Co-metabolic transformation of the neonicotinoid insecticide imidacloprid by the new soil isolate Pseudoxanthomonas indica CGMCC 6648

A new imidacloprid (IMI) degrading bacterium Z-9 (deposited number CGMCC 6648) was isolated and identified as Pseudoxanthomonas indica by 16S rRNA gene analysis. Two metabolites were identified as olefin and 5-hydroxy IMI by liquid chromatography-mass spectrometry and nuclear magnetic resonance analysis. P. indica CGMCC 6648 degraded 70.1% of IMI (1.22 mmol L^?1) and formed 0.93 mmol L^?1 5-hydroxy IMI and 0.05 mmol L^?1 olefin IMI in 6 days and in the presence of 100 mmol L^?1 glucose. The half-life of IMI degradation was 3.6 days. P. indica CGMCC 6648 transforms IMI via a co-metabolism mechanism and different carbohydrates have significant effects on 5-hydroxy IMI formation, whereas different organic acids have substantial effects on olefin IMI production. Lactose is the best co-substrate for IMI degradation and 5-hydroxy IMI formation with 0.77 mmol L^?1 degraded and 0.67 mmol L^?1 formed in 48 h, respectively. Pyruvate is the best co-substrate for olefin IMI formation with 0.17 mmol L^?1 produced in 96 h for all carbon sources tested. Pyruvate significantly stimulates the conversion of 5-hydroxy IMI to olefin IMI, whereas glucose slightly inhibits this reaction. P. indica CGMCC 6648 rapidly degrades IMI and forms olefin IMI, which may enhance its potential for biodegradation of IMI and increase its insecticidal activity, which can decrease the IMI dosage required.     

Medical waste management in China: A case study of Nanjing

Medical waste management is of great importance due to its infectious and hazardous nature that can cause undesirable effects on humans and the environment. The objective of this study was to analyze and evaluate the present status of medical waste management in the light of medical waste control regulations in Nanjing. A comprehensive inspection survey was conducted for 15 hospitals, 3 disposal companies and 200 patients. Field visits and a questionnaire survey method were implemented to collect information regarding different medical waste management aspects, including medical waste generation, segregation and collection, storage, training and education, transportation, disposal, and public awareness.The results indicated that the medical waste generation rate ranges from 0.5 to 0.8 kg/bed day with a weighted average of 0.68 kg/bed day. The segregated collection of various types of medical waste has been conducted in 73% of the hospitals, but 20% of the hospitals still use unqualified staff for medical waste collection, and 93.3% of the hospitals have temporary storage areas. Additionally, 93.3% of the hospitals have provided training for staff; however, only 20% of the hospitals have ongoing training and education. It was found that the centralized disposal system has been constructed based on incineration technology, and the disposal cost of medical waste is about 580 US$/ton. The results also suggested that there is not sufficient public understanding of medical waste management, and 77% of respondents think medical waste management is an important factor in selecting hospital services.The problematic areas of medical waste management in Nanjing are addressed by proposing some recommendations that will ensure that potential health and environmental risks of medical waste are minimized.     

火焰原子吸收光度法测定水中铝的方法改进

研究了在盐酸介质中，壬基酚聚氧乙烯-7醚（NP-7）活化下，火焰原子吸收光度法测定环境水体中铝的方法改进。在25mL容量瓶中，加入5．0mL体积分数为50％的盐酸、2．0mL质量浓度为0．01g／mL NP-7和4．0mL质量浓度为75．0μg/mL的铝标准溶液，在原子吸收分光光度计的最佳测定条件下测定吸光度。根据吸光度与铝质量浓度绘制了工作曲线，线性范围3．0～24．0μg/mL，检出限1．32μg/mL。该法用于环境水体中铝含量的测定，加标回收率为94．4％～101．4％，最大相对标准偏差5．8％，方法对比最大相对误差4．1％。     

利用市场机制的作用实现劳动安全监察任务

提出利用市场机制的作用，实现劳动安全监察任务的观点。简析计划经济体制下实现劳动安全监察任务的模式；利用市场机制的功能和特性，分析判别计划经济体制下形成的实现劳动安全监察任务途径及其手段、有效性和必然性；提出了具体进行工作的思路、方法。     

滑行车类游乐设施动力学建模与仿真

根据滑行车类游乐设施运行速度高、加速度大、载荷工况复杂,发生事故后果非常严重的现状,认为深入的动力学分析、结构强度校核和寿命分析预测对保证其安全性尤为必要。因而分析滑行车的结构原理,提出其多体系统拓扑构型,包括轮轨关系模型、各零部件之间约束模型。运用图形学方法研究了滑行车轨道建模参数提取和坐标变换技术。通过仿真分析,在设计阶段就可获得滑行车在不同工况下运行时各部件的速度、加速度及载荷时间历程,为结构设计、安全性评估提供可靠依据。实例表明,分析结果与实测数据非常接近,所提出的建模方法是可行的。     

乡镇工业电镀行业主要污染物排放量的估算方法

电镀分有氰电镀与无氰电镀两类。乡镇工业电镀行业的环境污染最主要是氰污染和铬污染。本文针对乡镇电镀行业的特点,调查了湖南省沅江市、四川省新都县、湖北省黄陂县、汉川县以及江苏省部分县市乡镇电镀厂,将收集资料及实际监测资料进行回归分析,得出乡镇电镀行业的污染物发生量大小与原材料的消耗有关,可以用多元回归方程估算乡镇电镀行业主要污染物发生量。这一估算方法简单易行,实用可靠,操作方便。     

