Genomic erosion in a demographically recovered bird species during conservation rescue

The pink pigeon (Nesoenas mayeri) is an endemic species of Mauritius that has made a remarkable recovery after a severe population bottleneck in the 1970s to early 1990s. Prior to this bottleneck, an ex situ population was established from which captive-bred individuals were released into free-living subpopulations to increase population size and genetic variation. This conservation rescue led to rapid population recovery to 400–480 individuals, and the species was twice downlisted on the International Union for the Conservation of Nature (IUCN) Red List. We analyzed the impacts of the bottleneck and genetic rescue on neutral genetic variation during and after population recovery (1993–2008) with restriction site-associated sequencing, microsatellite analyses, and quantitative genetic analysis of studbook data of 1112 birds from zoos in Europe and the United States. We used computer simulations to study the predicted changes in genetic variation and population viability from the past into the future. Genetic variation declined rapidly, despite the population rebound, and the effective population size was approximately an order of magnitude smaller than census size. The species carried a high genetic load of circa 15 lethal equivalents for longevity. Our computer simulations predicted continued inbreeding will likely result in increased expression of deleterious mutations (i.e., a high realized load) and severe inbreeding depression. Without continued conservation actions, it is likely that the pink pigeon will go extinct in the wild within 100 years. Conservation rescue of the pink pigeon has been instrumental in the recovery of the free-living population. However, further genetic rescue with captive-bred birds from zoos is required to recover lost variation, reduce expression of harmful deleterious variation, and prevent extinction. The use of genomics and modeling data can inform IUCN assessments of the viability and extinction risk of species, and it helps in assessments of the conservation dependency of populations.     

Ultrasonographic features in a case of heterozygous achondroplasia at 25 weeks' gestation

Prenatal diagnosis of heterozygous achondroplasia at 25 weeks is described. First-level fetal ultrasonography demonstrated short long bones of the lower limbs. Second-level examination showed a large head; shortened femur, fibula, and tibia; a ‘trident’-shaped hand; and an abnormal facial profile.     

Global pilot study for persistent organic pollutants (POPs) using PUF disk passive air samplers

Polyurethane foam (PUF) disks were deployed at global background sites, to test logistical issues associated with a global monitoring network for persistent organic pollutants (POPs). alpha-HCH, exhibited relatively high and uniform concentrations (17-150 pg/m3) at temperate and arctic sites with elevated concentrations associated with trans-Pacific inflow. Concentrations were much lower (<5 pg/m3) in Bermuda, Chile and Cape Grim. Concentrations for gamma-HCH, the main component of lindane, were spatially similar to the alpha-HCH pattern but lower in magnitude (typically, <10 pg/m3). Chlordane concentrations (sum of cis-chlordane, trans-chlordane and trans-nonachlor) were also low (<10 pg/m3). Dieldrin concentrations were in the range 2-25 pg/m3 at most sites but elevated in Bermuda. Back trajectories suggest that advection from Africa and the US may contribute. Endosulfan, a popular current-use pesticide, exhibited highest concentrations ranging from tens to hundreds of pg/m3. There was good agreement between duplicate samplers at each site and PUF disk-derived air concentrations agreed with high volume data. Few logistical/analytical problems were encountered in this pilot study.     

Contamination of wild-grown edible mushrooms by heavy metals in a former mercury-mining area

The aim of this study was to evaluate the contamination of six edible wild species of mushrooms (Boletus pulverulentus, Cantharellus cibarius, Lactarius quietus, Macrolepiota procera, Russula xerampelina and Suillus grevillei) by heavy metals (Hg, Cd, Pb, Zn, Cu, Ni, Cr, Co, Mn and Fe). Mushroom samples were collected from sites contaminated by emissions from mining and processing of polymetallic ores in operation during the period 1969–1993 in Rudňany, southeast Slovakia. The four study sites spanned up to a 5-km distance from the emission source. The collected mushroom samples were analyzed using Flame Atomic Absorption Spectrophotometry and/or Flame Atomic Absorption Spectrophotometry with graphite furnace. Mercury, Cd and, in some samples, also Pb present the highest risks in terms of contamination of the food chain following subsequent consumption. The content of two metals in the dry matter (dm) of the mushrooms exceeded the limits set by the European Union (EU; Cd: 0.5 mg/kg dm, Pb: 1.0 mg/kg dm). The highest mean contents of the eight metals recorded for S. grevillei were 52.2, 2.15, 107, 104, 2.27, 2.49, 81.6 and 434 mg/kg dm for Hg, Pb, Zn, Cu, Ni, Cr, Mn and Fe, respectively. The highest content of Cd was recorded in M. procera (3.05 mg/kg dm) and that of Co in L. quietus (0.90 mg/kg dm). The calculated weekly intake for Hg, Pb and Cd shows that regular consumption of mushrooms from the studied area poses risks to human health.     

