Daytime light intensity affects seasonal timing via changes in the nocturnal melatonin levels

Daytime light intensity can affect the photoperiodic regulation of the reproductive cycle in birds. The actual way by which light intensity information is transduced is, however, unknown. We postulate that transduction of the light intensity information is mediated by changes in the pattern of melatonin secretion. This study, therefore, investigated the effects of high and low daytime light intensities on the daily melatonin rhythm of Afro-tropical stonechats (Saxicola torquata axillaris) in which seasonal changes in daytime light intensity act as a zeitgeber of the circannual rhythms controlling annual reproduction and molt. Stonechats were subjected to light conditions simulated as closely as possible to native conditions near the equator. Photoperiod was held constant at 12.25 h of light and 11.75 h of darkness per day. At intervals of 2.5 to 3.5 weeks, daytime light intensity was changed from bright (12,000 lux at one and 2,000 lux at the other perch) to dim (1,600 lux at one and 250 lux at the other perch) and back to the original bright light. Daily plasma melatonin profiles showed that they were linked with changes in daytime light intensity: Nighttime peak and total nocturnal levels were altered when transitions between light conditions were made, and these changes were significant when light intensity was changed from dim to bright. We suggest that daytime light intensity could affect seasonal timing via changes in melatonin profiles. Professor Dr. E. Gwinner died on 07 September 2004.     

Assays with Daphnia magna and Danio rerio as alert systems in aquatic toxicology

For the evaluation and monitoring of the water quality, a series of methodologies, which have as basis an ample variety of bioindicators, may be applied. The aim of this research was to evaluate the use of ecotoxicity assays with Daphnia magna and Danio rerio as alert systems in water contaminated with toxic substances. Using two toxicity databases, the sensibility of those aquatic organisms to a wide variety of chemical products and elements and to some chemical categories was investigated. The relation between the reference dose for human oral chronic exposure (RfD) of all chemical products and the acute toxicity values for both bioindicators was also studied. Acute toxicity tests with D. magna respond to a larger variety of chemicals with a higher sensitivity than those with D. rerio. Although mammals, crustaceans and fish have different routes of exposure, target organs and toxic mechanisms, acute toxicity essays with fish and Daphnia may be used as an initial screening before mammal models are used.     

On the radiocesium carbonate barrier in organics-rich sediments of Lake Juodis, Lithuania

Radiocesium vertical profiles in organics-rich sediments of running shallow eutrophic Lake Juodis (Lithuania) were studied in relation to seasonal variations of vertical profiles (in water column and sediments) of standard variables (pH, redox potential, temperature, oxygen concentrations, conductivity). It is shown that the sedimentation rate, radiocesium mobility and its vertical profiles in sediments are controlled by the vital cycle (processes of the growth, accumulation and decomposition) of green algae covering the main bottom areas of the lake. It is also shown that calcite deposits are formed in the shallow bottom areas that are oxygenated throughout the year because of the photosynthetic activity of the green algae covering the sediment. Formation of the calcite coatings on freshly accumulated organics is remarkable for causing elevated densities of sediment solids in the upper part of the respective vertical profiles. These calcite deposits behave as a barrier for radiocesium backward flux to the bottom water making the respective bottom areas a radionuclide sink. Together with the jelly-structured sediments lying below these deposits, the calcite preserves the shape of the primary radiocesium vertical profiles formed due to free-ion diffusion after the deposition event. It was determined that bottom areas anaerobic in winter are the main radiocesium source in the water column and cause characteristic radiocesium redistribution in surface sediments.     

Intra-cultivar variability of the soil-to-grain transfer of fallout 137Cs and 90Sr for winter wheat

Differences between the root uptake of fallout radionuclides by different cultivars ('inter-cultivar' variability) growing on the same field may be influenced not only by genetic differences of the cultivars, but also by the spatial variability of the soil-to-grain transfer within the cultivation area of each cultivar. This 'intra-cultivar' variability was investigated in 2001 and 2002 for 137Cs and 90Sr using three winter wheat cultivars with four replicates for each cultivar at three different sites in Bavaria, Germany. The intra-cultivar variability proved to be in the same range as the inter-cultivar variability which was determined earlier at the same sites for both radionuclides. An ANOVA of the 137Cs data set revealed that the variability of the 137Cs soil-to-grain transfer was caused by the soil and climate (year) at the field sites and the interaction of cultivar and field. A significant contribution of the factor 'cultivar' alone to the variability could not be detected. This may be due to the complex environmental conditions to which plants are exposed in field experiments. To find wheat cultivars with minimal uptake of fallout radionuclides it may be better to examine the molecular mechanisms of their root uptake in order to identify targets for breeding "safer" plants.     

Trace element levels in foetus-mother pairs of short-beaked common dolphins (Delphinus delphis) stranded along the French coasts

Tissues of foetus-mother pairs of common dolphins (Delphinus delphis) stranded along the French coasts (Bay of Biscay and English Channel) were analysed for their Cd, Cu, Hg, Se and Zn contents. In the kidneys, foetal Cd levels were extremely low, and strong relationships between Cu and Zn suggested the involvement of metallothioneins since early foetal life. The results also indicated a limited maternal transfer of Hg during pregnancy since levels in the tissues of foetuses were below 1 microg g(-1) w.wt. However, hepatic Hg levels in foetuses increased with body length, and were also proportionate to maternal hepatic, renal and muscular Hg levels. Lastly, affinities between Hg and Se in tissues would participate in Hg neutralisation in both mothers--through tiemannite granules--and fetuses--through reduced glutathione--counteracting the toxic effects linked to the particularly high quantities of methyl-Hg to which marine mammals are naturally exposed.     

