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961.
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Evaluation of the infectivity, gene and antigenicity persistence of rotaviruses by free chlorine disinfection 总被引:1,自引:0,他引:1
The effects of free chlorine disinfection of tap water and wastewater effluents on the infectivity, gene integrity and surface antigens of rotaviruses were evaluated by a bench-scale chlorine disinfection experiments. Plaque assays, integrated cell culture-quantitative RT-PCR (ICC-RT-qPCR), RT-qPCR, and enzyme-linked immunosorbent assays (ELISA), respectively, were used to assess the influence of the disinfectant on virus infectivity as well as genetic and antigenic integrity of simian rotavirus SA11 as a surrogate for human rotaviruses. The ICC-RT-qPCR was able to detect rotaviruses survival from chlorine disinfection at chlorine dose up to 20 mg/L (60 min contact), which suggested a required chlorine dose of 5 folds (from 1 to 5 mg/L) higher than that indicated by the plaque assay to achieve 1.8 log10 reductions in tap water with 60 min exposing. The VP7 gene was more resistant than the infectivity and existed at chlorine dose up to 20 mg/L (60 min contact), while the antigencity was undetectable with chlorine dose more than 5 mg/L (60 min contact). The water quality also impacted the inactivation efficiencies, and rotaviruses have a relatively higher resistant in secondary effluents than in the tap water under the same chlorine disinfection treatments. This study indicated that rotaviruses have a higher infectivity, gene and antigencity resistance to chlorine than that previously indicated by plaque assay only, which seemed to underestimate the resistance of rotaviruses to chlorine and the risk of rotaviruses in environments. Present results also suggested that re-evaluation of resistance of other waterborne viruses after disinfections by more sensitive infectivity detection method (such as ICC-RT-qPCR) may be necessary, to determine the adequate disinfectant doses required for the inactivation of waterborne viruses. 相似文献
963.
Seedling growth and metal accumulation of selected woody species in
copper and lead/zinc mine tailings 总被引:1,自引:0,他引:1
Xiang Shi Xiaolei Zhang Guangcai Chen Yitai Chen Ling Wang Xiaoquan Shan 《环境科学学报(英文版)》2011,23(2):266-274
A greenhouse pot experiment was conducted to evaluate the potential of selected woody plants for revegetation in copper (Cu)
and lead/zinc (Pb/Zn) mine tailing areas. Five woody species (Amorpha fruticosa Linn, Vitex trifolia Linn: var. simplicifolia Cham,
Glochidion puberum (Linn.) Hutch, Broussonetia papyrifera, and Styrax tonkinensis) and one herbaceous species (Sesbania cannabina
Pers) were planted in Cu and Pb/Zn tailings to assess their growth, root morphology, nutrition uptake, metal accumulation, and
translocation in plants. Amorpha fruticosa maintained normal growth, while the other species demonstrated stress related growth and
root development. Sesbania cannabina showed the highest biomass among the plants, although it decreased by 30% in Cu tailings and
40% in Pb/Zn tailings. Calculated tolerance index (TI) values suggested that A. fruticosa, an N-fixing shrub, was the most tolerant
species to both tailings (TI values 0.92–1.01), while S. cannabina had a moderate TI of 0.65–0.81 and B. papyrifera was the most
sensitive species, especially to Pb/Zn tailings (TI values 0.15–0.19). Despite the high concentrations of heavy metals in the mine
tailings and plants roots, only a small transfer of these elements to the aboveground parts of the woody plants was evident from the
low translocation factor (TF) values. Among the woody plants, V. trifolia var. simplicifolia had the highest TF values for Zn (1.32),
Cu (0.78), and Pb/Zn (0.78). The results suggested that A. fruticosa and S. cannabina, which have the highest tolerance and biomass
production, respectively, demonstrated the potential for tailings revegetation in southern China. 相似文献
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967.
结合中国石化HSE管理系统的建设情况,介绍了中国石化HSE管理系统建设的背景、系统总体架构、试点建设范围、系统功能及在试点企业的应用成效。 相似文献
968.
介绍了目前国内实施GHS的现状及面临的问题,通过分析《欧盟物质和混合物的分类、标签和包装法规》(CLP法规)的特点及其与GHS的异同,为我国实施GHS提出建议。 相似文献
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970.