Characterization of ACC deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of Brassica napus

One hundred Cu-resistant-endophytic bacteria were isolated from Cu-tolerant plants grown on Cu mine wasteland, of which, eight Cu-resistant and 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing endophytic bacteria were obtained based on the ACC deaminase activity of the bacteria and characterized with respect to metal resistance, production of ACC deaminase, indole-3-acetic acid (IAA) as well as siderophores and mineral phosphate solubilization. Ralstonia sp. J1-22-2, Pantoea agglomerans Jp3-3, and Pseudomonas thivervalensis Y1-3-9 with higher ACC deaminase activity (ranging from 213 to 370 μM α-ketobutyrate mg(-1)h(-1)) were evaluated for promoting plant growth and Cu uptake of rape grown in quartz sand containing 0, 2.5, and 5 mg kg(-1) of Cu in pot experiments. The eight bacteria were found to exhibit different multiple heavy metal resistance characteristics, to show different levels of ACC deaminase activity and to produce indole acetic acid. Seven bacteria produced siderophores and solubilized inorganic phosphate. Pot experiments showed that inoculation with the strains (J1-22-2, Jp3-3, and Y1-3-9) was found to increase the biomass of rape. Increases in above-ground tissue Cu contents of rape cultivated in 2.5 and 5 mg kg(-1) of Cu-contaminated substrates varied from 9% to 31% and from 3 to 4-fold respectively in inoculated-rape plants compared to the uninoculated control. The maximum Cu uptake of rape was observed after inoculation with P. agglomerans Jp3-3. The results show that metal-resistant and plant growth promoting endophytic bacteria play an important role in plant growth and Cu uptake which may provide a new endophytic bacterial-assisted phytoremediation of Cu-contaminated environment.     

中原油田石油污染土壤原位生物修复技术实验研究

通过实验室选择性富集培养,从中原油田石油污染土壤中获得了能以中原原油为碳源快速生长的石油降解菌群。结合黑麦草（Ryegrass）和苜蓿（Alfalfa）,采用该降解菌群对原油污染土壤进行了原位生物联合修复实验。接入降解菌的实验区分种植黑麦草、种植苜蓿、未种植区,另设黑麦草区和空白区。经过99 d的生物修复,石油烃累计降...     

Biodegradation of nicotine by a novel Strain Shinella sp. HZN1 isolated from activated sludge

The nicotine-degrading bacterium HZN1 was isolated from activated sludge and identified as Shinella sp. based on its physiological characteristics and analysis of 16S rDNA gene. Strain HZN1 is capable of using nicotine as the sole carbon source in the mineral salts medium. The optimum temperature and pH for strain HZN1 growth and nicotine degradation were 30°C and 7.0, respectively. It could degrade approximately 100 % of 0.5 g L(-1) of nicotine within 9 h. Three intermediate metabolites were produced by the strain HZN1 and identified as cotinine, myosmine and nicotyrine using gas chromatography-mass spectrometry. This is the first report of nicotine-degrading strain from the genus of Shinella. The results showed that strain HZN1 could be potentially employed in bioremediation of nicotine. Our findings would provide a new insight into the biodegradation of nicotine.     

Polybrominated diphenyl ethers (PBDEs) and indicator polychlorinated biphenyls (PCBs) in marine fish from four areas of China

