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131.
Ramos AS Gonçalves F Antunes SC Nunes B 《Journal of environmental science and health. Part. B》2012,47(6):512-519
This study characterizes the cholinesterasic forms present in the bivalve Corbicula fluminea, through the analysis of differential affinity towards various substrates and inhibitors. This approach also permitted the validation of cholinesterase inhibition in the mentioned species for monitoring purposes by evaluating the inhibitory activity of a detergent (SDS) and a pesticide (chlorfenvinphos), both in vitro and in vivo. The cholinesterasic form present in the tissues of C. fluminea showed intermediate properties in terms of its relation with substrates and inhibitors. Furthermore, it was not inhibited by the detergent SDS under any of the test conditions, but was clearly inhibited by the selected organophosphate. The data show clearly that the main cholinesterasic form in C. fluminea is responsive to the organophosphate chlorfenvinphos, thus supporting the use of this biomarker for the environmental monitoring of specific contaminants such as anticholinesterasic pesticides. Despite not being commonly attained in the wild, the levels of chlorfenvinphos required to elicit a significant ChE inhibition were only one order of magnitude above actual values of aquatic contamination by this pesticide. 相似文献
132.
Ana S. Ramos Fernando Gonçalves Sara C. Antunes Bruno Nunes 《Journal of environmental science and health. Part. B》2013,48(6):512-519
This study characterizes the cholinesterasic forms present in the bivalve Corbicula fluminea, through the analysis of differential affinity towards various substrates and inhibitors. This approach also permitted the validation of cholinesterase inhibition in the mentioned species for monitoring purposes by evaluating the inhibitory activity of a detergent (SDS) and a pesticide (chlorfenvinphos), both in vitro and in vivo. The cholinesterasic form present in the tissues of C. fluminea showed intermediate properties in terms of its relation with substrates and inhibitors. Furthermore, it was not inhibited by the detergent SDS under any of the test conditions, but was clearly inhibited by the selected organophosphate. The data show clearly that the main cholinesterasic form in C. fluminea is responsive to the organophosphate chlorfenvinphos, thus supporting the use of this biomarker for the environmental monitoring of specific contaminants such as anticholinesterasic pesticides. Despite not being commonly attained in the wild, the levels of chlorfenvinphos required to elicit a significant ChE inhibition were only one order of magnitude above actual values of aquatic contamination by this pesticide. 相似文献
133.
Sara E. McNamee Christopher T. Elliott Philippe Delahaut Katrina Campbell 《Environmental science and pollution research international》2013,20(10):6794-6807
A multiplex surface plasmon resonance (SPR) biosensor method for the detection of paralytic shellfish poisoning (PSP) toxins, okadaic acid (and analogues) and domoic acid was developed. This method was compared to enzyme-linked immunosorbent assay (ELISA) methods. Seawater samples (n?=?256) from around Europe were collected by the consortia of an EU project MIcroarrays for the Detection of Toxic Algae (MIDTAL) and evaluated using each method. A simple sample preparation procedure was developed which involved lysing and releasing the toxins from the algal cells with glass beads followed by centrifugation and filtering the extract before testing for marine biotoxins by both multi-SPR and ELISA. Method detection limits based on IC20 values for PSP, okadaic acid and domoic acid toxins were 0.82, 0.36 and 1.66 ng/ml, respectively, for the prototype multiplex SPR biosensor. Evaluation by SPR for seawater samples has shown that 47, 59 and 61 % of total seawater samples tested positive (result greater than the IC20) for PSP, okadaic acid (and analogues) and domoic acid toxins, respectively. Toxic samples were received mainly from Spain and Ireland. This work has demonstrated the potential of multiplex analysis for marine biotoxins in algal and seawater samples with results available for 24 samples within a 7 h period for three groups of key marine biotoxins. Multiplex immunological methods could therefore be used as early warning monitoring tools for a variety of marine biotoxins in seawater samples. 相似文献
134.
