Partitioning tracer tests as a remediation metric: Case study at naval amphibious base little creek,Virginia Beach,Virginia

The partitioning tracer test (PTT) is a characterization tool that can be used to quantify the porespace saturation (S_N) and spatial distribution of dense nonaqueous phase liquids (DNAPLs) in the subsurface. Because the method essentially eliminates data interpolation errors by directly measuring a relatively large subsurface volume, it offers significant promise as a remediation metric for DNAPL‐zone remediation efforts. This article presents, in detail, the design and results of field PTTs conducted before and after a DNAPL‐zone treatment at the Naval Amphibious Base Little Creek, Virginia Beach, Virginia. The results from different tracers yield a relatively large range in S_N estimates, indicating notable uncertainty and presenting significant challenges for meaningful interpretation. Several potential interpretation methods are presented, resulting in an estimated DNAPL removal range of 15 to 109 L. While this range is large, it is consistent with the DNAPL removal (～30 L) determined from analysis of effluent concentration measurements collected during the remediation efforts. At this site, the initial and final S_N values are low, and the relatively inconsistent performance of the various tracers indicates that these levels are near the lower practical quantification limit for these PTTs; however, the effective lower quantification limit for these tests is unknown. Generally, an understanding of lower quantification limits is particularly important for interpretation of post‐remediation PTTs because S_N values are likely to be low (due to remediation efforts) and the S_N estimated from the PTT may be used to predict long‐term dissolved plume behavior and assess associated environmental risk. Partitioning tracer test quantification limits are test‐specific, as they are dependent on a variety of factors including analytical uncertainty, tracer breakthrough characteristics, and tracer data integration techniques. The results of this case study indicate that methods for estimating lower quantification limits for field PTTs require further development. © 2004 Wiley Periodicals, Inc.     

High copper concentrations in squid livers in association with elevated levels of silver,cadmium, and zinc

Livers from 43 Loligo opalescens, 14 Ommastrephes bartrami, and 7 Symplectoteuthis oualaniensis were analyzed for their silver, cadmium, copper, zinc and iron contents. Copper concentrations of up to 15,000 μg/g dry weight were found in L. opalescens in conjunction with significant correlations between this element and Ag, Cd and Zn. The latter elements are known to affect Cu metabolism in terrestrial organisms; however, whether the correlations occurring in marine organisms represent casual, or cause and effect, relationships is as yet unknown.     

Testosterone stress does not increase asymmetry of a hormonally mediated sexual ornament in a lizard

The theory that fluctuating asymmetry is sensitive to both environmental and genetic stress is gaining acceptance among evolutionary biologists. Most empirical work has focused on ornamental traits on the assumption that they are more susceptible to stress. In tegumentary coloration is a common ornament in nature, and frequently has a hormonal basis. Earlier studies in the lizard Psammodromus algirus indicate that testosterone induces the development of head nuptial coloration in large males and, at the same time, produces an increase of the ectoparasite load and higher mortality. Hence, the manipulation of testosterone levels may be a way to increase ornament expression and simultaneously create conditions that may make symmetrical development difficult. This positive covariation between character size and symmetry is opposite to that expected in theory for sexually selected traits, so the predicted elevation of asymmetry due to the treatment cannot be confounded by any intrinsic association between symmetry and character size. We firstly consider the effect that testosterone supplementation has on two variables that reflect the symmetry of bilateral throat nuptial coloration in large P. algirus males. Also, we examine whether ornament symmetry is positively associated with reproductive success, a prediction of theory of sexually selected symmetry. Testosterone treatment did not increase the fluctuating asymmetry of throat coloration. Size asymmetry increased with character size in individuals with a fragmented colour pattern, but changed suddenly to a highly symmetric pattern in individuals with non-fragmented coloration. Mirror asymmetry decreased steadily with character size. These results suggest that the development of coloration on both sides of the throat midline follows a random pattern. Asymmetry did not correlate with variables that estimated reproductive success, suggesting that asymmetry is not affected by physiological stress and that this trait is not a sexually selected signal in P. algirus. Received: 1 July 1996 / Accepted after revision: 4 May 1997     

