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11.
Eiji Haramoto Masaaki Kitajima Naohiro Kishida Hiroyuki Katayama Mari Asami Michihiro Akiba 《Food and environmental virology》2012,4(3):93-101
A nationwide survey of viruses, protozoa, and indicator microorganisms in drinking water sources of Japan was conducted. Among 64 surface water samples collected from 16 drinking water treatment plants, 51 (80?%) samples were positive for at least one of the 11 pathogen types tested, including noroviruses of genogroups I (positive rate, 13?%) and II (2?%), human sapoviruses (5?%), human adenoviruses of serotypes 40 and 41 (39?%), Cryptosporidium oocysts (41?%), and Giardia cysts (36?%). Total coliforms, Escherichia coli, and F-specific coliphages were detected in 63 (98?%), 33 (52?%), and 17 (27?%) samples, respectively, and E. coli was judged to be the most suitable indicator of pathogen contamination of drinking water sources. Genogroup-specific real-time PCR for F-specific coliphages revealed the presence of F-specific RNA coliphages of animal genogroup I and human genogroups II and III in 13 (41?%), 12 (39?%), and 1 (3?%), respectively, of 31 plaques isolated. 相似文献
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Michihiro Kitagawa Kentaro Sugiura Hiroko Omi Yoshiaki Akiyama Kiyoko Kanayama Masaru Shinya Tadao Tanaka Hirobumi Yura Haruhiko Sago 《黑龙江环境通报》2002,22(1):17-21
To isolate fetal cells from maternal blood, we developed a new method based on galactose-bearing conjugation. Nucleated red blood cells (NRBCs), which highly express galactose on their surface, were selectively attached to a substrate coated with a galactose-containing polymer via soybean agglutinin (SBA), a galactose-specific lectin. Cord blood samples were used to evaluate enrichment efficacy of NRBCs by this method. Blood samples were obtained from 131 pregnant women between 6 and 27 gestational weeks. After preliminary condensation of fetal cells by Ficoll gradient centrifugation, NRBCs were enriched using galactose-positive selection by adjusting SBA concentration. We isolated one to severalhundred NRBCs (mean±SD, 7.8±8.5) in 2.3 ml of peripheral blood samples from 96% of pregnantwomen. The isolated NRBCs were analyzed by a Y-chromosome FISH probe in eight cases carrying male fetuses. Y-signals were detected in all eight cases and more than half of the NRBCs wereoffetal origin. The study demonstrates that our new method using galactose-specific lectin provides effective enrichment of fetal NRBCs allowing non-invasive prenatal diagnosis. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
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Teruhiko Takahara Yukihiro Kohmatsu Atsushi Maruyama Ryohei Yamaoka 《Behavioral ecology and sociobiology》2008,63(2):235-240
When predator chemical cues are present, low activity of prey is a commonly seen defensive behavior. However, few studies
have explored the functional implications of the defensive behaviors and, thus, elucidated the possible linkages between behavioral
responses and its consequences. In this study, we experimentally investigated how behavioral responses of Hyla japonica tadpoles to predator chemical cues affect vulnerability to a dragonfly nymph Anax parthenope julius. The frequency of tadpoles attacked by dragonfly nymphs was lower with chemical cues of predator was present than without
chemical cues, and most of attacks occurred when tadpoles were mobile. When tadpoles were exposed to chemical cues, on the
other hand, their swimming speed was quicker and swimming distance was longer, respectively, and the rates of being approached
of the swimming tadpoles by dragonfly nymph was lower than those not exposed to chemical cues. We found that the tadpoles
are induced by predator chemical cues not only to generally lower activity but also to swim in bursts as additional behavior
and that the suite of their behavioral responses reduce the vulnerability against dragonfly nymph. Tadpoles can receive information
about the predation risks by chemical cues and adjust their defensive behavior accordingly. 相似文献
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Determination of nitrous oxide, methane, and ammonia emissions from a swine waste composting process 总被引:7,自引:0,他引:7
Takashi Osada Kazutaka Kuroda Michihiro Yonaga 《Journal of Material Cycles and Waste Management》2000,2(1):51-56
The amounts of harmful gas emissions from the process of composting swine waste were determined using an experimental composting
apparatus. Forced aeration (19.2–96.1 l/m3/min) was carried out continuously, and exhaust gases were collected and analyzed periodically. With weekly turning and the
addition of a bulking agent in order to decrease the moisture content and increase air permeability, the temperature of most
of the contents rose to 70°C and composting was complete within 3–5 weeks. NH3, CH4, and N2O emissions were high in the early stage of composting. About 10%–25% of the nitrogen in the raw material was lost as NH3 gas during composting. The emission rate of NH3 mainly depended on the aeration rate, so that as the aeration rate rose, the level of NH3 emissions increased. The CH4 and N2O emissions could be kept lower with adequate treatment at more than 40 l/m3/min aeration. N2O may be mainly the result of the denitrification of NO
x
-N in the additional matured compost used as a composting accelerator.
