Water pollution of Sabarmati River—a Harbinger to potential disaster

River Sabarmati is one of the biggest and major river of Gujarat that runs through two major cities of Gujarat, Gandhinagar and Ahmedabad and finally meets the Gulf of Khambhat (GoK) in the Arabian Sea. A study was conducted to evaluate the water quality of this river, as it could possibly be one of the major sources for filling up Kalpasar, the proposed man-made freshwater reservoir supposed to be the biggest one in the world. A total of nine sampling stations were established covering 163 km stretch of the river from upstream of Gandhinagar city to Vataman near Sabarmati estuary. Physicochemical (temprature, pH, salinity, chloride, total dissolved solids, turbidity, dissolved oxygen, biochemical oxygen demand, phenol, and petroleum hydrocarbons), biological (phytoplankton), and microbiological (total and selective bacterial count) analyses indicated that the river stretch from Ahmedabad-Vasana barriage to Vataman was highly polluted due to perennial waste discharges mainly from municipal drainage and industries. An implementation of sustainable management plan with proper treatment of both municipal and industrial effluents is essential to prevent further deterioration of the water quality of this river.     

Persistence of thiacloprid residues on brinjal (Solanum melongena L.)

Thiacloprid, a neonicotinoid insecticide with novel mode of action, is found to be effective against several lepidopteran as well as hemipteran pests. The present studies were carried out to observe the persistence pattern of thiacloprid on brinjal and to suggest suitable waiting period for the safety of consumers. Persistence of thiacloprid in brinjal was studied following three applications of thiacloprid (Alanto 240 SC) at 180 and 360 g a.i. ha^?1 at 7 days interval. Residues of thiacloprid in brinjal were estimated by high-performance liquid chromatography and were confirmed by liquid chromatography–mass spectrometry. The average initial deposits of thiacloprid were observed to be 0.48 and 1.05 mg?kg^?1 on the brinjal fruit following third application of thiacloprid at recommended and double the recommended dosages, respectively. Half-life periods for thiacloprid were found to be 0.47 and 0.50 days at single and double the application rates, respectively. Residues of thiacloprid declined below its limit of quantification of 0.05 mg?kg^?1 after 3 and 5 days, respectively, at recommended and double the recommended application rates. Therefore, the use of thiacloprid does not seem to pose any risk hazard and a waiting period of 1 day is suggested for safe consumption of brinjal fruits.     

Comparative study of the microstructural and magnetic properties of fly ashes obtained from different thermal power plants in West Bengal, India

This paper deals with the physical nature of the fly ashes obtained from two thermal power plants, situated in West Bengal, India. The fly ash samples are characterized by using comprehensive techniques with an emphasis on their ultrafine nature. The particle sizes of the samples are estimated using scanning electron microcopy (SEM) and found to lie within 0.18–5.90 μm. For morphology and compositional analysis, we also use SEM coupled with energy dispersive X-ray spectrometry. From X-ray study of the fly ashes the nature of conglomeration is seen to be crystalline, and the major components are mullite (Al₆Si₂O₁₃) and quartz (SiO₂). The magnetic measurement of the fly ash samples was carried out by SQUID magnetometer. ⁵⁷Fe Mössbauer spectra are obtained using a conventional constant-acceleration spectrometer with a ⁵⁷Co/Rh Mössbauer source. The hyperfine parameters obtained, in general, support the findings as made from XRD analysis and provide a quantitative measure of different iron ions present in the samples. The paper presents experimental data on the physical aspects of the fly ash samples of the thermal power plants which comprise coarse, fine, and ultrafine magnetic particulate materials and attempts to provide an exhaustive analysis.     