Game theory, multi-modal signalling and the evolution of communication

We discuss how the theoretical framework related to selection pressures on multi-component and multi-modal signalling introduced by the article of Wilson et al. in this special issue could usefully be built both theoretically and empirically. Theoretically, we suggest that the game theory approach of Wilson et al. could be generalised using the methodology of adaptive dynamics in order to indicate evolutionary trajectories and paths of evolution. This might indicate the relative likelihood of finding different equilibriums empirically in situations where the game theory explorations of Wilson et al. suggest that multiple equilibriums may exist for a given system. We also suggest how the work of Wilson et al. could be extended theoretically and empirically to further capture the effects of receiver psychology on selective pressures on multi-component and multi-modal signals. We also highlight the assumptions and predictions of the existing theory that would most benefit from and would be most amenable to empirical testing.     

Cardenolide content,emetic potency,and thin-layer chromatography profiles of monarch butterflies,Danaus plexippus,and their larval host-plant milkweed,Asclepias humistrata,in Florida

Summary This paper is the fourth in a series on cardenolide fingerprints of monarch butterflies (Danaus plexippus, Danainae) and their host-plant milkweeds (Asclepiadaceae) in the eastern United States. Cardenolide concentrations ofAsclepias humistrata plants from north central Florida ranged from 71 to 710 µg/0.1 g dry weight, with a mean of 417 µg/0.1 g. Monarchs reared individually on these plants contained cardenolide concentrations ranging from 243 to 575 µg/0.1 g dry weight, with a mean of 385 µg/0.1 g. Cardenolide uptake by butterflies was independent of plant concentration, suggesting that sequestration saturation occurs in monarchs fed cardenolide-rich host plants. Thinlayer chromatography resolved 19 cardenolides in the plants and 15 in the butterflies. In addition to humistratin,A. humistrata plants contained several relatively non-polar cardenolides of the calotropagenin series which are metabolized to more polar derivatives in the butterflies. These produced a butterfly cardenolide fingerprint clearly distinct from those previously established for monarchs reared on otherAsclepias species. In emetic assays with the blue jay,Cyanocitta cristata, the 50% emetic dose (ED₅₀) per jay was 57.1 µg, and the average number of ED₅₀ units per butterfly was 13.8, establishing that this important south eastern milkweed produces highly emetic, chemically defended monarchs. Our data provide further support for the use of cardenolide fingerprints of wild-caught monarchs to make ecological predictions concerning defence against natural enemies, seasonal movement and larval host-plant utilization by monarch butterflies during their annual cycle of migration, breeding and overwintering.     

Environmental problems of coal production in the United Kingdom with particular reference to the Yorkshire coalfield

This paper reviews the environmental problems associated with the coal-mining industry on the Yorkshire coalfield. The main environmental problems are identified as being caused by:

1. The need to dispose of large quantities of colliery waste or ‘spoil’, in an environmentally acceptable manner.
2. Derelict and despoiled land, including the problems caused by the re-working of old tips for their coal content.
3. Opencast coal mining.
4. Subsidence.
5. Transportation of coal and coal waste.

These problems are reviewed in the context of the role of coal in meeting the United Kingdom's energy needs and the framework of environmental control within which the coal-mining industry has to operate. Particular stress is laid on the fact that the Yorkshire coalfield, as with the majority of coalfields, is an old one with a legacy of inherited dereliction. Whilst modernisation of the mining industry is proceeding there have not been parallel developments put in hand to cope with the changing nature of the environmental problems resulting from the modernisation of the industry. Particular stress is placed on the need to dispose of the greatly increased quantities of spoil now being produced.     

Geese and dietary allelochemicals — food palatability and geophagy

Summary The palatability of plants was studied in young, immature and adult geese of three species,Anser anser, Anser indicus andBranta canadensis, with respect to secondary plant metabolites. In their first 1–4 weeks of life, hand-reared goslings feed on a wide variety of plants, more or less irrespective of their allelochemical contents. Older birds become more selective, but still consume plants which are normally considered to be unpalatable or even toxic for other animals. Choice experiments were performed with pure secondary metabolites which were offered on otherwise highly palatable food items, such as leaves ofTaraxacum officinale. These experiments revealed a similar trend, in that very young goslings discriminate their food much less than older goslings or adult geese. In general, food contaminated with essential oils was rejected, whereas alkaloids, glycosides, amines and sulfur compounds were tolerated to a remarkable degree. In consequence, especially young, but also adult geese must have a high capacity to tolerate and/or to detoxify dietary allelochemicals. Another detoxification mechanism became evident during the experiments: geese ingest soil and mud quite regularly. It could be shown experimentally that the respective soil had a high capacity to bind alkaloids. We assume that geophagy is a means (besides a presumed active detoxification in the liver) to adsorb and thus reduce the contents of dietary allelochemicals.     

