Occurrence of polybrominated diphenyl ethers (PBDEs) in brown trout bile and liver from Swiss rivers

The ranges of total polybrominated diphenyl ethers (PBDEs) in fish from four Swiss rivers were 0.8-240 ng/g in the bile and 16-7400 ng/g lipid in the liver. PBDE concentrations varied within each river and among the various rivers. Female fish tended to have higher concentrations in the liver, while the male fish had higher concentrations in the bile. From the resulting PBDE concentrations in fish it could not be infered that these contaminants contribute to the causes of the observed fish catch decline in Swiss rivers.     

Realising a resilient and sustainable built environment: towards a strategic agenda for the United Kingdom

Recent natural and human-induced emergencies have highlighted the vulnerability of the built environment. Although most emergency events are not entirely unexpected, and the effects can be mitigated, emergency managers in the United Kingdom have not played a sufficiently proactive role in the mitigation of such events. If a resilient and sustainable built environment is to be achieved, emergency management should be more proactive and receive greater input from the stakeholders responsible for the planning, design, construction and operation of the built environment. This paper highlights the need for emergency management to take a more systematic approach to hazard mitigation by integrating more with professions from the construction sector. In particular, design changes may have to be considered, critical infrastructures must be protected, planning policies should be reviewed, and resilient and sustainable agendas adopted by all stakeholders.     

Evaluation of the Contribution of Genetic Research to the Management of the Endangered Plant Zieria prostrata

Abstract: Zieria prostrata (Rutaceae) is known from only four headlands within a 3-km stretch of coastline in New South Wales, Australia. The species was presumed to have occurred at a headland 24 km south of its present range. We used random amplified polymorphic DNA analysis to assess patterns of genetic variation within and among the extant populations. The analysis also included an individual reputedly rescued from the now extinct population. A high level of population divergence was revealed by principal coordinate analysis and an analysis of molecular variance (AMOVA; 37% among populations). Our genetic findings provide implications for the conservation management of the species. First, the loss of any one population would lead to a severe loss of genetic variation. Second, an adequate ex situ collection must sample the full range of genetic diversity from all populations. Third, the consequences of mixing populations may be an important conservation consideration if further translocations proceed. Fourth, the individual apparently sampled prior to its population extinction is genetically similar to individuals from one of the extant sites. This degree of similarity was unexpected and, after further investigation, led to the conclusion that prior existence of the species at the site is doubtful. Subsequently, a planned reintroduction program was abandoned. So far, of these four management implications, only the last has had a direct management outcome. Those implications that failed to lead to practical management outcomes did so because the same management recommendations could be obtained without genetic research. Clearly, the challenge for more effective conservation is to identify those cases in which genetic studies are likely to produce practical outcomes for conservation managers. This may be best achieved by assessing the outcomes of genetic studies already conducted.     

Evaluation of the Impact of Acid Mine Drainage on the Chemistry and the Macrobenthos in the Carolina Stream (San Luis – Argentina)

The purpose of this study was to evaluate the impact of acid mine drainage on the chemistry and the macrobenthos of the Carolina stream (San Luis – Argentina). Samples were obtained in the years 1997–1998 at two sites: site C₁, located 200 m upstream of the drainage, and site C₂, located 800 m downstream. The system buffer capacity was evaluated in the non – contaminated site by means of the buffer index calculation. The physico – chemical changes observed as a consequence of the contribution of acid mine drainage (AMD) were: a decreasing of pH and an increase in the ionic concentration, especially sulfate and Fe coming from the oxidation produced by chemiolithotrophic bacteria. The values obtained indicated a low buffer capacity and a high intrinsic vulnerability of the system to resist the impact originated by the AMD, producing a remarkable decreasing of pH of the receiving stream. These changes caused modifications in the original benthic community that was replaced by organisms more tolerant to the acid stress. A reduction in the abundance and in the taxonomic richness of the benthic macroinvertebrates was observed when compared with the reference station. An increase in the proportion of Chironomidae and of Acari and a decrease in the proportion of the remaining taxa were observed. The most sensitive groups were Ephemeroptera, Trichoptera and Mollusca. The community was mostly affected by the following variables: pH, conductivity, sulfate and dissolved total Fe.     

