Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria, which cause lots of accidents and threatens human health. In this paper, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established and used to detect microcystin-LR (MC-LR) in drinking and surface waters. The concentration of coating antigen was 5 μ/mL, the dilution of monoclonal antibody MC10E7 was 1:3 000, the dilution of enzyme tracer (goat anti-mouse IgG-peroxidase) was 1:3 000, the standard concentration of MC-LR ranged from 0.001 μg/L to 30 μg/L, and o-phenylenediamine was used as substrate. The assay showed high relativity with high performance liquid chromatography (HPLC) with a correlation coefficient of more than 99%. The relative standard deviation was less than 10%, the detection limit was achieved down to 0.01 μg/L and up to 5.1 μg/L. The quantitative detection range was from 0.03 μg/L to 3 μg/L, and the antibody had high specificity for [4-arginine] microcystins. It performed well in spite of the influence of the real samples.     

Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria, which cause lots of accidents and threatens human health. In this paper, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established and used to detect microcystin-LR (MC-LR) in drinking and surface waters. The concentration of coating antigen was 5 μ/mL, the dilution of monoclonal antibody MC10E7 was 1:3 000, the dilution of enzyme tracer (goat anti-mouse IgG-peroxidase) was 1:3 000, the standard concentration of MC-LR ranged from 0.001 μg/L to 30 μg/L, and o-phenylenediamine was used as substrate. The assay showed high relativity with high performance liquid chromatography (HPLC) with a correlation coefficient of more than 99%. The relative standard deviation was less than 10%, the detection limit was achieved down to 0.01 μg/L and up to 5.1 μg/L. The quantitative detection range was from 0.03 μg/L to 3 μg/L, and the antibody had high specificity for [4-arginine] microcystins. It performed well in spite of the influence of the real samples. Translated from Environmental Science, 2006, 27(6): 1166–1170 [译自: 环境科学]     

石灰石湿法烟气脱硫添加剂的实验研究

利用双搅拌釜，作石灰石浆液脱硫的实验，测定添加剂强化前后石灰石浆液脱硫过程中气液两相传质系数的变化，对比气液两相传质系数的大小。并结合国内外脱硫技术现状，提出了以石灰石作为脱硫剂，以硫酸镁、硫酸钠、腐残酸钠为添加剂，应用双搅拌釜为主设备的湿法脱硫方法，对添加剂的脱硫机理进行了基础性研究。     

An improved method of direct chromosome preparation from chorionic villus and high resolution banding technique

The trophoblast was dissociated from the underlying mesenchymal layer either with acetic acid after short-term prefixation or with mechanical power after fixation twice. The colcemid treatment time was shorted to 16 min and trypsin solution of low pH (6.2) was used for banding. By these steps, the quality of chromosome banding was greatly improved and complete standard chromosome diagnoses were made in 24 of 24 cases. With the modified technique, high resolution banding chromosomes were consistently obtained after short-term incubation.     

克拉玛依炼油厂新污水处理场技术总结

介绍了污水处理工艺及流程，对装置运行情况进行了总结，分析存在的问题，并提出改进建议。     

环境灾害特点及其研究内容

对环境灾害的概念、特点、类型及主要研究内容进行了初步探讨。     

Interdisciplinary studies on the technical and economic feasibility of deep underground coal gasification with CO<Subscript>2</Subscript> storage in bulgaria

This paper presents the outcome of a feasibility study on underground coal gasification (UCG) combined with direct carbon dioxide (CO₂) capture and storage (CCS) at a selected site in Bulgaria with deep coal seams (>1,200 m). A series of state-of-the-art geological, geo-mechanical, hydrogeological and computational models supported by experimental tests and techno-economical assessments have been developed for the evaluation of UCG-CCS schemes. Research efforts have been focused on the development of site selection requirements for UCG-CCS, estimation of CO₂ storage volumes, review of the practical engineering requirements for developing a commercial UCG-CCS storage site, consideration of drilling and completion issues, and assessments of economic feasibility and environmental impacts of the scheme. In addition, the risks of subsidence and groundwater contamination have been assessed in order to pave the way for a full-scale trial and commercial applications. The current research confirms that cleaner and cheaper energy with reduced emissions can be achieved and the economics are competitive in the future European energy market. However the current research has established that rigorous design and monitor schemes are essential for productivity and safety and the minimisation of the potential environmental impacts. A platform has been established serving to inform policy-makers and aiding strategies devised to alleviate local and global impacts on climate change, while ensuring that energy resources are optimally harnessed.     

东湖通道工程施工废水的理化特性与处置对策研究

为认识东湖通道施工废水的基本特性,以不受施工干扰的湖心水样为对照,对东湖通道施工过程中产生的生产、生活废水进行了分析.结果发现,通道施工废水的主要特征是悬浮颗粒物、营养物质、还原性物质等物质的含量高,水体呈碱性;其中,坑道废水悬浮颗粒物多、浊度高;桩机打桩废水悬浮颗粒物多、浊度高、营养物质含量高;搅拌施工废水氧化还原电位高、悬浮颗粒物含量高;材料堆放废水高锰酸盐指数高,生活污水浊度高、营养物质和水中油的含量高,粪大肠杆菌群超标.坑道废水、桩机打桩废水、搅拌施工废水和生活污水均为劣Ⅴ类,材料堆放废水符合Ⅳ类水标准.针对东湖通道施工废水的理化特性,鉴于国家5A级景区对水质的较高要求,建议在施工区构建临时沉淀池、小型氧化塘等设施开展原位污废水处置,确保达标排放,避免影响水生态环境.     

Antibiotic exposure and potential risk of depression in the Chinese elderly: a biomonitoring-based population study

Environmental Science and Pollution Research - To examine the associations between urinary antibiotics from various sources and depression in the elderly using the biomonitoring method. In the...     

人与自然和谐是构建和谐社会的物质基础

在剖析和谐社会及人与自然和谐基本内涵的基础上。通过分析资源短缺、生态破坏和环境污染对社会和谐的破坏作用。阐明了人与自然和谐是构建和谐社会的基本前提；通过分析加强环境保护、合理开发利用资源可以促进民主法治和公平正义。促使社会充满活力、安定有序。促进经济繁荣和持续发展。阐明了以人与自然和谐推进和谐社会的构建。进而全面论述了人与自然和谐是构建和谐社会的物质基础。