Characterization of ACC deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of Brassica napus

One hundred Cu-resistant-endophytic bacteria were isolated from Cu-tolerant plants grown on Cu mine wasteland, of which, eight Cu-resistant and 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing endophytic bacteria were obtained based on the ACC deaminase activity of the bacteria and characterized with respect to metal resistance, production of ACC deaminase, indole-3-acetic acid (IAA) as well as siderophores and mineral phosphate solubilization. Ralstonia sp. J1-22-2, Pantoea agglomerans Jp3-3, and Pseudomonas thivervalensis Y1-3-9 with higher ACC deaminase activity (ranging from 213 to 370 μM α-ketobutyrate mg(-1)h(-1)) were evaluated for promoting plant growth and Cu uptake of rape grown in quartz sand containing 0, 2.5, and 5 mg kg(-1) of Cu in pot experiments. The eight bacteria were found to exhibit different multiple heavy metal resistance characteristics, to show different levels of ACC deaminase activity and to produce indole acetic acid. Seven bacteria produced siderophores and solubilized inorganic phosphate. Pot experiments showed that inoculation with the strains (J1-22-2, Jp3-3, and Y1-3-9) was found to increase the biomass of rape. Increases in above-ground tissue Cu contents of rape cultivated in 2.5 and 5 mg kg(-1) of Cu-contaminated substrates varied from 9% to 31% and from 3 to 4-fold respectively in inoculated-rape plants compared to the uninoculated control. The maximum Cu uptake of rape was observed after inoculation with P. agglomerans Jp3-3. The results show that metal-resistant and plant growth promoting endophytic bacteria play an important role in plant growth and Cu uptake which may provide a new endophytic bacterial-assisted phytoremediation of Cu-contaminated environment.     

Removal of 2-chlorophenol from water using rice-straw derived ash

Removal of 2-chlorophenol from water using rice-straw derived ash (RSDA) was evaluated in this study to compare with commercial activated carbon. RSDA was obtained by burning rice-straw at 400 °C and 700 °C for 1 h. This ash can provide a better adsorbent for 2-chlorophenol. The adsorption capacities of RSDA at 400 °C and 700 °C are 37 and 52 mg g?1 at pH 4, respectively, and decrease to 9.0 and 40 mg g?1 at pH 10. Adsorption of either neutral or anionic 2-cholorphenol by the RSDA are shown as L-shaped nonlinear isotherms, suggesting surface adsorption rather than partitioning is occurring. At higher-burning temperatures, the surface area, porosity, point of zero charge and aromaticity of the resultant RSDA increase, but the oxygen content and surface acidity decrease. The combined effects result in a higher 2-chlorophenol adsorption of RSDA at 700 °C, which shows a slight pH effect on the adsorption of 2-chlorophenol, due to the lower content of oxygen-containing functional groups. Oxygen-containing functional groups contribute to surface acidity and H-bonding sites for adsorbed water, which compromises the interaction between 2-chlorophenol and the adsorbents. Thus, it suggests that rice-straw derived carbon (RSDC) can be used as an effective low-cost substitute material for activated carbon for removal of chlorophenols from wastewater.     

粗MnSO4溶液中MnS2O6的去除条件研究

用软锰矿吸收SO2生成MnSO4时会伴随有副产物MnS2O6生成。产品中MnS2O6的存在会降低硫酸锰产品质量,同时会缓慢释放出SO2气体污染环境。本文用单因素实验方法研究了粗MnSO4溶液中MnS2O6的最优去除条件。研究结果显示,软锰矿用量为12.2 g,硫酸加入量为20 mL、反应温度100℃、反应时间3 h、搅拌速度500 r/min,MnS2O6的去除率可达91%以上。其最终产品能达到GB1622-1986一级品标准。     

淀粉-g-丙烯酸/凹凸棒土复合物制备及其吸附废水中NH_4~＋-N性能

采用反相乳液聚合法以凹凸棒土为原料,合成了新型NH4＋-N吸附剂淀粉-g-丙烯酸/凹凸棒土,并进行了氨氮吸附对比实验。结果表明：凹凸棒土氨氮单位吸附量为4.243 mg/g;淀粉-g-丙烯酸/凹凸棒土氨氮单位吸附量为5.301 mg/g,吸附能力比未改性的凹凸棒土提高了25%。淀粉-g-丙烯酸/凹凸棒土的氨氮吸附过程比凹凸棒土更符合Freundlich等温吸附模型。随着pH、温度的升高,凹凸棒土和淀粉-g-丙烯酸/凹凸棒土对NH4＋-N吸附量逐渐增大。     

酸性条件下剩余污泥中Ca2＋和Mg2＋溶出对磷回收的影响

为了在酸性条件下实现剩余污泥中磷的高效回收,对pH=3时剩余污泥水解酸化过程中氨氮、正磷酸盐和钙镁离子的溶出现象以及磷回收进行了研究分析。结果表明：当pH=3时,所溶出的氨氮、镁离子和钙离子与磷酸盐的摩尔比均大于1,能满足采用鸟粪石沉淀法或者羟磷灰石沉淀法回收磷的要求;但所溶出的钙镁离子的摩尔比大于1,会对鸟粪石沉淀法回收磷的顺利进行有较大影响;有无外加镁剂对磷回收率影响不大。采用改型后的镁型强酸性阳离子交换树脂进行离子交换可以得到较高纯度的鸟粪石沉淀产品,通过XRD检测其纯度为95％以上。     

Evaluation of geometrically personalized THUMS pedestrian model response against sedan–pedestrian PMHS impact test data

Objective: Evaluating the biofidelity of pedestrian finite element models (PFEM) using postmortem human subjects (PMHS) is a challenge because differences in anthropometry between PMHS and PFEM could limit a model's capability to accurately capture cadaveric responses. Geometrical personalization via morphing can modify the PFEM geometry to match the specific PMHS anthropometry, which could alleviate this issue. In this study, the Total Human Model for Safety (THUMS) PFEM (Ver 4.01) was compared to the cadaveric response in vehicle–pedestrian impacts using geometrically personalized models.

