Regional Environmental Change - By 2014 approximately 2.2 million km2 (~43%) of Brazil’s Legal Amazonia region had been incorporated into an extensive network of 718 protected areas, which... 相似文献
In the current study it was aimed to investigate the toxicity of low doses of imidacloprid (IMI) on the reproductive organ systems of adult male rats. The treatment groups received 0.5 (IMI-0.5), 2 (IMI-2) or 8 mg IMI/kg body weight by oral gavage (IMI-8) for three months. The deterioration in sperm motility in IMI-8 group and epidydimal sperm concentration in IMI-2 and IMI-8 groups and abnormality in sperm morphology in IMI-8 were significant. The levels of testosterone (T) and GSH decreased significantly in group IMI-8 compared to the control group. Upon treatment with IMI, apoptotic index increased significantly only in germ cells of the seminiferous tubules of IMI-8 group when compared to control. Fragmentation was striking in the seminal DNA from the IMI-8 group, but it was much less obvious in the IMI-2 one. IMI exposure resulted in elevation of all fatty acids analyzed, but the increases were significant only in stearic, oleic, linoleic and arachidonic acids. The ratios of 20:4/20:3 and 20:4/18:2 were decreased and 16:1n-9/16:0 ratio was increased. In conclusion, the present animal experiments revealed that the treatment with IMI at NOAEL dose-levels caused deterioration in sperm parameters, decreased T level, increased apoptosis of germ cells, seminal DNA fragmentation, the depletion of antioxidants and change in disturbance of fatty acid composition. All these changes indicate the suppression of testicular function. 相似文献
The classic Microtox® solid phase assay (MSPA) based on the inhibition of light production of the marine bacteria recently renamed Aliivibrio fischeri suffers from various bias and interferences, mainly due to physico-chemical characteristics of the tested solid phase. To precisely assess ecotoxicity of sediments, we have developed an alternative method, named Microtox® leachate phase assay (MLPA), in order to measure the action of dissolved pollutants in the aqueous phase. Two hypotheses were formulated to explain the observed difference between MSPA and MLPA results: a real ecotoxicity of the solid phase or the fixation of bacteria to fine particles and/or organic matter. To estimate the latter, flow cytometry analyses were performed with two fluorochromes (known for their ability to stain bacterial DNA), allowing correction of MSPA measurements and generation of new (corrected) IC50. Comparison of results of MLPA with the new IC50 MSPA allows differentiating real ecotoxic and fixation effect in classic MSPA especially for samples with high amount of fines and/or organic matter. 相似文献
This study examined the tissues distribution of selected serotonin reuptake inhibitors (SSRIs) in brook trout exposed for 3 months to continuous flow-through primary-treated effluent before and after ozone treatment. A reliable analytical method was developed for the quantification of trace amounts of antidepressants in small tissue homogenate extracts. Levels of six antidepressants and four of their N-desmethyl metabolites were determined using liquid chromatography-tandem mass spectrometry. Significant amounts of the SSRIs were found in fish tissue-in decreasing order: liver>brain>muscle. Sertraline and its metabolite desmethylsertraline were the predominant substances observed in most tissues (0.04-10.3 ng g(-1)). However, less SSRIs (0.08-1.17 ng g(-1)) were bioaccumulated in the ozonated effluent. The early molecular effects of these SSRIs on the Na/K-dependent ATPase pump activity in brain synaptosomes where also investigated in vitro and in fish exposed to the municipal effluents. With respect to their potential biological effects, in vitro exposure to selected SSRIs induced a reduction of the brain Na/K-ATPase activity in synaptosomes in a dose-dependent manner. Results showed that Na/K-ATPase activity was readily inhibited by exposure to municipal effluent before and, to a lesser extent, after ozone treatment. Moreover, the Na/K-ATPase activity was significantly and negatively correlated with brain tissue concentrations of fluoxetine (r=-0.57; p<0.03), desmethylsertraline (r=-0.84; p<0.001), and sertraline (r=-0.82; p<0.001). The present study reveals that SSRIs are readily available in fish, biologically active and corroborates previous findings on the serotonergic properties of municipal effluents to aquatic organisms. 相似文献
Plasticized starch (PLS) is a renewable, degradable, and inexpensive polymer, but it suffers from poor mechanical properties.
The mechanical properties can be improved by blending PLS with polyolefins, nonetheless, at high PLS content, the mechanical
properties remain poor. Here we show that addition of clay can greatly improve the mechanical properties of PLS/polypropylene
blends at high starch content. Unmodified and organically modified montmorillonite clays, MMT and Cloisite 30B respectively,
were added to blends of glycerol-plasticized starch and polypropylene, compatibilized using maleated polypropylene. TEM indicates
that MMT is well dispersed in the PLS phase of the blends, while Cloisite 30B is located both within the PLS phase as well
as at the interface between PLS and PP. At high PLS content, the addition of clay increased the tensile strength and tensile
modulus by an order of magnitude, while reducing the ultimate elongation only slightly. Such improvements are attributable
to both the addition of clay as a reinforcing component, as well as to the change in the two phase morphology due to addition
of clay. 相似文献
Rodent outbreaks affect many farmland areas worldwide and the negative environmental impacts of control campaigns cause intense social tensions. In such conservation conflicts, understanding stakeholders’ viewpoints is critical to promote ecologically sustainable management. We used Q-methodology, a framework standing between qualitative and quantitative social research, to investigate human subjectivity and understand conflicts caused by rodent outbreaks in Spain. We interviewed farmers, conservationists, hunters, and governmental agencies, and identified five main discourses about the origins and consequences of the conflictive situation. Finding sustainable management is impaired by opposing views about causes and consequences of vole outbreaks and their management, which are at the root of the conflict. Social tensions will likely remain until the underlying conflicts between people holding different views are also managed. Decision-making should therefore focus on mitigating underlying conflicts. Using trained independent mediators would help the effective resolution of conservation conflicts caused by rodent outbreaks and their management.
AbstractThe main objective of this study is the degradation of a synthetic solution of atrazine by a modified vermiculite catalyzed ozonation, in a rotating packed bed (RPB) reactor. A 0.5?L RPB reactor was used to perform the experiments, using a Central Composite Design (CCD) response surface to construct the quadratic model based on the factors: pH, catalyst concentration and reactor rotation frequency. The response variable was the removal of the organic load measured in terms of Chemical Oxygen Demand (COD). After the complete quadratic model was constructed through the response surface, the COD degradation process had an optimal removal of 41% under the following conditions: pH 8.0, rotation of 1150?rpm and catalyst concentration 0.66?g L?1. 相似文献
Sediment cores from oxygen depleted zones of lake Léman (lake of Geneva) and from anoxic surface sediment from Voua de la Motte (France) were analyzed for their sterol compositions. 4-methylstanols were found to be major sterols in Motte lake sediment, which is rich in organic matter. In lake Léman, the relative abundances of the same 4-methylstanols increase with the depth in the core, but they remain always minor compounds. An extensive search for a possible external source for these compounds included the analysis of water, of the plankton and of the surrounding soil material. All these results, along with those of an in situ incubation experiment, give strong evidence in favor of their in situ bacterial biosynthesis. 相似文献