Uptake and transformation of phenol and chlorophenols by hairy root cultures of Daucus carota, Ipomoea batatas and Solanum aviculare

Hairy root cultures of Daucus carota L., Ipomoea batatas L. and Solanum aviculare Forst were investigated for their susceptibility to the highly toxic pollutants phenol and chlorophenols and for the involvement of inherent peroxidases in the removal of phenols from liquid media. Roots of D. carota grew normally in medium containing 1000 micromol l(-1) of phenol, whilst normal growth of roots of I. batatas and S. aviculare was only possible at levels up to 500 micromol l(-1). In the presence of chlorophenols, normal root growth was possible only in concentrations not exceeding 50 micromol l(-1), except for I. batatas which was severely affected at all concentrations. Despite the reduction in biomass, the growth of S. aviculare cultures was sustained in medium containing up to 2000 micromol l(-1) of phenol or 2-chlorophenol, and up to 500 micromol l(-1) of 2,6-dichlorophenol. The amounts of phenol removed by the roots within 72 h of treatment were 72.7%, 90.7% and 98.6% of the initial concentration for D. carota, I. batatas and S. aviculare, respectively. For the removal of 2,6-dichlorophenol the values were, respectively, 83.0%, 57.7% and 73.1%. Phenols labelled with 14C were absorbed by the root tissues and condensed with highly polar cellular substances as well as being incorporated into the cell walls or membranes. The results suggest that S. aviculare, an ornamental plant, would be best suited for remediation trials under field conditions.     

GC and GC-MS characterization of crude oil transformation in sediments and microbial mat samples after the 1991 oil spill in the Saudi Arabian Gulf coast

The massive oil discharge in the Saudi Arabian coast at the end of the 1991 Gulf War is used here as a natural experiment to study the ability of microbial mats to transform oil residues after major spills. The degree of oil transformation has been evaluated from the analysis of the aliphatic and aromatic hydrocarbons by gas chromatography (GC) and GC coupled to mass spectrometry (GC-MS). The oil-polluted microbial mat samples from coastal environments exhibited an intermediate degree of transformation between that observed in superficial and deep sediments. Evaporation, photo-oxidation and water-washing seemed to lead to more effective and rapid elimination of hydrocarbons than cyanobacteria and its associated microorganisms. Furthermore, comparison of some compounds (e.g. regular isoprenoid hydrocarbons or alkylnaphthalenes) in the oil collected in the area after the spill or in the mixtures retained by cyanobacterial growth gave rise to an apparent effect of hydrocarbon preservation in the microbial mat ecosystems.     

Genetic toxicity in surface water from Guaíba Hydrographic Region under the influence of industrial, urban and agricultural sewage in the Drosophila wing-spot test

Mutagenic and recombinagenic activity of surface waters in the Guaíba Hydrographic Region (RS, Brazil) was investigated using the SMART in Drosophila melanogaster. Two positive results in Caí River (September 2000 and August 2001) and in Taquari River (August 2001 and February 2002)--linked to direct recombinagenic toxicants were observed. In Jacuí samples, an indirect mutagenic and recombinagenic action was detected in a September 2000 collection and a direct recombinational activity was observed in February 2002. Also in February 2002--samples from Dilúvio Brook and Guaíba Lake (GPC) were able to induce wing spots by mitotic recombinagenesis. The former sampling site showed toxicants to have a direct action, and the latter an increment in mitotic recombination that depended on metabolic action. The SMART wing test shows that all positive responses were mainly related to homologous mitotic recombination.     

Soil amendments reduce trace element solubility in a contaminated soil and allow regrowth of natural vegetation

We tested the effects of three amendments (a biosolid compost, a sugar beet lime, and a combination of leonardite plus sugar beet lime) on trace element stabilisation and spontaneous revegetation of a trace element contaminated soil. Soil properties were analysed before and after amendment application. Spontaneous vegetation growing on the experimental plot was studied by three surveys in terms of number of taxa colonising, percentage vegetation cover and plant biomass. Macronutrients and trace element concentrations of the five most frequent species were analysed. The results showed a positive effect of the amendments both on soil chemical properties and vegetation. All amendments increased soil pH and TOC content and reduced CaCl(2)-soluble-trace element concentrations. Colonisation by wild plants was enhanced in all amended treatments. The nutritional status of the five species studied was improved in some cases, while a general reduction in trace element concentrations of the aboveground parts was observed in all treated plots. The results obtained show that natural assisted remediation has potential for success on a field scale reducing trace element entry in the food chain.     

