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91.
Pinniped Penises in Trade: A Molecular-Genetic Investigation 总被引:1,自引:0,他引:1
S. Malik P. J. Wilson R. J. Smith D. M. Lavigne & B. N. White 《Conservation biology》1997,11(6):1365-1374
92.
The encrusting spongeHalisarca laxus forms a seemingly obligate association with the stalked solitary ascidianPyura spinifera. In 1991 we examined spatial variation and short-term temporal variation in this association at three neighbouring sites in southeastern Australia. This sponge dominated the surface of almost all the 500 individual ascidians examined, with mean cover usually exceeding 90%. This pattern was consistent among sites and throughout the year of the study. The domination of a small isolated patch of habitable substratum by a sponge is most unusual, given that they are regarded as relatively poor recruiters. To understand how this association might be maintained, we determined the underlying genotypic diversity of the sponge population using starch-gel electrophoresis.P. spinifera is a clump-forming ascidian and usually occurs in clumps of up to 22 individuals. Electrophoretic surveys, based on six variable allozyme loci, revealed that at a total of five plots within three neighbouring New South Wales populations, single sponge genotypes may cover entire ascidian clumps; although a clump sometimes played host to more than one sponge clone. Allele frequencies (averaged across four loci that appear to conform to Mendelian inheritance) showed little variation among populations (standardised genetic variance,F
ST=0.013). Nevertheless, sponge populations were genotypically diverse, with samples from 63 of 172 individual clumps displaying unique clonal genotypes. Moreover, multi-locus genotypic diversity within all sites approached the level expected for sexual reproduction with random mating. Taken together, these data imply thatH. laxus produces sexually-derived larvae that are at least moderately widelly dispersed. Given the relatively small size of the patches that this sponge inhabits, we also conclude that these larvae are good colonists and good spatial competitors on their ascidian hosts. 相似文献
93.
Y. Verlinsky N. Ginsberg M. Chmura M. Freidine M. White C. Strom A. Kuliev 《黑龙江环境通报》1995,15(9):831-834
In a routine application of commercially available centromeric DNA probes for the prenatal screening of common trisomies involving the autosomes 13, 18, and 21, and sex chromosomes, four cases of discrepancy between fluorescence in situ hybridization (FISH) results and follow-up cytogenetic analysis were observed from a total of 516 cases of amniocentesis. In three of these cases, the results were false negative, and in one false positive. In this case, amniocentesis was performed because of a positive triple test in a 34-year-old woman with previous infertility treatment. The alpha satellite DNA probe for chromosomes 13/21 revealed five signals in 50 per cent of uncultured amniocytes, while standard cytogenetic analysis showed a normal karyotype. FISH analysis on metaphase chromosomes demonstrated the location of the additional signal in the centromeric region of chromosome 22. This additional signal was also present in the centromeric region of chromosome 22 of the mother, providing evidence for a possible inherited polymorphism in chromosome 22 responsible for unspecific hybridization with the alpha satellite probe for chromosomes 13/21 in this case. The observed polymorphism in centromeric regions may contribute to unreliability of the use of the 13/21 alpha satellite probe for prenatal screening by FISH. 相似文献
94.
The lengths-at-age of individuals of the nervous shark Carcharhinus cautus in Shark Bay, Western Australia, have been determined and used to explore the types of situation when it might be advisable to shift from employing a von Bertalanffy equation to a more complex equation for describing the growth of this species and of elasmobranchs in general. The reproductive biology of C. cautus was also examined in order to construct curves for describing growth throughout life from conception as well as from parturition. The presence, in November and early December, of fresh bite marks on the sides of mature females and of a very high proportion of spent individuals among mature males indicate that C. cautus copulates in late October/early November. Ovulation and conception occur in late November/early December and parturition takes place approximately 11 months later. Since mature non-pregnant females contain vitellogenic ova for 12-13 months, i.e. from November or December to the following December, and mature pregnant females contain embryos for 11 months, i.e. from December to October, C. cautus has a biennial reproductive cycle. By parturition, the females and males of C. cautus had reached ~28% and 32% of their lengths at their maximum observed ages, respectively. The maximum recorded total lengths and ages of females and males of C. cautus were 133 cm and 16 years and 111 cm and 12 years, respectively. Females and males reached maturity at ~101 and ~91 cm, respectively, and at least 50% of females and males had become mature by the end of their sixth and fourth years of life after parturition, respectively. The three-parameter, von Bertalanffy growth curves provided reasonably good fits to the lengths-at-age of females and males of C. cautus during just postnatal life and throughout the whole of pre- and postnatal life. While the four-parameter, Schnute growth curve significantly improved the fit to these data for both females and males from conception and for females from parturition, it was recognised that the likelihood ratio test is very sensitive when, as in these cases, there are a large number of data points. A number of interrelated factors were thus taken into account when discussing circumstances when it might be appropriate to switch from using a von Bertalanffy growth curve to the more complex Schnute growth curve. 相似文献
95.