Origin of major ions in monthly rainfall events at the Bamenda Highlands, North West Cameroon

Rainwater characteristics can reveal emissions from various anthropogenic and natural sources into the atmosphere. The physico-chemical characteristics of 44 monthly rainfall events （collected between January and December 2012） from 4 weather stations （Bamenda, Ndop plain, Ndawara and Kumbo） in the Bamenda Highlands （BH） were investigated. The purpose was to determine the sources of chemical species, their seasonal inputs and suitability of the rainwater for drinking. The mean pH of 5 indicated the slightly acidic nature of the rainwater. Average total dissolved solids （TDS） were low （6.7 mg/L）, characteristic of unpolluted atmospheric moisture/air. Major ion concentrations （mg/L） were low and in the order K＋ 〉 Ca2＋ 〉 Mg2~ 〉 Na＋ for cations and NO3 〉〉 HCO3 〉 SO] 〉 CI- 〉 PO3- 〉 F- for anions. The average rainwater in the area was mixed Ca-Mg-SO4-CI water type. The CI-/Na＋ ratio （1.04） was comparable to that of seawater （1.16）, an indication that N a＋ and CI originated mainly from marine （Atlantic Ocean） aerosols. High enrichments of Ca2＋, Mg2＋ and SO2- to Na＋ ratios relative to seawater ratios （constituting 44% of the total ions） demonstrated their terrigenous origin, mainly from Saharan and Sahelian arid dusts. The K＋/Na＋ ratio （2.24）, which was similar to tropical vegetation ash （2.38）, and NO3 was essentially from biomass burning. Light （〈 100 mm） pre-monsoon and post-monsoon convective rains were enriched in major ions than the heavy （〉 100 mm） monsoon rains, indicating a high contribution of major ions during the low convective showers. Despite the acidic nature, the TDS and major ion concentrations classified the rainwater as potable based on the WHO guidelines.     

The Influence of Linear Elements on Plant Species Diversity of Mediterranean Rural Landscapes: Assessment of Different Indices and Statistical Approaches

This paper mainly aims to study the linear element influence on the estimation of vascular plant species diversity in five Mediterranean landscapes modeled as land cover patch mosaics. These landscapes have several core habitats and a different set of linear elements -habitat edges or ecotones, roads or railways, rivers, streams and hedgerows on farm land- whose plant composition were examined. Secondly, it aims to check plant diversity estimation in Mediterranean landscapes using parametric and non-parametric procedures, with two indices: Species richness and Shannon index.Land cover types and landscape linear elements were identified from aerial photographs. Their spatial information was processed using GIS techniques. Field plots were selected using a stratified sampling design according to relieve and tree density of each habitat type. A 50×20 m² multi-scale sampling plot was designed for the core habitats and across the main landscape linear elements. Richness and diversity of plant species were estimated by comparing the observed field data to ICE (Incidence-based Coverage Estimator) and ACE (Abundance-based Coverage Estimator) non-parametric estimators.The species density, percentage of unique species, and alpha diversity per plot were significantly higher (p < 0.05) in linear elements than in core habitats. ICE estimate of number of species was 32% higher than of ACE estimate, which did not differ significantly from the observed values. Accumulated species richness in core habitats together with linear elements, were significantly higher than those recorded only in the core habitats in all the landscapes. Conversely, Shannon diversity index did not show significant differences.     

PAHs Contamination in Bank Sediment of the Yamuna River, Delhi, India

This study was performed to elucidate the distribution, concentration trend and possible sources of PAHs in bank sediment of river Yamuna in Delhi, India. The levels of 16 priority polycyclic aromatic hydrocarbons (PAHs) were analyzed during pre-monsoon, monsoon and post-monsoon seasons in the sediment fraction < 53 μm. Reference standards and internal standards were used for identification and quantification of PAHs by HPLC. The sum of 16 PAH compounds ranged from 4.50 to 23.53 μg/g with a mean concentration of 10.15 ± 4.32 μg/g (dry wt.). Among 5 sites studied, the site, Income Tax Office (ITO) was found to be the hotspot attaining highest concentration. Predominance of 2–4 ring PAHs suggests a relatively recent local sources of PAHs in the study area. Moreover, molecular indices based source apportionment also illustrates pyrogenic source fingerprint of PAHs. No significant temporal trend was observed.     

Use of Artificial Roost Structures by Bats at the Indianapolis International Airport

From 1992–1996, 3204 artificial roosts of 9 types were placed in woodlots near Indianapolis International Airport in an effort to provide habitat for the federally-endangered Indiana myotis (Myotis sodalis) and to determine the feasibility of using these structures to manage bats in a rapidly developing suburban area. We surveyed these structures at least annually during 1992–1999 and found only northern myotis (Myotis septentrionalis) regularly using the structures. Four other species were occasionally found using structures including big brown bats (Eptesicus fuscus, n = 14 individuals), little brown myotis (Myotis lucifugus, n = 2), Indiana myotis (Myotis sodalis, n = 2), and one silver-haired bat (Lasionycteris noctivagans). Single, triple, and Missouri-style batboxes were almost always used, rather than the six other types of experimental roosts that had been in place. However, after 10 years in place, it appears that Indiana bats are acclimated to boxes, as 6 of them were being used rather regularly by Indiana myotis. Bat boxes can provide roosting habitat for some species under conditions where few suitable roosts exist, but assuring an abundance of natural habitats is usually more desirable for conservation of tree-roosting bats.