Presence of Torque Teno Virus (TTV) in Tap Water in Public Schools from Southern Brazil

Torque teno virus (TTV) was surveyed in tap water collected in schools from three municipalities located in the south of Brazil. TTV genomes were found in 11.7 % (4/34) of the samples. TTV DNA was detected in 10.5 % (2/19) of the samples collected at the city of Caxias do Sul and in 25 % (2/8) of the samples from Pelotas. Those cities have a low rate of sewage treatment. All samples from Santa Cruz do Sul, which has nearly 92 % of its sewage treated, were negative. These results suggest that the amount of sewage treated may have an effect on the detection rates of TTV DNA in drinking water in a given urban area, showing a mild negative correlation (r = ?0.76), when comparing the percentage of sewage treatment to the detection of TTV genomes. The detection rate of TTV was also compared with Escherichia coli, showing a strong correlation (r = 0.97), indicating that TTV may be a suitable marker of fecal contamination.     

Detection Method for Avian Influenza Viruses in Water

Recent events have shown that humans may become infected with some pathogenic avian influenza A viruses (AIV). Since soil and water, including lakes, rivers, and seashores, may be contaminated by AIV excreted by birds, effective methods are needed for monitoring water for emerging viruses. Combining water filtration with molecular methods such as PCR is a fast and effective way for detecting viruses. The objective of this study was to apply a convenient method for the detection of AIV in natural water samples. Distilled water and lake, river, and seawater were artificially contaminated with AIV (H5N3) and passed through a filter system. AIV was detected from filter membrane by real-time RT-PCR. The performance of Zetapor, SMWP, and Sartobind D5F membranes in recovering influenza viruses was first evaluated using contaminated distilled water. SWMP, which gave the highest virus recoveries, was then compared with a pre-filter combined GF/F filter membrane in a trial using natural water samples. In this study, the cellulose membrane SMWP was found to be practical for recovery of AIVs in water. Viral yields varied between 62.1 and 65.9% in distilled water and between 1 and 16.7% in natural water samples. The borosilicate glass membrane GF/F combined with pre-filter was also feasible in filtering natural water samples with viral yields from 1.98 to 7.33%. The methods described can be used for monitoring fresh and seawater samples for the presence of AIV and to determine the source of AIV transmission in an outbreak situation.     

Management strategies to mitigate the impacts of climate change on sea turtle’s terrestrial reproductive phase

Climate change poses a serious threat to sea turtles (Cheloniidae) as their terrestrial reproductive phase is only successful within a limited range of environmental and physical conditions. These conditions are likely to become less optimal as climate change progresses. To date, management and conservation of sea turtles has focused almost entirely on non-climatic stressors, due at least in part to practitioners not knowing what strategies to take and the feasibility and risks of potential strategies. To aid the management of sea turtles in a changing environment, we identified management strategies via a focus workshop and surveys to mitigate the impacts of climate change to the terrestrial reproductive phase of sea turtles. The effectiveness, ecological risks and potential social and logistical constraints associated with implementing each of the identified management strategies is discussed. Twenty management strategies were identified; strategies varied from habitat protection to more active and direct manipulation of nests and the nesting environment. Based on our results, we suggest a three-pronged approach to sea turtle conservation in light of climate change, where managers and researchers should: 1) enhance sea turtle resilience to climate change by mitigating other threats; 2) prioritise implementing the ‘no regret’ and ‘reversible’ management strategies identified here; and 3) fill the knowledge gaps identified to aid the trial and implementation of the potential strategies identified here. By combining these three approaches our collective toolkit of sea turtle management strategies will expand, giving us an array of viable approaches to implement as climate change impacts become more extreme.     

Design and manufacturing of femoral stems for the Indian population

Several researchers have stressed the need for a proper implant-patient match in hip joint replacements, in particular, for cementless femoral stems. Some of the complications of mismatch are aseptic loosening, improper load distribution, and discomfort. In this study, the differences in dimensions between femurs of elderly Indians and those of populations from other regions are compared in order to solve the problem of a possible geometric mismatch between a selected implant and the femurs of an Indian patients are concerned. This paper also describes a methodology for implant designing from measured anthropometric data, fit evaluation, finite element (FE) stress analysis and subsequently implant manufacturing using the CAM (Computer Aided Manufacturing) technique. The machining of the Titanium alloy femoral stem is usually performed on a 5 Axis CNC (Computer Numerical Control) machining center. However, in this study the machining of the femoral stem was effectively performed on a 3 Axis CNC machining center to emphasize on economical benefits for low cost manufacturing strategy.     

