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291.
Helen Carnaghan Catherine P. James Paul B. Charlesworth Marco Ghionzoli Susana Pereira Mohamed Elkhouli David Baud Paolo De Coppi Greg Ryan Prakesh S. Shah Mark Davenport Anna L. David Agostino Pierro Simon Eaton Gastroschisis Study Group 《黑龙江环境通报》2020,40(8):991-997
Objective
In gastroschisis, there is evidence to suggest that gut dysfunction develops secondary to bowel inflammation; we aimed to evaluate the effect of maternal antenatal corticosteroids administered for obstetric reasons on time to full enteral feeds in a multicenter cohort study of gastroschisis infants.Methods
A three center, retrospective cohort study (1992-2013) with linked fetal/neonatal gastroschisis data was conducted. The primary outcome measure was time to full enteral feeds (a surrogate measure for bowel function) and secondary outcome measure was length of hospital stay. Analysis included Mann-Whitney and Cox regression.Results
Of 500 patients included in the study, 69 (GA at birth 34 [25-38] weeks) received antenatal corticosteroids and 431 (GA at birth 37 [31-41] weeks) did not. Antenatal corticosteroids had no effect on the rate of reaching full feeds (Hazard ratio HR 1.0 [95% CI: 0.8-1.4]). However, complex gastroschisis (HR 0.3 [95% CI: 0.2-0.4]) was associated with an increased time to reach full feeds and later GA at birth (HR 1.1 per week increase in GA [95% CI: 1.1-1.2]) was associated with a decreased time to reach full feeds.Conclusion
Maternal antenatal corticosteroids use, under current antenatal steroid protocols, in gastroschisis is not associated with an improvement in neonatal outcomes such as time to full enteral feeds or length of hospital stay. 相似文献292.
Marco Ostoich Emilia Aimo Rita Frate Marina Vazzoler Silvia Stradella Paolo Osti 《Chemistry and Ecology》2007,23(1):43-62
Wastewaters are a source of pathogenic micro-organisms in the environment. The microbial load and residues found in the final effluents of wastewater-treatment plants (WWTPs) depend on the WWTPs' abatement capacity and the final disinfection treatment systems applied to wastewaters before discharge into water. A historical database with data on surface and marine-coastal water quality and on the characterization of WWTP effluents was made using data from 1997 to 2004 to assess the microbiologic impact along the coast of the Venice province (Italy, northern Adriatic sea). The monitoring of river and sea discharges along the coast is integrated with the application of the Synthetic Pluriennal Faecalization Index (ISPF). The experimental study was conducted in the period from November 2002 to April 2004 by the Veneto Regional Environmental Prevention and Protection Agency. The results of this investigation on faecal contamination together with previous data are presented with a preliminary performance characterization of the WWTPs' disinfection technologies (sodium hypochlorite, peracetic acid, UV rays, and ozone). 相似文献
293.
Chingoileima Maibam Patrick Fink Giovanna Romano Maria Cristina Buia Maria Cristina Gambi Maria Beatrice Scipione Francesco Paolo Patti Maurizio Lorenti Emanuela Butera Valerio Zupo 《Marine Biology》2014,161(7):1639-1652
Plants evolve the production of wound-activated compounds (WACs) to reduce grazing pressure. In addition, several plant-produced WACs are recognized by various invertebrates, playing the role of infochemicals. Due to co-evolutionary processes, some invertebrates recognize plant infochemicals and use them to identify possible prey, detect the presence of predators or identify algae containing various classes of toxic metabolites. Different metabolites present in the same algae can play the role of toxins, infochemicals or both simultaneously. We investigated the infochemical activity of compounds extracted from three diatoms epiphytes of the seagrass Posidonia oceanica, by conducting choice experiments on invertebrates living in the same community or in close proximity. Furthermore, the specific toxicity of the extracts obtained from the same algae was tested on sea urchin embryos using a standard bioassay procedure, to detect the presence of toxins. The comparison of the two effects demonstrated that invertebrates are subjected to diatom wound-activated toxicants when these algae are not associated with their own habitat, but they are able to recognize volatile infochemicals derived from diatoms associated with their habitats. The specific toxicity of WACs was shown to be inversely correlated to the perceptive ability of invertebrates towards volatile compounds liberated by the same algae. Hence, when the recognition of specific algae by a given invertebrate species evolves, their detrimental effects on the receiving organism may be lost. 相似文献
294.
