A revisit of trisomy 20 mosaicism in prenatal diagnosis—an overview of 103 cases

One hundred and three cases with prenatal diagnosis of trisomy 20 mosaicism through amniocentesis were reviewed. Approximately 90 per cent (90/101) of the cases were associated with grossly normal phenotype. It is likely that, in the majority of cases, cells with trisomy 20 were extraembryonic in origin or largely confined to the placenta. However, in some cases, the cells with trisomy 20 were confined to certain specific fetal organs or tissues such as kidney, skin, etc. Cytogenetic follow-up studies in liveborns should include a culture from urine sediment.     

Population genetics of the blue crabCallinectes sapidus: modest population structuring in a background of high gene flow

To determine the genetic population structure of blue crabs (Callinectes sapidus Rathbun), electrophoretic allozyme analysis was performed on 750 individuals collected from 16 nearshore locations ranging from New York to Texas, USA. Twenty enzymes and non-enzymatic proteins coded by 31 presumptive loci were examined. Twenty-two loci were either monomorphic or polymorphic at less than theP ₉₅ level; alleles for these polymorphic loci were geographically dispersed. Allele frequencies for three of the remaining polymorphic loci were homogeneous over all populations, as were levels of polymorphism and heterozygosity. Phenograms generated by the UPGMA (unweighted pair-group method using arithmetic averages) and distance Wagner methods exhibited no geographic pattern in the clustering of populations. Estimates ofN _em (effective number of migrants per generation between populations) indicated substantial gene flow, with aalues sufficiently high to infer panmixia between all blue crab populations from New York to Texas. However, despite this high level of gene flow, two striking patterns of geographic differentiation occurred: genetic patchiness and clinal variation. Allele frequencies atEST-2, GP-1, IDHP-2, DPEP-1, DPEP-2, andTPEP exhibited genetic patchiness on local and range-wide geographic scales, and allele frequencies atEST-2 varied temporally. Genetic patchiness in blue crabs is likely to be the result of the pre-settlement formation and subsequent settlement of genetically heterogeneous patches of larvae; allele frequencies of those larval patches may then be further modified through ontogeny by localized selection. In the Atlantic Ocean, a regional latitudinal cline ofEST-2 allele frequencies was superimposed on the range-wide genetic patchiness exhibited by that locus. This pattern against a background of high gene flow is highly likely to be maintained by selection. In estuaries along the Atlantic Ocean coast, a combination of low adult long-distance migration and a high retention rate of locally spawned larvae could serve to segregate populations and allow for the development of the geographic cline inEST-2. The Gulf of Mexico showed no apparent cline, perhaps due to long-distance migration of females in some regions of the Gulf, or to masking by genetic patchiness. These results emphasize the importance of both ecological and evolutionary time scales and structuring mechanisms in determining genetic population structure.     

Aggregation of Adenovirus 2 in Source Water and Impacts on Disinfection by Chlorine

It is generally accepted that viral particles in source water are likely to be found as aggregates attached to other particles. For this reason, it is important to investigate the disinfection efficacy of chlorine on aggregated viruses. A method to produce adenovirus particle aggregation was developed for this study. Negative stain electron microscopy was used to measure aggregation before and after addition of virus particles to surface water at different pH and specific conductance levels. The impact of aggregation on the efficacy of chlorine disinfection was also examined. Disinfection experiments with human adenovirus 2 (HAdV2) in source water were conducted using 0.2 mg/L free chlorine at 5 °C. Aggregation of HAdV2 in source water (≥3 aggregated particles) remained higher at higher specific conductance and pH levels. However, aggregation was highly variable, with the percentage of particles present in aggregates ranging from 43 to 71 %. Upon addition into source water, the aggregation percentage dropped dramatically. On average, chlorination CT values (chlorine concentration in mg/L × time in min) for 3-log₁₀ inactivation of aggregated HAdV2 were up to three times higher than those for dispersed HAdV2, indicating that aggregation reduced the disinfection rate. This information can be used by water utilities and regulators to guide decision making regarding disinfection of viruses in water.     