Incorporating stakeholder decision support needs into an integrated regional Earth system model

A new modeling effort exploring the opportunities, constraints, and interactions between mitigation and adaptation at regional scale is utilizing stakeholder engagement in an innovative approach to guide model development and demonstration, including uncertainty characterization, to effectively inform regional decision making. This project, the integrated Regional Earth System Model (iRESM), employs structured stakeholder interactions and literature reviews to identify the most relevant adaptation and mitigation alternatives and decision criteria for each regional application of the framework. The information is used to identify important model capabilities and to provide a focus for numerical experiments. This paper presents the stakeholder research results from the first iRESM pilot region. The pilot region includes the Great Lakes Basin in the Midwest portion of the United States as well as other contiguous states. This geographic area (14 states in total) permits cohesive modeling of hydrologic systems while also providing strong gradients in climate, demography, land cover/land use, and energy supply and demand. The results from the stakeholder research indicate that, for this region, iRESM should prioritize addressing adaptation alternatives in the water resources, urban infrastructure, and agriculture sectors, including water conservation, expanded water quality monitoring, altered reservoir releases, lowered water intakes, urban infrastructure upgrades, increased electric power reserves in urban areas, and land use management/crop selection changes. For mitigation in this region, the stakeholder research implies that iRESM should focus on policies affecting the penetration of renewable energy technologies, and the costs and effectiveness of energy efficiency, bioenergy production, wind energy, and carbon capture and sequestration.     

黄河入河沙漠颗粒物对磷酸盐的吸附特征

研究了黄河入河沙漠颗粒物在天然水(河水和海水)中对磷酸盐的吸附行为,用改进的Langmuir和Freundlich等温吸附模型对实验数据进行了拟合.结果表明,①改进的Langmuir等温吸附模型更适用于描述黄河入河沙漠颗粒物对磷酸盐的吸附特征,其物理意义更明确,得到的拟合参数更合理;②颗粒物对磷的最大吸附容量Qmax变化范围为41.322～357.143mg.kg-1,其中居延海颗粒物Y2的Qmax最大.Qmax与颗粒物(Y1～Y5)的有机质有着极显著的正相关关系;③除巴丹吉林沙漠颗粒物Y1外,颗粒物的临界磷平衡浓度EPC0值均大于其相应水体的磷浓度.Y1在黄河水中吸附磷的EPC0小于相应水体的磷浓度0.0100.053 mg.L-1,而在渤海海水中却大于相应水体的磷浓度0.1090.074 mg.L-1,表明Y1吸附了黄河水中的磷,进入渤海后Y1原有吸附可交换态磷NAP转移到海水中.其它颗粒物与相应水体进行磷交换的过程中存在着解吸磷的现象,具有向相应水体释放磷的能力;④黄河入河沙漠颗粒物对磷酸盐的吸附等温线是过溶液浓度轴0.00的交叉型等温线,这种新型交叉型吸附-解吸模型较好地解释了其吸附特征和天然粒子的双重性作用.     

Last Glacial mammals in South America: a new scenario from the Tarija Basin (Bolivia)

The chronology, sedimentary history, and paleoecology of the Tarija Basin (Bolivia), one of the richest Pleistocene mammalian sites in South America, are revised here based on a multidisciplinary study, including stratigraphy, sedimentology, geomorphology, paleontology, isotope geochemistry, and ¹⁴C geochronology. Previous studies have indicated a Middle Pleistocene age for this classic locality. We have been able to obtain a series of ¹⁴C dates encompassing all the fossil-bearing sequences previously studied in the Tarija Basin. The dated layers range in age from about 44,000 to 21,000 radiocarbon years before present (BP), indicating that the Tarija fauna is much younger than previously thought. Glacial advances correlated to marine isotopic stages (MIS) 4 and 2 (ca. 62 and 20 ka BP, respectively) are also documented at the base and at the very top of the Tarija–Padcaya succession, respectively, indicating that the Bolivian Altiplano was not dry but sustained an ice cap during the Last Glacial Maximum. The results of this multidisciplinary study enable us to redefine the chronological limits of the Tarija sequence and of its faunal assemblage and to shift this paleontological, paleoclimatological, and paleoecological framework to the time interval from MIS 4 to MIS 2.     