The dioxin/aryl hydrocarbon receptor (AhR) mediates most toxic effects of dioxins. In utero/lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) impairs fetal/neonatal development and the developing male reproductive tract are among the most sensitive tissues. TCDD causes antiestrogenic responses in rodent mammary gland and uterus and in human breast cancer cell lines in the presence of estrogen. Also, more recently an estrogen-like effect of TCDD/AhR has been suggested in the absence of estrogen. A transgenic mouse expressing a constitutively active AhR (CA-AhR) was developed as a model mimicking a situation of constant exposure to AhR agonists. Male and female reproductive tissues of CA-AhR mice were characterized for some of the effects commonly seen after dioxin exposure. Sexually mature CA-AhR female mice showed decreased uterus weight, while an uterotrophic assay in immature CA-AhR mice resulted in increased uterus weight. In immature mice, both TCDD-exposure and CA-AhR increased the expression of the estrogen receptor target gene Cathepsin D. When co-treated with 17β-estradiol no increase in Cathepsin D levels occurred in either TCDD-exposed or CA-AhR mice. In sexually mature male CA-AhR mice the weights of testis and ventral prostate were decreased and the epididymal sperm reserve was reduced. The results of the present study are in accordance with previous studies on dioxin-exposed rodents in that an activated AhR (here CA-AhR) leads to antiestrogenic effects in the presence of estrogen, but to estrogenic effects in the absence of estrogen. These results suggest the CA-AhR mouse model as a useful tool for studies of continuous low activity of the AhR from early development, resembling the human exposure situation. 相似文献
135.
This study employs insights largely derived from critical reflections on the common pool resources (CPR) theory to examine
the current governance arrangements in place to manage the mangrove forest at Kisakasaka, in Zanzibar, Tanzania. Kisakasaka
was used as a site for a community-based management pilot project of forest resources in Zanzibar. After some initial success
in setting up a local management structure and regulating access to the mangrove for mainly charcoal production, there are
now clear indications that forest conditions have deteriorated dramatically with concomitant ongoing resource use problems
for local villagers who have relied heavily on forest resources as a source of cash income. Extra-local factors, such as urban
population increases and associated market pressures for charcoal, are also conjectured to overlay and interact with the institutional
problems at Kisakasaka. As a result, over concern about the deterioration in the condition of the mangrove forest, the responsible
government authority decided not to renew the community-based governance arrangements after an initial five-year pilot period.
While revealing the inadequacies of existing governance arrangements and of its relationship to deteriorating forest conditions
at Kisakasaka, this study concludes by suggesting an approach to more fully understand forces driving local resource management
and use. 相似文献
136.
Sara Pantuliano 《Disasters》2007,31(S1):S77-S90
Despite more than 20 years of distribution of free emergency food in eastern Sudan (38 years in the case of refugees) Global Acute Malnutrition rates are currently the highest in the country. There has been no real improvement in the chronic livelihoods vulnerability that affects people in the region—particularly pastoralists. Food security must be seen from an informed livelihoods perspective. While food aid may still be required as part of transition, WFP must rethink its assistance strategy and advocate for complementarity in the efforts by development actors working across a range of sectors. Alternative models and interventions focused on the rehabilitation of markets and the development of' cargo nets' for the destitute, including cash transfers, must be developed and tested. Land tenure issues should be given particular attention. Food aid will probably remain an important element in the overall response, but its significance relative to other less developed forms of interventions should be reassessed. 相似文献
137.
In this paper, we explore how scenarios of future water withdrawals in a river basin are influenced by scale-dependent quantifications of the driving forces for two global-scale storylines. Either global-scale information or region-specific information is used to do the quantifications. In addition, we analyze the impact of including or not some restricted regional-scale information in the employed water use model. To develop scenarios of water withdrawals in the German part of the Elbe River basin, we applied the modules for domestic, thermoelectric power and manufacturing water use of the global water model WaterGAP, using scale-dependent driving forces scenarios and other scale-dependent model input. In the global-scale quantitative interpretations of the storylines of the IPCC SRES scenarios A1 and B2, all major driving forces of water withdrawals in the basin—population, thermoelectric power production and industrial gross domestic product—show vigorous increases between 2000 and 2025, while from the regional perspective, smaller increases but mostly decreases appear to be plausible. These discrepancies are partly due to the fact that for the global-scale interpretations only the historic developments until 1990 were taken into account, and not until 2000 as in the regional case. The resulting scenarios of sectoral water withdrawals in 2025 differ strongly between the two scale-dependent interpretations of the storylines, with the global one leading to much higher absolute water withdrawals and much lower withdrawal decreases between 2000 and 2025. Therefore, for regional assessments of water withdrawals, we recommend to embed the scenario analysis in global-scale storylines by performing regional-scale quantifications of the global qualitative driving forces scenarios, based on a limited amount of region-specific information. 相似文献
138.