F. White 《Marine Biology》1969,4(4):333-339
The distribution of Trypetesa lampas (Hanoock) and its rates of infection of different species of gastropod shells in the waters of Anglesey were studied. T. lampas was found in depths from 3 to at least 25 fathoms (5 to 46 m). It infected dead shells, mainly of Buccinum undatum L., Colus gracilis (da Costa), Neptunea antiqua (L.) and Natica alderi (Forbes); it was absent from shells occupied by hermit crabs in the intertidal zone. Significantly more B. undatum shells were infected than shells of N. antiqua and Natica alderi, and more of both C. gracilis and N. antiqua than of Natica alderi. Larger shells of B. undatum were more likely to be infected than were the smaller ones. The number of females per shell varied with the shell length raised to the power 2.39 instead of as expected with the square of the length. 相似文献
96.
Jason E Bruggeman Robert A Garrott Daniel D Bjornlie P J White Fred G R Watson John Borkowski 《Ecological applications》2006,16(4):1539-1554
The influence of winter recreation on wildlife in Yellowstone National Park (YNP), Wyoming and Montana, USA, is a controversial issue. In particular, the effects of road grooming, done to facilitate snowmobile and snowcoach travel, on bison (Bison bison) ecology are under debate. We collected data during winters, from 1997 to 2005, on bison road use, off-road travel, and activity budgets to quantify temporal trends in the amount of bison road and off-road travel and to identify the ecological factors affecting bison movements and use of the groomed road system in the Madison-Gibbon-Firehole (MGF) area of YNP. Using model comparison techniques, we found bison travel patterns to be influenced by multiple, interacting effects. Road travel was negatively correlated with road grooming, and we found no evidence that bison preferentially used groomed roads during winter. Snow water equivalent, bison density, and the springtime melt period were positively correlated with both bison road and off-road travel. From behavioral scans on 68,791 bison, we found that travel is only a small percentage (11%) of all bison activity, with foraging comprising 67% of observations. Also, only 7% of traveling bison and 30% of foraging bison were displacing snow, and we suggest foraging, rather than traveling, is likely the major energetic cost to bison in winter. Bison utilize their own trail network, connecting foraging areas using stream corridors, geothermal pathways, and self-groomed travel routes. Our results indicate that temporal patterns in bison road travel are a manifestation of general travel behavior and that groomed roads in the MGF do not appear to be a major factor influencing bison ecology and spatial redistribution. We suggest that the changes in bison spatial dynamics during the past three decades have likely been the result of the natural phenomenon of density-dependent range expansion, rather than having been caused by the anthropogenic influence of road grooming. 相似文献
97.