Rollover injury: effects of near- and far-seating position, belt use, and number of quarter rolls

PURPOSE: Vehicle and occupant responses in rollovers are complex since many factors influence both. This study analyzes the following factors: 1) belt use, 2) seated position with respect to the lead side in the rollover, 3) another front occupant in the crash, and 4) number of quarter rolls. The aim was to improve our understanding of rollover injury mechanisms. METHOD: Rollover accidents were analyzed using 1992-2004 NASS-CDS data. The sample included adult drivers and right-front passengers. All occupants were evaluated and then a subset of non-ejected occupants was analyzed. Using roll direction and seating position, the sample was divided into near- and far-seated occupants. Injury and fatality risks were determined by seatbelt use, occupancy, rollover direction, and number of quarter rolls. Risk was defined as the number of injured (e.g., MAIS 3+) divided by the number of exposed occupants (MAIS 0-6). Significance in differences was determined. A matched-pair analysis was used to determine the risk of serious injury for near- and far-seated occupants who were either belted or unbelted in the same crash. RESULTS: For all occupants, serious injury risks were highest for far-seated, unbelted occupants at 18.1% +/- 4.8%, followed by near-seated unbelted occupants at 12.0% +/- 3.5%. However, the difference was not statistically significant. Belted near- and far-seated occupants had a similar injury risk of 4.3% +/- 1.2% and 4.0% +/- 1.2%, respectively. For non-ejected occupants, serious injury risk was 9.5% +/- 3.2% for far-seated unbelted occupants and 4.9% +/- 2.1% for near-seated unbelted occupants, not a statistically significant difference. Serious injury risk was similar for belted near- and far-seated non-ejected occupants, at 3.6% +/- 1.1%. Seatbelts were 64.2%-77.9% effective in preventing serious injury for all occupants and 62.1%-26.5% for far- and near-seated, non-ejected occupants, respectively. Based on the matched pairs, seatbelts were less effective for near-seated (5.0%) compared to far-seated (2.8%) occupant MAIS 3+F risks. This was similar for non-ejected occupants. An unbelted near-seated occupant increased the risk for a belted far-seated occupant by 2.2 times, whereas an unbelted far-seated occupant increased the risk for a belted near-seated occupant by 10.2 times. For all occupants, the risk of serious injury increased with the number of quarter rolls, irrespective of seated position. For near-seated occupants, seatbelt effectiveness was higher in < or =1 roll than 1+ roll, at 72.3% compared to 28.3%. For far-seated occupants, seatbelt effectiveness was similar in < or =1 and 1+ roll samples at 78.3% and 76.8%, respectively. Near-seated occupants had the lowest serious injury risk when they were the sole occupant in the vehicle. This was also true for non-ejected occupants. However, far-seated occupants had a lower injury risk when another occupant was involved in the crash. CONCLUSIONS: The effect of carrying another occupant appears to reduce the risk of serious injury to far-seated occupants. However, near-seated occupants are better off being the sole occupant in the vehicle. Seatbelt effectiveness was lowest at 28.3% for non-ejected, near-seated occupants in 1+ rolls. This finding deserves further evaluation in an effort to improve seatbelt effectiveness in rollovers. For belted drivers alone in a rollover, fatality risks are 2.24 times higher for the far- versus near-seated position. Analysis of rollovers by quarter turns indicates that occupants are both far-side and near-side in rollovers. The extent to which this confounds the relationship between roll direction, seating position, and injury risk is unknown.     

Establishment of facultative sexuals

The existence of sex is one of the major unsolved problems in biology. We use computer simulations to model conditions in which sex may first become established. We develop an individual-based population model and show that a hypothetical facultative sex gene can fix, provided that the initial cost is low. It is demonstrated that the equilibrium fitness in the population increases with increasing population size and decreasing mutation rate. The probability of the establishment of the sex gene is found not to be directly related to the fitness difference between the asexual and sexual populations. This change in fitness on changing the parameters of the model is investigated.     

限氧EGSB反应器内颗粒污泥性能研究

研究限氧EGSB反应器内颗粒污泥的沉淀性能、产甲烷活性、形态、抗温度和负荷冲击等特性,以分析限氧EGSB反应器长期高效稳定运行的可行性.结果表明,限氧运行使得颗粒污泥的沉速降低,但仍能保持20.07～51.86 m/h的高沉速,保证了限氧EGSB反应器内约42 g/L的高污泥浓度;加入适量氧并没有对甲烷菌产生毒害作用,反而有所提高,提高幅度为11.94%.限氧EGSB反应器内颗粒污泥表面和内部微生物没有出现明显的分区分布,中高进水浓度时没有出现甲烷八叠球菌的明显优势;限氧EGSB反应器内颗粒污泥在经历温度和COD负荷双重冲击后,COD去除率明显降低,出水VFA明显增高,产气量明显降低,甚至出现不产气的情况.COD去除率的恢复很快,仅需20 d;出水VFA和产气也逐渐恢复,但有所滞后;微生物的恢复要慢些,扫描电镜结果表明,有些颗粒污泥表面的微生物细胞仍存在收缩现象.