Sperm limitation and the evolution of extreme polyandry in honeybees (Apis mellifera L.)

Honeybee queens (Apis mellifera) show extreme levels of polyandry, but the evolutionary mechanisms underlying this behaviour are still unclear. The sperm-limitation hypothesis, which assumes that high levels of polyandry are essential to get a lifetime sperm supply for large and long-lived colonies, has been widely disregarded for honeybees because the semen of a single male is, in principle, sufficient to fill the spermatheca of a queen. However, the inefficient post-mating sperm transfer from the queens lateral oviducts into the spermatheca requires multiple matings to ensure an adequate spermatheca filling. Males of the African honeybee subspecies A. m. capensis have fewer sperm than males of the European subspecies A. m. carnica. Thus, given that sperm limitation is a cause for the evolution of multiple mating in A. mellifera, we would expect A. m. capensis queens to have higher mating frequencies than A. m. carnica. Here we show that A. m. capensis queens indeed exhibit significantly higher mating frequencies than queens of A. m. carnica, both in their native ranges and in an experiment on a North Sea island under the same environmental conditions. We conclude that honeybee queens try to achieve a minimum number of matings on their mating flights to ensure a sufficient lifetime sperm supply. It thus seems premature to reject the sperm-limitation hypothesis as a concept explaining the evolution of extreme polyandry in honeybees.Communicated by R.E. Page     

Trophodynamics and seasonal cycle of the copepod Pseudocalanus acuspes in the Central Baltic Sea (Bornholm Basin): evidence from lipid composition

Seasonal lipid dynamics of the copepod Pseudocalanus acuspes were studied in the Bornholm Basin (Central Baltic Sea) on a monthly basis from March 2002 until March 2003 and were interpreted in light of life cycle strategies and diet selection. The individual total lipid content of females ranged from 0.9 to 1.8 μg, with relative wax ester content reaching a significant maximum in May (44% of total lipids) and minimum (17% of total lipids) in April and November. Significant changes in size, lipid content, lipid classes and fatty acid composition of structural as well as storage lipids suggested five characteristic seasonal phases that were induced by different feeding histories and environmental conditions. Storage lipids were characterized by 18:1(n−9) as major component, which ranged between 44% of total fatty acids in June and 23% in February. The strong coherence between 18:1(n−9) in the seston lipids and the occurrence of ciliates emphasized the importance of ciliates in the diet of P. acuspes. As indicated by changes in the amounts of fatty acid markers, other food sources varied over the year, suggesting an opportunistic feeding behavior. The spring period was characterized by an increase in typical diatom and dinoflagellate markers, whereas other sources, potentially cyanobacteria, became more important during summer. The life cycle strategy is discussed with respect to extant adaptations to high latitudinal habitats.     

The Journal of Coastal Conservation, 1995–2004

This paper concludes the publication of theJournal of Coastal Conservation from 1995–2004 after the European Coastal Union and the Publishers decided to terminate the production of the journal. We look back at the 206 research papers published in this period while concentrating on Geographical and ecological variation, Focuses and trends, and Progress in European coastal conservation and management. Three indices are added: (1) a list of the 206 papers; (2) an Author Index; and (3) a Subject Index based on the titles, keywords and some additional important subjects found in the tables and figures.     

Local and systemic induced responses to cabbage root fly larvae (Delia radicum) in Brassica nigra and B. oleracea

Summary. Feeding by belowground herbivores may induce systemic changes in shoot defence levels that affect the performance of above ground herbivores and higher trophic levels. In this paper two wild Brassica species, B. nigra and B. oleracea were experimentally infested with 10 larvae of the cabbage root fly, Delia radicum. Plant dry masses and glucosinolate levels in shoots, main roots, and fine roots were determined at 3, 7, 12 and 14 days after infestation and compared to those of control plants. The systemic response in the leaves differed between plant species. In B. nigra shoot glucosinolate levels in D. radicum infested plants steadily increased with time until they were almost twice those of controls 14 days after infestation. B. oleracea plants infested with D. radicum did not show significant changes in shoot glucosinolate levels within 14 days, which may be due to the unexpected poorer performance of D. radicum on this species. Both plant species showed a local increase in indole glucosinolates in the main roots, which are the preferred feeding site of D. radicum larvae. B. oleracea plants however showed a stronger (1.9 – 4.7 times) increase in indole glucosinolate levels than B. nigra (1.5 – 2.6 times). The increase in indole glucosinolates in B. nigra main roots, was counterbalanced by a significant decrease in aromatic glucosinolate levels. These differences in local responses to D. radicum feeding between the two species may have contributed to the slower growth rates of the larvae on B. oleracea. D. radicum feeding did not result in altered glucosinolate levels in the fine roots in either plant species. The differences in glucosinolate induction patterns between the summer annual B. nigra and the perennial B. oleracea are discussed in the light of their different life histories.     

Why do Varroa mites invade worker brood cells of the honey bee despite lower reproductive success?

Varroa jacobsoni reproduces both in drone and worker brood cells of honey bees, but in drone cells reproductive success is higher than in worker cells. A simple model using clonal population growth as a fitness measure has been developed to study the circumstances under which specialization on drone brood would be a better strategy than reproduction in both types of cell. For European Apis mellifera, the model suggests that if mites have to wait less than 7 days on average before they can invade a drone cell, specialization on drone brood would be a better strategy. This is close to the estimated waiting time of 6 days. Hence, small differences in reproductive success in drone and worker cells and in the rate of mortality may determine whether specialization on drone brood will be promoted or not. In European A. mellifera colonies, Varroa mites invade both drone and worker cells, but specialization on drone brood cells seems to occur to some extent because drone cells are more frequently invaded than worker cells. In the parasite-host association of V. jacobsoni with African or Africanized A. mellifera or with A. cerana, the mites also invade both drone and worker cells, but the mites specialize on drone brood for reproduction since a large percentage of the mites in worker brood do not reproduce. Only in the parasite-host association of Euvarroa sinhai, a mite closely resembling V. jacobsoni, and A. forea is specialization complete, because these mites only invade drone brood.     

Comparing urban air quality in Europe in real time a review of existing air quality indices and the proposal of a common alternative

The EU directives on air quality force member states to inform the public on the status of the ambient air quality. The Internet is commonly used for this purpose and often air quality is being presented as an index ranging from good to bad. A review of existing websites and air quality indices shows that the way air quality is interpreted differs considerably. The paper presents a new air quality index. The index is part of a project to develop a website dedicated to comparing air quality in European cities. The common air quality index (CAQI) is not aimed at replacing existing local indices. The CAQI is a set of two indices: one for roadside monitoring sites and one for average city background conditions. Differentiating between roadside and general city conditions is a first step in assuring consistence in the parameters that are being compared.     

Precise sex ratio in a parasitic wasp: the result of counting eggs

Summary The parasitic wasp Nesolynx albiclavus maintains a very skewed offspring sex ratio (ca. 4% males). We studied proximate, causal factors of the son-daughter choice mechanism to obtain insight into the precision of the procedures and the underlying rules. We analyzed the number of males in clutches produced during sessions of 12 or 24 h. The results show that the timing of the next male egg depends on the time elapsed and the number of eggs laid since the previous one. The influence of the passage of time per se during non-laying periods was reduced in an experiment with 4-h periods. The data show that the first male egg is produced by a fairly rigid process: about the sixth egg in a run will be left unfertilized. No further male egg will appear in the next 10–20 eggs if they are laid in rapid succession.