Heterogeneity in fetal akinesia deformation sequence (FADS): autopsy confirmation in three 20–21-week fetuses

Fetal akinesia deformation sequence (FADS) is a rare condition characterized by intrauterine growth retardation (IUGR), congenital limb contractures, pulmonary hypoplasia, hydramnios and craniofacial abnormalities. The present report comprises an autopsy study of three fetuses to illustrate the variable clinical manifestations and neuropathological findings. Fetus 1 had arthrogryposis and no movement on fetal ultrasound examination. Aborted at 21 weeks, the fetus showed micrognathia, bilateral joint contracture with pterygia at the elbow and axilla. Growth retardation and pulmonary hypoplasia were not major features. Neuropathologic examination revealed anterior horn cell loss and lateral corticospinal tract degeneration in spinal cord, with marked muscular atrophy. Fetus 2, 20 weeks' gestation, had fetal akinesia, nuchal thickening, left pleural effusion, and Dandy-Walker malformation on ultrasound examination. Autopsy showed low-set ears, ocular hypertelorism, cleft palate, flexion contractures with pterygia over axilla, elbow and groin, pulmonary hypoplasia, Dandy-Walker malformation, unremarkable spinal cord and skeletal muscle. Fetus 3, 21 weeks' gestation, was aborted for fetal akinesia, neck and limb webbing and severe arthrogryposis. At autopsy, similar facial abnormalities, contracture and pterygia in neck and multiple major joints were found. Borderline pulmonary hypoplasia and severe lumbar scoliosis were also present. The brain, spinal cord and muscle were unremarkable. In these three fetuses, the prenatal ultrasound and autopsy findings were characteristic of FADS. Neurogenic spinal muscular atrophy was the basis of fetal akinesia in Case 1. Dandy-Walker malformation was present in Case 2, but the pathogenetic mechanism of fetal akinesia was not clear as spinal cord and muscle histology appeared normal. The etiology of akinesia was undetermined in Case 3; no extrinsic or intrinsic cause was identified. Copyright © 2002 John Wiley & Sons, Ltd.     

磷酸盐工业中的放射性污染及其防治

磷矿石中含有一定量的天然放射性物质，在工业生产中，这些物质不仅会在产品、副产品和三废中富集，还会通过生产和使用中的不同环节扩散到周围环境中，对环境造成污染。云南必须对此综合研究，制订措施，使磷化工产业开发与保护并重，可持续发展。     

Ammonia volatilization and availability of Cu, Zn induced by applications of urea with and without coating in soils

Ammonia volatilization and the distribution of Cu and Zn were investigated in two types of soil treated with coated and uncoated urea. The rate of ammonia volatilization in two weeks after fertilizing with coated urea was 8% in soil 1 (soil derived from river alluvial deposits in Dongting Lake Plain) and 5.15% in soil 2 (red soil derived from quaternary red clay), about half the rates observed when fertilizing with common urea, implying that the hydrolysis speed of the coated urea was lower than for common urea, and that the coated urea can increase nitrogen use efficacy. As for the availability of Cu and Zn, their concentrations decreased in the first week after fertilization, and then increased, which was contrary to the effect of treatment on soil pH. For example, when the pH was 7.99, there was 0.79 mg/kg exchangeable Cu and 0.85 mg/kg exchangeable Zn in the soil derived from river alluvial deposits in Dongting Lake Plain. However, the concentrations of exchangeable Cu and Zn were generally lower for the common urea treatments than those with the coated urea because the peak pH for the common urea treatment was greater. The concentrations of these elements correlated well with pH in the range 4-8 in second order polynomial fits.     

Three-dimensional hydrodynamic and water quality model for TMDL development of Lake Fuxian, China

Lake Fuxian is the largest deep freshwater lake in China. Although its average water quality meets Class I of the China National Water Quality Standard (CNWQS), i.e., GB3838-2002, monitoring data indicate that the water quality approaches the Class II threshold in some areas. Thus it is urgent to reduce the watershed load through the total maximum daily load (TMDL) program. A three-dimensional hydrodynamic and water quality model was developed for Lake Fuxian, simulating flow circulation and pollutant fate and transport. The model development process consists of several steps, including grid generation, initial and boundary condition configurations, and model calibration processes. The model accurately reproduced the observed water surface elevation, spatiotemporal variations in temperature, and total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD) concentrations, suggesting a reasonable numerical representation of the prototype system for further TMDL analyses. The TMDL was calculated using two interpretations of the water quality standards for Class I of the CNWQS based on the maximum instantaneous surface and annual average surface water concentrations. Analysis of the first scenario indicated that the TN, TP and COD loads should be reduced by 66%, 68% and 57%, respectively. Water quality was the highest priority; however, local economic development and cost feasibility for load reduction can pose significant issues. In the second interpretation, the model results showed that, under the existing conditions, the average water quality meets the Class I standard and therefore load reduction is unnecessary. Future studies are needed to conduct risk and cost assessments for realistic decision-making.