Teil 3: PCDD/F im Blutfett ausgewählter Personen

This study was designed to assess the degree of exposure to PCDD/F in 56 selected persons living in the vicinity of a former copper smelter located in Marsberg, Germany. The copper smelter was in operation until 1945. In 1991, high levels of PCDD/F were found particularly in materials from the slag dumps (10 000 – 100 000 ng TE/kg). The levels of PCDD/F in blood fat were used as parameters for individual PCDD/F exposure. Since only limited reference data for PCDD/F in human blood were available, blood samples of a reference group were analyzed in the same way. The criteria were collected from a group matched for sex, age (±3 years) and body mass. The median NATO/CCMS-Toxicity equivalency values (NATO/CCMS-TE-values) of the Marsberg group (43,2 pg NATO/CCMS-TE/g blood fat) and the reference group (43,0 pg NATO/CCMS-TE/g blood fat) were similar, whereas the arithmetic mean of the Marsberg group (52,7 pg NATO/CCMS-TE/g blood fat) was higher than that of the reference group (44,4 pg NATO/CCMS-TE/g blood fat). The frequency distribution of the individual TE-values of the Marsberg group was slightly shifted toward higher values. The individuals of the Marsberg group had significantly higher levels of PentaCDF, HexaCDF and HeptaCDF on average than the individuals of the reference group. On the basis of TE-values, the majority of the individuals from Marsberg did not have an increased body burden of PCDD/F. However, some individuals could be identified with TE-values exceeding background levels ranging up to 231 pg NATO/CCMS-TE/g blood fat. Although it must be assumed that the unusual congener pattern found in many of the test subjects is a result of ingestion of contaminated slag material or soil, the findings of this study could not verify this fact.     

Trace-element speciation and partitioning in environmental geochemistry and health

Establishment of the chemical form and associations of trace elements is important in the scientific and medical fields related to environmental geochemistry and health. Fundamental understanding of trace-element behaviour, the realistic formulation of historical perspectives of trace-element contamination, an assessment of environmental transformation processes and a thorough appraisal of environment-related ill health and disease all depend on knowledge of the chemical speciation and partitioning of trace elements. These topics and the development of analytical speciation techniques and procedures are discussed with reference to trace-element studies in the Department of Forensic Medicine and Science, University of Glasgow, on lacustrine sediments and water, the atmosphere, soil and street dirt of an urban environment and human biological fluids.     

Automatic time-series quantification of bluff erosion using a single consumer grade camera as basis for erosion risk assessment and forecasts – a Boston Harbor Islands case study

Many communities along coastlines and riverbanks are threatened by water erosion and hence an accurate model to predict erosion events is needed in order to plan mitigation strategies. Such models need to rely on readily available meteorological data that may or may not be correlated with the occurrence of erosion events. Computing these correlations requires a quantified index that reports the magnitude of erosion events over time. This study introduces a method to create erosion indices using affordable consumer grade digital cameras. It is able to detect and quantify erosion using an image series obtained from just one such camera by segmenting each images instance into equally sized squares that can be preprocessed and analyzed separately. This approach isolates each image segment from noise and temporary disturbances that frequently occur throughout images taken with low cost cameras. In this fashion, noise may either be addressed locally or simply ignored if it is too extreme. After preprocessing, comparison of subsequent segments yields change matrices that form the basis for segment-specific erosion indices that are later combined into a composite value for the entire image instance. Whenever a segment instance is unavailable or unusable, the algorithm attempts to use neighboring instances whenever possible. When tested against human observation of erosion events during a 6 week period, the resulting index achieves a true positive rate of 67% while producing only a small number of false positives. Finally, the index is validated by significant correlation with various meteorological data streams.