Thiamethoxam degradation by <Emphasis Type="Italic">Pseudomonas</Emphasis> and <Emphasis Type="Italic">Bacillus</Emphasis> strains isolated from agricultural soils

Twelve bacterial species were evaluated to know the degradation pattern of thiamethoxam in liquid medium. All the bacterial species could actively degrade phorate in a mineral salt medium containing phorate (50 μg ml^–1) as sole carbon source. As these species have ability to degrade, we used these for the degradation of thiamethoxam—a neonicoitinoids. Screening of 12 active phorate-metabolizing bacterial species resulted in selection of Bacillus aeromonas strain IMBL 4.1 and Pseudomonas putida strain IMBL 5.2 causing 45.28 and 38.23 % thiamethoxam (50 μg ml^?1) reduction, respectively, in 15 days as potential thiamethoxam degrading species. These two bacterial species grew optimally at 37 °C under shake culture conditions in MSMT medium raised with initial pH of 6.0–6.5 and use of these optimum cultural conditions resulted in improved thiamethoxam degradation by these bacterial species. These species caused maximum thiamethoxam degradation only in the presence of thiamethoxam as sole source of carbon and energy and the same was reduced in the presence of easily metabolize able carbon (C₀ and C₁) and nitrogen ((N₀, N₁ and N₂) sources. This could be attributed to involvement of repressible metabolic pathways, reactions of which are inhibited by the presence of easily available nutrients for growth. Besides above, qualitative analysis of thiamethoxam residues by gas liquid chromatography revealed complete metabolization of thiamethoxam without detectable accumulation of any known thiamethoxam metabolites.     

Estimation of β-cyfluthrin and imidacloprid in okra fruits and soil by chromatography techniques

Dissipation of β-cyfluthrin and imidacloprid in okra was studied following three applications of a combination formulation of Solomon 300 OD (β-cyfluthrin 9 % + imidacloprid 21 %) @ 60 and 120 g a.i. ha^?1 at 7 days interval. Residues of β-cyfluthrin and imidacloprid in okra were estimated by gas liquid chromatography (GLC) and high performance liquid chromatography (HPLC), respectively. Residues of β-cyfluthrin were confirmed by gas chromatograph–mass spectrometry (GC-MS) and that of imidacloprid by high performance thin layer chromatography (HPTLC). Half-life periods for β-cyfluthrin were found to be 0.91 and 0.68 days whereas for imidacloprid these values were observed to be 0.85 and 0.96 days at single and double the application rates, respectively. Residues of β-cyfluthrin dissipated below its limit of quantification (LOQ) of 0.01 mg kg^?1 after 3 and 5 days at single and double the application dosage, respectively. Similarly, residues of imidacloprid took 5 and 7 days to reach LOQ of 0.01 mg kg^?1, at single and double dosages respectively. Soil samples collected after 15 days of the last application did not show the presence of β-cyfluthrin and imidacloprid at their detection limit of 0.01 mg kg^?1.     

Integrated Bacillus sp. immobilized cell reactor and Synechocystis sp. algal reactor for the treatment of tannery wastewater

The wastewater discharged from leather industries lack biodegradability due to the presence of xenobiotic compounds. The primary clarification and aerobic treatment in Bacillus sp. immobilized Chemo Autotrophic Activated Carbon Oxidation (CAACO) reactor removed considerable amount of pollution parameters. The residual untreated organics in the wastewater was further treated in algal batch reactor inoculated with Synechocystis sp. Sodium nitrate, K₂HPO₄, MgSO₄.7H₂O, NH₄Cl, CaCl₂·2H₂O, FeCl₃ (anhydrous), and thiamine hydrochloride, rice husk based activated carbon (RHAC), immobilization of Bacillus sp. in mesoporous activated carbon, sand filter of dimensions diameter, 6 cm and height, 30 cm; and the CAACO reactor of dimensions diameter, 5.5 cm and height, 30 cm with total volume 720 ml, and working volume of 356 ml. In the present investigation, the CAACO treated tannery wastewater was applied to Synechocystis sp. inoculated algal batch reactor of hydraulic residence time 24 h. The BOD₅, COD, and TOC of treated wastewater from algal batch reactor were 20?±?7, 167?±?29, and 78?±?16 mg/l respectively. The integrated CAACO system and Algal batch reactor was operated for 30 days and they accomplished a cumulative removal of BOD₅,COD, TOC, VFA and sulphide as 98 %, 95 %, 93 %, 86 %, and 100 %, respectively. The biokinetic constants for the growth of algae in the batch reactor were specific growth rate, 0.095(day^?1) and yield coefficient, 3.15 mg of algal biomass/mg of COD destructed. The degradation of xenobiotic compounds in the algal batch reactor was confirmed through HPLC and FT-IR techniques. The integrated CAACO–Algal reactor system established a credible reduction in pollution parameters in the tannery wastewater. The removal mechanism is mainly due to co-metabolism between algae and bacterial species and the organics were completely metabolized rather than by adsorption.     