Spatial and Temporal Trends of Physicochemical Parameters in the Water of the Reconquista River (Buenos Aires, Argentina)

The Reconquista river is one of the most polluted watercourses in Argentina. More than 3 million people and over 10,000 industries are settled on its basin. The available data show that pollution is mainly related to the discharge of domestic and industrial liquid wastes that are poured into the river almost untreated. At present no site of the river can be characterized as free of pollutants. Samples were taken monthly from 5 sites of the river; some 18–20 physicochemical parameters were determined in each sample. Analyses revealed significant differences in the degree of deterioration between sites. Two of them, close to the source of the river (Cascallares-S₁ and Paso del Rey-S₂) resulted less polluted than the two points located close to the mouth of the river (San Martin-S₄ and Bancalari-S₅). The worsening of the water quality in S₄–S₅ was attributed to the discharges of the Moron stream, a tributary that flows into the main course of the river a complex mixture of non treated waste waters. PCA was used in the ordination of samples (sites, season and physicochemical parameters). In the PCA performed using all variables, the first principal component showed positive correlation with N-NH₄ ⁺, conductivity, orthophosphate, BOD₅, COD and alkalinity, and negative correlation with DO. The second principal component was positively correlated with pH, temperature and chlorophyll a and negatively with phenols and hardness. In respect to the spatial distribution, the plot of the scores for the first two components of samples taken in each sampling station showed S₁ and S₂ values displayed farthest at the left side of the X axis with high DO. In contrast, S₄ and S₅ values stayed at the right side of this axis with high N-NH₄ ⁺, conductivity, orthophosphate, BOD₅, COD and alkalinity; data of S₃ were “intermediate". In order to identify seasonal trends in the concentration of contaminants scores of cases labeled by season were plotted. The line drawn on the ordination plane showed that summer samples tended to converge to the upper right portion of the graph where pollution variables had more importance on the first axis, and along the second axis with high correlation with pH, temperature and chlorophyll a. Winter scores were settled in the left lower part of the plot with minor contribution of pollution parameters and more importance of DO on the first axis. Samples of autumn and spring did not fit a clear cut pattern. Deceased     

A comparison of the comprehensive municipal solid waste management systems of the region of Prague and the principality of Asturias. the influence of collection systems and municipality size on the waste collection and the recycling rates

Journal of Material Cycles and Waste Management - The correct management of municipal solid waste (MSW) is a critical factor in the protection of the environment, as well as a source for the...     

Detection of presumed genes encoding beta-lactamases by sequence based screening of metagenomes derived from Antarctic microbial mats

• Beta-lactamase genes were found in all samples from distant places in Antarctica. • Class C beta-lactamase coding genes were the most frequently found. • Diversity of sequences exceeds that of the beta-lactamases from clinical environment. Analysis of environmental samples for bacterial antibiotic resistance genes may have different objectives and analysis strategies. In some cases, the purpose was to study diversity and evolution of genes that could be grouped within a mechanism of antibiotic resistance. Different protocols have been designed for detection and confirmation that a functional gene was found. In this study, we present a sequence-based screening of candidate genes encoding beta-lactamases in 14 metagenomes of Antarctic microbial mats. The samples were obtained from different sites, representing diverse biogeographic regions of maritime and continental Antarctica. A protocol was designed based on generation of Hidden Markov Models from the four beta-lactamase classes by Ambler classification, using sequences from the Comprehensive Antibiotic Resistance Database (CARD). The models were used as queries for metagenome analysis and recovered contigs were subsequently annotated using RAST. According to our analysis, 14 metagenomes analyzed contain A, B and C beta-lactamase genes. Class D genes, however, were identified in 11 metagenomes. The most abundant was class C (46.8%), followed by classes B (35.5%), A (14.2%) and D (3.5%). A considerable number of sequences formed clusters which included, in some cases, contigs from different metagenomes. These assemblies are clearly separated from reference clusters, previously identified using CARD beta-lactamase sequences. While bacterial antibiotic resistance is a major challenge of public health worldwide, our results suggest that environmental diversity of beta-lactamase genes is higher than that currently reported, although this should be complemented with gene function analysis.     

