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541.
In the present study, we investigated to find novel ligands for low molecular weight environmental toxin, microcystin-LR (MC-LR) by using phage display technology. Two random libraries, displaying linear 12-mer peptides and cyclic 7-mer peptides, were screened against the immobilized target respectively. After three rounds of panning, phage clones that recognized microcystin-LR specifically were obtained from both the linear and the constrained libraries, proved by enzyme-linked immumosorbent assays and immunoprecipitation assays. DNA sequencing indicated that peptides displayed on some of the selected clones shared consensus sequences. Compared with traditional methods, this approach provided a cheaper and more rapid alternative to screen specific ligands for microcystin-LR. Moreover, since it is rather difficult to take small molecules as targets of phage display libraries, the success of this experiment expanded the applications of phage display technology, and provided a new avenue to study environmental small molecular toxins. 相似文献
542.
随着我国劳务派遣的快速发展,因劳务派遣而发生的侵犯被派遣劳动者合法权益的纠纷也层出不穷。与传统的劳动法律关系只有劳动者与用人单位两方主体不同,劳务派遣中涉及派遣单位、要派单位和被派遣劳动者三方的权利和义务。由于我国立法和理论研究的相对滞后,如何确认劳务派遣中的雇主以及雇主责任就成为各地司法机构和仲裁机构处理劳务派遣纠纷的焦点和难点。为此,笔者想谈谈自己的看法。 相似文献
543.
长江下游氮、磷含量变化及其输送量的估计 总被引:69,自引:10,他引:59
根据在1997和1998年内对长江干流大通站河水的3次采样分析,结合该站1962~1990年的水质资料,对长江下游N、P含量变化及其输送量进行研究.总氮、总磷的平均含量分别为1.6~2.2m g·L-1和0.11~0.15m g·L-1,N 以NO-3 -N 为主,约占76% ,其余主要为溶解有机氮和颗粒氮;P以颗粒态为主,占95% 以上.从5、6月到8月,NO-3-N含量是逐渐降低的,DON含量有较大幅度的上升,PN、PP及TP含量也有所增高.与历史资料相比,长江大通站溶解无机氮(DIN)和PO3-4-P含量还在不断上升,N、P含量的上升与流域化肥施用量变化趋势一致.1998年夏季洪水期间,约有79.95万tN和8.36万tP从大通站向下游输送,其中DIN与生物有效磷(BAP)比值远高于浮游植物生长的P限制值. 相似文献
544.
545.
交通运输是物质生产的一个特殊部门,是生产过程在消费领域中的再延续,它把生产与消费空间有机地连接起来,是实实在在的“流通领域”,它使人和物在全球范围内进行着生产、工作和学习的重新配置。交通活动是一个复杂的动态系统,在交通过程中人与人、人与车、人与路、车... 相似文献
546.
重大工业隐患的地域监控体系研究 总被引:6,自引:3,他引:3
沈立 《中国安全科学学报》2001,11(3):1-5
系统地分析了重大工业危险源与事故隐患 ;研究了地域监控、预警、应急预案的体系建设以及隐患监控的新技术应用基础 ;提出了职业安全卫生监控体系中的工业危险源和隐患监控。笔者认为 :应该分为过程安全监控和安全目标监控两个层次 相似文献
547.
548.
Yu-San Han Yu-Liang Sun Yi-Fen Liao I-Chiu Liao Kang-Ning Shen Wann-Nian Tzeng 《Marine Biology》2008,155(6):613-621
The Japanese eel has dramatically declined in Asia since the 1970s. Over the past two decades, glass eel productivity in Taiwan has decreased, but is highly variable among each year catch, though the cause for this is unknown. The impact of both population decline and instability on genetic diversity, however, is unknown. In this study, we investigated changes in allele frequencies of Japanese eel recruitment events over the past 20 years using six polymorphic microsatellite DNA loci. Specimens of glass eels were collected yearly from a single location in northern Taiwan from 1986 to 2007. Overall genetic differentiation among all samples was very low but significant (FST = 0.002, P = 0.002), and only 2 out of 120 pairwise tests were significant. The relationship between genetic and temporal distance showed a slight but insignificant correlation (R2 = 0.03, P = 0.0504). There were no overall significant differences in allelic richness (P = 0.35) or genetic heterozygosity (P = 0.73) among annual recruitment events. No apparent loss of genetic diversity and occurrence of a genetic bottleneck for eel populations were observed. Estimates of the effective population size (Ne) generally exceeded 500, although confidence intervals were very wide. While El Niño /Southern Oscillation (ENSO) events had little impact on genetic diversity, they may account for the annual fluctuation in glass eel catch. These results indicate long-term stability of genetic diversity in the Japanese eel with little evidence for sweepstakes recruitment. 相似文献
549.
The ecological toxicity of cadmium (Cd, 10 mg kg−1 of dry weight soil) and butachlor (10, 50 and100 mg kg−1 of dry weight soil) in both their single and combined effects on soil urease and phosphatase was studied after 1, 3, 7, 14,
21 and 28 days exposure under controlled conditions in paddy and phaeozem soils. The results showed that Cd reduced the activities
of urease and phosphatase at early incubation time (1–7 days), while the reduction almost disappeared at the end of the incubation.
The effect of Cd on phosphatase was more pronounced than that on urease. The activities of urease and phosphatase were reduced
by butachlor, while urease activity was significantly (P < 0.05 or P < 0.01) improved when the concentrations of butachlor were 10 and 50 mg kg−1 at the end of the incubation. When Cd (10 mg kg−1) was combined with butachlor (50 and 100 mg kg−1), the activities of urease and phosphatase became lower than without combination at early incubation time, which indicated
that the toxicity of Cd significantly increased (P < 0.05 or P < 0.01). However, when Cd (10 mg kg−1) was combined with butachlor (10 mg kg−1), the activities of urease and phosphatase became higher than those without combination at the end of the incubation, which
indicated that the toxicity of Cd decreased. It was indicated that the combined effects depended largely on the incubation
time and the concentration ratio of Cd and butachlor. In addition, it was showed that the combined effects of butachlor and
Cd appeared different in paddy from phaeozem, which may be related to the different properties of these soils. 相似文献
550.
进行了添加乙醇作为碳源强化油制气废水生物降解的研究,并利用GC/MS分析对油制气废水中芳烃类化合物的降解进行了初步研究。研究表明,共代谢基质乙醇的加入,可使菌种S-2、Y-3、XH-3、M-3对COD、氨氮、可萃取有机物等指标的去除率分别提高17.6%~25.6%、34.9%~42.8%、10.4%~14.2%;但在所采用的时间范围内,酚类化合物的去除率降低;芳烃类化合物的去除率提高15.4%~21.2%。除了维持无共代谢条件下对芳环数≤3的芳烃类化合物的良好降解能力外,对芳环数为4~6的化合物降解能力也有所提高。 相似文献