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521.
Nathan L. Kirk Jason P. Andras C. Drew Harvell Scott R. Santos Mary Alice Coffroth 《Marine Biology》2009,156(8):1609-1623
Numerous marine invertebrates form endosymbiotic relationships with dinoflagellates in the genus Symbiodinium. However, few studies have examined the fine-scale population structure of these symbionts. Here, we describe the genetic
structure of Symbiodinium type “B1/B184” inhabiting the gorgonian Gorgonia
ventalina along the Florida Keys. Six polymorphic microsatellite loci were utilized to examine 16 populations along the Upper, Middle,
and Lower Keys spanning a range of ~200 km. Multiple statistical tests detected significant differentiation in 54–92% of the
120 possible pairwise comparisons between localities, suggesting low levels of gene flow in these dinoflagellates. In general,
populations clustered by geographic region and/or reefs in close proximity. Some of the sharpest population differentiation
was detected between Symbiodinium from deep and shallow sites on the same reef. In spite of the high degree of population structure, alleles and genotypes
were shared among localities, indicating some connectivity between Symbiodinium populations associated with G. ventalina.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
522.
Ecologists seek better understanding of why species interactions change across space and time in natural communities. In streams, species effects on resources and community structure may change as physical characteristics of the stream environment change along drainage networks. We examined spatial and seasonal effects of armored grazers using a small-scale exclusion experiment that was replicated in streams of different drainage areas. Effects of grazing varied with stream size and were related to variation in grazer abundance and phenology. We identified three distinct grazing regimes and a stream size (drainage area [DA]) threshold corresponding to a shift from one to two functional trophic levels. In streams with DA < 1 km2, armored grazers did not reduce biomass of algal biofilms. In slightly larger streams (2-3 km2 DA), the armored grazer guild was dominated by bivoltine Glossosoma. These caddisflies persisted and limited algal biofilms throughout the summer in one of these streams. In the largest tributaries (DA > 10 km2), the grazer guild was dominated by univoltine caddisflies, and grazing limited algal biofilms in early summer, but not late summer, after caddisflies pupated. Drainage area is a useful predictor of spatial transitions in food web interactions within and among watersheds. Quantifying the drainage area threshold at which interactions change in catchments with differing geology, vegetation, hydrology, climate, land use, or species pools should help build the understanding we need to forecast ecological responses to environmental change. 相似文献
523.
A meta-BACI approach for evaluating management intervention on chronic wasting disease in mule deer.
Mary M Conner Michael W Miller Michael R Ebinger Kenneth P Burnham 《Ecological applications》2007,17(1):140-153
Advances in acquiring and analyzing the spatial attributes of data have greatly enhanced the potential utility of wildlife disease surveillance data for addressing problems of ecological or economic importance. We present an approach for using wildlife disease surveillance data to identify areas for (or of) intervention, to spatially delineate paired treatment and control areas, and then to analyze these nonrandomly selected sites in a meta-analysis framework via before-after-control impact (BACI) estimates of effect size. We apply these methods to evaluate the effectiveness of attempts to reduce chronic wasting disease (CWD) prevalence through intensive localized culling of mule deer (Odocoileus hemionus) in north-central Colorado, USA. Areas where surveillance data revealed high prevalence or case clusters were targeted by state wildlife management agency personnel for focal scale (on average <17 km2) culling, primarily via agency sharpshooters. Each area of sustained culling that we could also identify as unique by cluster analysis was considered a potential treatment area. Treatment areas, along with spatially paired control areas that we constructed post hoc in a case-control design (collectively called "management evaluation sites"), were then delineated using home range estimators. Using meta-BACI analysis of CWD prevalence data for all management evaluation sites, the mean effect size (change of prevalence on treatment areas minus change in prevalence on their paired control areas) was 0.03 (SE = 0.03); mean effect size on treatment areas was not greater than on paired control areas. Excluding cull samples from prevalence estimates or allowing for an equal or greater two-year lag in system responses to management did not change this outcome. We concluded that management benefits were not evident, although whether this represented true ineffectiveness or was a result of lack of data or insufficient duration of treatment could not be discerned. Based on our observations, we offer recommendations for designing a management experiment with 80% power to detect a 0.10 drop in prevalence over a 6-12-year period. 相似文献
524.
