国产十种合成洗衣粉诱发华西大蟾蜍(Bufo bufo andrewsi)蝌蚪红细胞微核的研究

研究了国产10种洗衣粉对华西大蟾蜍(Bufo bufo andrewsi)蝌蚪红细胞微核的诱变,结果表明“活力28超浓缩无泡加香”洗衣粉,“TM”及“SD”洗衣粉(1.2,6.0及12.0ppm)诱发蝌蚪微核率增高。在我们实验条件下,另外7种合成洗衣粉中的5种在12.0ppm浓度组也引起微核率升高。     

吉林省天然矿泉水资源基本特征与开发战略

本文在分析矿泉水形成的地质背景基础上,论述了全省碳酸矿泉水以游离Co₂、偏硅酸含量高,尤其矿化度高,并富含锶、锂、锌为基本特征;硅酸矿泉水以含锶的淡矿泉水为基本特征。全省矿泉水分布广泛、泉点集中、资源丰富、水质优良、类型齐全,具有广阔的开发前景。近期火山活动与活动断裂是矿泉水形成机制的两种类型。各火山群与活动断裂带是寻找矿泉水新产地的有望地段。     

Exclusion of chromosomal mosaicism in amniotic fluid cultures: Efficacy of in situ versus flask techniques

Accurate diagnosis of mosaicism in amniotic fluid cell cultures represents a major problem. If insufficient cells are analysed, true fetal mosaicism may go undetected. False-positive diagnosis is also possible since a second cell line may arise in vitro and not reflect the true fetal genetic constitution. These difficulties apply to both flask and in situ culture techniques, to varying degrees. The relative accuracy of flask versus in situ culture techniques in excluding mosaicism was determined by statistical analysis of experimental data from ten pairs of mixed male-female amniotic fluid specimens. The data support the idea that the majority of in situ colonies are independent of one another. The following conclusions are drawn: (1) analysis of a single metaphase from a number of different colonies enhances the confidence for excluding mosaicism; (2) analysis of more than one cell per colony offers little advantage; (3) exclusion of a given level of mosaicism requires analysis of fewer metaphases using the in situ method; (4) the confidence for excluding mosaicism is high with both in situ and flask techniques, using the provided guidelines; and (5) it is shown that the two-stage approach used by many laboratories is currently the most efficient way to exclude mosaicism.