Identification of barley genotypes with low grain Cd accumulation and its interaction with four microelements

The variation in grain cadmium (Cd) concentrations was evaluated among 600 barley genotypes grown in the same field condition to select low Cd accumulating genotypes. The results showed that there is considerable genotypic variation in grain Cd concentrations in barley grain samples, with the mean concentration of 0.16 mg kg(-1) DW and the variation of 0 (not detected) to 1.21 mg kg(-1) DW, and 47.2% of the grain samples exceeded the maximum permissible concentration (MPC) for Cd in cereal grains. In addition, differences between genotypes over the two years were fairly consistent, and Beitalys and Shang 98-128 showed the lowest grain Cd concentration, being 97.5% lower than that in the two highest Cd accumulators E-barley 6 and Zhenong 8 in the second harvest year. The great genotypic differences in Cd concentrations indicated that it is possible to lower Cd content of barley through cultivar selection and breeding for use at sites where Cd concentration in grain exceeds the MPC. Significant genotypic difference was also found in microelement concentrations. Correlation analysis showed that only Mn accumulation is synergetic with Cd accumulation, despite slightly positive relationship between Cd and Zn, Cu, or Fe in accumulation in barley grains.     

Excess copper induced physiological and proteomic changes in germinating rice seeds

Copper is an essential micronutrient for plants. Present at a high concentration in soil, copper is also regarded as a major toxicant to plant cells due to its potential inhibitory effects against many physiological and biochemical processes. The interference of germination-related proteins by heavy metals has not been well documented at the proteomic level. In the current study, physiological, biochemical and proteomic changes of germinating rice seeds were investigated under copper stress. Germination rate, shoot elongation, plant biomass, and water content were decreased, whereas accumulation of copper and TBARS content in seeds were increased significantly with increasing copper concentrations from 0.2mM to 1.5mM followed by germination. The SDS-PAGE showed the preliminary changes in the polypeptides patterns under copper stress. Protein profiles analyzed by two-dimensional electrophoresis (2-DE) revealed that 25 protein spots were differentially expressed in copper-treated samples. Among them, 18 protein spots were up-regulated and 7 protein spots were down-regulated. These differentially displayed proteins were identified by MALDI-TOF mass spectrometry. The up-regulation of some antioxidant and stress-related proteins such as glyoxalase I, peroxiredoxin, aldose reductase, and some regulatory proteins such as DnaK-type molecular chaperone, UlpI protease, and receptor-like kinase clearly indicated that excess copper generates oxidative stress that might be disruptive to other important metabolic processes. Moreover, down-regulation of key metabolic enzymes like alpha-amylase or enolase revealed that the inhibition of seed germinations after exposure to excess copper not only affects starvation in water uptake by seeds but also results in failure in the reserve mobilization processes. These results indicate a good correlation between the physiological and biochemical changes in germinating rice seeds exposed to excess copper.     

Removal of Prussian blue from contaminated soil in the rhizosphere of cyanogenic plants

The fate of radiolabeled cyanide in soil was investigated during exposure to cyanogenic plant species, sorghum (Sorghum bicolor var. P721) and flax (Linum usitassimum var. Omega-Gold), in fully-contained growth chambers. Labeled cyanide was subject to microbial transformation, assimilation by plant roots, incorporation and biodegradation in plant tissue. For this study, (14)C-labeled cyanide was added to soil, and distribution of (14)C activity was assessed before plant establishment and after harvest. After 3 months of plant growth, 7% of the (14)C-labeled cyanide was converted to (14)CO(2) with sorghum and 6% with flax, compared with only 2% conversion in unplanted soil. A small amount of unaltered cyanide was shown to be accumulated by the plants (approximately 140 mg cyanide/kg plant or <0.1% of the total). Results from this experiment demonstrate the potential of cyanogenic plants for use in phytoremediation of cyanide-contaminated soil.     

等离子体引发自由基增强光催化降解医用PVC的研究

研究了等离子体引发的自由基对锐钛矿TiO₂光催化降解医用聚氯乙烯（PVC）的影响.等离子体改性前后PVC的表面特性通过接触角、表面张力、X-光电子能谱（XPS）、电子自旋共振（ESR）表征.结果表明,等离子体改性后PVC的表面自由能和润湿性增强,水、丙三醇、硫代双乙醇的接触角减小.ESR表明等离子体改性后PVC表面的自由基增加了10倍.此外,通过重量损失和扫描电镜（SEM）对比了PVC-TiO₂和等离子体改性PVC-TiO₂的光催化降解,紫外光照60 h后,等离子体改性PVC-TiO₂的失重率比PVC-TiO₂增加了27.4%,SEM显示光照后等离子体改性PVC-TiO₂的表面有大量的裂纹.等离子体改性后PVC的光催化降解加剧.     