Paolo Magni 《Chemistry and Ecology》2013,29(5):363-372
Coastal marine ecosystems are increasingly subjected to environmental stress and degradation due to pollution. Several research programmes have addressed this problem and produced relevant data sets for specific areas, often including consistent sets of environmental and biological variables. The value of existing information gathered from these types of data can be largely increased by combining them into a common data set to determine globally applicable relationships. To perform this exercise, the Intergovernmental Oceanographic Commission (IOC) of UNESCO has recently formed the Ad hoc Study Group on Benthic Indicators (http://www.ioc.unesco.org/benthicindicators) with the aim of developing robust indicators of benthic health. In this paper, initial products and ongoing activities of this international initiative are described and discussed. An expansion of initial IOC/UNESCO research on benthic fauna-organic carbon relationships is also presented. As part of this follow-up research, the relationship between total organic carbon concentrations of sediment and abundance, biomass and species diversity of benthic macrofauna was evaluated using data sets from 2 different regions of the world comprising 3 different coastal marine environments. The ability of identifying threshold levels in selected variables that could serve as indicators of related adverse environmental conditions leading to stress in the benthos is envisaged within the frame of a larger joint analysis, carried out by the IOC/UNESCO Study Group on Benthic Indicators, of merged data sets from several coastal regions worldwide. 相似文献
295.
Bolzonella D Cavinato C Fatone F Pavan P Cecchi F 《Waste management (New York, N.Y.)》2012,32(6):1196-1201
The paper reports the findings of a two-year pilot scale experimental trial for the mesophilic (35°C), thermophilic (55°C) and temperature phased (65+55°C) anaerobic digestion of waste activated sludge. During the mesophilic and thermophilic runs, the reactor operated at an organic loading rate of 2.2 kgVS/m(3)d and a hydraulic retention time of 20 days. In the temperature phased run, the first reactor operated at an organic loading rate of 15 kgVS/m(3)d and a hydraulic retention time of 2 days while the second reactor operated at an organic loading rate of 2.2 kgVS/m(3)d and a hydraulic retention time of 18 days (20 days for the whole temperature phased system). The performance of the reactor improved with increases in temperature. The COD removal increased from 35% in mesophilic conditions, to 45% in thermophilic conditions, and 55% in the two stage temperature phased system. As a consequence, the specific biogas production increased from 0.33 to 0.45 and to 0.49 m(3)/kgVS(fed) at 35, 55, and 65+55°C, respectively. The extreme thermophilic reactor working at 65°C showed a high hydrolytic capability and a specific yield of 0.33 g COD (soluble) per gVS(fed). The effluent of the extreme thermophilic reactor showed an average concentration of soluble COD and volatile fatty acids of 20 and 9 g/l, respectively. Acetic and propionic acids were the main compounds found in the acids mixture. Because of the improved digestion efficiency, organic nitrogen and phosphorus were solubilised in the bulk. Their concentration, however, did not increase as expected because of the formation of salts of hydroxyapatite and struvite inside the reactor. 相似文献
296.
J. B. Legradi C. Di Paolo M. H. S. Kraak H. G. van der Geest E. L. Schymanski A. J. Williams M. M. L. Dingemans R. Massei W. Brack X. Cousin M.-L. Begout R. van der Oost A. Carion V. Suarez-Ulloa F. Silvestre B. I. Escher M. Engwall G. Nilén S. H. Keiter D. Pollet P. Waldmann C. Kienle I. Werner A.-C. Haigis D. Knapen L. Vergauwen M. Spehr W. Schulz W. Busch D. Leuthold S. Scholz C. M. vom Berg N. Basu C. A. Murphy A. Lampert J. Kuckelkorn T. Grummt H. Hollert 《Environmental Sciences Europe》2018,30(1):46
The numbers of potential neurotoxicants in the environment are raising and pose a great risk for humans and the environment. Currently neurotoxicity assessment is mostly performed to predict and prevent harm to human populations. Despite all the efforts invested in the last years in developing novel in vitro or in silico test systems, in vivo tests with rodents are still the only accepted test for neurotoxicity risk assessment in Europe. Despite an increasing number of reports of species showing altered behaviour, neurotoxicity assessment for species in the environment is not required and therefore mostly not performed. Considering the increasing numbers of environmental contaminants with potential neurotoxic potential, eco-neurotoxicity should be also considered in risk assessment. In order to do so novel test systems are needed that can cope with species differences within ecosystems. In the field, online-biomonitoring systems using behavioural information could be used to detect neurotoxic effects and effect-directed analyses could be applied to identify the neurotoxicants causing the effect. Additionally, toxic pressure calculations in combination with mixture modelling could use environmental chemical monitoring data to predict adverse effects and prioritize pollutants for laboratory testing. Cheminformatics based on computational toxicological data from in vitro and in vivo studies could help to identify potential neurotoxicants. An array of in vitro assays covering different modes of action could be applied to screen compounds for neurotoxicity. The selection of in vitro assays could be guided by AOPs relevant for eco-neurotoxicity. In order to be able to perform risk assessment for eco-neurotoxicity, methods need to focus on the most sensitive species in an ecosystem. A test battery using species from different trophic levels might be the best approach. To implement eco-neurotoxicity assessment into European risk assessment, cheminformatics and in vitro screening tests could be used as first approach to identify eco-neurotoxic pollutants. In a second step, a small species test battery could be applied to assess the risks of ecosystems. 相似文献
297.
