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841.
842.
Sellek R Jimenez O Aizpurua C Fernandez-Frutos B De Leon P Camacho M Fernandez-Moreira D Ybarra C Carlos Cabria J 《Journal of environmental monitoring : JEM》2008,10(3):362-369
The aim of this study was to develop a specific and highly sensitive method able to detect very low concentrations of Francisella tularensis in soil samples by real-time PCR (qPCR) with SYBR Green I. tul4 gene, which encodes the 17-kDa protein (TUL4) in F. tularensis strains, was amplified using a LightCycler (LC) device. We achieved a detection limit of 0.69 fg of genomic DNA from F. tularensis subp. holarctica live vaccine strain (LVS), corresponding to a value less than 3.4 genome equivalents per reaction. The qPCR was shown to be specific, highly sensitive and reproducible. In addition, we evaluated 2 new methods for recovering bacteria from soil based on 1-step filtration using glass fiber filters and PVDF filters. These filtration methods enabled us to recover F. tularensis efficiently from soil samples. As few as 50 CFU per 0.5 g of soil were detected by qPCR. Capture enzyme-linked immunosorbent assay (cELISA) allowed us to detect and quantify the amount of bacteria recovered from soil by an immunological method. Although qPCR was more sensitive than cELISA, we did not observe substantial differences in the amount of bacteria quantified by both methods. 相似文献
843.
Cairong Lou Hongyu Liu Yufeng Li Yan Peng Juan Wang Lingjun Dai 《Environmental monitoring and assessment》2017,189(11):582
Severe particulate matter (PM, including PM2.5 and PM10) pollution frequently impacts many cities in the Yangtze River Delta (YRD) in China, which has aroused growing concern. In this study, we examined the associations between relative humidity (RH) and PM pollution using the equal step-size statistical method. Our results revealed that RH had an inverted U-shaped relationship with PM2.5 concentrations (peaking at RH = 45–70%), and an inverted V-shaped relationship (peaking at RH = 40 ± 5%) with PM10, SO2, and NO2. The trends of polluted-day number significantly changed at RH = 70%. The very-dry (RH < 45%), dry (RH = 45–60%) and low-humidity (RH = 60–70%) conditions positively affected PM2.5 and exerted an accumulation effect, while the mid-humidity (RH = 70–80%), high-humidity (RH = 80–90%), and extreme-humidity (RH = 90–100%) conditions played a significant role in reducing particle concentrations. For PM10, the accumulation and reduction effects of RH were split at RH = 45%. Moreover, an upward slope in the PM2.5/PM10 ratio indicated that the accumulation effects from increasing RH were more intense on PM2.5 than on PM10, while the opposite was noticed for the reduction effects. Secondary transformations from SO2 and NO2 to sulfate and nitrate were mainly responsible for PM2.5 pollution, and thus, controlling these precursors is effective in mitigating the PM pollution in the YRD, especially during winter. The conclusions in this study will be helpful for regional air-quality management. 相似文献
844.
845.
Alfredo Montero Alvarez Juan R. Estévez Alvarez Clístenes Williams Araújo do Nascimento Iván Pupo González Oscar Díaz Rizo Lázaro Lima Carzola Roberto Ayllón Torres Jorge Gómez Pascual 《Environmental monitoring and assessment》2017,189(1):28
Epiphytic lichens, collected from 119 sampling sites grown over “Roistonea Royal Palm” trees, were used to assess the spatial distribution pattern of lead (Pb) and identify possible pollution sources in Havana (Cuba). Lead concentrations in lichens and topsoils were determined by flame atomic absorption spectrophotometry and inductively coupled plasma (ICP) atomic emission spectrometry, respectively, while Pb in crude oils and gasoline samples were measured by ICP-time of flight mass spectrometry (ICP-ToF-MS). Lead isotopic ratios measurements for lichens, soils, and crude oils were obtained by ICP-ToF-MS. We found that enrichment factors (EF) reflected a moderate contamination for 71% of the samples (EF > 10). The 206Pb/207Pb ratio values for lichens ranged from 1.17 to 1.20 and were a mixture of natural radiogenic and industrial activities (e.g., crude oils and fire plants). The low concentration of Pb found in gasoline (<7.0 μg L?1) confirms the official statement that leaded gasoline is no longer used in Cuba. 相似文献
846.
