Water-soluble vitamins in natural plankton (copepods) during two consecutive spring blooms compared to vitamins in Artemia franciscana nauplii and metanauplii

Weight-specific contents of asorbic acid, thiamin, riboflavin, niacin, pantothenic acid, vitamin B₆, biotin, folate and vitamin B₁₂ were analysed in natural zooplankton collected from a sea water lagoon in a halibut fry production plant. The samples of natural zooplankton were collected from April/May to the end of June during two consecutive spring blooms. For comparison, the same vitamins were measured in nauplii of Artemia franciscana, just after hatching and after enrichment for 24 and 48 h with a commercial diet. The changes in vitamin content in zooplankton, which consisted mainly of Temora longicornis, during the two spring blooms were <2.5-fold for each specific vitamin. The large number of high quality halibut fry produced using the plankton as a dietary source suggests that the plankton contained sufficient amounts of all water-soluble vitamins. With the exception of thiamin and ascorbic acid, Artemia– both newly hatched and enriched for 24 and 48 h – contained higher levels of water-soluble vitamins than natural zooplankton. Enrichment feeding of Artemia for 48 h stabilised or increased the level of the water-soluble vitamins. Received: 8 March 1999 / Accepted: 21 February 2000     

Fetal laser therapy: applications in the management of fetal pathologies

Fetoscopic coagulation of placental anastomoses is the treatment of choice for severe twin-to-twin transfusion syndrome. In the present day, fetal laser therapy is also used to treat amniotic bands, chorioangiomas, sacrococcygeal teratomas, lower urinary tract obstructions and chest masses, all of which will be reviewed in this article. Amniotic band syndrome can cause limb amputation by impairing downstream blood flow. Large chorioangiomas (>4 cm), sacrococcygeal teratomas or fetal hyperechoic lung lesions can lead to fetal compromise and hydrops by vascular steal phenomenon or compression. Renal damage, bladder dysfunction and lastly death because of pulmonary hypolasia may be the result of megacystis caused by a posterior urethral valve. The prognosis of these pathologies can be dismal, and therapy options are limited, which has brought fetal laser therapy to the forefront. Management options discussed here are laser release of amniotic bands, laser coagulation of the placental or fetal tumor feeding vessels and laser therapy by fetal cystoscopy. This review, largely based on case reports, does not intend to provide a level of evidence supporting laser therapy over other treatment options. Centralized evaluation by specialists using strict selection criteria and long-term follow-up of these rare cases are now needed to prove the value of endoscopic or ultrasound-guided laser therapy. © 2015 John Wiley & Sons, Ltd.     

Development of the German version of the Hospital Survey on Patient Safety Culture: Dimensionality and psychometric properties

Despite the importance of safety climate assessment in healthcare, the psychometric properties of existing safety climate surveys have seldom been tested. We developed a German version of the HSOPSC and investigated its dimensionality and predictive validity. In adapting the survey we differentiated between the two management levels (hospital, unit) by duplicating dimensions of the HSOPSC. Based on a sample of n = 568, confirmatory and exploratory factor analyses (CFA, EFA) were applied. Relationships between safety climate and outcome variables were investigated by regression analyses. CFA did not replicate the proposed factor structure, supporting evidence from other European countries. EFAs revealed eight factors (instead of twelve proposed for the original instrument) and allowed for differentiating between management levels (hospital, unit). For physicians and nurses, different predictors were identified for overall safety climate perception. Further cross-cultural investigations of dimensionality are necessary for international comparisons of safety climate in healthcare. Our results highlight the importance of capturing the impact of different management levels, especially in large hospitals, and differences between professional groups in order to design interventions accordingly.     

Risk assessment and land-use planning regulations in France following the AZF disaster

After the disaster of AZF plant in Toulouse on 21 September 2001 (31 people killed, 3000 injured and 3 billion dollars of damage), France adopted a new law relative to safety reports and land-use planning on 30 July 2003. This law asks for the investigation of all representative scenarios and the assessment of their probabilities to demonstrate the acceptable level of safety of an industrial facility. Therefore significant changes were introduced in the way of doing risk analysis in France and some difficulties were found for the implementation of a probabilistic approach.This paper presents the new approach of risk analysis established by the French Ministry of the Environment, and particularly focuses on:? the benefits and limits of the semi-quantitative probabilistic assessment method;? the benefits and difficulties to use a quantitative probabilistic assessment method;? some learning from the risk analysis approaches carried out in the nuclear industry;? some discussion about the national matrix to appreciate the gravity of human consequences from an accident outside facilities.     