N1923络合萃取丙酸的研究

分别以模拟和实际丙酸发酵废水体系为研究对象,采用络合萃取法回收稀溶液中的丙酸,结果表明,对含2%丙酸的水溶液,以45%(体积比)的N1923为萃取剂,以煤油和正辛醇为稀释剂进行萃取,在油水相比1∶4的情况下,单级萃取率可达97%.对实际发酵废水中丙酸的萃取,在二级错流萃取的情况下,也能达到同样的分离效果.有机溶剂循环使用实验表明,经过10次重复利用后其萃取能力基本不变.     

Enhanced biohydrogen generation from organic wastewater containing NH 4+ by phototrophic bacteria Rhodobacter sphaeroides AR-3

NH₄⁺ is typically an inhibitor to hydrogen production from organic wastewater by photo-bacteria. In this experiment, biohydrogen generation with wild-type anoxygenic phototrophic bacterium Rhodobacter sphaeroideswas found to be sensitive to NH₄⁺ due to the significant inhibition of NH₄⁺ to its nitrogenase. In order to avoid the inhibition of NH₄⁺ to biohydrogen generation by R. sphaeroides, a glutamine auxotrophic mutant R. sphaeroides AR-3 was obtained by mutagenizing with ethyl methane sulfonate. The AR-3 mutant could generate biohydrogen efficiently in the hydrogen production medium with a higher NH₄⁺ concentration, because the inhibition of NH₄⁺ to nitrogenase of AR-3 was released. Under suitable conditions, AR-3 effectively produced biohydrogen from tofu wastewater, which normally contains 50–60 mg/L NH₄⁺, with an average generation rate of 14.2 mL/L·h. This generation rate was increased by more than 100% compared with that from wild-type R. sphaeroides.     

中国循环经济基本法立法架构研究

循环经济基本法立法架构的好与坏，不仅关系循环经济基本法的制定与出台，而且影响循环经济基本法的理解和执行。基于此。就循环经济基本法立法架构展开了综合研究，以期有助于中国循环经济基本法的制定与执行。在比较研究国外循环经济基本法立法架构与综合分析中国循环经济基本法立法架构研究现状的基础上，深入探讨中国循环经济的基本内涵以及中国循环经济基本法的基本定位与基本内容，进而提出一种新的中国循环经济基本法立法架构。从目前实践看，中国特色循环经济的内涵可以概括为是对生产和消费活动中物质能量流动方式的管理经济。中国循环经济建设应该从适度生产系统、适度消费系统和回收利用系统三个环节着手。根据经济基础决定上层建筑的基本原理，中国循环经济基本法作为一部环境友好型经济法以及具有一定强制性与制裁性的促进法，应当按照适度生产-适度消费-回收利用这条主线进行立法架构。     

国外"棕地再开发"土地利用策略及对我国的启示

棕地再开发是当代西方国家重要的城市可持续发展策略之一。本文界定了棕地的概念与特征，分析了其成因及棕地再开发的环境、社会、经济效益和广阔前景。并将棕地再开发与其他城市可持续发展战略如紧凑城市和理性增长，及城市用地改造策略如旧城更新和工业区置换进行了比较与区分，指出它在城市土地可持续发展与循环利用方面的优势。接着论述了棕地再开发策略在美国、英国及欧洲其他国家的实践情况，对其再利用模式和机制进行了归纳，这些国家制定与实施了不少环境与经济方面的宏观计划与实施细则，通过政策扶持、资金注入、税收减免、交通引导、保险保障、基建投资等途径大力支持和引导棕地再开发。最后明确了在我国实施棕地再开发策略的优势，认为要遵循“政府宏观调控、企业市场运作”的原则，推进土地循环使用，实现可持续发展和循环经济的战略目标。     

西北干旱半干旱区生态系统影响因子初探

通过分析百年尺度上中国西北降水空间分布规律和沙漠界线、农牧交错带界线的演变规律,认为:①干旱、半干旱是西北地区的主要气候特征;②生态系统非常敏感和脆弱;③在维持温饱和经济利益驱动下建立的农、牧业产业结构,是造成西北环境恶化的最主要的原因。