Biomonitoring in a clean and a multi-contaminated estuary based on biomarkers and chemical analyses in the endobenthic worm Nereis diversicolor

Relationships between biochemical and physiological biomarkers (acetylcholinesterase [AChE], catalase, and glutathione S-transferase [GST] activities, thiobarbituric acid reactive substances, glycogen, lipids and proteins) and accumulated concentrations of contaminants (polychlorinated biphenyls [PCBs], polycyclic aromatic hydrocarbons and metals) were examined in the keystone species Nereis diversicolor. The chemical analyses of worms and sediments allowed the designation of the Seine estuary and the Authie estuary as a polluted and relatively clean site respectively. Worms from the Seine estuary exhibited higher GST and lower AChE activities. Generally, larger worms had higher concentrations of energy reserves. Principal component analyses clearly highlighted intersite differences: in the first plan, GST activities and chemical concentrations were inversely related to concentrations of energy reserves; in the second one, PCB concentrations and AChE activity were inversely related. Depleted levels of energy reserves could be a consequence of combating toxicants and might predict effects at higher levels of biological organization. The use of GST and AChE activities and energy reserve concentrations as biomarkers is validated in the field in this keystone species.     

On-line solid phase extraction and liquid chromatography/tandem mass spectrometry to quantify pharmaceuticals, pesticides and some metabolites in wastewaters, drinking, and surface waters

A simple on-line method was developed for the analysis of pharmaceuticals, pesticides and some metabolites in drinking, surface and wastewater samples. The technique is based on the use of on-line solid-phase extraction combined with liquid chromatography electrospray tandem mass spectrometry with positive electrospray ionization (LC-ESI(PI)-MS/MS). The injection of only 1 mL of filtered water sample is used with a total analysis time of 20 min, including the period required to flush the SPE cartridge with organic solvent and reconditioning the LC column. Method detection limits were in the range of 2 to 24 ng L(-1) for the compounds of interest, with recoveries from 87 to 110% in surface as well as wastewater samples. Matrix effects were observed for some compounds without exceeding more than 25%. All results displayed a good degree of reproducibility, with relative standard deviations (RSD) of less than 12% for all compounds. Moreover, at least 200 samples were analyzed without altering the performance of the pre-concentration column. This method was preferred over traditional off-line procedures because it minimizes tedious sample preparation, increases productivity and sample throughput. The analysis of various water and wastewater samples showed that caffeine, carbamazepine and atrazine could be detected in all the samples analysed and the selected compounds are always present in at least one of the sample types.     

Dynamical Allocation Method of Emission Rights of Pollutants by Viability Constraints Under Tychastic Uncertainty

This study proposes a method of dynamic decentralization by constraints and its associated software. It can be used to allocate pollutant emissions rights among the different polluters such that they achieve both given global and local emission thresholds not to be transgressed. Knowing the maximum growth rates of polluting emissions of each polluter in the worst case, this method provides the rule of a dynamic allocation of pollutant emissions rights as well as the required initial emissions of each polluters assuring that, whatever the growth rates of the emissions below the maximum growth rates, the resulting emissions will be, both globally and locally, under their thresholds. These guaranteed initial emissions supply each polluter with a measure of risk insurance. This problem, formulated as a “tychastic” regulated system with viability constraints, is solved with mathematical and algorithmic tools of viability theory and numerical results obtained by a dedicated software are presented.     

Documentation of bioaerosol concentrations in an indoor composting facility in France

Bioaerosol concentrations were investigated in a totally indoor composting facility processing fermentable household and green wastes to assess their variability. Stationary samples were collected by filtration close to specific composting operations and then were analysed for cultivable mesophilic bacteria, thermophilic bacteria, mesophilic fungi, thermophilic fungi, endotoxins and total airborne bacteria (DAPI-staining). Indoor concentrations exceeded the background levels, between 500 and 5400 EU m(-3) for endotoxins, 10(4) and 10(6) CFU m(-3) for cultivable bacteria and generally below 10(5) CFU m(-3) for airborne cultivable fungi. No significant (p > 0.05) differences were observed between the indoor composting operations. Successive 30 minute bioaerosol samples were collected to investigate the variation of cultivable mesophilic microorganisms over the work shift. Concentrations of mesophilic bacteria and fungi varied up to 1 log unit depending on the time at which they were collected in the day. Total airborne particles, counted using an optical particle counter, were present at up to 10(8) particles m(-3) and several concentration peaks were noted. Values for total airborne bacteria were roughly 70-fold higher than cultivable bacteria. These results raise the question of the sampling strategy (duration of sampling; number of samples to be collected) used in similar studies. They provide new bioaerosol concentration data in a composting facility and suggest that the filtration sampling method might be a useful tool for exposure measurements in that occupational environment.     

