Monoclonal antibody produced by heterologous indirect enzyme-linked immunosorbent assay and its application for parathion residue determination in agricultural and environmental samples

A heterologous indirect enzyme-linked immunosorbent assay (ELISA) was developed, which was based on monoclonal antibody (Mab) to determine parathion residue in agricultural and environmental samples. Eight Mabs were produced by introducing the heterologous indirect ELISA into the screening procedure. It was shown that these Mabs had more broad-reactivity with twenty competitors than that of 5H7 (Mab produced by homologous screening). So it became much easier using these new Mabs to develop heterologous immunoassays. In addition, all the developed heterologous ELISAs could be used to determine parathion residue in semiquantitative or quantitative level, and their detection limitation (LOD) was around 2 ng/mL. Compared with the previous heterologous ELISA (hapten 11/5H7) with IC₅₀ of 13.3 ng/mL, a better heterologous ELISA (hapten 11/1E1) was obtained with IC₅₀ of 3.8 ng/mL, which improved the sensitivity 3.5 times. Finally, the latter was applied to parathion residue determination in spiked agricultural and environmental samples without extraction or cleanup. The average recoveries of parathion added to water, soil, cucumber, Chinese cabbage and carrot were between 80.4 % and 111.8 %. The LOD for water and soil samples was 5 ng/mL, and the LOD for cucumber, rice and corn samples was 10 ng/mL.     

Application of an enzyme-linked immunosorbent assay for the detection of clenbuterol residues in swine urine and feeds

The present study outlines applications of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol residues. Antisera were raised from rabbits immunized with diazotized clenbuterol-bovine serum albumin (BSA) conjugate. The assay was specific to clenbuterol with a half-maximum inhibition concentration (IC₅₀) of 1.8 ng/mL and 2.5 ng/mL in blank swine urine and phosphate buffer solution, respectively. The assay had high cross-reactivity (86%) with mabuterol, but low with other adrenergic agonists and antagonists. The average recovery of clenbuterol, as measured with the ELISA, ranged from 90% to 112% in swine urine samples and from 86% to 95% in feeds, respectively. This new assay was compared with commercial ELISA test kits. An excellent correlation (r ² = 0.98) between the two methods and satisfactory recoveries suggest that the new assay can be suitable for the determination of clenbuterol residues in real samples. The assay was used to analyze clenbuterol residues in 103 swine urine samples and 68 feed samples collected from northern China. Approximately 50% of the urine samples and 25% of the feed samples analyzed were found positive (concentration of clenbuterol ≥ 1 ppb). The results indicate that clenbuterol was misused in some of the areas surveyed.     

Medical waste management in China: A case study of Nanjing

Medical waste management is of great importance due to its infectious and hazardous nature that can cause undesirable effects on humans and the environment. The objective of this study was to analyze and evaluate the present status of medical waste management in the light of medical waste control regulations in Nanjing. A comprehensive inspection survey was conducted for 15 hospitals, 3 disposal companies and 200 patients. Field visits and a questionnaire survey method were implemented to collect information regarding different medical waste management aspects, including medical waste generation, segregation and collection, storage, training and education, transportation, disposal, and public awareness.The results indicated that the medical waste generation rate ranges from 0.5 to 0.8 kg/bed day with a weighted average of 0.68 kg/bed day. The segregated collection of various types of medical waste has been conducted in 73% of the hospitals, but 20% of the hospitals still use unqualified staff for medical waste collection, and 93.3% of the hospitals have temporary storage areas. Additionally, 93.3% of the hospitals have provided training for staff; however, only 20% of the hospitals have ongoing training and education. It was found that the centralized disposal system has been constructed based on incineration technology, and the disposal cost of medical waste is about 580 US$/ton. The results also suggested that there is not sufficient public understanding of medical waste management, and 77% of respondents think medical waste management is an important factor in selecting hospital services.The problematic areas of medical waste management in Nanjing are addressed by proposing some recommendations that will ensure that potential health and environmental risks of medical waste are minimized.     

基于协调发展视角的区域发展差异研究

20世纪90年代以来，区域差异研究成为学术界和政府管理者关注的热点领域。长期以来学者们主要以单一经济指标或复合经济指标作为衡量因子，分析区域经济差异时空演变及差异背后的自然、社会、经济驱动机理。从可持续发展的角度看，区域发展不单是区域经济的发展，而应是社会、经济与资源环境三方面的协调。基于这一理解，从可持续协调发展入手，建立了区域发展综合评价指标体系，选取了表征社会进步、经济发展和资源环境支持3个方面的27个因素作为评价指标，分析了区域综合发展的测算方法，提出了综合发展指数和发展协调指数。在案例分析中运用所设计的测度方法对江苏省内县级行政单位的综合发展进行了评价和分析。     