采用在线单颗粒气溶胶质谱技术源解析方法,对桂林市PM2.5典型排放源的粒径和化学成分进行质谱分析,采集燃煤/燃气源、工业工艺源、扬尘源、油烟源4类共计7个典型排放源。结果表明,桂林市4类排放源细颗粒物的粒径分布为0.25~1.25μm,80%以上的细颗粒分布在0.2~1.0μm的小粒径范围,峰值约0.68μm。细颗粒物离子成分含有Na~+、Mg~+、K~+、NH~+4、Fe~+、Pb~+、Cd~+、V~+、Mn~+、Li~+、Al~+、Ca~+、Cu~+、Zn~+、Cr~+、CN~-、PO_3~-、NO_2~-、NO_3~-、Cl~-、SO_4~(2-)、SiO_3~-等成分,桂林市细颗粒物为元素碳、有机碳元素碳、有机碳、富锰颗粒、富铁颗粒、富钾颗粒、矿物质、左旋葡聚糖以及其他金属等9类。 相似文献
847.
对3类适用于酚类物质的衍生方法进行比较。结果显示,3种衍生方法均具有改善峰形、提高检测灵敏度、降低检出限等优势。其中七氟丁酸酐衍生法具有较大的腐蚀性;TMS衍生体系对水分非常敏感,需确保衍生体系脱水充分;五氟苄基溴衍生相对更成熟和完善。 相似文献
848.
微藻固碳已经成为消减温室气体排放的新的研究热点。利用静态吸收方法,考查了通人纯CO2对普通小球藻生长特点、固定CO2效率以及藻液pH值变异的影响。结果表明:通入纯CO2使小球藻生长延滞期显著延长,比普通培养延长10~12d,对其他生长阶段的影响不大;小球藻固定CO2速率可分为2个过程,即物理固碳过程和生物固碳过程,前者在藻细胞延滞期发生,峰值由CO。溶解于培养液造成,后者在藻细胞生长的指数期、稳定期和衰退期发生,峰值由藻细胞指数生长造成,2个过程中,固定CO2速率的变化趋势都是先增大后降低;纯CO2条件下,藻液pH值变化速率高,4d内,藻液即被酸化,随后藻液pH值变化速率逐渐降低,且pH值稳定在适宜水平。因此,采用小球藻固定高浓度CO2时,建议提高接种量并加强培养前期的pH值监测和调控,以保证藻液保持适宜的pH值,并缩短培养时间,提高生物固碳效率。 相似文献
849.
介绍了2个相同尺寸混凝土扩展基础分别在上拔、上拔与水平力组合荷载作用下的室内足尺试验概况,并根据加载过程中的基顶荷载位移、基础主柱纵筋应变、扩大端钢筋和混凝土应变等试验数据,分析了2种荷载工况下混凝土扩展基础的承载变形特性及混凝土裂缝发展规律。结果表明:①上拔和水平力组合荷载作用下,基础上拔荷载位移曲线呈现出两阶段特性,而水平位移曲线随水平力增加近似呈线性增加,水平荷载降低了扩展基础的抗拔承载性能;②上拔和水平力组合荷载作用下,基础主柱横截面部分受拉、部分承压,在基础立柱与底板连接处的拉应力最大,混凝土裂缝未贯穿全截面,而在上拔荷载作用下,混凝土扩展基础主柱全断面受拉,裂缝贯穿全断面。 相似文献
850.
Sotelo JL Rodríguez AR Mateos MM Hernández SD Torrellas SA Rodríguez JG 《Journal of environmental science and health. Part. B》2012,47(7):640-652
Adsorption has been used to study the removal of atenolol, caffeine, diclofenac and isoproturon, pharmaceutical compounds as emerging contaminants and an endocrine disruptor from ultrapure water and a municipal wastewater treatment plant effluent with three carbonaceous materials: activated carbon, multiwalled carbon nanotubes and carbon nanofibers. The adsorption capacities were studied in the temperature range of 25-65°C and pH range from 3 to 9. Several model isotherms were used to model the adsorption equilibrium data. Also, the competitive adsorption was evaluated. 相似文献