Impact of metal stress on the production of secondary metabolites in <Emphasis Type="Italic">Pteris vittata</Emphasis> L. and associated rhizosphere bacterial communities

Plants adapt to metal stress by modifying their metabolism including the production of secondary metabolites in plant tissues. Such changes may impact the diversity and functions of plant associated microbial communities. Our study aimed to evaluate the influence of metals on the secondary metabolism of plants and the indirect impact on rhizosphere bacterial communities. We then compared the secondary metabolites of the hyperaccumulator Pteris vittata L. collected from a contaminated mining site to a non-contaminated site in Vietnam and identified the discriminant metabolites. Our data showed a significant increase in chlorogenic acid derivatives and A-type procyanidin in plant roots at the contaminated site. We hypothesized that the intensive production of these compounds could be part of the antioxidant defense mechanism in response to metals. In parallel, the structure and diversity of bulk soil and rhizosphere communities was studied using high-throughput sequencing. The results showed strong differences in bacterial composition, characterized by the dominance of Proteobacteria and Nitrospira in the contaminated bulk soil, and the enrichment of some potential human pathogens, i.e., Acinetobacter, Mycobacterium, and Cupriavidus in P. vittata’s rhizosphere at the mining site. Overall, metal pollution modified the production of P. vittata secondary metabolites and altered the diversity and structure of bacterial communities. Further investigations are needed to understand whether the plant recruits specific bacteria to adapt to metal stress.     

Value of biochars from Miscanthus x giganteus cultivated on contaminated soils to decrease the availability of metals in multicontaminated aqueous solutions

The objective of this study was to evaluate the sorption efficiency of eight biochars, made from Miscanthus x giganteus cultivated on contaminated agricultural soil, in aqueous solutions contaminated with metals alone or mixed with polycyclic aromatic hydrocarbons. These biochars were produced in different pyrolysis conditions (temperature, 400/600 °C; heating rate, 5/10 °C min⁻¹; duration, 45/90 min) and compared with an uncontaminated commercialized biochar made of wood. The physicochemical characterization of the Miscanthus biochars confirmed the impact of the pyrolysis on the biochar parameters with substantial differences between the biochars in terms of pH, cation exchange capacity, and specific surface area. The sorption experiment showed higher sorption efficiency of Cd, Pb, and Zn for the Miscanthus biochars produced at 600 °C compared with the biochars produced at 400 °C when the aqueous solutions were mono- or multicontaminated. Furthermore, the desorption study showed that the sorption process was largely irreversible. Therefore, the high sorption capacity of Miscanthus biochars and the low sorption reversibility confirmed that these biochars are a suitable sorbent for metals.

     

Analysing the influence of different street vegetation on traffic-induced particle dispersion using microscale simulations

Urban vegetation can be viewed as compensation to the environmental drawbacks of urbanisation. However, its ecosystem function is not well-known and, for urban planning, vegetation is mainly considered as an element of urban design. This article argues that planning practice needs to re-examine the impact of vegetation cover in the urban fabric given our evaluation of vegetation's effects on air quality, including the dispersion of traffic-induced particles at street level. Using the three-dimensional microclimate model ENVI-met?, we evaluate these effects regarding the height-to-width ratio of streets flanked by buildings and the vertical and horizontal density of street vegetation. Our results reveal vegetation's effect on particle dispersion through its influence on street ventilation. In general, vegetation was found to reduce wind speed, causing inhibition of canyon ventilation and, consequently, an increase in particle concentrations. Vegetation was also found to reduce wind speed at crown-height and to disrupt the flow field in close vicinity to the canopy. With increasing height-to-width ratio of street canyons, wind speed reduction increases and the disturbance of the flow impacts across a canyon's entire width. We also found that the effect is more pronounced in configurations with poor ventilation, such as the low wind speed, perpendicular inflow direction, and in deep canyons cases.     

Carbon and nitrogen stocks and nitrogen mineralization in organically managed soils amended with composted manures

The use of composted manures and of legumes in crop rotations may control the quality and quantity of soil organic matter and may affect nutrient retention and recycling. We studied soil organic C and N stocks and N mineralization in organically and conventionally managed dryland arable soils. We selected 13 extensive organic fields managed organically for 10 yr or more as well as adjacent fields managed conventionally. Organic farmers applied composted manures ranging from 0 to 1380 kg C ha yr and incorporated legumes in crop rotations. In contrast, conventional farmers applied fresh manures combined with slurries and/or mineral fertilizers ranging from 200 to 1900 kg C ha yr and practiced a cereal monoculture. Despite the fact that the application of organic C was similar in both farming systems, organically managed soils showed higher C and similar N content and lower bulk density than conventionally managed soils. Moreover, organic C stocks responded to the inputs of organic C in manures and to the presence of legumes only in organically managed soils. In contrast, stocks of organic N increased with the inputs of N or C in both farming systems. In organically managed soils, organic N stocks were less mineralizable than in conventional soils. However, N mineralization in organic soils was sensitive to the N fixation rates of legumes and to application rate and C/N ratio of the organic fertilizers.