新疆哈密马庄山金矿区次火山岩及其地球化学特征

马庄山金矿区位于星星峡一卡瓦布拉克地块、古生代岛弧东段北侧活动大陆边缘，有多种火山宕、次火山岩和侵入岩，而与金矿成矿关系最为密切的是海西中、晚期次火山岩。该矿区次火山岩有花岗闪长斑岩、石英斑岩、花岗斑岩、隐爆角砾岩（简称花岗质岩）和辉绿岩。因此，在区域地质背景和矿区地质特征分析基础上，着重对次火山岩及其地球化学特征进行系统研究，结果认为．该矿区花岗质岩为高硅富钾贫铝钙碱性同熔（1）型花岗岩类，岩浆源区来自中下地壳；辉绿岩则为富钠碱性正常型基性岩类，岩浆源区来自下地壳。前者含Co、Pb、Zn、Sn、As、Bi、W、Mo、An、Ag、Tc等元素较高，有重要找矿前景；后者含Ba、Pb、Zn、Tl、As、Bi、Au、Ag、Tc等元素较高，也可为成矿提供物源。这些认识，对该区次火山岩特征、性质、成矿关系和构造背景，以及进一步找矿都有重要理论和实践指导意义。     

震后灾害损失快速评估

本文论述了震后灾害损失快速评估的三种方法,分别适用于城市、整个受灾区及农村。文中还提供了一系列平均统计数据,如建筑物震害率,平均损失率等,为当地无详细资料时参考使用。并以地震烈度和人口密度为参数,给出快速评估人员伤亡的经验公式。最后还讨论了地震间接损失的估算方法。     

Effects of rare earth elements La and Yb on the morphological and functional development of zebrafish embryos

In recent years, with the wide applications and mineral exploitation of rare earth elements, their potential environmental and health effects have caused increasing public concern. Effect of rare earth elements La and Yb on the morphological and functional development of zebrafish embryos were studied. The embryos were exposed to La³⁺ or Yb³⁺ at 0, 0.01, 0.1, 0.3, 0.5 and 1.0 mmol/L, respectively. Early life stage parameters such as egg and embryo mortality, gastrula development, tail detachment, eyes, somite formation, circulatory system, pigmentation, malformations, hatching rate, length of larvae and mortality were investigated. The results showed La³⁺ and Yb³⁺ delayed zebrafish embryo and larval development, decreased survival and hatching rates, and caused tail malformation in a concentration-dependent way. Moreover, heavy rare-earth ytterbium led to more severe acute toxicity of zebrafish embryo than light rare-earth lanthanum.     

餐厨垃圾综合资源化处理技术实例研究

在目前餐厨垃圾处理现状基础上,介绍了一种餐厨垃圾处理工艺,采用机械生物处理技术,包括收运系统、粉碎分选预处理、厌氧发酵生物处理、沼气热电联供、有机堆肥、废水处理、除臭系统多个环节,分离出油脂进行回收,垃圾厌氧发酵产生沼气发电,沼渣进行堆肥,以期实现餐厨垃圾减量化、无害化、资源化,解决"垃圾猪"、"地沟油"问题。     

共生细菌对盐生小球藻亚砷酸盐代谢的影响

为探讨共生细菌对盐生小球藻（Chlorella salina）亚砷酸盐（As（III））富集和形态转化的影响,从C.salina培养液中分离培养出一株共生细菌,并采用抗生素处理C.salina获得无菌藻,设置0,75,150,300,750 μg/LAs（III）溶液处理带菌和无菌的C.salina,7d后测定C.salina对As（III）的吸收、吸附和富集量,以及培养液和藻细胞内As的形态,计算带菌和无菌C.salina对As（III）的氧化率和去除率;使用不同浓度的As（III）处理共生细菌7d,并以300μg/L的As（III）处理共生细菌不同时间,计算两种情况下共生细菌对培养液中As（III）的氧化率和去除率.结果表明：C.salina的共生细菌为根癌农杆菌（Agrobacterium tumefaciens WB-1）;与无菌C.salina相比,带菌C.salina生长更快、对As（III）的耐性更强.带菌C.salina对As（III）的富集量为103.10~448.12mg/kg、氧化率为78.93%~96.88%、去除率为18.92%~55.21%,显著高于无菌C.salina的富集量（11.68~91.39mg/kg）、氧化率（14.46%~26.39%）和去除率（12.82%~29.15%）,也高于A.tumefaciens WB-1对As（III）的氧化率（4.51%~30.61%）和去除率（1.86%~16.19%）.此外,带菌C.salina胞内还检测到少量的As（III）和甲基砷,而在无菌C.salina胞内没有甲基砷存在.共生细菌促进了盐生小球藻对As（III）的富集和形态转化.