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221.
Noninvasive prenatal diagnosis for Duchenne muscular dystrophy based on the direct haplotype phasing
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认识化学品的毒性机制是开展化学品健康风险评估的基础。掌握化学品有害效应的遗传易感性机制是开展精准的健康风险评估的前提。传统的毒理基因组学主要分析化学品暴露诱导的组学表达图谱,不能建立生物学表型与特定基因/通路表达的直接关联。功能基因组学通过敲除或者敲降全基因组或者特定的基因集,建立基因-化学品毒性的直接关联,进而研究化学品致毒的过程和机制,同时可以提供与化学品暴露的遗传易感性相关的分子响应信息。本论文综述了功能基因组学技术的原理及其主要发展,介绍了酵母、鸡DT40细胞和RNA干扰等功能基因组学测试方法的优缺点。同时,详述了CRISPR功能基因组学的原理和特点,以及其在化学品毒性机制研究中的应用,展望了联合应用分子流行病学和CRISPR功能基因组学,开展化学品有害效应的易感性机制研究。 相似文献
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The current status of ecotourism in rural China was analyzed in this paper.Empirical surveys covering the whole country indicate that ecotourism in rural China has attracted a large number of tourists who,however,didn’t generate revenues that can match the number.Although the environmental quality of those rural ecotourism destinations is high with little negative impacts,several problems have already appeared,suggesting a need to monitor those areas.The current practice of interpretation in most rural ecotourism destinations did not provide environmental education opportunities to the tourists.And local communities need more effective ways to decide on the prospects of local ecotourism development by themselves.Finally,a few recommendations for improving the sustainability of ecotourism destinations were provided. 相似文献
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He Zekang Han Dongmei Song Xianfang Yang Shengtian 《Environmental science and pollution research international》2020,27(30):37592-37613
Environmental Science and Pollution Research - Overexploitation of groundwater has resulted in seawater intrusion in many semiarid and arid coastal areas. This study illustrates the origin of... 相似文献
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Guohua Su Yelin Huang Fengxiao Tan Xiaowei Ni Tian Tang Suhua Shi 《Marine Biology》2007,150(3):321-328
Mangrove forests, with their ecological significance and economic benefits, are vital inter-tidal wetland ecosystems. Lumnitzera littorea (Combreataceae) is a non-viviparous mangrove distributed in tropical Asia and North Australia. Due to natural and human impacts, populations of this species have been isolated, fragmented, and highly disturbed. In China, L. littorea is an endangered species, restricted to small regions of Hainan Island. The genetic composition of five populations of this species from the Indo-West Pacific (South China, Malay Peninsula, Sri Lanka, North Australia) was assessed using inter simple sequence repeat (ISSR) makers. At the species level, expected mean heterozygosity (He) was 0.240 with 75.6% of loci polymorphic (P). However, genetic variation was much lower at the population level (P = 37.1%, He = 0.118). A high coefficient of gene differentiation (Gst = 0.515) and low level of gene flow (Nm = 0.470) indicated significant genetic differentiation among populations. AMOVA also indicated that more than half the total variation (58.4%) was partitioned among populations. The high degree of differentiation observed among populations emphasizes the need for appropriate conservation measures that incorporate additional populations into protected areas, and achieve the restoration of separate, degraded populations. 相似文献