Assessing the completeness of a job analysis procedure

The current research analyzed employee perceptions of the completeness of a self-report, point-factor job evaluation system. Complementary qualitative and quantitative techniques were used to identify characteristics of jobs, characteristics of employees, and characteristics of the job evaluation procedure that affected employee perceptions of the system. Analyses indicated that the job evaluation instrument might describe some classes of jobs more completely than others. Situations where job evaluation is particularly problematic are identified and discussed.     

Distribution of long-lived radionuclides of the 238U series in the sediments of a small river in a uranium mineralized region of Spain

A study is presented on the distribution and mobilization of the natural U isotopes (238U and 234U), 230Th, and 226Ra in the sediments of a small river crossing an uranium mineralized zone where a disused uranium mine is located. Due to the preferential directions for surface run-off waters and to the mine's situation, one sampling point along the river bed was identified as a point of accumulation of radionuclides. The average values of the activity concentrations (Bq/kg) in this sediment sample were 5,025, 5,055, 5,915 and 1,694 for 238U, 234U, 230Th and 226Ra, respectively, while the respective average values of the activity concentrations (Bq/kg) for the sediment sample considered to give the background level were 125, 124, 131 and 370. Isotopic ratios between the descendants of 238U served to clarify some paths of distribution, involving the soils nearest to the sampling points and the location of these points with respect to the disused mine. The differences in behaviour found between the uranium, thorium and radium isotopes were associated to the mobility of these radionuclides in the fluvial system studied. Correlations between the radionuclide activity concentration ratios and stable element concentrations in the sediment samples were also investigated.     

Risk perception and trust in the context of urban brownfields

Data are reported from a postal questionnaire completed by 747 residents of two urban local authority areas within which there were sites of brownfield land with significant levels of contamination. Respondents rated their perceptions of the extent to which their neighbourhood and own home were relatively vulnerable to contamination, their concern about possible effects of contamination, their satisfaction with their council in terms of consultation with residents on housing and development issues, and their trust in their council with respect to contaminated land risks. Satisfaction with, and trust in, the council was generally low in both areas, and especially so among those who perceived themselves to be more vulnerable to contamination. Nonetheless, dissatisfaction was less marked in the area where the local authority, according to background information, had pursued a more open and proactive style of risk communication and consultation with residents. The main predictors of trust, across both areas, were perceptions that the council was openly prepared to tell residents what they knew, and that the council had residents’ interests at heart. Implications are discussed for the impact of different modes of risk communication on trust.     

monitoring bioaccumulation of contaminants in the belted kingfisher (ceryle alcyon)

The belted kingfisher (Ceryle alcyon), acommon piscivore in the eastern United States, hasbecome a common endpoint in ecological riskassessments (ERA) because of their high consumptionof potentially contaminated aquatic prey. Whilebioaccumulation data and biosurveys may be used tosupport conclusions of ERAs for kingfishers, thereare currently no published data on contaminantconcentrations in kingfishers. Additionally,methods available for collecting biological samples(e.g., feathers, eggs, food debris, etc.) fromkingfisher burrows can be detrimental to thereproductive success of the birds. We present amethod for obtaining samples from burrows during orfollowing the nesting season. The method wasapplied to kingfisher burrows on the Oak RidgeReservation (ORR) in eastern Tennessee. Feathers,eggshells, and nestlings were collected from burrows and analyzed. In addition, carcasses ofthree adult kingfishers found dead on the ORR wereanalyzed. Metals and radionuclides were accumulatedby both juvenile and adult birds. Body burdens ofcadmium, lead, and cesium-137 in adult birds were belowlevels associated with toxicity. Concentrations of selenium and mercury were observed at potentiallytoxic levels. Contaminants in eggshells andnestling feathers indicate exposure, however, thereis insufficient information to evaluate thetoxicological significance of this contamination.National Laboratory Oak Ridge National Laboratory is managed for the U.S. Department of Energy by Lockheed Martin Energy Research Corp. under contract DE-AC05-96OR22464     

Comparison of Canadian firefighters and healthy controls based on submaximal fitness testing and strength considering age and gender

Introduction. Few studies have addressed whether firefighters are fitter than the general population and possess sufficient levels of aerobic capacity and muscle strength to perform on-duty tasks in a safe and efficient manner, considering age and gender. We aimed to evaluate the fitness levels of Hamilton firefighters, and to determine the effects of age and gender. Methods. In total, 89 participants were recruited. The modified Canadian aerobic fitness test was used to determine participants’ estimated maximal oxygen consumption (V_O2max) levels. For upper and lower body strength levels, a calibrated J-Tech hand-held dynamometer and a National Institute for Occupational Safety and Health (NIOSH) lifting device was used respectively. Results. Firefighters’ mean (SD) V_O2max level was 40.30?±?6.25?ml·kg^?1·min^?1. Age proved to have a statistically significant impact on V_O2max (p?<?0.001). Gender displayed statistically significant effects on strength levels. Firefighters’ age was the only statistically significant independent variable, and accounted for 61.00% of the variance in firefighters’ aerobic capacity levels. Conclusions. Firefighters possessed somewhat similar aerobic capacities but much higher levels of body strength when compared with the general population. With age, firefighters’ aerobic capacities decreased; however, their upper and lower body strength levels remained the same.