Engine Performance and Emission Characteristics of Plastic Oil Produced from Waste Polyethylene and Its Blends with Diesel Fuel

The overall objective of this study was to explore the utility of waste plastics as a potential source of diesel fuel. An experimental study was conducted to evaluate the use of various blends of plastic oil produced from waste polyethylene (WPE) with diesel fuel (D). WPE was degraded thermally and catalytically using sodium aluminum silicate as a catalyst. The oil collected at optimum conditions (414°C–480°C range and 1 h reaction time) was fractionated at different temperatures and fuel properties of the fractions were measured. Plastic oil was blended with diesel fuel at the volumetric ratios of 5%, 10%, 15%, 20%, and 100%. Fuel properties of blends are found comparable with those of diesel fuel within the EN 590 Diesel Fuel standard and they can also be used as fuel in compression ignition engines without any modification. Engine performance and exhaust emission studies of 5% WPE-D (WPE5) blend were performed. Experimental results showed that carbon monoxide (CO) emission is decreased by 20.63%, carbon dioxide (CO₂) emission is increased by 3.34%, and oxides of nitrogen (NO_x) emission is increased by 9.17% with WPE-D (WPE5) blend compared to diesel fuel.     

Success and failure assessment methodology for wastewater and faecal sludge treatment projects in low-income countries

Many factors influence success and failure of large-scale faecal sludge and wastewater treatment projects in low-income countries. Benchmarking indicators and multicriteria analysis were adapted to define key institutional, technical and financial factors, to analyse their interrelations, and understand priorities to consider when planning and managing treatment plants. For the first time, these methods have been combined in a quantitative manner to assess planned and on-going treatment plant projects. This new methodology will aid sanitation utilities, private consultants, and funding institutions to prioritise activities and organise the operation of treatment plants.     

Classification of Ephemeral,Intermittent, and Perennial Stream Reaches Using a TOPMODEL‐Based Approach

Whether a waterway is temporary or permanent influences regulatory protection guidelines, however, classification can be subjective due to a combination of factors, including time of year, antecedent moisture conditions, and previous experience of the field investigator. Our objective was to develop a standardized protocol using publically available spatial information to classify ephemeral, intermittent, and perennial streams. Our hypothesis was that field observations of flow along the stream channel could be compared to results from a hydrologic model, providing an objective method of how these stream reaches can be identified. Flow‐state sensors were placed at ephemeral, intermittent, and perennial stream reaches from May to December 2011 in the Appalachian coal basin of eastern Kentucky. This observed flow record was then used to calibrate the simulated saturation deficit in each channel reach based on the topographic wetness index used by TOPMODEL. Saturation deficit values were categorized as flow or no‐flow days, and the simulated record of streamflow was compared to the observed record. The hydrologic model was more accurate for simulating flow during the spring and fall seasons. However, the model effectively identified stream reaches as intermittent and perennial in each of the two basins.     

Determination of phosphorus speciation in dairy manure using XRD and XANES spectroscopy

Intensive manure application is an important source of diffuse phosphorus (P) pollution. Phosphorus availability from animal manure is influenced by its chemical speciation. The major objective of this study was to investigate the P speciation in raw and anaerobically digested dairy manure with an emphasis on the calcium (Ca) and magnesium (Mg) phosphate phases. Influent and effluent from an on-farm digester in Wisconsin were sampled and sieved, and the 25 to 53 microm size fraction was dried for X-ray powder diffraction (XRD) and P K-edge X-ray absorption near edge structure (XANES) analyses. Struvite (MgNH4PO4.6H2O) was identified in both the raw (influent) and anaerobically digested (effluent) manure using XRD. Qualitative analysis of P K-edge XANES spectra indicated that the Ca orthophosphate phases, except dicalcium phosphate anhydrous (DCPA) or monetite (CaHPO4), were not abundant in dairy manure. Linear combination fitting (LCF) of the P standard compounds showed that 57.0 and 43.0% of P was associated with DCPA and struvite, respectively, in the raw manure. In the anaerobically digested sample, 78.2% of P was present as struvite and 21.8% of P was associated with hydroxylapatite (HAp). The P speciation shifted toward Mg orthophosphates and least soluble Ca orthophosphates following anaerobic digestion. Similarity between the aqueous orthophosphate (aq-PO4), newberyite (MgHPO4.3H2O), and struvite spectra can cause inaccurate P speciation determination when dairy manure is analyzed solely using P K-edge XANES spectroscopy; however, XANES can be used in conjunction with XRD to quantify the distribution of inorganic P species in animal manure.     

Thioredoxins in bacteria: functions in oxidative stress response and regulation of thioredoxin genes

Thioredoxins fulfill a number of different important cellular functions in all living organisms. In bacteria, thioredoxin genes are often regulated by external factors. In turn, thioredoxins influence the expression of many other genes. The multiple and important functions of thioredoxins in cells necessitate to appropriately adjust their level. This review outlines different strategies that have evolved for the regulation of bacterial thioredoxin genes. It also summarizes effects of thioredoxins on gene regulation and presents a recent model for a redox-dependent gene regulation that is mediated by thioredoxins.