High reduction of 4-nitrophenol using reduced graphene oxide/Ag synthesized with tyrosine

There is a demand for the development of environmental friendly methods for the synthesis of graphene composites. Reduced graphene oxide/silver (RGO/Ag) nanocomposites are very good catalysts. Here, we propose a simple, green method for the synthesis of RGO/Ag nanocomposite using the amino acid tyrosine as bioreductant and stabilizing agent. RGO/Ag nanocomposite was characterized by using various analytical techniques and studied for its catalytic degradation of 4-nitrophenol. Results of attenuated total reflectance Fourier transform infrared spectroscopy and Zeta potential at ?55 mV reveal the surface capping of tyrosine onto the reduced graphene oxide nanosheets. RGO/Ag nanocomposites show excellent catalytic reduction of 4-nitrophenol with NaBH₄, when compared to actual individual silver nanoparticles.     

Volatilization of mercury from natural water by a broad-spectrum Hg-resistantBacillus pasteurii strain DR2

Summary A broad-spectrum mercury-resistant bacterial strain was isolated from contaminated water and was identified as Bacillus pasteurii strain DR2. It could volatilize Hg-compounds including organomercurials from its growth media. It utilized several aromatic compounds as a sole source of carbon. The bacterial strain eliminated HgCl₂ from sterile river water and the presence of benzene, toluene, naphthalene and nitrobenzene at 1 mM concentration in the system increased the rate of mercury volatilization, the volatilization rate being highest with benzene. When 1.7×10⁷ cells of this bacterial strain were added per ml of non-sterile water the bacterial strain volatilized more than 90 percent of mercury from mercuric chloride and organo-mercurials like PMA, thiomersol and methoxy ethyl mercuric chloride (MEMC). In the absence of this bacterial strain the volatilization of PMA and MEMC due to the presence of other Hg-resistant organisms in nonsterile polluted water ranged between 20–25 percent and of HgCl₂ was about 40 percent. However, in the presence of B. pasteurii DR2 volatilization of these Hg-compounds from non-sterile water increased by 20–40 percent. In the presence of 1 mM benzene the rate of mercury volatilization was even higher. In all the cases the rate of volatilization was higher in the first seven days than in the next seven days.Professor A. Mandal, MSc, PhD is Head of the Department of Biochemistry at the University College of Science, 35 Ballygunge Circular Road, Calcutta 700019, to whom correspondence should be addressed. His co-workers are Dr K. Pahan, Postdoctoral Associate, Department of Cell Biology and Paediatrics, Medical University of South Carolina, USA; Dr J. Chaudhuri, Senior Lecturer, Department of Molecular Biology, BKC College, Calcutta, India; Dr D.K. Ghosh, Postdoctoral Associate, Department of Biochemistry, University College of Science, Calcutta, India; Dr R. Gachhui, Postdoctoral Associate, The Cleveland Clinic Foundation, Cleveland, USA; and Dr S. Ray, Postdoctoral Associate, The Johns Hopkins University School of Hygiene and Public Health, Department of Biochemistry, Baltimore, USA.     

