Contaminant Exposure of Barn Swallows Nesting on Bayou D'Inde, Calcasieu Estuary, Louisiana, USA

Current and historical point source discharges, storm water runoff, and accidental spills have contaminated the water, sediment, and biota within the Calcasieu Estuary in southwestern Louisiana. In 2003, barn swallow (Hirundo rustica) eggs and nestlings were collected beneath two bridges that cross Bayou d′Inde, the most contaminated waterway within the Calcasieu Estuary. Samples were also collected from a bridge over Bayou Teche, a reference site in south central Louisiana. Polychlorinated biphenyl (PCB) concentrations in barn swallow eggs and nestlings were significantly higher at the downstream site on Bayou d′Inde (2.8 μ g/g PCBs in eggs and 1.5 μ g/g PCBs in nestlings) than at the other two sites (< 0.2 μ g/g PCBs in eggs and nestlings at both sites). Ethoxyresorufin-O-dealkylase activity in nestling livers was significantly higher at the downstream site on Bayou d′Inde (50 pmol/min/mg) compared to the other two locations (24 pmol/min/mg, each), probably because of exposure to PCBs. Polychlorinated dibenzo-p-dioxin and polychlorinated dibenzofuran concentrations in eggs and polycyclic aromatic hydrocarbons in nestlings were at background concentrations at all sites. Trace element concentrations in barn swallow eggs and nestling livers were at background levels and did not differ among the three sites. A biomarker of DNA damage did not differ among sites.     

PCB Levels and Trends Within the Detroit River-Western Lake Erie Basin: A Historical Perspective of Ecosystem Monitoring

An international workshop held in the spring of 2002 convened a group of technical experts to address monitoring, modeling, and management of PCBs within the Detroit River-Western Lake Erie basin. Participants shared and discussed a diverse set of research data bases pertaining to PCB levels within the region, discussed observed changes within different components of the local ecosystem, and identified several primary issues impacting future PCB management strategies. Results presented at the workshop indicate dramatic reductions in PCB contamination levels have been observed in much of study area between the late 1970s and mid-1990s. Estimates of loadings attributable to water and atmospheric sources have generally declined, as have PCB concentrations in herring gull eggs, smelt and walleye. Nevertheless, additional improvements have not been observed during recent years and elevated contamination levels remain a concern within local hot spots, particularly in the lower Detroit River and Trenton Channel. A primary recommendation broadly supported by workshop participants is the need to maintain, support, and coordinate a comprehensive ecosystem monitoring program for the Detroit River-Western Lake Erie basin, one that incorporates both near-field and far-field monitoring elements. Such a program is crucial to provide necessary data in support of understanding ecosystem trends, calculating annual mass loadings to the system, assessing impacts of remediation actions, developing improved modeling frameworks, and formulating cost-effective management strategies for the future.     

Investigation of sequential and enzymatic extraction of arsenic from drinking water distribution solids using ICP-MS

A sequential extraction approach was utilized to estimate the distribution of arsenite [As(iii)] and arsenate [As(v)] on iron oxide/hydroxide solids obtained from drinking water distribution systems. The arsenic (As) associated with these solids can be segregated into three operationally defined categories (exchangeable, amorphous and crystalline) according to the sequential extraction literature. The exchangeable As, for the six drinking water solids evaluated, was estimated using 10 mM MgCl(2) and 10 mM NaH(2)PO(4) and represented between 5-34% of the total As available from the solid. The amorphously bound As was estimated using 10 mM (NH(4))(2)C(2)O(4) and represented between 57-124% of the As available from the respective solid. Finally, the crystalline bound As was estimated using titanium citrate and this represented less than 1.5% of the As associated with the solids. A synthetic stomach/intestine extraction approach was also applied to the distribution solids. The stomach fluid was found to extract between 0.5-33.3 microg g(-1) As and 120-2,360 microg g(-1) iron (Fe). The As concentrations in the intestine fluid were between 0.02-0.04 microg g(-1) while the Fe concentration ranged from 0.06-0.7 microg g(-1) for the first six drinking water distribution solids. The elevated Fe levels associated with the stomach fluid were found to produce Fe based precipitates when the intestinal treatment was applied. Preliminary observations indicate that most of the aqueous Fe in the stomach fluid is ferric ion and the observed precipitate produced in the intestine fluid is consistent with the decreased solubility of ferric ion at the pH associated with the intestine.     