Arsenic in sediments from the southeastern Baltic Sea

Arsenic occurs as a persistent constituent in many of the chemical weapons dumped into the Baltic Sea; it can be used as an indicator of leakage and dispersal of released munitions to the marine environment. Total arsenic was analysed in sediment samples taken from the Lithuanian economic zone in the Baltic Sea, which included samples from the chemical munitions dumpsite in the Gotland Basin and national monitoring stations in the southeastern Baltic Sea. Arsenic concentrations in sediments ranged from 1.1 to 19.0 mg kg(-1), with an average of 3.4 mg kg(-1). Although there was evidence of slightly elevated arsenic content in sediments near the weapons dumpsite, arsenic concentrations were nevertheless quite low relative to other investigations in the Baltic and North Seas.     

On the determination of the laminar burning velocity from closed vessel gas explosions

A methodology to determine the laminar burning velocity from closed vessel gas explosions is explored. Unlike other methods which have been used to measure burning velocities from closed vessel explosions, this approach belongs to the category which does not involve observation of a rapidly moving flame front. Only the pressure–time curve is required as experimental input. To verify the methodology, initially quiescent methane–air mixtures were ignited in a 20-l explosion sphere and the equivalence ratio was varied from 0.67 to 1.36. The behavior of the pressure in the vessel was measured as a function of time and two integral balance models, namely, the thin-flame and the three-zone model, were fitted to determine the laminar burning velocity. Data on the laminar burning velocity as a function of equivalence ratio, pressure and temperature, measured by a variety of other methods have been collected from the literature to enable a comparison. Empirical correlations for the effect of pressure and temperature on the laminar burning velocity have been reviewed and two were selected to be used in conjunction with the thin-flame model. For the three-zone model, a set of coupled correlations has been derived to describe the effect of pressure and temperature on the laminar burning velocity and the laminar flame thickness. Our laminar burning velocities are seen to fall within the band of data from the period 1953–2003. A comparison with recent data from the period 1994–2003 shows that our results are 5–10% higher than the laminar burning velocities which are currently believed to be the correct ones for methane–air mixtures. Based on this observation it is concluded that the methodology described in this work should only be used under circumstances where more accurate methods can not be applied.     

Humanitarian governance and resilience building: Ethiopia in comparative perspective

Humanitarian governance is usually understood according to the classic, Dunantist paradigm that accords central importance to international humanitarian agencies. However, this is increasingly paralleled by ‘resilience humanitarianism’ that focuses, among other things, on including national actors in humanitarian governance. This article views humanitarian governance as emerging through interactions between authorities, implementing agencies and communities. It is based on interactive ethnography in five countries by Partners for Resilience (PfR). Using the Theory of Change (ToC) tool, it analyses the various interpretations and priorities of actors involved in humanitarian problems, solutions and programme governance. For example, PfR had a ‘software’ focus, aiming to unlock communities’ potential for resilience, whereas communities and authorities preferred to receive tangible ‘hardware’ support. The findings highlight the crucial role of local authorities in shaping humanitarian aid. This is especially pertinent in view of the international agenda to localise aid, which requires the understanding and support of national actors in order to responsibly protect the vulnerable.     

Is ionizing radiation effective in removing pharmaceuticals from wastewater?

Environmental Science and Pollution Research - Wastewater and effluent discharges are the main causes of receiving water body pollution and important challenges in water quality management. Among...     

Designing the next generation of climate adaptation research for development

Adaptation research has changed significantly in recent years as funders and researchers seek to encourage greater impact, ensure value for money and promote interdisciplinarity across the natural and social sciences. While these developments are inherently positive, they also bring fresh challenges. With this in mind, this paper presents an agenda for the next generation of climate adaptation research for development. The agenda is based on insights from a dialogue session held at the 2016 Adaptation Futures conference as well as drawing on the collective experience of the authors. We propose five key areas that need to be changed in order to meet the needs of future adaptation research, namely: increasing transparency and consultation in research design; encouraging innovation in the design and delivery of adaptation research programmes; demonstrating impact on the ground; addressing incentive structures; and promoting more effective brokering, knowledge management and learning. As new international funding initiatives start to take shape, we underscore the importance of learning from past experiences and scaling-up of successful innovations in research funding models.

     

Activated carbon produced from waste coffee grounds for an effective removal of bisphenol-A in aqueous medium

Environmental Science and Pollution Research - Bisphenol-A is widely used chemical in industry and unfortunately often detected in natural waters. Considered as an emerging pollutant, bisphenol-A...