Corynebacterium glutamicum as an indicator for environmental cobalt and silver stress–A proteome analysis

Cobalt and silver are toxic for cells, but mechanisms of this toxicity are largely unknown. Analysis of Corynebacterium glutamicum proteome from cells grown in control and cobalt or silver enriched media was performed by two dimensional gel electrophoresis (2DE) followed by mass spectrometry. Our results indicate that the cell adapted to cobalt stress by inducing five defense mechanisms: Scavenging of free radicals, promotion of the generation of energy, reparation of DNA, reparation and biogenesis of Fe-S cluster proteins and supporting and reparation of cell wall. In response to the detoxification of Ag⁺ many proteins were up-regulated, which involved reparation of damaged DNA, minimizing the toxic effect of reactive oxygen species (ROS) and energy generation. Overexpression of proteins involved in cell wall biosynthesis (1,4-alpha-glucan branching enzyme and nucleoside-diphosphate-sugar epimerase) upon cobalt stress and induction of proteins involved in energy metabolism (2-methylcitrate dehydratase and 1, 2-methylcitrate synthase) upon silver demonstrate the potential of these enzymes as biomarkers of sub-lethal Ag⁺ and Co toxicity.     

Characterization of distribution of pesticide residues in crop units

The characteristic features of distribution of pesticide residues in crop units and single sample increments were studied based on more than 19,000 residue concentrations measured in root vegetables, leafy vegetables, small-, medium- and large-size fruits representing 20 different crops and 46 pesticides. Log-normal, gamma and Weibull distributions were found to provide the best fit for the relative frequency distributions of individual residue data sets. The overall best fit was provided by lognormal distribution. The relative standard deviation of residues (CV) in various crops ranged from 15–170%. The 100–120 residue values being in one data set was too small to identify potential effects of various factors such as the chemical and physical properties of pesticides and the nature of crops. Therefore, the average of CV values, obtained from individual data sets, were calculated and considered to be the best estimate for the likely variability of unit crop residues for treated field (CV = 0.8) and market samples (CV = 1.1), respectively. The larger variation of residues in market samples was attributed to the potential mixing of lots and varying proportion of non-detects. The expectable average variability of residues in composited samples can be calculated from the typical values taking into account the sample size.     

快速断电安全技术中半导体中性点开关结构性能的研究

对我国目前半导体中性点开关的结构 ,工作过程 ,性能进行了分析 ;现行中性点开关所存在的问题为电路拓扑结构不合理 ,关断时间过长或过短 ,过长不满足快速断电安全技术的要求 ,过短又易引起过电压 ,使中性点开关失控。笔者提出了采用绝缘门极双极性晶体管 (IGBT) ,构成半导体中性点开关的可行方案。同时指出 ,开发新型快速断电安全技术装置 ,将大大提高我国煤矿安全技术水平     

基于多Agent技术的交通事故救援系统的研究

着重介绍了目前人工智能领域的研究热点问题———Agent技术的概念与原理 ,以及多Agent组的概念和分类 ;提出了基于多Agent技术的交通事故救援系统的流程框架 ;论述了该系统每一个Agent成员所能够完成的主要功能 ;通过该系统的实现 ,可以提高交通事故的及时报警与快速解决的效率 ,实行更加有效的交通流量合理分配 ;在利用既有交通基础设施的基础上增强了出行者的出行便利。笔者认为 ,随着Agent技术和网络技术研究的不断深入 ,基于Agent的应用研究必将有着更广泛的应用前景     

矿井主通风机叶轮磨损失效的评价

矿井主通风机叶轮严重磨损威胁着矿井安全生产 ,研究主通风机叶轮磨损和正确地评价叶轮的磨损失效 ,对煤矿安全生产具有重大的现实意义。应用气—固两相流动理论分析了主通风机叶轮的磨损部位 ,结论与现场叶轮的实际磨损相吻合。提出了叶轮磨损过程的 3个阶段。利用材料磨损理论分析了主通风机叶轮磨损机理 ,得出了叶轮磨损是以气体动力学磨料的冲蚀磨损为主 ,低应力擦伤型磨料磨损、腐蚀磨损为辅的结论。依据国家标准 ,结合矿井主通风机工作特点 ,从安全角度提出了矿井主通风机叶轮磨损永久失效的判据 ,并给出了判定标准。     

盾构施工对周围建筑物的安全影响及处理措施

讨论了盾构施工对周围建筑物的安全影响 ;分析了盾构通过建筑物时的组织方法 ;论述了盾构施工影响区域的划分和对建筑物影响进行预测的手段 ;提出了盾构通过建筑物时的施工控制参数和常见的工程处理措施。笔者就盾构施工引起的建筑物安全问题及其应对措施进行了论述和总结 ,为工程技术人员提供了有益的参考。     

桥梁实用延性抗震设计方法研究

针对铁路桥梁中应用广泛的单柱式桥墩 ,考虑多种跨度 ,多种墩高和多种场地及多种地震烈度的情况 ,在进行大量的桥墩结构线性和非线性弹塑性动力计算分析的基础上 ,通过对大量数据的统计分析 ,得到桥墩线弹性最大弯矩比和非线性位移延性比参数的变化规律 ,基于概率性的结构随机地震反应理论 ,建立了由线弹性动力分析结果来估算桥墩结构的位移延性响应的方法。     

计算流体力学(CFD)中的迭代法及其并行计算方法

应用计算流体力学 (CFD)方法分析事故原因已被广泛采用 ,笔者针对事故理论分析和流体计算过程中 ,运用CFD方法所存在计算量大的问题 ,分析和讨论了几种古典迭代法及其并行计算方法