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The endostyle is a glandular and ciliated groove lining the pharyngeal floor of tunicates. This paper describes the histological
structure of this organ in two new species of doliolid-like animals, Doliopsis bahamensis and Paradoliopsis harbisoni, and compares it with that of Doliolum nationalis. The results confirm the distinction between the Doliolida and the other Thaliacea.
Received: 7 November 1996 / Accepted: 6 December 1996 相似文献
364.
Allele-specific amplification for preimplantation genetic diagnosis (PGD) of spinal muscular atrophy
Céline Moutou Nathalie Gardes Catherine Rongières Jeanine Ohl Karima Bettahar-Lebugle Christiane Wittemer Pierre Gerlinger Stéphane Viville 《黑龙江环境通报》2001,21(6):498-503
We have developed a new allele-specific amplification method for the preimplantation genetic diagnosis (PGD) of spinal muscular atrophy (SMA; Werdnig-Hoffmann disease) from a single cell. This method is based on the detection of the deletion of exon 7 of the telomeric copy of the survival motor neurone (SMNt) gene. An oligonucleotide was designed to be specific to the SMNt nucleotidic sequence with exonic mismatch G (for SMNt)→A (for SMNc) at its 3′ end. This test produces reliable PCR products in 95% of single lymphoblasts (85/88) tested as well as in 16/16 blastomeres from normal controls. Specificity analysis showed that we were able to detect homozygous deletion of the SMNt gene in 99% of single lymphoblasts (103/104) from a SMA patient. No contamination was detected in 68 blanks tested. Multiple cell and DNA dilution analysis revealed that the test is accurate and specific up to 100 pg DNA and should thus also be suitable for PGD at the blastocyst stage. This rapid procedure requires a single round of fluorescent PCR and no restriction digestion, while previously described single cell methods include nested PCR followed by restriction enzyme digestion. Two PGD cycles for SMA using this procedure were performed in our centre. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
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366.
Camille Codaccioni Olivier Picone Véronique Lambert Paul Maurice Léo Pomar Norbert Winer Laurent Guibaud Rose-Anne Lavergne Anne-Hélène Saliou Dorothée Quinio Alexandra Benachi Catherine Noel Yves Ville Fabrice Cuillier Christelle Pomares Nicole Ferret Denis Filisetti Anne-Sophie Weingertner Valérie Vequeau-Goua Estelle Cateau Guillaume Benoist Martine Wallon Marc Dommergues Isabelle Villena Laurent Mandelbrot 《黑龙江环境通报》2020,40(13):1741-1752
367.
Romain Amiot Delphine Angst Serge Legendre Eric Buffetaut François Fourel Jan Adolfssen Aurore André Ana Voica Bojar Aurore Canoville Abel Barral Jean Goedert Stanislaw Halas Nao Kusuhashi Ekaterina Pestchevitskaya Kevin Rey Aurélien Royer Antônio Álamo Feitosa Saraiva Bérengère Savary-Sismondini Jean-Luc Siméon Alexandra Touzeau Zhonghe Zhou Christophe Lécuyer 《Die Naturwissenschaften》2017,104(5-6):47
Oxygen isotope compositions of bone phosphate (δ18Op) were measured in broiler chickens reared in 21 farms worldwide characterized by contrasted latitudes and local climates. These sedentary birds were raised during an approximately 3 to 4-month period, and local precipitation was the ultimate source of their drinking water. This sampling strategy allowed the relationship to be determined between the bone phosphate δ18Op values (from 9.8 to 22.5‰ V-SMOW) and the local rainfall δ18Ow values estimated from nearby IAEA/WMO stations (from ?16.0 to ?1.0‰ V-SMOW). Linear least square fitting of data provided the following isotopic fractionation equation: δ18Ow = 1.119 (±0.040) δ18Op ? 24.222 (±0.644); R 2 = 0.98. The δ18Op–δ18Ow couples of five extant mallard ducks, a common buzzard, a European herring gull, a common ostrich, and a greater rhea fall within the predicted range of the equation, indicating that the relationship established for extant chickens can also be applied to birds of various ecologies and body masses. Applied to published oxygen isotope compositions of Miocene and Pliocene penguins from Peru, this new equation computes estimates of local seawater similar to those previously calculated. Applied to the basal bird Confuciusornis from the Early Cretaceous of Northeastern China, our equation gives a slightly higher δ18Ow value compared to the previously estimated one, possibly as a result of lower body temperature. These data indicate that caution should be exercised when the relationship estimated for modern birds is applied to their basal counterparts that likely had a metabolism intermediate between that of their theropod dinosaur ancestors and that of advanced ornithurines. 相似文献
368.