Persistent organic pollutants in European background air: derivation of temporal and latitudinal trends

Data are presented for polynuclear aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), polybrominated diphenyls ethers (PBDEs) and selected organochlorine compounds (OCs) in passive air samplers (PAS) along a rural/remote latitudinal transect from southern UK to northern Norway during 2002-2004. This study is part of an ongoing campaign, using semi-permeable membrane devices (SPMDs) as PAS over two year intervals since 1994. Data for PCBs, selected OCs and PBDEs are compared with that from previous campaigns. Absolute sequestered amounts of selected PCB congeners have decreased in a first order fashion between 1994-2004, with an average atmospheric clearance rate of 4.1 +/- 0.6 years and continue to fractionate with latitude. HCB has also declined between 1998-2004, with a clearance rate of 6 +/- 2.4 years. Data on DDT and its breakdown products indicate little fresh release in Europe. Comparison of PBDEs in 2000-02 and 2002-04 indicates site differences, generally with increases at UK sites and decreases in Norway. BDE-28, 47 and 49 decreased with increasing latitude (p < 0.04), while the other congeners did not show any significant latitudinal dependence. Transect data are presented for PAHs the first time. Three- and 4-ringed compounds dominated the mixture present in the SPMD. The PAH composition of the SPMDs at site 3 was compared to the average composition taken by active sampling at the same site. SPMD performance for sampling PAHs leaves many uncertainties, but they can be successfully used to semiquantitatively detect PAHs in the atmosphere. Fluorene and phenanthrene increased with latitude (p > 0.05), while 1-methylphenanthere, fluoranthene, benzo[b]fluoranthene and indeno[123-cd]pyrene decreased. Results are discussed in terms of sources, long-range atmospheric transport, global fractionation and clearance processes.     

An FTIR investigation of isocyanate skin absorption using in vitro guinea pig skin

Isocyanates may cause contact dermatitis, sensitization and asthma. Dermal exposure to aliphatic and aromatic isocyanates can occur in various exposure settings. The fate of isocyanates on skin is an important unanswered question. Do they react and bind to the outer layer of skin or do they penetrate through the epidermis as unreacted compounds? Knowing the kinetics of these processes is important in developing dermal exposure sampling or decontamination strategies, as well as understanding potential health implications such exposure may have. In this paper the residence time of model isocyanates on hairless guinea pig skin was investigated in vitro using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometry. Model isocyanates tested were octyl isocyanate, polymeric hexamethylene diisocyanate isocyanurate (pHDI), polymeric isophorone diisocyanate isocyanurate (pIPDI) and methylenediphenyl diisocyanate (MDI). Isocyanates in ethyl acetate (30 microL) were spiked directly on the skin to give 0.2-1.8 micromol NCO cm(-2) (NCO = -N=C=O), and absorbance of the isocyanate group and other chemical groups of the molecule were monitored over time. The ATR-FTIR findings showed that polymeric isocyanates pHDI and pIPDI may remain on the skin as unreacted species for many hours, with only 15-20% of the total isocyanate group disappearing in one hour, while smaller compounds octyl isocyanate and MDI rapidly disappear from the skin surface (80+% in 30 min). Isocyanates most likely leave the skin surface by diffusion predominantly, with minimal reaction with surface proteins. The significance of these findings and their implications for dermal exposure sampling and isocyanate skin decontamination are discussed.     