Quantitative threat analysis for management of an imperiled species: Chinook salmon (Oncorhynchus tshawytscha). 总被引:1,自引:0,他引:1
Jonathan M Hoekstra Krista K Bartz Mary H Ruckelshaus Jennifer M Moslemi Tamara K Harms 《Ecological applications》2007,17(7):2061-2073
Chinook salmon (Oncorhynchus tshawytscha) have declined dramatically across the Pacific Northwest because of multiple human impacts colloquially characterized as the four "H's": habitat degradation, harvest, hydroelectric and other dams, and hatchery production. We use this conceptual framework to quantify the relative importance of major threats to the current status of 201 Chinook populations. Current status is characterized by two demographic indices: population density and trend. We employ path analytic models and information theoretic methods for multi-model inference. Our results indicate that dams most strongly affect variation in population density, while harvest and hatchery production most strongly affect variation in population trend. Comparable results arise when the sample size of the analysis is reduced to 22 Chinook populations within a smaller region typical of the scale at which salmon recovery planning is conducted. Results from these threat analyses suggest that recovery strategies targeting specific demographic indices, and those considering natural and human-mediated interdependencies of major threats, are most likely to succeed. 相似文献
525.
David WATSON Carrie MILLER Brian LESTER Kenneth LOWE George SOUTHWORTH Mary Anna BOGLE Liyuan LIANG Eric PIERCE 《Frontiers of Environmental Science & Engineering》2015,9(4):596
Development and demonstration of reliable measurement techniques that can detect and help quantify the nature and extent of elemental mercury (Hg(0)) in the subsurface are needed to reduce uncertainties in the decision-making process and increase the effectiveness of remedial actions. We conducted field tests at the Y-12 National Security Complex in Oak Ridge, Tennessee, USA, to determine if sampling and analysis of Hg(0) vapors in the shallow subsurface (<0.3 m depth) can be used to as an indicator of the location and extent of Hg(0) releases in the subsurface. We constructed a rigid polyvinyl chloride push probe assembly, which was driven into the ground. Soil gas samples were collected through a sealed inner tube of the assembly and were analyzed immediately in the field with a Lumex and/or Jerome Hg(0) analyzer. Time-series sampling showed that Hg vapor concentrations were fairly stable over time, suggesting that the vapor phase Hg(0) was not being depleted and that sampling results were not sensitive to the soil gas purge volume. Hg(0) vapor data collected at over 200 push probe locations at 3 different release sites correlated very well to areas of known Hg(0) contamination. Vertical profiling of Hg(0) vapor concentrations conducted at two locations provided information on the vertical distribution of Hg(0) contamination in the subsurface. We conclude from our studies that soil gas sampling and analysis can be conducted rapidly and inexpensively at large scales to help identify areas contaminated with Hg(0). 相似文献
526.
Various heavy metals released into the rivers and lakes affect aquatic fauna including Clarias batrachus. Elevated levels of chromium (Cr) in aquatic organisms, especially fish, represent an ecological and human concern. Amino acid levels were estimated in five tissues (gills, liver, kidneys, muscle, and brain) of C. batrachus after 28 days of exposure to Cr. This study showed that Cr elevated levels of free amino acids in organs of fish C. batrachus, indicating a compensatory mechanism to counter sublethal metallic stress. Data suggest that the measurement of amino acids may serve as a biomarker for Cr toxicity in fish. 相似文献
527.
IntroductionWe interviewed fishermen to identify jobs and/or tasks of concern in order to guide and inform current ergonomic interventions.MethodsWe administered surveys to two groups of southeastern US commercial crab fishermen: a cohort contacted by telephone and fishermen recruited at 11 local fish houses. Fishermen were asked to rate physical stress of selected work tasks and conditions on a scale from 0 (no problem) to 10 (major problem). Fishermen were also asked open-ended questions about other work-issues of concern.ResultsFishermen who completed the survey (n = 91) were predominantly male (90%), white (96%), and over 40 years of age (71%). The five most physically strenuous tasks and conditions as rated by fishermen were pulling pots by hand (mean 6.4), rough weather (6.1) or rough water (6.2), unloading without mechanical assistance (5.8), and long work days (5.3). Task stress ratings with the most variability measured by the inter-quartile range (IQR) included: pulling pots by hand (IQR = 5), unloading without mechanical assistance (IQR = 5), gear maintenance (IQR = 4.5), and moving boxes and baskets on board (IQR = 4.5). Other situations that fishermen identified as a problem included: economic, off-shore work, declining industry, mental stress, environment, regulation, conflict with other commercial or recreational fishermen, issues with crew, sun (glare or burn), and breaking up frozen bait.ConclusionsIn general, stressful tasks identified from previous research were rated similarly by the fishermen surveyed. Development and testing of ergonomic interventions is ongoing. 相似文献
528.