The levels of polybrominated diphenyl ethers (PBDEs) and indicator polychlorinated biphenyls (PCBs) were determined in marine fish from four areas of China (South China Sea, Bohai Sea, East China Sea, and Yellow Sea) using GC/NCI-MS and GC/ITMS, respectively. Total concentrations of eight PBDEs (BDE-28, 47, 99, 100, 153, 154, 183 and 209) in all samples ranged from 0.3 ng g⁻¹ ww (wet weight) to 700 ng g⁻¹ ww, with median and mean values of 85 ng g⁻¹ ww and 200 ng g⁻¹ ww, respectively. BDE-209 and BDE-47 were the major congeners in all samples, contributing 54% and 19% to the total concentration, respectively. The sum of seven indicator PCB levels (CB-28, 52, 101, 118, 138, 153, and 180) ranged from 0.3 ng g⁻¹ ww to 3.1 μg g⁻¹ ww, with median and mean values of 6.4 ng g⁻¹ ww and 398 ng g⁻¹ ww, respectively. High contributions of CB-138 (32%) and CB-153 (25%) were found in all samples. In general, pollutants measured in this study were at high levels when compared with previous studies from other regions in the world. The relative abundance of BDE-209 may suggest that deca-BDE sources existed in studied area. And principal component analysis (PCA) showed that there were other PBDE sources in Yellow Sea. The pattern and PCA showed that PCB pollutions came from similar sources in the studied areas. In addition, concentrations of ∑₇PBDEs (u/209) were strongly correlated with those of ∑₇PCBs in all fish (r = 0.907, n = 44).     

活性炭涂层电极电容去离子的性能

采用活性炭涂层电极构建电容去离子吸附装置,以氯化钠模拟含盐原水,研究电压、流量、进水浓度等操作参数对活性炭涂层电极脱盐效率和能耗的影响。实验结果表明,去除率和比吸附量随着电压的增大而增加,且比能耗随之增大。流速越小,出水的浓度越低,当对出水的浓度要求较高时,宜采用小流速。当进水浓度低于活性炭涂层电极的饱和吸附量时,比吸附量随着进水浓度呈线性增加;当达到饱和吸附量时,比吸附量不会随进水浓度的增大而发生改变;比能耗随着进水浓度的增加而降低。     

长白山火山泥石流分布影响因子权重分析

利用层次分析法对长白山天池火山泥石流分布影响因子的权重进行了分析。将对长白山天池火山泥石流分布影响作为评价目标,将地质条件、地形地貌以及植被覆盖情况作为第一层次的评价因子,地层年代、地层岩性、风化程度、堆积厚度、高程、相对高差、纵坡比、植被类型、覆盖程度作为第二层次的评价因子,由评价目标以及2个层次的评价因子构成了层次结构模型。通过层次结构模型建立了2个层次的4个判断矩阵,通过对判断矩阵的计算,最终得到了各因子对长白山火山泥石流分布影响的权重值。其中地形地貌中的相对高差及纵坡比对长白山火山泥石流分布的影响最大。     

China's Regional Policies:Progress of the 11~(th) Five Year Plan Period and Prospect of the 12~(th) Five Year Plan Period

During the 11th Five Year Plan period,China improved its policy system for the overall strategy of regional development,and established and completed the policies intended for the promotion of regional cooperation and interaction.Thereupon,regional development in China took on a series of positive changes such as the enhancement of coordination.During the 12th Five Year Plan period,China will fully implement and continuously improve the policy measures for development in its four major regions,strengthen differentiated guidance to different regions in line with the planning for major function-oriented zones,and accelerate the establishment of basic systems that facilitate coordinated regional development.     

四川省自贡市蛋鸡场鸡粪处理工艺

本文主要阐述自贡市蛋鸡场在养鸡生产实践中面临着鸡粪处理这一难题，选用四川省农机所设计，并由四川省资中通风机械厂试剂的GZS-0.5型鸡粪干燥成套设备进行鸡粪加工的工作，本场对GZS-0.5型鸡粪干燥成套设备废气的净化处理有所改进创新。     

浙江东阳市环境污染现状,趋势及其对策

随着东阳市经济的迅速发展．由于人口数量增加，对自然资源的需求量越来越大，环境保护意识薄弱等原因，导致生态系统遭到破坏，林相单一、水土流失严重、洪旱灾害频繁、环境污染严重等灾害。据此，本文提出保护东阳市环境的对策。     

超补偿理论及其在虫害控制中的意义

本文较为系统地阐述了作物对虫害损伤的超补偿理论,表明超补偿广泛存在于各种作物中,并对超补偿所需条件、基本原因以及与害虫控制的关系作进一步讨论。