Marco Vannini Elisha Mrabu Stefano Cannicci Rocco Rorandelli Sara Fratini 《Marine Biology》2008,153(6):1047-1053
In Mida Creek, Kenya (3°20′S, 40°5′E), at high water, the snail Cerithidea decollata dwells on the trunks of mangrove trees (Avicennia marina), while during low water it migrates to the ground, foraging at various distances from the trunk, where it aggregates again
well before the incoming tide. Snails from the upper shore level are 150–200 m distant from those living at the lower shore
level and they cluster at lower heights on trunks. In any case, sufficient height is usually attained to avoid being submersed.
An experiment was designed (February and October 2005), exchanging individuals from different shore levels subject to different
tide regimes, in order to test whether snails rely on internal information or on external, direct cues, to adapt their behaviour
to local conditions. Results show that C. decollata mostly rely on internal information, presumably based on an internal clock. When individuals from upper and lower shore levels
were exchanged, their internal clocks continued to govern when to ascend the home trunk and how high to climb for five to
six successive tides, after which the behaviour was reset to the new local conditions. 相似文献
139.
Although there is a large body of research on food webs in rocky intertidal communities, most of the emphasis has been on the marine benthic components. Effects of avian predation on highly mobile predators such as crabs, remains practically unstudied in rocky shore ecosystems. The crab, Cancer borealis, is an important component of the diet of gulls (Larus marinus, L. argentatus) at the Isles of Shoals, Maine, USA. C. borealis prey include the predatory gastropod Nucella lapillus L., the herbivore Littorina littorea, and mussels Mytilus edulis L. We hypothesized that gulls reduce abundance of C. borealis in the low intertidal and shallow subtidal, thereby allowing C. borealis prey to persist in high numbers. A study of crab tidal migration showed that C. borealis density nearly doubled at high tide compared to low tide; thus, crabs from a large subtidal source population migrate into the intertidal zone during high tides and either emigrate or are removed by gulls during low tides. Results from a small-scale (1 m2) predator caging experiment in the low intertidal zone indicated that enclosed crabs significantly reduced L. littorea abundance when protected from gull predation. In a much larger-scale gull exclusion experiment, densities of C. borealis increased significantly during low and high tides in exclosures relative to the controls. C. borealis density was inversely correlated with changes in the abundance of two mesopredators Carcinus maenas and Nucella lapillus, and with the space-occupier M. edulis. There was a similar negative correlation between abundance of C. borealis and the change in abundance of the herbivore L. littorea, but the trend was not significant. Mortality of tethered L. littorea was associated with C. borealis density across sites. However, preferred algae did not change in response to L. littorea density during the experiment. Thus, we found suggestive, but not conclusive, evidence for a three-level cascade involving gulls, crabs, and L. littorea. Our studies strongly suggest that gulls, as apex predators, generate three-level trophic cascades in rocky intertidal food webs by preventing the highly mobile subtidal predator, C. borealis, from establishing substantial populations in the low-mid intertidal zone thereby indirectly enhancing densities of two key mesopredators (N. lapillus, Carcinus) and blue mussels (M. edulis). 相似文献
140.
Ma Li Yu Zhuo Jiao Yan Lin Lin Zhong Wei Day Sara W. Postlethwaite Arnold Chen Hong Li Qiang Yin Heliang Wang Gang 《Environmental science and pollution research international》2021,28(28):37498-37505
Environmental Science and Pollution Research - The widespread epidemic of the COVID-19 in developed countries such as Europe and the USA has sparked many speculations. What factors caused the rapid... 相似文献