Amador JA Potts DA Savin MC Tomlinson P Görres JH Nicosia EL 《Journal of environmental quality》2006,35(4):1160-1169
Aeration improves the capacity of leachfields to decontaminate and reduce the nutrient load of wastewater. To gain a better understanding of the effects of aeration, we examined the faunal and microbial communities of septic system leachfield soil (0-4 and 4-13 cm) using replicated (n = 3) mesocosms that were actively aerated (AIR) or unaerated (LEACH). Protozoa were 40 to 140 times more abundant in AIR than in LEACH soil. No nematodes were found in LEACH soil, whereas AIR soil contained 5 to 14 x 10(3) nematodes (all bacteriovores) kg(-1). Active microbial biomass was four to five times higher in AIR than LEACH soil. Proteobacteria and actinomycetes/sulfate-reducing bacteria constituted a higher proportion of the community in AIR soil, whereas anaerobic Gram-negative bacteria/firmicutes were more prominent in LEACH soil. Ratios of prokaryotic to eukaryotic phospholipid fatty acids (PLFAs) were higher in LEACH soil, as were membrane stress index values, whereas the starvation index was higher in AIR soil. Community-level physiological profiles showed that 29 and 30 different substrates were used for growth by LEACH and AIR soil microorganisms, respectively. The AIR soil had more microorganisms capable of growing on 10 substrates, whereas growth on two substrates was higher in LEACH soil. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis of 16S rRNA gene fragments revealed greater diversity of dominant phylotypes in AIR than LEACH soil, with communities separated by treatment. Aerated leachfield soil had a larger and more diverse faunal and microbial community than unaerated soil, possibly due to differences in the type and availability of electron acceptors. 相似文献
529.
Andrzej Bytnerowicz Dan Cayan Philip Riggan Susan Schilling Philip Dawson Mary Tyree Lynn Wolden Robert Tissell Haiganoush Preisler 《Atmospheric environment (Oxford, England : 1994)》2010,44(5):678-687
Combustion emissions and strong Santa Ana winds had pronounced effects on patterns and levels of ambient ozone (O3) in southern California during the extensive wildland fires of October 2007. These changes are described in detail for a rural receptor site, the Santa Margarita Ecological Reserve, located among large fires in San Diego and Orange counties. In addition, O3 changes are also described for several other air quality monitoring sites in the general area of the fires. During the first phase of the fires, strong, dry and hot northeasterly Santa Ana winds brought into the area clean continental air masses, which resulted in minimal diurnal O3 fluctuations and a 72-h average concentration of 36.8 ppb. During the second phase of the fires, without Santa Ana winds present and air filled with smoke, daytime O3 concentrations steadily increased and reached 95.2 ppb while the lowest nighttime levels returned to ~0 ppb. During that period the 8-h daytime average O3 concentration reached 78.3 ppb, which exceeded the federal standard of 75 ppb. After six days of fires, O3 diurnal concentrations returned to pre-fire patterns and levels. 相似文献
530.
Sholtz RI McLaughlin KR Cirillo PM Petreas M Park JS Wolff MS Factor-Litvak P Eskenazi B Krigbaum N Cohn BA 《Environment international》2011,37(4):709-714
Conserving irreplaceable, archived serum samples may sometimes conflict with the objective of minimizing measurement error due to laboratory effects. We sought to determine whether we could successfully combine assay results for DDT-related compounds and polychlorinated biphenyls (PCBs) in serum from the same birth cohort obtained from different laboratories over time. Using the Child Health and Development Studies (CHDS) serum archive, we compared variability for assays of a quality control pool to variability for assays of subject serum. The quality control pool was created from native archived serum samples that were pooled, then aliquoted, blinded and inserted pair-wise into assay batches along with the subject serum for 5 studies using CHDS samples conducted over a 13year period by three different laboratories. We found that the variability between laboratory and over time within laboratory was small relative to inter-individual variability for p,p'-DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane), p,p'-DDE (1,1'-dichloro-2,2'-bis(p-chlorophenyl)ethylene) and o,p'-DDT (1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl)-ethane). Results were also consistent for most PCB congeners which were detectable in 85% or more of samples. Our results suggest that it is possible to combine assays for DDT and PCB congeners measured at positive levels as they are accumulated for cohort subjects without risking meaningful misclassification due to variation stemming from laboratory or time period. This has significant implications for future study costs, conservation of irreplaceable archived samples and for leveraging past investments for future research. For PCB congeners with very low levels, findings caution against pooling of assays without further exploration. 相似文献