Greenhouse gas emissions from rice based cropping systems: Economic and technologic challenges and opportunities

Recent market slump in rice, less rainfall during monsoon, high temperature and scarcity of water during dry season leads to lower grain yield and less profit from rice cultivation in India. Farmers’ grow upland crops like chickpea (Cicer arietinum), greengram (Vigna radiate), mustard (Brassica nigra), corn (Zea maize), pigeonpea (Cajanus cajan), potato (Solanum tuberosum), sunflower (Helianthus annuus) etc. along with rice (Oryza sativa) during the dry season. However, knowledge of greenhouse gas (GHG) emission from these rice based cropping systems is very limited. In the present study four rice based cropping systems was studied along with rice-rice rotation system as control in respect of GHG emission, yield potential and economic feasibility. Conventional plantation and fertilizer application methodology was followed for each crop. Methane (CH₄) and nitrous oxide (N₂O) flux from field plots were studied with conventional closed chamber method using gas chromatograph. CH₄ flux was recorded highest from rice-rice rotation plots (304.25 kg ha⁻¹). N₂O flux was recorded 1.02 kg ha⁻¹ from rice-rice rotation system during wet season. However, during wet season, higher N₂O flux (1.93 kg ha⁻¹) was recorded from rice-potato-sesame rotation plots. Annual N₂O flux was also recorded significantly low (3.42 kg ha⁻¹) from rice-rice rotation plots and high (6.19 kg ha⁻¹) from rice-chickpea-greengram rotation plots. Significantly lower annual grain yield was recorded from rice-rice rotation plots (9.25 Mg ha⁻¹) whereas it was 18.84 Mg rice eq ha⁻¹ from rice-potato-sesame rotation system. The global warming potential (GWP) of rice-rice rotation system was recorded significantly high (8.62 Mg CO₂ ha⁻¹) compare to plots with different rice based cropping systems. Computing all C-emission from cradle-to-grave, highest total C-cost was recorded from the rice-rice rotation system ($62.00 ha⁻¹). We have made an attempt to calculate the C-credit of different rice based cropping systems by considering the difference of C-cost with control. The study suggests that the rice-potato-sesame is most sustainable among different cropping system studied in terms of economic profit ($62.00 ha⁻¹). We have made an attempt to calculate the C-credit of different rice based cropping systems by considering the difference of C-cost with control. The study suggests that the rice-potato-sesame is most sustainable among different cropping system studied in terms of economic profit (1248.21 ha⁻¹) and C-credit ($38.60 ha⁻¹). The result of the study may be limited to the study region; however, the study has potential use in respect to the development of agriculture practice for adaptation to climate change.     

A study on abundance and distribution of mangrove species in Indian Sundarban using remote sensing technique

Conservation and management of Sundarban mangrove forest is difficult chiefly due to inaccessibility and hostile condition. Remote sensing serves as an important tool to provide up-to date baseline information which is the primary requirement for the conservation planning of mangroves. In this study, supervised classification by maximum likelihood classifier (MLC) has been used to classify LANDSAT TM and LANDSAT ETM satellite data. This algorithm is used for computing likelihood of unknown measurement vector belonging to unknown classes based on Bayesian equation. Image spectra for various mangrove species were also generated from hyperspectral image. During field visits, GPS locations of five dominant mangrove species with appreciable distribution were taken and image spectra were generated for the same points from hyperion image. The result of this classification shows that, in 1999 total mangrove forest accounted for 55.01 % of the study area which has been reduced to 50.63 % in the year 2010. Avicennia sp. is found as most dominating species followed by Excoecaria sp. and Phoenix sp. but the aerial distribution of Avicennia sp., Bruguiera sp. and Ceriops sp. has reduced. In this classification technique the overall accuracy and Kappa value for 1999 and 2010 are 80 % and 0.77, 85.71 % and 0.81 respectively.