Differential bioavailability of field-weathered p,p'-DDE to plants of the Cucurbita and Cucumis genera 总被引:2,自引:0,他引:2
White JC 《Chemosphere》2002,49(2):143-152
Field experiments were conducted to assess the bioavailability of weathered p,p'-DDE in soil to plants in the Cucurbita (squash, pumpkin) and Cucumis (cucumber, melon) genera. As expected, significant variability exists in the uptake of p,p'-DDE between plants of different genera. Root:soil concentration factors, defined as the ratio of p,p'-DDE (ng/g, dry weight) in the roots to that in the soil, approach 1.8 for cucumbers/melons and 16 for squash/pumpkin. However, significant differences were also observed among varieties of squash and pumpkin, with greater than an order of magnitude variation in the root:soil concentration factors and up to two orders of magnitude difference in the absolute amount of contaminant present within the plant. Although root systems routinely contain the highest concentration of p,p'-DDE (ng/g), this compartment comprises less than 2% of the total plant biomass. In all varieties but one, more than 86% of the extracted pollutant was in the shoot system. For two of varieties of Cucurbita pepo, concentrations of p,p'-DDE in the stems reached 1.1-2.2 mg/g and estimations of percent contaminant extraction from the soil ranged from 0.40% to 2.4%. These values approach those observed in the phytoremediation of heavy metals by "hyperaccumulating" species and indicate the potential for a plant-based remediation approach to soils contaminated with persistent organic pollutants. 相似文献
98.
Chun S Lee J Geyer R White DC Raman DR 《Journal of environmental science and health. Part. B》2005,40(5):741-751
A laboratory incubation study was conducted to investigate the effect of agricultural antibiotics (sulfamethazine, tylosin, and chlortetracycline) on the persistence and transformation of 17beta-estradiol in Sequatchie loam. We measured concentrations of 17beta-estradiol and its primary metabolite (estrone) in soils spiked with antibiotics and 17beta-estradiol. Dehydrogenase activity (DHA) was also measured as an indicator of the total microbial activity of the soils. The presence of antibiotics significantly decreased transformation of 17beta-estradiol to estrone. There was a positive correlation between the DHA and the concentrations of estrone in soil spiked with 17beta-estradiol only, implying that the reaction is mainly catalyzed by dehydrogenases. However, the positive correlation was weakened in soil spiked with 17beta-estradiol and antibiotics together. We recommend that any study evaluating the fate and transport of estrogenic hormones in soil should include the effect of agricultural antibiotics because antibiotics and estrogenic hormones are commonly excreted together in environmental samples. 相似文献
99.
Although recent work has shown that environmentally relevant concentrations of freshly neutralized aluminium (AI) are bioavailable and toxic to freshwater invertebrates, the genotoxicity of Al has not been examined. Here we show that freshly neutralized Al affects structure and function of the salivary gland polytene chromosomes of the ubiquitous chironomid larva Chironomus riparius over three generations. Exposure to 500 microg l-1 added Al for 24-25 days resulted in a significantly higher frequency of numerous somatic aberrations, while no structural aberrations were found in F1 controls and few in the second and third generation. Aberrations also included deletions of sections of chromosome G of C. riparius larvae as well as deletions of one or more Balbiani rings. Changes in functional activity included decreased activity of the Balbiani rings (BR), and an increase in the number of decondensed centromeres. The activity of the nucleolar organizer (NOR) significantly decreased in F1 chironomids exposed to Al, while in the F2 and F3 generations the NOR showed normal (high) activity. First generation chironomids were generally more susceptible to Al although no clear evidence of tolerance was apparent over three generations. The possible use of alterations in chironomid polytene chromosomes as biomarkers of trace metal pollution is discussed. 相似文献
100.
Jason A. Roney Bruce R. White 《Atmospheric environment (Oxford, England : 1994)》2006,40(40):7668-7685
Emissions from fugitive dust due to erosion of “natural” wind-blown surfaces are an increasingly important part of PM10 (particulate matter with sizes of 10 μm aerodynamic diameter) emission inventories. These inventories are particularly important to State Implementation Plans (SIP), the plan required for each state to file with the Federal government indicating how they will comply with the Federal Clean Air Act (FCAA). However, techniques for determining the fugitive dust contribution to over all PM10 emissions are still in their developmental stages. In the past, the methods have included field monitoring stations, specialized field studies and field wind-tunnel studies. The measurements made in this paper allow for systematic determination of PM10 emission rates through the use of an environmental boundary layer wind tunnel in the laboratory. Near surface steady-state concentration profiles and velocity profiles are obtained in order to use a control volume approach to estimate emission rates. This methodology is applied to soils retrieved from the nation's single largest PM10 source, Owens (dry) Lake in California, to estimate emission rates during active storm periods. The estimated emission rates are comparable to those obtained from field studies and lend to the validity of this method for determining fugitive dust emission rates. 相似文献