Received: September 11, 1998 / Accepted: November 8, 1999 相似文献
16.
Michihiro Mochida Kimitaka Kawamura Pingqing Fu Toshihiko Takemura 《Atmospheric environment (Oxford, England : 1994)》2010,44(29):3511-3518
Levoglucosan is considered as a useful molecular tracer of biomass-burning aerosols in the atmosphere. To characterize the seasonal variation of its concentrations over the Pacific Ocean and to assess its usefulness as a tracer after long-range transport, we investigated long-term variations of levoglucosan over Chichi-jima in the western North Pacific, from 2001 to 2004. Organic carbon (OC), elemental carbon (EC) and d-glucose were analyzed for comparison. The seasonal variation of levoglucosan concentrations showed a maximum in the winter, which is consistent with the enhanced Asian outflow to the Pacific indicated by backward air-mass trajectories. The concentration levels of levoglucosan estimated from global aerosol model outputs in the winter are, on average, comparable to the observed levels, suggesting that a considerable fraction of levoglucosan did not decompose during long-range transport from the Asian continent by westerly/northwesterly winds. This result is supported by comparable ratios of levoglucosan to EC in Chichi-jima and the East Asian coastal region. Conversely, the measured concentrations of levoglucosan in the summer were significantly lower than the modeled one. This implies a degradation of levoglucosan in the air masses that stagnated over the Pacific, although uncertainties in the model estimate may also be partly responsible for this discrepancy. One possible degradation pathway is oxidation by OH radicals; the contribution of acid-catalyzed reactions needs further investigation. 相似文献
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Yasuyuki Kitamori Michihiro Mochida Kimitaka Kawamura 《Atmospheric environment (Oxford, England : 1994)》2009,43(21):3416-3423
Aerosol water content (AWC) of urban atmospheric particles was investigated based on the hygroscopic growth measurements for 100 and 200 nm particles using a hygroscopicity tandem differential mobility analyzer in Sapporo, Japan in July 2006. In most of the humidogram measurements, presence of less and more hygroscopic mode was evident from the different dependence on relative humidity (RH). The volume of liquid water normalized by that of dry particle (Vw(RH)/Vdry) was estimated from the HTDMA data for 100 and 200 nm particles. The RH dependence of Vw(RH)/Vdry was well represented by a fitted curve with a hygroscopicity parameter κeff. The κeff values for 200 nm particles were in general higher than those for 100 nm particles, indicating a higher hygroscopicity of 200 nm particles. Based on the κeff values, the volume mixing ratios of water-soluble inorganic compounds (ammonium sulfate equivalent) were estimated to be on average 31% and 45% for 100 and 200 nm particles, respectively. The diurnal variation of κeff, with relatively higher values in the noontime and nighttime and lower values in the morning and evening hours, was observed for both particle sizes. The Vw(RH)/Vdry values under ambient RH conditions were estimated from κeff to range from 0.05 to 2.32 and 0.06 to 2.43 for 100 nm and 200 nm particles, respectively. The degree of correlation between κeff and Vw(RH)/Vdry at ambient RH suggests a significant contribution of the variation of κeff to atmospheric AWC in Sapporo. 相似文献
19.
Atmospheric concentrations of 4-oxopentanal (4-OPA) in both gas and particulate phase were measured at the experimental forest, 200 km north of Sapporo, Japan, from August 13 to 15, 2001. 4-OPA was collected using an annular denuder sampling system and measured with a gas chromatography employing benzylhydroxyl oxime derivatization. Its gas phase concentrations ranged from 180 ng m(-3) (44 pptv) to 1570 ng m(-3) (384 pptv), whereas those in the particulate phase were from below the detection limit (25 ng m(-3)) to 207 ng m(-3). The particulate 4-OPA accounted for 28% (particle/(gas+particle)) of the total concentration as the maximum at 06:00 on August 15th (average: 10%). The particulate concentrations of 4-OPA were found to be comparable to those of pinonic acid, indicating that 4-OPA is also an important constituent of organic aerosols in the forestal atmosphere. Here, we report, for the first time, the concentrations of 4-OPA in both gas and particulate phase and its diurnal variations in the forestal atmosphere. 相似文献