The removal of microorganisms and organic micropollutants from wastewater during infiltration to aquifers after irrigation of farmland in the Tula Valley, Mexico

The Tula Valley receives untreated wastewater from Mexico City for agricultural irrigation, half of which infiltrates to aquifers from where drinking water is extracted. Samples of wastewater and infiltrated water from three areas of the valley were analyzed for microorganisms, organic micropollutants, and some basic parameters. Concentrations of microorganisms in the infiltrated water were generally very low but the incidence of fecal coliforms (present in 68% of samples), somatic bacteriophages (36%), Giardia spp. (14%), and helminth eggs (8%) suggested a health risk. Organic micropollutants, often present at high concentrations in the wastewater, were generally absent from the infiltrated water except carbamazepine which was in 55% of samples (up to 193 ng/L). There was no correlation between carbamazepine concentrations and the presence of microorganisms but highest concentrations of carbamazepine and boron coincided. A treatment such as nanofiltration would be necessary for the infiltrated water to be a safe potable supply.     

Integrating climate change adaptation into Dutch local policies and the role of contextual factors

Moving towards a more sustainable adaptation process requires closer integration of policies related to the environment. An important actor in this is the local government. This paper examines to what extend adaptation is currently being integrated into Dutch local policies, and what the role is of a municipality's size, risk and experience in the encountered manifestations of adaptation. First, it was determined that adaptations taking place only anticipate currently perceivable weather extremes – mostly increasing precipitation. Second, it was determined that the realisation of further adaptations is hindered by a strongly sectoral divided reality. Adaptation is now heavily dominated by the water department, while spatial planning and the environment are only limitedly involved. Finally, it was observed that the contextual factor size proved to most important for horizontal policy integration, whereas the contextual factor extreme-weather experience was the most definite for the realisation of adaptations. We conclude that a more sustainable adaptation should first tackle the sectoral divides which requires administrative efforts, for example, professional training. These would preferably be initiated from a vertical direction.     

A synomone imparting distinct sex pheromone communication channels for Choristoneura rosaceana (Harris) and Pandemis limitata (Robinson) (Lepidoptera: Tortricidae)

Summary. Male obliquebanded leafrollers, Choristoneura rosaceana (Harris), were induced to respond to a pheromone source tainted with a behavioural antagonist, Z9-tetradecenyl acetate, when a source releasing the antagonist was placed 10 cm upwind of the tainted source in a wind tunnel. However, placement of the antagonist upwind of an attractive pheromone source did not interrupt pheromone-mediated responses. Placement of a source releasing Z9-tetradecenyl acetate, a minor pheromone component of the sympatric species, the threelined leafroller, Pandemis limitata (Robinson), upwind of a calling P. limitata female, reduced conspecific male pheromone-mediated response but resulted in upwind flight by male C. rosaceana and contact with heterospecific females. Male P. limitata locked on and flew upwind to but did not contact heterospecific females when a source releasing Z9-tetradecenyl acetate was positioned upwind of a calling C. rosaceana female. In the field, adaptation or habituation to Z9-tetradecenyl acetate caused by atmospheric treatment with this compound apparently resulted in reciprocal heterospecific pheromone responses. More C. rosaceana males were captured in traps baited with their pheromone and the behavioural antagonist in small field plots treated atmospherically with Z9-tetradecenyl acetate than in nontreated control plots. Fewer male P. limitata were captured in traps baited with their own pheromone, or with C. rosaceana pheromone tainted with Z9-tetradecenyl acetate in plots treated atmospherically with Z9-tetradecenyl acetate than in nontreated control plots. We argue that Z9-tetradecenyl acetate is an important synomone which assists in partitioning the sexual chemical communication channels of C. rosaceana and P. limitata. Received 9 February 1999; accepted 22 March 1999.     

Identification of various laccases induced by anthracene and contribution to its degradation in a Mediterranean coastal pine litter

Mediterranean coastal ecosystems are known to be highly subject to natural and anthropic environmental stress. In this study, we examine the effects of anthracene as a common pollutant on the total microbial communities from a Pinus halepensis litter of a typical Mediterranean coastal site (Les Calanques, Marseille). The main objective was to identify the microbial factors leading the resilience of this ecosystem. Two questions were addressed: (i) how lignin-degrading enzymes (Laccase, Lignin-peroxidase and Mn-peroxidase) are affected by the presence of this molecule, (ii) whether the indigenous consortia are involved in its degradation in mesocosms under favorable incubation conditions (25 °C, 60% WHC) and after different time intervals (1 and 3 month(s)). We found a strong increase in laccase production in the presence of anthracene after 3 months, together with anthracene degradation (28% ± 5). Moreover 9,10-anthraquinone is detected as the product of anthracene oxidation after 3 months. However neither lignin-peroxidase activity nor Mn-peroxidase activity is detected. Laccase proteins directly extracted from litter were sequenced via Nano-LC-MS/MS and reveal twelve different peptide sequences induced by the presence of anthracene in the mesocoms. Our study confirms the major detoxification role of this enzymatic system and highlights the high degradation potential of fungal species inhabiting P. halepensis litter, a factor in the resilience of Mediterranean ecosystems.     

Glutathione antioxidant status of frozen meat samples

Lipid oxidation is a process, which results in rancidity and deterioration of fats posing a major problem in food industry. Antioxidants are of interest, which presumably protects food from oxidative deterioration during storage. The glutathione antioxidant system of different meat samples were noticed for six months under refrigerated storage. Activity of glutathione reductase (GR) and level of glutathione (GSH) decreased during six months storage in all the four meat samples. The glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activity increased gradually during storage. It seems possible that glutathione antioxidant system protected the meat samples against quality loss during its storage.     

A comparison of five high-resolution spatially-explicit,fossil-fuel,carbon dioxide emission inventories for the United States

The quantification of fossil-fuel-related emissions of carbon dioxide to the atmosphere is necessary in order to accurately represent carbon cycle fluxes and to understand and project the details of the global carbon cycle. In addition, the monitoring, reporting, and verification (MRV) of carbon dioxide emissions is necessary for the success of international agreements to reduce emissions. However, existing fossil-fuel carbon dioxide (FFCO₂) emissions inventories vary in terms of the data and methods used to estimate and distribute FFCO₂. This paper compares how the approaches used to create spatially explicit FFCO₂ emissions inventories affect the spatial distribution of emissions estimates and the magnitude of emissions estimates in specific locales. Five spatially explicit FFCO₂ emission inventories were compared: Carbon Dioxide Information and Analysis Center (CDIAC), Emission Database for Global Atmospheric Research (EDGAR), Fossil Fuel Data Assimilation System (FFDAS), Open-source Data Inventory for Anthropogenic CO₂ (ODIAC), and Vulcan. The effects of using specific data and approaches in the creation of spatially explicit FFCO₂ emissions inventories, and the effect of resolution on data representation are analyzed using graphical, numerical, and cartographic approaches. We examined the effect of using top-down versus bottom-up approaches, nightlights versus population proxies, and the inclusion of large point sources. The results indicate that the approach used to distribute emissions in space creates distinct patterns in the distribution of emissions estimates and hence in the estimates of emissions in specific locations. The different datasets serve different purposes but collectively show the key role of large point sources and urban centers and the strong relationship between scale and uncertainty.