Societal implications of a changing Arctic Ocean

The Arctic Ocean is undergoing rapid change: sea ice is being lost, waters are warming, coastlines are eroding, species are moving into new areas, and more. This paper explores the many ways that a changing Arctic Ocean affects societies in the Arctic and around the world. In the Arctic, Indigenous Peoples are again seeing their food security threatened and cultural continuity in danger of disruption. Resource development is increasing as is interest in tourism and possibilities for trans-Arctic maritime trade, creating new opportunities and also new stresses. Beyond the Arctic, changes in sea ice affect mid-latitude weather, and Arctic economic opportunities may re-shape commodities and transportation markets. Rising interest in the Arctic is also raising geopolitical tensions about the region. What happens next depends in large part on the choices made within and beyond the Arctic concerning global climate change and industrial policies and Arctic ecosystems and cultures.     

Dams and Flows: Immersing in Western Meaning Systems in Search of Ecocultural Reflexivity

In this unprecedented era of anthropogenic ecological destruction, this study illustrates inadequacies in conceptual language available in Western settings to think deeply and holistically about “nature.” At the same time, the study illustrates transformative potential of moments of ecocultural reflexivity. Using free write methodology, we examine ways participants in the United States, New Zealand, and Australia articulate what they mean when they say “nature.” We interpret participant streams of consciousness as representative of a wider Western river-way, a channel of dominant, multiple, and contradictory meanings in continuous movement. We identify conceptual obstructions that provide glimpses into ways Western ecological relations are bounded and dammed by binary, fragmented, and unconsidered meanings. Yet reflexivity in the face of such obstructions, and in potent ecocultural side streams of childhood remembering and ecocentric cosmology, provides some participants a lucid flow of regenerative narratives at a time such shared stories are urgently needed.     

Examination of the influence of dissolved halite (NaCl) on the leaching of lead (Pb) from cement-based solidified wastes

The leaching of lead from cement-based solidified waste forms mixed at different water/cement ratios was studied by conducting equilibrium and semi-dynamic leaching tests using deionized water and sodium chloride solutions. The results suggest that leaching of the primary constituents of the cement (calcium, silicon and sulfate) is controlled by solubility equilibria, with increased leaching into chloride solutions due to ionic strength effects. The original porosity of the waste forms increased with water/cement ratio and chloride solutions further increased it as a result of decalcification. Lead leaching was generally low, and appears to be a transport-controlled process, such that leaching correlates positively with porosity.     

Structural insights on bioremediation of polycyclic aromatic hydrocarbons using microalgae: a modelling-based computational study

Research on bioremediation of polycyclic aromatic hydrocarbons (PAHs) has established that several remediating microbial species are capable of degrading only low molecular weight (LMW)-PAHs, whereas high molecular weight (HMW)-PAHs are hardly degradable. In the present study, the efficiency of degradation of both LMW and HMW-PAHs by cytochrome P450 monooxygenase (CYP) of microalgae was studied. CYP have a key role in the detoxification of xenobiotics. So far, the structure of CYP in microalgae is not predicted; the protein structure was constructed by molecular modelling in the current study using the available template of microalgal CYP. Modelled microalgae 3D structures were docked against 38 different PAH compounds, and the information regarding the interaction between protein and PAHs viz. binding sites along with mode of interactions was investigated. We report that CYP from the microalgae Haematococcus pluvialis and Parachlorella kessleri was found to possess broad oxidising capability towards both LMW and HMW-PAHs. P. kessleri showed a least value with extra precision glide score of ? 10.23 and glide energy of ? 23.48 kcal/mol. PAHs bind to CYP active sites at Lys ⁶⁹, Trp ⁹⁶, Gln ³⁹⁷ and Arg ³⁹⁸ through intermolecular hydrogen bonding. Also, study revealed that PAHs interacted with CYP active sites through intermolecular hydrogen bonding, hydrophobic bonding, π–π interactions and van der waals interactions. Thus, structural elucidation study confirms that microalgae Parachlorella kessleri have the capacity to remediate HMW more efficiently than other microorganisms. Our results provide a framework in understanding the structure and the possible binding sites of CYP protein for degradation of PAH and that could be a screening tool in identifying the phycoremediation potential.