Methods: The AM50 THUMS PFEM was used as the baseline model, and 2 morphed PFEM were created to the anthropometric specifications of 2 obese PMHS used in a previous pedestrian impact study with a mid-size sedan. The same measurements as those obtained during the PMHS tests were calculated from the simulations (kinematics, accelerations, strains), and biofidelity metrics based on signals correlation (correlation and analysis, CORA) were established to compare the response of the models to the experiments. Injury outcomes were predicted deterministically (through strain-based threshold) and probabilistically (with injury risk functions) and compared with the injuries reported in the necropsy.

Results: The baseline model could not accurately capture all aspects of the PMHS kinematics, strain, and injury risks, whereas the morphed models reproduced biofidelic response in terms of trajectory (CORA score = 0.927 ± 0.092), velocities (0.975 ± 0.027), accelerations (0.862 ± 0.072), and strains (0.707 ± 0.143). The personalized THUMS models also generally predicted injuries consistent with those identified during posttest autopsy.

Conclusions: The study highlights the need to control for pedestrian anthropometry when validating pedestrian human body models against PMHS data. The information provided in the current study could be useful for improving model biofidelity for vehicle–pedestrian impact scenarios.     

吊拖船队通过限制性顺直航段安全限制因素研究

为了保障吊拖船队通过顺直航道的航行安全,充分考虑吊拖船队的柔性结构特征和航道条件,深入研究限制性航道顺直航段航道特点,运用船舶操纵与引航理论,建立以吊拖船队为研究对象的顺直航段航道宽度计算模型,从而确定针对航道宽度的吊拖船队通过顺直航段的安全限制因素,运用实船试验数据和现有航道维护尺度对建立的公式进行验算。提出了符合我国运河航道条件和吊拖船队航行特点的运河限制性航道顺直航段航道宽度计算公式,以期为保障吊拖船队在顺直航道安全航行提供理论支撑,弥补我国运河以吊拖船队为主要船型的航道技术尺度计算方面的不足。     

A comparative study of vocational education and occupational safety and health training in China and the UK

In order to enhance Chinese workers’ occupational safety awareness, it is essential to learn from developed countries’ experiences. This article investigates thoroughly occupational safety and health (OSH) in China and the UK; moreover, the article performs a comparison of Chinese and British OSH training-related laws, regulations and education system. The following conclusions are drawn: China’s work safety continues to improve, but there is still a large gap compared with the UK. In China a relatively complete vocational education and training (VET) system has been established. However, there exist some defects in OSH. In the UK, the employer will not only pay attention to employees’ physiological health, but also to their mental health. The UK’s VET is characterized by classification and grading management, which helps integrate OSH into the whole education system. China can learn from the UK in the development of policies, VET and OSH training.     

The impact of urban planning on land use and land cover in Pudong of Shanghai,China

Functional zones in cities constitute the most conspicuous components of newly developed urban areas,and have been a hot spot for domestic and foreign investors in China,which not only show the expanse of urban space accompanied by the shifts both in landscape(from rural to urban)and land use(from less extensive to extensive),but also display the transformation of regional ecological functions.By using the theories and methods of landscape ecology,the structure of landscapes and landscape ecological planning can be analyzed and evaluated for studying the urban functional zones‘layout.In 1990,the Central Govermment of China declared to develop and open up Pudong New Area so as to promote economic development in Shanghai.Benefited from the advantages of Shanghai‘s location and economy,the government of Pudong New Area has successively built up 7 different functional zones over the past decade according to their functions and strategic goals.Based on the multi-spectral satellite imageries taken in 1990,1997and 2000,a landscape ecolgy analysis was carried out for Pudong New Area of Sanghai ,supported by GIS technology.Green space(including croplands )and built-up areas are the major factors considered in developing urban landscape.This paper was mainly concerned with the different spatial patterns and dynamics of green space,built-up areas and new buildings in different functional zones,as influenced by different spatial patterns and dynamics of green space,built-up areas and new buildings in different functional zones,as influenced by different functional layouts and development strategies.The rapid urbanization in Pudong New Area resulted in a more homogeneous landscape.Agricultural landscape and suburban landscape were gradually replaced by urban landscape as the degree of urbanization increased.As a consequence of urbanization in Pudong,not only built-up patches,but also newly-built patches and green patches merged into one large patch,which should be attributed to the construction policy of extensive green space as the urban development process in Pudong New Area.The shape of green area of 7 functional zones became more and more regular because of the horticultural needs in Shanghai urban planning.Some suggestions were finally made for the study of future urban planning and layout.     

太湖梅梁湾水体中可见光的衰减、吸收及散射

对太湖梅梁湾沿岸带和敞水区的水体进行了光学特性研究。结果发现，沿岸带的水下光辐射衰减系数(Kd)值较敞水区小；400nm与500nm向上辐照度比值(Eu440／Eu550),沿岸带在1m左右最小,敞水区在0．5m左右最小;沿岸带的水面下向上辐照度与向下辐照度的比值(R)低于敞水区：440、550、670nm的后向散射与吸收的比值(b／a),在沿岸带的1m处最大,敞水区在0．5m处最大。这些结果说明,相对于敞水区而言,沿岸带的光衰减较弱,无机悬浮颗粒的含量较少,最大叶绿素a分布较深。