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Oils and grease (O&G) have low affinity for water and represent a class of pollutants present in the dairy industry. Enzyme-mediated bioremediation using biocatalysts, such as lipases, has shown promising potential in biotechnology, as they are versatile catalysts with high enantioselectivity and regioselectivity and easy availability, being considered a clean technology (white biotechnology). Specially in the treatment of effluents from dairy industries, these enzymes are of particular importance as they specifically hydrolyze O&G. In this context, the objective of this work is to prospect filamentous fungi with the ability to synthesize lipases for application in a high-fat dairy wastewater environment. We identified and characterized the fungal species Aspergillus sclerotiorum as a good lipase producer. Specifically, we observed highest lipolytic activity (20.72 U g−1) after 96 h of fermentation using sunflower seed as substrate. The fungal solid fermented was used in the bioremediation in dairy effluent to reduce O&G. The experiment was done in kinetic from 24 to 168 h and reduced over 90% of the O&G present in the sample after 168 h. Collectively, our work demonstrated the efficiency and applicability of fungal fermented solids in bioremediation and how this process can contribute to a more sustainable wastewater pretreatment, reducing the generation of effluents produced by dairy industries.
相似文献When performed without technical criteria, the rapid expansion of irrigated agricultural frontiers can result in overexploitation of water, causing worrying impacts on the balance of agroecosystems. This study proposes a model applied to the state of Bahia, to estimate the water demand of areas irrigated by a central pivot, in order to contribute to information that will subsidize the inspection and planning of water resources in the promotion of sustainable agriculture. The irrigated areas were identified and measured by photointerpretation using orbital images from the Landsat-8 satellite. With a historical series of data, the reference evapotranspiration was calculated and monthly water balance was elaborated. The data obtained were spatialized by kriging, and with punctual values of water deficit (mm), the water demand of the irrigated perimeter of the equipment was estimated. The results were described considering strategic planning units, proposed from municipalities, hydrographic basins and biomes. A total of 4075 pivots were quantified, covering an irrigated area of 265,896.30 ha and with an average annual consumption of 1,333,473,208.02 m3 of water. Areas of high demand were identified, especially in the western region of Bahia, which includes the hydrographic basin of the São Francisco River and the Cerrado biome, concentrating 80.85% and 75.47% of the state water demand for pivots, respectively. Considering possible points of water vulnerability and continuity of this expansion, the results provide the primary information needed to encourage the adoption of public policies aimed at the management of water resources. The study method proposes guidelines that condition the application in any region of interest in the world.
Graphical abstract 相似文献