Francesco Colloca Paolo Carpentieri Elena Balestri Giandomenico Ardizzone 《Marine Biology》2010,157(3):565-574
We investigated the effects of body size, feeding strategy and depth distribution on the trophic resource partitioning among
the 26 dominant fish consumers in a fish assemblage on the central Mediterranean shelf-break. The fish assemblage was structured
in two major trophic guilds: epibenthic and zooplanktonic feeders, according to the position of each predator along the benthos–plankton
gradient. Within each main guild, the species were segregated along a prey-size or fish-size gradient into five further guilds.
Fish size and prey size were strongly correlated, indicating that the prey-size niche can be well explained by predator size.
Fish consumers showed a significant negative correlation between the similarity in prey type and the similarity in depth distribution;
most species with similar trophic preferences segregated along the depth dimension. The only predators overlapping in both
food and depth preferences were those with a more specialist trophic behavior. These results suggest that fish body size and
depth preferences are the two main niche dimensions, explaining a large part of the coexistence between the Mediterranean
shelf-break fish consumers. 相似文献
298.
Quantitative PCR to estimate copepod feeding 总被引:1,自引:0,他引:1
Jens C. Nejstgaard Marc E. Frischer Paolo Simonelli Christofer Troedsson Markus Brakel Filiz Adiyaman Andrey F. Sazhin L. Felipe Artigas 《Marine Biology》2008,153(4):565-577
Copepods play a central role in marine food webs as grazers of plankton and as key prey for many predators. Therefore, quantifying
their specific trophic interactions is critical for understanding the role of copepods in ocean processes. However, because
of methodological constraints, it remains difficult to investigate in situ copepod feeding without reliance on laborious intrusive
and potentially biased incubation approaches. Recent advances in PCR-based methodologies have demonstrated the feasibility
of directly identifying copepod diets based on prey DNA sequences. Yet, obtaining quantitative information from these approaches
remains challenging. This study presents results of systematic efforts to develop a quantitative PCR (qPCR) assay targeted
to 18S rRNA gene fragments to estimate copepod gut content of specific species of prey algae. These results were first compared
to gut content estimates based on fluorescence in the copepod Calanus finmarchicus fed monocultures of two different microalgae species in controlled laboratory studies. In subsequent field studies, we compared
feeding rates obtained by microscopy and qPCR for Temora longicornis and Acartia clausi feeding on the haptophyte Phaeocystis globosa in natural blooms. These investigations demonstrate a semi-quantitative relationship between gut content estimates derived from qPCR, gut pigment,
and direct microscopy. However, absolute estimates of gut content based on qPCR methodology were consistently lower than expected.
This did not appear to be explained by the extraction methods used, or interference by non-target (predator) DNA in the PCR
reactions, instead suggesting digestion of prey-specific nucleic acids. Furthermore, the 18S rDNA target gene copy number
of the phytoplankton varied with growth phase. Nonetheless, when prey target gene copy number in the ambient water is quantified,
the qPCR-approach can be compared to other methods, and then used to semi-quantitatively estimate relative copepod grazing
on specific prey in situ without involving further incubations. A distinct advantage of a DNA-based molecular approach compared
to gut fluorescence and direct microscopic observation, is the ability to detect non-pigmented and macerated prey. Future
studies should aim to correct for breakdown in prey DNA and perform extensive calibrations to other methods in order to achieve
a quantitative measure of feeding rates in situ. 相似文献
299.
Marino Martini Luciano Giannini Antonella Buccianti Laura Maggi Franco Prati Paola Cellini Legittimo Paolo Iozzelli Bruno Capaccioni 《Chemistry and Ecology》1991,5(3):139-147
A major influence on the environment of active volcanic areas is expected from continuous input of chemical species pertaining to fumarolic activities occurring during inter-eruptive intervals.
The systematic investigations carried out during thirteen years in the island of Vulcano (Italy) showed also substantial contributions to phreatic waters and soils of minor constituents and trace metals from volcanic rocks with no negligible influence on growing crops.
Greater extents of these phenomena have been observed for products of hydromagmatic volcanic activity or of long-lasting weathering processes. 相似文献
The systematic investigations carried out during thirteen years in the island of Vulcano (Italy) showed also substantial contributions to phreatic waters and soils of minor constituents and trace metals from volcanic rocks with no negligible influence on growing crops.
Greater extents of these phenomena have been observed for products of hydromagmatic volcanic activity or of long-lasting weathering processes. 相似文献
300.