Moderate stoichiometric homeostasis in the sea urchin Lytechinus variegatus: effects of diet and growth on C:N:P ratios

The influence of dietary elemental contents on consumer stoichiometry was investigated in selected and combined soft tissues (as a proxy of the whole individual) of the omnivorous sea urchin, Lytechinus variegatus. We raised urchins for 4 months in controlled seawater tanks using three different diets with different nutritional contents (from lower to higher: seagrass, red macroalgae, and a formulated diet). Individuals fed the different diets varied an average of 19.7, 19.4, and 38 % in C:N, C:P, and N:P ratios, respectively, with stronger temporal variability for C:P and N:P ratios across tissues and whole individuals. This resulted in homeostasis parameters (1/H) of ?0.45, 0.09, and 0.38, respectively, for C:N, C:P, and N:P, indicative of homeostatic to weakly homeostatic organisms, at least for C:P and N:P ratios. Individuals fed the nutrient-rich formulated diet had higher growth rates (14 ± 0.83 g WW month^?1) than those fed macroalgae or seagrass (9.3 ± 0.57 and 3.4 ± 0.33 g WW month^?1, respectively). However, rapid body increments in more nutritional diets caused both a decrease in the %N and an increase in the %P of soft tissues, which resulted in significant but opposite effects of diet stoichiometry and growth in sea urchin C:N (R = ?0.74 and R = 0.93, for diet and growth effects, respectively) and N:P ratios (R = 0.60 and R = ?0.63, also, respectively, for diet and growth effects). Among potential compensatory mechanisms helping to preserve certain levels of homeostasis, ingestion rates (g WW diet per g WW of urchin) were higher for seagrass and macroalgae diets than for the nutrient-rich formulated diet. In contrast, absorption and growth efficiencies displayed significant negative associations with nutrient contents in diets and did not exhibit nutritional compensation. Overall, our results suggest that resource stoichiometry strongly determines the growth rate of individuals (R = 0.88, P < 0.01), and moderate variability in C:N:P ratios of sea urchins possibly arise from differences in the allocation of proteins and RNA to body components, similarly to what has been proposed by the growth rate hypothesis.     

Switching to Plan B: changes in the escape tactics of two grasshopper species (Acrididae: Orthoptera) in response to repeated predatory approaches

Most studies examining escape behaviour have considered single approaches and single fleeing responses; few have considered how organisms’ response is influenced by persistent pursuit. We explored fleeing behaviour of two grasshopper species to test whether they modified escape behaviour when approached repeatedly. Schistocerca alutacea did not increase flight initiation distance (FID) upon repeated approach but fled farther. Psinidia fenestralis increased its FID on the second approach but decreased its flight distance over successive escapes. Both species showed a bimodal pattern of flight direction, either flying directly away or flying perpendicular to the direction of the observer’s approach. Neither species showed a significant pattern of flight direction or change in flight direction with successive escapes. Most (88 %) P. fenestralis initially landed on sand, but after repeated approaches an increasing proportion landed in grass and hid. Both species therefore changed escape behaviour with persistent pursuit but used different tactics, suiting their flight ability or camouflage, and optimised habitat use. Three grasshopper species have now been examined for responses to repeated approach by predators and all show different tactics supporting escape decision theory. Our results emphasise the variety of escape responses across species and how the dynamic nature of escape responses vary according to an animal’s situation. Rather than single optimum escape options, each grasshopper species shows a range of responses, which vary with risk from persistent predators. Although grasshoppers provide an excellent model, it would be profitable to examine responses of a range of species according to levels of predation risk.