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Evaluating seasonal dynamics of bacterial communities in marine fish aquaculture: a preliminary study before applying phage therapy 总被引:1,自引:0,他引:1
Pereira C Salvador S Arrojado C Silva Y Santos AL Cunha A Gomes NC Gomes N Almeida A 《Journal of environmental monitoring : JEM》2011,13(4):1053-1058
The increasing problem of antibiotic resistance in common pathogenic bacteria and the concern about the spreading of antibiotics in the environment bring the need to find new methods to control fish pathogens. Phage therapy represents a potential alternative to antibiotics, but its use in aquaculture requires a detailed understanding of bacterial communities, namely of fish pathogenic bacteria. Therefore, in this study the seasonal dynamics of the overall bacterial communities, microbiological water quality and disease-causing bacteria were followed in a marine aquaculture system of Ria de Aveiro (Portugal). Analysis of the bacterial diversity of the water samples by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments indicates that the bacterial community structure varied seasonally, showing a higher complexity during the warm season. The diversity of the main fish pathogenic bacteria, assessed by DGGE targeting the Vibrio genus, showed lower seasonal variation, with new dominating populations appearing mainly in the spring. Bacterial indicators, faecal coliforms and enterococci, enumerated by the filter-membrane method, also varied seasonally. The fluorescent in situ hybridization (FISH) results showed that the specific groups of bacteria varied during the study period and that the non-indigenous Enterobactereaceae family was the most abundant group followed by Vibrio and Aeromonas. The seasonal variation detected in terms of density and structure of total and pathogenic bacterial communities demonstrates the need for a careful monitoring of water through the year in order to select the suitable phages to inactivate fish pathogenic bacteria. The spring season seems to be the critical time period when phage therapy should be applied. 相似文献
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Simon Lüderwald Kymberly Newton Katharina Heye Kristina Bitter Samuel Moeris Lena Benner Paul Böhm Josef Koch Alexander Feckler Mafalda Castro Andreas Erikkson 《Environmental Sciences Europe》2018,30(1):41
This commentary is an introduction for students to the Society of Environmental Toxicology and Chemistry (SETAC) and its Student Advisory Council (SAC). As young academics face challenges while trying to develop their careers, SETAC and the SAC help facilitate student involvement in the various communities within the society that can help to develop the students’ careers within the environmental sciences [e.g. the German Language Branch (GLB)]. This piece would also like to emphasize and pay homage to the continual cooperation between the SAC and the ESEU, which provides a scientific platform to communicate internationally and beyond the borders of SETAC, as well as offer heartfelt congratulations from the SAC to the GLB for their “20 Years SETAC GLB” and deep gratitude for their strong advocacy and support of the SAC. 相似文献
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The relationship between residence time and oxygen saturation was investigated in a mesotidal lagoon in southern Portugal. The system receives no significant freshwater input during the summer months and has a high evaporation rate. These features enable an estimate of residence time from the salinity differences between ocean water entering the system and lagoon water. More than 10,000 GPS referenced measurements of oxygen saturation, temperature and salinity were made during large spring tides in September, 2006. The lowest oxygen saturation ( approximately 44%) was measured in the waters with the highest calculated residence times (7 days). There was a significant linear decrease in the oxygen saturation with increasing residence time of approximately 16% per day. This was similar to the rate measured on a neap tide in August, 2005. The high salinity, low oxygen saturated water was spatially confined to one inner channel, except at high water when this water was pushed into other channels. Although the tides investigated were the largest for several years, the oxygen saturation did not exceed 70% in this inner region. It is proposed that the direct discharge of oxygen consuming effluent, including domestic sewage, into this inner channel is responsible for this persistent oxygen deficit. 相似文献
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KeithWorrall AnthonyNewton 《环境化学》2005,24(6):739-740
在现代实验室中,GC/MS用于痕量分析极为普遍,要求更低的检测限而被分析样品的基体越来越复杂的情况由来已久.…… 相似文献