Jaffrezic A Jardé E Pourcher AM Gourmelon M Caprais MP Heddadj D Cottinet P Bilal M Derrien M Marti R Mieszkin S 《Journal of environmental quality》2011,40(3):959-968
Fecal contamination of water resources is evaluated by the enumeration of the fecal coliforms and Enterococci. However, the enumeration of these indicators does not allow us to differentiate between the sources of fecal contamination. Therefore, it is important to use alternative indicators of fecal contamination to identify livestock contamination in surface waters. The concentration of fecal indicators (, enteroccoci, and F-specific bacteriophages), microbiological markers (Rum-2-bac, Pig-2-bac, and ), and chemical fingerprints (sterols and stanols and other chemical compounds analyzed by 3D-fluorescence excitation-matrix spectroscopy) were determined in runoff waters generated by an artificial rainfall simulator. Three replicate plot experiments were conducted with swine slurry and cattle manure at agronomic nitrogen application rates. Low amounts of bacterial indicators (1.9-4.7%) are released in runoff water from swine-slurry-amended soils, whereas greater amounts (1.1-28.3%) of these indicators are released in runoff water from cattle-manure-amended soils. Microbial and chemical markers from animal manure were transferred to runoff water, allowing discrimination between swine and cattle fecal contamination in the environment via runoff after manure spreading. Host-specific bacterial and chemical markers were quantified for the first time in runoff waters samples after the experimental spreading of swine slurry or cattle manure. 相似文献
369.
Sheng Fulai Gary Flomenhoft Timothy J. Downs MaÁngeles Grande‐Ortiz Dana Graef Bert Scholtens Arthur P.J. Mol David A. Sonnenfeld Gert Spaargaren Rajeev K. Goel Edward W.T. Hsieh Serban Scrieciu Reinhard Steurer Christine Polzin Genia Kostka Tiho Ancev Elke Pirgmaier Frank Boons Karl‐Henrik Robèrt Christopher Bryant Ke Zhou Surya Raj Acharya David Huberman Denis J. Sonwa Michelle Mycoo Dabo Guan Klaus Hubacek U. Rashid Sumaila Hector G. Lopez‐Ruiz G. Jason Jolley Michael L. Dougherty André Francisco Pilon Ravi Prakash Tulus Tambunan Sebastian Hermann 《Natural resources forum》2011,35(1):63-72
370.
Abdullah Fi?ne Cengiz Kuzu T��rker H��daverdi 《Environmental monitoring and assessment》2011,174(1-4):461-470
Blast-induced ground vibration is one of the most important environmental impacts of blasting operations because it may cause severe damage to structures and plants in nearby environment. Estimation of ground vibration levels induced by blasting has vital importance for restricting the environmental effects of blasting operations. Several predictor equations have been proposed by various researchers to predict ground vibration prior to blasting, but these are site specific and not generally applicable beyond the specific conditions. In this study, an attempt has been made to predict the peak particle velocity (PPV) with the help of fuzzy logic approach using parameters of distance from blast face to vibration monitoring point and charge weight per delay. The PPV and charge weight per delay were recorded for 33 blast events at various distances and used for the validation of the proposed fuzzy model. The results of the fuzzy model were also compared with the values obtained from classical regression analysis. The root mean square error estimated for fuzzy-based model was 5.31, whereas it was 11.32 for classical regression-based model. Finally, the relationship between the measured and predicted values of PPV showed that the correlation coefficient for fuzzy model (0.96) is higher than that for regression model (0.82). 相似文献