The Issue of No Moral Agency in Climate Change

The dominant methodological assumptions in climate ethical debates are rational-individualistic. The aim of this paper is to examine whether the rational-individualistic methodological framework is compatible with a theory of moral responsibility for climate change. I employ three fitness criteria of moral agency: (1) a normatively significant choice, (2) sufficient knowledge and (3) control. I demonstrate that the rational-individualistic methodology does not provide a framework in which rational agents meet the three criteria. I conclude that rational-individualistic agents are not fit to be held morally responsible for climate change. The paper demonstrates that the dominant climate-ethical view rests on a methodology that does not allow for a conceptualisation of a moral agent of climate change.     

PESTICIDE ACCUMULATION IN A NEW IMPOUNDMENT IN IOWA1

ABSTRACT: Estimates were made of the amounts of selected pesticides entering and leaving Saylorville Reservoir, a new partly filled impoundment on the Des Moines River. Samples were collected at 1– or 2-week intervals at stations above, in, and below the reservoir. Atrazine, alachlor, and cyanazine were found only in the dissolved state, and dieldrin and p, p′ -DDE in both the, dissolved and suspended state. Heptachlor epoxide, p,p-DDT. p, p′ -DDD, 2,4-D, 2,4,5-TP, endrin, lindane, methoxychlor, propachlor, and toxaphene were not detected or were at very low concentrations. Estimated deposition in the reservoir from September 1977 to October 1978 was 281 kg atrazine, 251 kg alachlor, 26 kg cyanazine, 16 kg dieldrin, and 20 kg p, p′ -DDE.     

“The Kesterson effect”

Hypothesized to be derived from Cretaceous marine sedimentary rocks, selenium contamination of the Kesterson National Wildlife Refuge is traced through irrigation drainage to the source bedrock of the California Coast Ranges. This biogeochemical pathway of selenium is defined here as the “Kesterson effect.” At the refuge ponds, this effect culminated in 1983 in a 64% rate of deformity and death of embryos and hatchlings of wild aquatic birds. From the previous companion paper on irrigation drainage, the Kesterson effect has been implicated in nine of 11 reconnaissance areas studied in the western United States. Deformities have resulted in at least five of these sites. Climatic, geologic, hydrologic, and soil conditions in these reconnaissance areas are similar to those in the area surrounding Kesterson National Wildlife Refuge in the west-central San Joaquin Valley of California. In California, selenium, as selenate, was ultimately found weathered with sulfur from marine sources in soluble sodium and magnesium sulfate salts, which are concentrated by evaporation on farmland soils. The Se, mobilized by irrigation drainage, is bioaccumulated to toxic levels in refuge wetland ponds that are located mainly in hydrologically closed basins and thus act as concentrating disposal points. The depositional environment of the ponds may be similar to that of the nutrient-rich continental shelf edge and slope in which Cretaceous, Eocene, and Miocene sediments found to be seleniferous in the California Coast Ranges were deposited. Bioaccumulation may be therefore a primary mechanism of selenium enrichment in ancient sediments in addition to that of the formerly suggested Cretaceous volcanic pathway.     

A Profile and Management of the US Army's underground storage tanks

The US Army owns more than 10,000 underground storage tanks (USTs), many of which are old and may be leaking. The Resource Conservation and Recovery Act of 1976 required tank owners to collect and report data on them by May 1986. In order to manage the large amounts of information on its USTs, the Army developed a microcomputer-based data base system. The data base system is user friendly and allows the user to store, organize, and manipulate the UST data. A leak potential index (LPI) was also developed and calculated for each of the Army's USTs. The LPI is used to prioritize USTs so that those with higher LPIs can be monitored closely. A characteristic profile of Army USTs according to construction material, capacity, age, content, and LPI is presented in this paper.The opinions expressed in this paper are those of the authors and do not necessarily reflect the views of the US Department of the Army, nor does the mention of trade names or commercial products constitute endorsement or recommendation for use.