, the practice of adaptation can advance through learning by doing, stakeholder engagements (including “listening sessions”), and sharing of best practices. Efforts to advance adaptation across the U.S. and globally will necessitate the reduction or elimination of barriers, the enhancement of information and best practice sharing mechanisms, and the creation of comprehensive adaptation evaluation metrics. 相似文献
96.
Maria Rönnqvist Thedi Ziegler Carl-Henrik von Bonsdorff Leena Maunula 《Food and environmental virology》2012,4(1):26-33
Recent events have shown that humans may become infected with some pathogenic avian influenza A viruses (AIV). Since soil
and water, including lakes, rivers, and seashores, may be contaminated by AIV excreted by birds, effective methods are needed
for monitoring water for emerging viruses. Combining water filtration with molecular methods such as PCR is a fast and effective
way for detecting viruses. The objective of this study was to apply a convenient method for the detection of AIV in natural
water samples. Distilled water and lake, river, and seawater were artificially contaminated with AIV (H5N3) and passed through
a filter system. AIV was detected from filter membrane by real-time RT-PCR. The performance of Zetapor, SMWP, and Sartobind
D5F membranes in recovering influenza viruses was first evaluated using contaminated distilled water. SWMP, which gave the
highest virus recoveries, was then compared with a pre-filter combined GF/F filter membrane in a trial using natural water
samples. In this study, the cellulose membrane SMWP was found to be practical for recovery of AIVs in water. Viral yields
varied between 62.1 and 65.9% in distilled water and between 1 and 16.7% in natural water samples. The borosilicate glass
membrane GF/F combined with pre-filter was also feasible in filtering natural water samples with viral yields from 1.98 to
7.33%. The methods described can be used for monitoring fresh and seawater samples for the presence of AIV and to determine
the source of AIV transmission in an outbreak situation. 相似文献
97.
98.
Power matters in closing the phenotyping gap 总被引:1,自引:0,他引:1
Meyer CW Elvert R Scherag A Ehrhardt N Gailus-Durner V Fuchs H Schäfer H Hrabé de Angelis M Heldmaier G Klingenspor M 《Die Naturwissenschaften》2007,94(5):401-406
Much of our understanding of physiology and metabolism is derived from investigating mouse mutants and transgenic mice, and
open-access platforms for standardized mouse phenotyping such as the German Mouse Clinic (GMC) are currently viewed as one
powerful tool for identifying novel gene-function relationships. Phenotyping or phenotypic screening involves the comparison of wild-type control mice with their mutant or transgenic littermates. In our study, we explored
the extent to which standardized phenotyping will succeed in detecting biologically relevant phenotypic differences in mice
generated and provided by different collaborators. We analyzed quantitative metabolic data (body mass, energy intake, and
energy metabolized) collected at the GMC under the current workflow, and used them for statistical power considerations. Our
results demonstrate that there is substantial variability in these parameters among lines of wild-type C57BL/6 (B6) mice from
different sources. Given this variable background noise in mice that serve as controls, subtle phenotypes in mutant or transgenic
littermates may be overlooked. Furthermore, a phenotype observed in one cohort of a mutant line may not be reproducible (to
the same extent) in mice coming from a different environment or supplier. In the light of these constraints, we encourage
researchers to incorporate information on intrastrain variability into future study planning, or to perform advanced hierarchical
analyses. Both will ultimately improve the detectability of novel phenotypes by phenotypic screening.
Carola W. Meyer and Ralf Elvert contributed equally to this work. 相似文献
99.
Host selection and infection strategies of parasitoids often correlate with high parental investment and low numbers of progeny.
In this study, we investigate how additional internal mechanisms might shape brood size and fitness of the offspring. Emblemasoma auditrix is a parasitoid fly in which about 38 larvae hatch simultaneously in utero. After host location, a single larva is deposited
into the host, where it rapidly develops and pupates after about 5 days. The search for hosts can take several weeks, and
during that time, the larvae arrest their development and remain in the first larval instar. Nevertheless, the larvae increase
in weight within the uterus, and this growth correlates to a decrease in the number of larvae, although no larvae are deposited.
Thus, our data indicate a first case of prenatal cannibalism in an invertebrate with larvae feeding on each other within the
uterus of the adult. 相似文献
100.
Recruitment precision, i.e. the proportion of recruits that reach an advertised food source, is a crucial adaptation of social
bees to their environment. Studies with honeybees showed that recruitment precision is not a fixed feature, but it may be
enhanced by factors like experience and distance. However, little is known regarding the recruitment precision of stingless
bees. Hence, in this study, we examined the effects of experience and spatial distance on the precision of the food communication
system of the stingless bee Scaptotrigona mexicana. We conducted the experiments by training bees to a three-dimensional artificial patch at several distances from the colony.
We recorded the choices of individual recruited foragers, either being newcomers (foragers without experience with the advertised
food source) or experienced (foragers that had previously visited the feeder). We found that the average precision of newcomers
(95.6 ± 2.61%) was significantly higher than that of experienced bees (80.2 ± 1.12%). While this might seem counter-intuitive
on first sight, this “loss” of precision can be explained by the tendency of experienced recruits to explore nearby areas
to find new rewarding food sources after they had initially learned the exact location of the food source. Increasing the
distance from the colony had no significant effect on the precision of the foraging bees. Thus, our data show that experience,
but not the distance of the food source, affected the patch precision of S. mexicana foragers. 相似文献